1726 TTAAVDMPRSTP(3) HESFWYLPHQSY(1) LLADTTHHRPWT(8) QSNYPRASYVFQ(1) KSLSRHDHIHHH(2) MPWAHRAPQGIA(1) 6 Phage display (common panning) 6 DOI:10.1021/cm071515t (001)-oriented TiO2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) A 0.2% TBST wash solution was used to remove phages with low binding affinity to titania during the first four rounds, whereas a more stringent 0.5% TBST wash solution was used for the fifth round. A additional round of screening were conducted with a 0.5% TBST wash solution (for a total of six rounds of biopanning). 1727 LLADTTHHRPWT(2) SHAFTASPRYLH(4) KSLSRHDHIHHH(6) YAKSPPTPYYTP(4) LAPKPFEPRYTR(1) 5 Phage display (common panning) 7 DOI:10.1021/cm071515t (001)-oriented TiO2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) A 0.2% TBST wash solution was used to remove phages with low binding affinity to titania during the first four rounds, whereas a more stringent 0.5% TBST wash solution was used for the fifth round. Two additional rounds of screening were conducted with a 0.5% TBST wash solution (for a total of seven rounds of biopanning). 1728 KSLSRHDHIHHH(17) SRPSRQPSASPT(3) 2 Phage display (common panning) 8 DOI:10.1021/cm071515t (001)-oriented TiO2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) A 0.2% TBST wash solution was used to remove phages with low binding affinity to titania during the first four rounds, whereas a more stringent 0.5% TBST wash solution was used for the fifth round. Three additional rounds of screening were conducted with a 0.5% TBST wash solution (for a total of eight rounds of biopanning). 1729 DPIYALSWSGMA(1) TMATYVNQSLTG(1) SVSLPYANLATH(1) SLYNTAASHVPT(1) DLNTNRTGMVLH(2) NYLHNHPYGTVG(1) LTPTSRPTPYPA(1) KSLSRHDHIHHH(9) NFRPVTAMPRLD(1) NINANAAQIKRH(1) 10 Phage display (common panning) 6 DOI:10.1021/cm071515t (001)-oriented TiO2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) A 0.2% TBST wash solution was used to remove phages with low binding affinity to titania during the first four rounds, whereas a more stringent 0.5% TBST wash solution was used for the fifth round. A additional round of screening were conducted with a 0.8% TBST wash solution (for a total of six rounds of biopanning). 1730 TQHLSHPRYATK(9) YAKSPPTPYYTP(8) KSLSRHDHIHHH(5) 3 Phage display (common panning) 7 DOI:10.1021/cm071515t (001)-oriented TiO2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) A 0.2% TBST wash solution was used to remove phages with low binding affinity to titania during the first four rounds, whereas a more stringent 0.5% TBST wash solution was used for the fifth round. Two additional rounds of screening were conducted with a 0.8% TBST wash solution (for a total of six rounds of biopanning). 1731 APPGHHHWHIHH MSASSYASFSWS KPSHHHHHTGAN 3 Phage display (common panning) 3 PMID:12908327 Silica, Si Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 1732 MSPHPHPRHHHT MSPHHMHHSHGH LPHHHHLHTKLP APHHHHPHHLSR RGRRRRLSCRLL 5 Phage display (common panning) 4 PMID:12908327 Silica, Si Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 1733 LKAHLPPSRLPS 1 Phage display (common panning) PMID:16601154 Gold Not determined. Not determined. Not determined. X12 M13 phage display library 1734 RKLPDAPGMHTW(34) LDTTNVSGPMSS(1) SYRLPVYLHALL(1) SDPNQDWRRTTP(1) LPSQLLSQVNLT(1) LCANNTTSVHPP(1) MQMEGKPTLTLR(1) STLKNPINLLAN(2) QDMIRTSALMLQ(1) SCHVWYDSCSSP(1) 10 Phage display (common panning) 3 PMID:14624545 Titanium, Ti Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) After three rounds of panning procedures carried out against Ti particles, we observed that 33 of 43 phages displayed on their surfaces peptides having the identical sequence. 1735 NFMSLPRLGHMH TSNAVHPTLRHL 2 Phage display (common panning) PMID:19422199 Pd wire Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Both peptide sequences possessed basic pI values; however, the peptide TSNAVHPTLRHL sequence possessed more basic and hydroxyl-containing residues, comparatively. Additionally, theoretical modeling of the peptide Pd surface binding capabilities suggests that the two histidine residues bind to the materials surface in a pinched arrangement, which will likely lead to open interaction sites between the solution and the metallic surface. 1736 TGTSVLIATPYV 1 Phage display (common panning) PMID:20083240 Gold Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Metallic gold powder in an Eppendorf tube was directly used as the target material for isolation of binding phages instead of a substrate coated with the target material or a solution of phage with a target attached by affinity tags. 1737 LNAAVPFTMAGS 1 Phage display (common panning) 4 PMID:20627315 Titanium dioxide, TiO2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) First, three rounds of phage display selection were performed in an Eppendorf PP tube containing a 5-mm-diameter and 2 mm amorphous-TiO2-coated disk. Secondly, an additional round of selection was performed on newly shaped surfaces. The TiO2-binding peptide sequence (LNAAVPFTMAGS) occurred in 50% of the clones. 1738 MTWDPSLASPRS 1 Phage display (common panning) 4 PMID:20627315 Stainless steel Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) First, three rounds of phage display selection were performed in an Eppendorf PP tube containing a 5-mm-diameter and 2 mm thick stainless steel. Secondly, an additional round of selection was performed on newly shaped surfaces. The stainless steel-binding peptide sequence (MTWDPSLASPRS) occurred in 70% of the clones. 1739 YQLRPNAESLRF 1 Phage display (common panning) 4 PMID:20627315 Bulk steel (edges) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) A dominant peptide sequence for recognition of the steel of the edges was highlighted (YQLRPNAESLRF). 1740 AGETQQAM 1 Phage display (common panning) 3 PMID:20383127 Iridium oxide, IrO2 Not determined. Not determined. Not determined. X8 M13 phage display library 1741 MFLRAG LHADVW QLYMKG WAMSEV EPGLDR SFNILR PVQASP DSASHS SPCRGL GWWPHR 10 Phage display (subtractive panning) 3 PMID:7892246 Capsid protein Not determined. Not determined. Not determined. f3-6mer phage display library (X6) The library had been incubated with a buffer containing BSA, before panning, to eliminate phage that bind BSA. The phage library was screened against truncated HBcAg (aa 3-148). The washing buffer used during affinity purification contained 0.15M NaCl. 1742 LLGRMK(15)[0.17 ± 0.01, 0.22 ± 0.01] YLLRFR(11)[1.53 ± 0.10, ND] LLGRLK(6)[1.13 ± 0.05, ND] LLGRFK(2)[0.64 ± 0.03, ND] LLGRFR(2)[NT] LLGRLR(1)[NT] LLGRMR(1)[NT] SLWKWK(1)[NT] WTFLRG(1)[NT] L-L-G-R-[MFL]-[KR] 9 Phage display (subtractive panning) 3 PMID:7892246 Capsid protein Not determined. Not determined. Not determined. f3-6mer phage display library (X6) Binding assay Relative dissociation constants (KdRel, μM) of the phage-truncated HBcAg complexes and the phage-full-length HBcAg complexes were shown, respectively. ND represents not determined. NT denotes not tested. The library had been incubated with a buffer containing BSA, before panning, to eliminate phage that bind BSA. The phage library was screened against truncated HBcAg (aa 3-148). The washing buffer used during affinity purification contained 0.5M NaCl. In the first round of panning, 0.001% of phage bound to the membrane and this binding increased to 1% in the third panning. Of 46 independent clones, the sequence LLGRMK predominated. Six sequences are considered unrelated and not given in the original paper. LLGRMK-bearing phage bound to full-length HBcAg (aa 3-183) as efficiently as to truncated HBcAg but did not bind to heat-denatured, truncated HBcAg. The sequence LLGRMK could represent one loop of the binding site of an antibody to HBcAg, but more interestingly it may resemble a region of the HBsAg polypeptide that contacts the nucleocapsid in the intact virion. 1743 ANGCCD HAILNI ESQSVL RVADIV SKGTVA DVSIGV RYLVLE NINSPV IWLSNG WPRGAG 10 Phage display (subtractive panning) 3 PMID:7892246 Bovine serum albumin (BSA) Not determined. Not determined. Not determined. f3-6mer phage display library (X6) The library had been incubated with a buffer containing BSA, before panning, to eliminate phage that bind BSA. Thus, the peptides here are considered to be random sequences. 1744 CFDYAPYVSAVDDIC LSAVDDLATVVYGSR AGCTALSAVDDVPGC ASSAVDDAPWAVITF VGLLSVEELVPGGAA GSFSAEHFLDDFAIW RNVPPIFNDVYWLAF GGVSAVPFMDYFPSW [ST]-A-V-D-D, S-A-V-P-x(2)-D, S-A-x(4)-D 8 Phage display (common panning) 2-4 PMID:10456916 Anti-RESA monoclonal antibody MAb 18/2 Ring-infected erythrocyte surface antigen Not determined. Not determined. f3-15mer phage display library (X15) Most phage isolated from this library bound to MAb 18/2-coated ELISA plates. The binding of representative peptides, CFDYAPYVSAVDDIC and GSFSAEHFLDDFAIW, to MAb 18/2 has been confirmed. 1745 QTAVDDAEAAAKCRHCI QDETRSAVDSIPQAHCS GLKNCTVQPWDATDVCD QISAVS LPAQPNACWPTMTPLCA YVGSQSEDRDMSCGHCS QQNDLLVYSAVPLSDCK ASAAEGDD [ST]-A-V-D-D, S-A-V-P-x(2)-D, S-A-x(4)-D 8 Phage display (common panning) 2-4 PMID:10456916 Anti-RESA monoclonal antibody MAb 18/2 Ring-infected erythrocyte surface antigen Not determined. Not determined. X15CX phage display library Interestingly, sequences of two peptides QISAVS and ASAAEGDD align with the second motif only if the phage protein backbone of gpVIII to which the peptides are fused is considered to contribute to the binding. Most phage isolated from this library bound to MAb 18/2-coated ELISA plates. In contrast, phage lacking a peptide (wild-type M13 phage) did not bind to MAb 18/2, and one phage clone with the insert sequence YVGSQSEDRDMSCGHCS, which lacks the consensus motif, also did not bind. RESA protein inhibits binding of MAb 18/2 to selected phage. The binding of a representative peptide, GLKNCTVQPWDATDVCD, to MAb 18/2 has been confirmed. 1746 LWVGGGRNA(6) 1 Phage display (common panning) 3 PMID:10867191 Grass carp hemorrhage virus Not determined. Not determined. Not determined. X9 fUSE5 phage display library To remove weakly or nonspecifically bound peptidea??a Sixteen clones which inhibited the replication of GCHV in a grass carp kidney cell line were selected. The TCID 50 of GCHV was decreased over 10 000×. Six clones having the strongest inhibitory effect shared the same DNA sequence, with a deduced amino acid sequence of NH(2)-Leu-Trp-Val-Gly-Gly-Gly-Arg-Asn-Ala-COOH. 1747 CTLTTKLYC CQLGGPSHC CQKGGPSHC CQHEMPSKC 4 Phage display (common panning) 1 PMID:12021868 Newcastle disease virus, NDV Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. 1748 CTLTTKLYC CTLTTKAYC CTLTTPELC CTPTPRLYC CTPSPRLYC CTVWTSEHC CQLGGPSHC CQKGGPSDC CQKGGPSHC 9 Phage display (common panning) 2 PMID:12021868 Newcastle disease virus, NDV Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. 1749 CTLTTKLYC CNLTTKLYC 2 Phage display (common panning) 3 PMID:12021868 Newcastle disease virus, NDV Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Thirty-nine out of 40 phages analyzed from the third round carried the sequence TLTTKLY and only one related sequence was identified in which its first residue, Thr (T), was substituted by Asn (N). An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. 1750 CHPQFEALC 1 Phage display (common panning) 3 PMID:12021868 Streptavidin Biotin 1DF8,1LUQ,1MEP,1MK5,1N43,1N9M,1NDJ,1NQM,1STP,1SWD,1SWE,1SWG,1SWK,1SWN,1SWP,1SWR,1SWT,2F01,2GH7,2IZF,2IZG,2IZH,2IZI,2IZJ,2RTD,2RTE,2RTF,2RTG,2Y3F,3MG5, Not determined. Ph.D.-C7C phage display library (CX7C) Biopanning gave a consensus sequence of HPQFEAL, which was about 70% of the total phages screened from the third round of panning.