151 CXnC phage display library pool (n = 3-12) 3-12 Kim D. Janda Completely random Circular 152 CX7C phage display library 7 4.5e9 Erkki Koivunen (Division of Biochemistry, University of Helsinki, Helsinki, Finland) Completely random NNK Circular The library was constructed on fUSE5 vector. 153 X3CX7CX3 phage display library 13 5.0e8 Yuan Wang Semi-random Circular To construct the vector pFuse8, M13 gene 8 was amplified using the genomic DNA of VCSM13 phage as template. Random peptides fused with pVIII assume a form of X3CX7CX3, where X stands for any amino acids. Two cysteine residues frank the 7 random amino acids in the middle. 154 X10 phage display library 10 1.2e9 Alessandro Pini Completely random Linear The decapeptide library DIP3 expressed on the minor phage coat protein pIII of M13 bacteriophage was constructed modifying the vector pDN332. 155 Substrate phage display library based on a modified pH0508b phagemid 5 3.6e6 1.8e8 Sylvain M. Cloutier Completely random NNS Linear 156 X15 phage display library 15 Sparks A B Completely random Linear 157 XCX15 phage display library 16 4e10 Lori Bonnycastle(Simon Fraser University,Burnaby,BC,Canada) Semi-random Linear 158 (X)7LXXLL(X)7 library 16 1.5e8 Donald P. Mcdonnell Semi-random NNK Linear The library in the format of (X)7LXXLL(X)7 was constructed essentially with the M13 phage-based cloning vector mBAX. 159 fTC-LIB-N6 phage display library 6 Marc Navre Completely random NNK Linear 160 X9 phage display library 9 Al Jesaitis, Montana State University Completely random Linear 161 X15 phage display library 15 Nishi, T Completely random Linear 162 fUSE-His-N6 phage display library 6 Shuichi Ohkubo Completely random NNK Linear A random hexamer substrate phage display library with a (His)6 at the NH2-terminal of pIII protein of filamentous phage (fUSE5) has been constructed. 163 CTLA-4 (X3-RGD-X3) scaffold library 6 Hennie R. Hoogenboom Semi-random Linear The complementarity determining region 3 (CDR3) loop of the CTLA-4 extracellular domain was evaluated as a permissive site. Authors replaced the nine amino acid CDR3-like loop of CTLA-4 with the sequence XXX-RGD-XXX (where X represents any amino acid). 164 RRT-SH3 phage display library 6 Kalle Saksela Completely random Linear 165 CX8C phage display library 8 Erkki Koivunen (Division of Biochemistry, University of Helsinki, Helsinki, Finland) Completely random NNK Circular The phage display peptide library is constructed in the vector fUSE5-phage. 166 CX10C phage display library 10 Erkki Koivunen (Division of Biochemistry, University of Helsinki, Helsinki, Finland) Completely random NNK Circular The phage display peptide library is constructed in the vector fUSE5-phage. 167 CX3CX3CX3C phage display library 9 Erkki Koivunen (Division of Biochemistry, University of Helsinki, Helsinki, Finland) Semi-random NNK Circular The phage display peptide library is constructed in the vector fUSE5-phage. 168 CX3CX4CX2C phage display library 9 Erkki Koivunen (Division of Biochemistry, University of Helsinki, Helsinki, Finland) Semi-random NNK Circular The phage display peptide library is constructed in the vector fUSE5-phage. 169 PDL-10R phage display library 10 Christian, R. B. Semi-random NNK Linear PDL-10R was constructed in fUSE-5 vector (kindly provided by Dr. George Smith, University of Missouri, Columbia, MO). 170 PDL-10Y phage display library 9 Christian, R. B. Semi-random NNK Linear PDL-10Y was constructed in fUSE-5 vector (kindly provided by Dr. George Smith, University of Missouri, Columbia, MO). The library contained a tyrosine (TAT) at the third position of the insert sequence. 171 X2CX14CX2 phage display library 16 Erkki Koivunen (Division of Biochemistry, University of Helsinki, Helsinki, Finland) Semi-random Circular 172 X2CX18 phage display library 20 Erkki Koivunen (Division of Biochemistry, University of Helsinki, Helsinki, Finland) Semi-random Linear 173 X8 phage display library 8 Quanxi Li Completely random NNK Linear A random octapeptide library was prepared by cloning chemically-synthesized degenerated oligos into monovalent displayed phage antibody expressing vector, which is derived from pComh3H. 174 X15 phage display library 15 L. Jespers, Leuven, Belgium Completely random Linear 175 M13lib phage display library 9 Juergen Hammer Completely random NNS Linear MDstufferbb DNA was digested to completion with Sad and KpnI, and the stuffer was removed by gel electrophoresis to reduce any background.