151 CTALMSASC(3) 1 Phage display (common panning) 4 PMID:15863836 Pancreatic triacylglycerol lipase Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) In each round, bound clones were sequentially eluted with glycine-HCl, porcine pancreatic lipase and tetrahydrolipstatin. Four rounds of biopanning were performed. With the exception of the first round of each selection protocol, only the phages collected in the last elution step were amplified for the next round of biopanning. 152 CPPSYNGKC(3) CPDANRINC(1) CSQAPTPAC(1) CTPTSPAMC(1) CQPHPGQTC(1) 5 Phage display (common panning) 4 PMID:15863836 Pancreatic triacylglycerol lipase Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) In each round, bound clones were eluted with tetrahydrolipstatin. Four rounds of biopanning were performed. With the exception of the first round of each selection protocol, only the phages collected in the last elution step were amplified for the next round of biopanning. 153 CSQLQTTKC(1) 1 Phage display (common panning) 4 PMID:15863836 Pancreatic triacylglycerol lipase Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) In each round, bound clones were eluted with porcine pancreatic lipase. Four rounds of biopanning were performed. With the exception of the first round of each selection protocol, only the phages collected in the last elution step were amplified for the next round of biopanning. 154 SLFDTLA(10) SVFDRLA(3) LYAAINL(2) LPSFRAL(1) SPQLSKL(1) ALNLGSR(1) AINLGHR(1) AVRPVTP(1) PEKQAAR(1) GPPPKSH(1) TLFDTLK(1) TLFDMLR(1) TLFDVFR(1) SVFDTLA(1) AIWQKSW(1) SFHTFLQ(1) 16 Phage display (common panning) 3 PMID:16006240 Anti-EcA polyclonal antibody L-asparaginase 2 Not determined. Not determined. Ph.D.-7 phage display library (X7) 155 WSAPVLMGTVPP(8) QLNKLQIPLSII(2) RKVPLLSGTLPQ(1) QEPLMGTVPIRA(1) THNHQVPLAIIS(1) TMPWNQSALTLI(1) TLQQNMPLALVW(1) DPEQPAPLFLVS(1) APIQAHPLGLIR(1) PSIIGGSSVDLV(1) IDIINPAQNRLR(1) 11 Phage display (common panning) 3 PMID:15560754 Anti-NPS polyclonal antibody Neutral polysaccharides, NPS Not determined. Not determined. Ph.D.-12 phage display library (X12) 156 GPEDTSRAPENQQKTFHRRW(5) 1 Phage display (common panning) 5 PMID:15585132 Langerhans cells Not determined. Not determined. Not determined. X20 fAFF1 phage display library 157 ELRWDLTDIYNL(5) SWNSVDIWHMSS(4) W-x(3)-D-I 2 Phage display (common panning) 4 PMID:15483241 Anti-lycoprotein G monoclonal antibody MnAb1 Glycoprotein G Not determined. Not determined. Ph.D.-12 phage display library (X12) Conserved sequence, WxxxDI, aligned between aa 14 and 19 of the mature glycoprotein. 158 SWNSVDIWHMSS(4) SWSTLDIWSIPH(2) W-x(3)-D-I 2 Phage display (common panning) 4 PMID:15483241 Anti-lycoprotein G monoclonal antibody MnAb2 Glycoprotein G Not determined. Not determined. Ph.D.-12 phage display library (X12) Conserved sequence, WxxxDI, aligned between aa 14 and 19 of the mature glycoprotein. 159 ESLRWDQRIG(9) EFLGWGKAV(8) EVFRWSAASV(8) EEMRWGRLLS(5) NETRRWDILI(5) GGEEWFRWA(5) KDETWRWGR(4) LLRWGVSAT(4) EILRWGAIAP(3) VGRWGDFWL(3) PELWHTPV(3) VEMMRWGQTA(3) TEAFRWDMRA(2) LEHFRWMKPI(2) DYVCWWKASV(2) DGRWGDFWL(2) EWLRWLIYP(1) FCAVRWGLPC(1) LLRWGGLAV(1) SPRRSERLGW(1) ELFRWDTTHT(1) RIENFRWQRP(1) DSLRWGKVPV(1) ELAHPFWERY(1) SPTWRWAPY(1) WFRWGTAPAP(1) GYLRWHVPHS(1) LEQMRWMKHT(1) AELFRWGSSA(1) PQWFSWWRPA(1) 30 Phage display (common panning) 3 PMID:15893405 Anti-ACTH monoclonal antibody A2H8 Corticotropin Not determined. Not determined. X10 phage display library 160 SYGHPLGKPH(13) TMRWGFPVGR(5) SSGPPRAPGG(4) YTWVAVQSSR(3) HWGWPLGRLT(3) VWYQFPVGK(2) FGTPIGKPLR(2) FSAPVGKPGK(2) SYGRPLGKRL(2) GFGWPVGLKV(1) 10 Phage display (common panning) 3 PMID:15893405 Anti-ACTH monoclonal antibody 57 Corticotropin Not determined. Not determined. X10 phage display library 161 YGGEGLVSWG(2) YLENLSLPNR(2) SYGHPLGLPQ(1) WGSPVGLGH(1) FSAPVGLPGL(1) 5 Phage display (common panning) 3 PMID:15893405 Anti-ACTH monoclonal antibody FZ10-A32 Corticotropin Not determined. Not determined. X10 phage display library 162 WIATWQDDGYMY[0.704] 1 Phage display (common panning) PMID:15047171 Geminin Not determined. Not determined. Not determined. X12 phage display library ELISA The absorbance value (A) at 410 nm was determined by subtraction of the raw A of the GST plates from that of the GST–Geminin plates. Data shown were reproduced from the graph. 163 SFDPGLF(3) VEFPVSD(2) SIEFPTS(1) IEWPVSD(1) TIEFPVA(1) YAPEFPL(1) RHTMIIP(1) TIIFPVE(1) KWHWSDS(1) RHTMINP(1) NEFPVS(1) 11 Phage display (common panning) 3 PMID:15774614 Anti-GPIIIa49-66 polyclonal antibody Integrin beta-3 Not determined. Not determined. Ph.D.-7 phage display library (X7) 164 SHWETPLLSWFF SICDSHHRYSWL NHDYWYWLFENV WHSDMEWWYLLG WHVDETWLLMLT WHDPTPWWSWEI GNWMDFHLQRTA 7 Phage display (common panning) 4 PMID:15197767 Vascular endothelial growth factor receptor 1, VEGFR-1 Vascular endothelial growth factor A, VEGF-A 1FLT,1QTY, Not determined. Ph.D.-12 phage display library (X12) Peptide WHSDMEWWYLLG almost abolished VEGF binding to receptor Flt-1 in vitro. 165 TYPIPIR(3) TYPIPFR(3) TYPVPHR(1) TYPLPIR(11) TYPPPTR(1) T-Y-P-x-P-x-R 5 Phage display (common panning) 4 PMID:14532093 Bacillus anthracis Spore Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 166 NIMRNTW(4) AVPRASF(4) LGSYNNA(2) 3 Phage display (subtractive panning) 5 PMID:16599583 Lipopolysaccharide, LPS Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) The phage library was first incubated in a well not coated with LPS for 1 h at room temperature, upon which the unbound fraction was transferred to LPS-coated wells. 167 ASFPPAF(4) SSHTISF(2) 2 Phage display (subtractive panning) 5 PMID:16599583 Lipopolysaccharide, LPS Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) The panning procedure was the same as MS00166, except that an additional incubation step with LPS-O111 was added in each panning, and the unbound fraction was used for panning with LPS-O55. 168 RDFYYN(5)[+][NT] HDFFFN(2)[+][5.1 ± 0.3] WDLWVN(1)[+][3.4 ± 0.2] PGRYIN(1)[+][5.7 ± 1.1] NNYFFV(1)[+][0.006] GSGSIN(1)[+][0.9 ± 0.1] MPSYIN(1)[+][3.5 ± 0.2] PPAYFN(1)[+][1.8 ± 0.5] PDLYIN(1)[+][4.5 ± 0.1] MPQYVN(1)[+][2.0 ± 0.6] RGFYYN(1)[+][6.6 ± 1.6] NNYFFN(1)[+][6.6 ± 1.0] ARMYIN(1)[+][4.0 ± 0.2] DDTYNS(1)[+][1.8 ± 0.2] RGLYYN(1)[+][5.6 ± 0.1] 15 Phage display (common panning) 4 PMID:7499368 Cardiac glycoside digoxin Anti-digoxin antibody 26-10 Fab Not determined. Not determined. X6 phage display library ELISA,Saturation equilibrium assay Secreted Fab was tested using an ELISA with goat anti-mouse Fab, digoxin-BSA, and the congener-BSA used for selection as coating antigens. All clones were positive for Fab. Besides the designation + or - refers to whether the clone binds to the congener used in selection. Affinities (Ka,e-9 M-1) for digoxin of Fab 26 -10 and mutants (randomly mutated at positions H30 -35 inclusive) selected from the bacteriophage library. Affinities were measured using an equilibrium saturation method with filtration through glass fiber filters for separation of bound and free ligand, except that Fab was immobilized on the filter using goat anti-mouse Fab. 169 PSFYYN(1)[+][21.7 ± 8.1] RDFYYN(18)[+][9.7 ± 3.8] 2 Phage display (common panning) 4 PMID:7499368 16-acetylgitoxin Anti-digoxin antibody 26-10 Fab Not determined. Not determined. X6 phage display library ELISA,Saturation equilibrium assay Secreted Fab was tested using an ELISA with goat anti-mouse Fab, digoxin-BSA, and the congener-BSA used for selection as coating antigens. All clones were positive for Fab. Besides the designation + or - refers to whether the clone binds to the congener used in selection. Affinities (Ka,e-9 M-1) for digoxin of Fab 26 -10 and mutants (randomly mutated at positions H30 -35 inclusive) selected from the bacteriophage library. Affinities were measured using an equilibrium saturation method with filtration through glass fiber filters for separation of bound and free ligand, except that Fab was immobilized on the filter using goat anti-mouse Fab. 170 RDFYYN(4)[+] RRGIVN(2)[+] SKRYIN(1)[+] SMQWFN(1)[+] KDMYIN(1)[+] PFRFIN(1)[+] 6 Phage display (common panning) 5 PMID:7499368 Gitoxin Anti-digoxin antibody 26-10 Fab Not determined. Not determined. X6 phage display library ELISA Secreted Fab was tested using an ELISA with goat anti-mouse Fab, digoxin-BSA, and the congener-BSA used for selection as coating antigens. All clones were positive for Fab. Besides the designation + or - refers to whether the clone binds to the congener used in selection. 171 RDFYYN(7)[+][NT] GERFFN(2)[+][4.3 ± 0.6] SKRYIN(1)[+][0.3 ± 0.03] 3 Phage display (common panning) 6 PMID:7499368 Gitoxin Anti-digoxin antibody 26-10 Fab Not determined. Not determined. X6 phage display library ELISA,Saturation equilibrium assay Secreted Fab was tested using an ELISA with goat anti-mouse Fab, digoxin-BSA, and the congener-BSA used for selection as coating antigens. All clones were positive for Fab. Besides the designation + or - refers to whether the clone binds to the congener used in selection. Affinities (Ka,e-9 M-1) for digoxin of Fab 26 -10 and mutants (randomly mutated at positions H30 -35 inclusive) selected from the bacteriophage library. Affinities were measured using an equilibrium saturation method with filtration through glass fiber filters for separation of bound and free ligand, except that Fab was immobilized on the filter using goat anti-mouse Fab. NT denoted not tested. 172 SHSYIN(3)[+] RTRYIN(1)[+] TRYWFN(1)[+] PWTLLN(1)[+] SNLEDS(1)[-] SGRPPN(1)[-] YNRSQA(1)[-] LPPTSN(1)[-] 8 Phage display (common panning) 5 PMID:7499368 16-formylgitoxin Anti-digoxin antibody 26-10 Fab Not determined. Not determined. X6 phage display library ELISA The designation + or - refers to whether the clone binds to 16-formylgitoxin used in selection. 173 SHSYIN(6)[+][5.6 ± 1.7] TRYWFN(2)[+][3.9 ± 0.6] 2 Phage display (common panning) 6 PMID:7499368 16-formylgitoxin Anti-digoxin antibody 26-10 Fab Not determined. Not determined. X6 phage display library ELISA,Saturation equilibrium assay Secreted Fab was tested using an ELISA with goat anti-mouse Fab, digoxin-BSA, and the congener-BSA used for selection as coating antigens. All clones were positive for Fab. Besides the designation + or - refers to whether the clone binds to the congener used in selection. Affinities (Ka,e-9 M-1) for digoxin of Fab 26 -10 and mutants (randomly mutated at positions H30 -35 inclusive) selected from the bacteriophage library. Affinities were measured using an equilibrium saturation method with filtration through glass fiber filters for separation of bound and free ligand, except that Fab was immobilized on the filter using goat anti-mouse Fab. 174 IRKTR(3) TRKRR(2) PRPKR(1) IRRPR(1) PRTRR(1) PRPRR(1) YRLPR(1) KRLPR(1) KRTPR(1) RRTPR(1) GRQPR(1) GRIKR(1) PRLRR(1) KRTSR(1) SRHIR(1) VRVAR(1) KRTTR(1) TRIKR(1) RKKRD(1) RNRRS(1) RIKRW(1) KGNLE(1) TSPPL(1) TVRSR(1) KLENR(1) IGDDL(1) CTFTT(1) R-x(2)-R 27 Phage display (common panning) 6 PMID:7987214 Furin Not determined. Not determined. Not determined. AA-xxxxx-AA phage display library 175 LRRFRRP(5) LERVKRY(1) LVRRKRL(1) LPRGKRR(1) PRRFKRP(1) LVRSRRW(1) SRRFRRP(1) DRRFRRP(1) WFRPRRS(1) PRRPRRG(1) IGRPRRS(1) SRRYRRP(1) [LP]-R(2)-F-[KR]-R-P 13 Phage display (common panning) 5 PMID:7987214 Furin Not determined. Not determined. Not determined. AA-xxRx(K/R)Rx-AA phage display library