1576 CEGFLSAWC CFALFGEEC CFDYGYFWC CFLGAWLGC CGCRGDCVC CGFFLFGEC CGLSLDQWC CGLWAGLFC CGLWIGLWC CHMGFFGEC CLLAAWLGC CLLAGWIPC CLLEAWLGC CMLAGWIPC CMLGEWLGC CPLWAALWC CPLWVGLWC CRGPLRLNC CWALFGESC CWASLFGEC CWGLFGERC CWLAPWLGC CWLSEWLGC 23 Phage display (common panning) 3 PMID:18583538 Human adipose stem cell SPARC Not determined. Not determined. CX7C phage display library Authors demonstrate that both primary and cultured human and mouse stromal cells express a conserved receptor targeted by peptides found to mimic SPARC, a matricellular protein that is required for normal WAT development. A signaling receptor for SPARC has not as yet been determined. By using the SPARC-mimicking peptides CMLAGWIPC and CWLGEWLGC, isolated by panning on human and mouse cells, respectively, we identified the α5β1 integrin complex as a candidate receptor for SPARC. 1577 CTEATVAGC CSRGTVWPC CVGGDSTNC CLMSVGVMC CAHSGVLLC CKGPGSGFC CVDVRSGGC CIVGGVLRC CTRVENGWC CDYSSHGFC CTEHGISGC CLLSVFGAC CRVTLRAAC CARVKSREC CLEAGYSVC CRWGAGRTC CDSSLDWHC CAGHQLIVC CPFLRTLRC CEIALCGPC CSDLVRLGC CWLGEWLGC CETRNVASC CSEWSGSLC CAGALGGSC CFGESRNAC 26 Phage display (common panning) 3 PMID:18583538 Mouse 3T3-L1 cells SPARC Not determined. Not determined. CX7C phage display library Authors demonstrate that both primary and cultured human and mouse stromal cells express a conserved receptor targeted by peptides found to mimic SPARC, a matricellular protein that is required for normal WAT development. A signaling receptor for SPARC has not as yet been determined. By using the SPARC-mimicking peptides CMLAGWIPC and CWLGEWLGC, isolated by panning on human and mouse cells, respectively, we identified the α5β1 integrin complex as a candidate receptor for SPARC. 1578 TPPHRHTHHSTL(1) KPPHSHKHPLLT(1) RYQPHPSKTSTS(1) HIMPHLIPVSVL(1) RTQSQPNRHRPR(1) 5 Phage display (common panning) 9 DOI:10.1021/jp809370z Single-walled carbon nanotubes, SWNTs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Single-walled carbon nanotubes (SWNTs) were grown on Quartz. BSA, TBST 0.1, TBST, and Gly-HCl were used as blocking, incubation, wash, and elution buffer solutions respectively. 1579 APAHLHKPSHVR(1) HGNLHKTHLKLP(1) KQPNTHHVHPHS(1) SPKWHPHHQHWR(1) HLRTHPSHHNVP(1) EDPNLQSSLRMP(1) 6 Phage display (common panning) 9 DOI:10.1021/jp809370z Quartz Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) BSA, TBST 0.1, TBST, and Gly-HCl were used as blocking, incubation, wash, and elution buffer solutions respectively. 1580 HLTPTSTWSNPH(2) APHLQHGHHPHR(1) HPPHHQTHHRTP(1) VPKAHHHLHYEA(1) SLSDYHRSPQLS(1) NPGNYTQYRTTN(1) 6 Phage display (common panning) 9 DOI:10.1021/jp809370z Single-walled carbon nanotubes, SWNTs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Single-walled carbon nanotubes (SWNTs) were grown on Quartz. Blocking buffers were not used in whole experimental procedure. TBST 0.1, water, and Gly-HCl were used as incubation, wash, and elution buffer solutions respectively. 1581 PRPALSTGPGRF(2) RPLYDSYNTGMR(1) RP 2 Phage display (common panning) 9 DOI:10.1021/jp809370z Single-walled carbon nanotubes, SWNTs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Single-walled carbon nanotubes (SWNTs) were grown on SiO2 wafer. Blocking buffers were not used in whole experimental procedure. TBST 0.1, TBST, and Gly-HCl were used as incubation, wash, and elution buffer solutions respectively. 1582 PRPALSTGPGRF(1) PSNKRRKDLANV(1) RP 2 Phage display (common panning) 9 DOI:10.1021/jp809370z SiO2 wafer Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Blocking buffers were not used in whole experimental procedure. TBST 0.1, TBST, and Gly-HCl were used as incubation, wash, and elution buffer solutions respectively. 1583 KPPLHNHHHSLP(6) KPPHHHNHPLTK(2) GPPHYHKHKLSA(1) LPHHGHTHKMRV(1) HKLQHLPPPHLR(4) HPPKKPIMNTML(2) HPKPQHAHLKPV(1) HGTKPPHLHSVR(1) HKQHHSPQNFSL(1) KPPDRHVHKLPI(1) 10 Phage display (common panning) 9 DOI:10.1021/jp809370z Single-walled carbon nanotubes, SWNTs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Single-walled carbon nanotubes (SWNTs) were grown on SiO2 wafer. Blocking buffers were not used in whole experimental procedure. TBS, TBS, and Gly-HCl were used as incubation, wash, and elution buffer solutions respectively. 1584 KPTHLHHHTRIL(3) KPLHVHRHHVMD(1) KPVHPHQHLKLS(1) KPIHHHPHLPLK(1) KPPHSHKHPLLT(1) IPPHPHAHHQKR(1) APKNHVHHVHKS(1) HLGPKHPPKHHH(1) 8 Phage display (common panning) 9 DOI:10.1021/jp809370z SiO2 wafer Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Blocking buffers were not used in whole experimental procedure. TBS, TBS, and Gly-HCl were used as incubation, wash, and elution buffer solutions respectively. 1585 DDWSHWWRAWNG(3) YTSPWWLAWYDP(2) AWWEAFIPNSIT(1) WFPIAWPESWYH(1) GWDWAQDWNWWT(1) NDNPWLMWLKNW(1) YEYPWANWWLSP(1) SSAWWSYWPPVA(6) S-x-W(2)-x(2)-W 8 Phage display (common panning) 9 DOI:10.1021/jp809370z Single-walled carbon nanotubes, SWNTs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Single-walled carbon nanotubes (SWNTs) were grown on SiO2 wafer. Blocking buffers were not used in whole experimental procedure. TBS, TBS, and TBST 0.5 were used as incubation, wash, and elution buffer solutions respectively. 1586 HNKHLPSTQPLA SVSVGMKPSPRP VISNHRESSRPL KSLSRHDHIHHH 4 Phage display (common panning) 5 DOI:10.1021/nl049825n L1(0) phase of FePt, L1(0) FePt Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The sequences contain numerous amines, known to be excellent ligands for Pt, lysine, believed to be essential in the mechanism of the binding of HMG domain proteins to the Pt/DNA complex formed in cisplatin-based cancer therapies, and histidine, which is used extensively to bind Pt salts for studying protein activity and for the heavy metal staining of proteins to facilitate in X-ray crystallography. Basic local alignment search tool (BLAST) searches performed on these peptides showed that they all possess similarities to motifs found in naturally occurring proteins. 1587 YGAFQG(2) YGGFLT(2) YGYWSL(1) YGAFMQ(2) YGAFFQ(1) YGAFFK(2) YGFWSN(1) YGAFGG(1) YGGFGF(1) YGVFSR(1) YGGLSM(2) YGTFLN(2) YGGLVR(1) YGSFSL(2) YGAWYT(2) YGRFFH(1) YGGLRH(1) YGSFMA(1) YGGFSP(1) 19 Phage display (common panning) 2 PMID:1443536 Anti-beta-endorphin monoclonal antibody 3-E7 Fab Beta-endorphin Not determined. Not determined. X6 fAFF1 phage display library Phage (2e9 infectious particles in 100 μ1 of TBS) were added to 100 μ1 of TBS containing biotinylated 3E7 Fab (50 pM final concentration) and incubated overnight at 4°C. The mixture was then exposed to streptavidin-coated plates for 3 h at room temperature and bound phage were isolated after 60 min of washing. 1588 CPTHYISTSLC(3)[2.201] CPTHYTTSAPC(1)[2.554] CPTHYISSRHC(1)[2.369] CPSHYVPRIYC(1)[1.993] CPAHYANYLPC(1)[2.234] CAPSLPAGYLC(1)[NT] CPASSHTILVC(1)[NT] CGKFPGSKPSC(1)[NT] CLPAHGPSLSC(1)[NT] 9 Phage display (common panning) PMID:8666827 Anti-HCV polyclonal antibody Genome polyprotein Not determined. Not determined. pVIII-9aa.Cys phage display library (CX9C) ELISA ELISA measurement of reactivity against the indicated phagotopes of Abs affinity purified from serum C12 using phagotope P621c (displaying CPAHYANYLPC) was done. Average values (OD405 nm to OD620 nm) from two independent experiments were determined, reproduced from the graph and shown. The absorbance of the wild type phage was 0.033. NT denotes not tested. 1589 NKTKQNPNL(1)[0.999] KLRRSTNWG(1)[1.729] KSMRSANKP(1)[1.656] NRRNFTAPV(1)[NT] 4 Phage display (common panning) PMID:8666827 Anti-HCV polyclonal antibody Genome polyprotein Not determined. Not determined. pVIII-9aa and pVIII-9aa.Cys phage display library pool ELISA ELISA measurement of reactivity against the indicated phagotopes of Abs affinity purified from serum C12 using phagotope P787 (displaying KLRRSTNWG) was done. Average values (OD405 nm to OD620 nm) from two independent experiments were determined, reproduced from the graph and shown. The absorbance of the wild type phage was 0.078. NT denotes not tested. 1590 MLPKPSSFPVPG(1) LSSVNSFPVVTP(1) HLQIQPWYPQIS(1) VPWMEPAYQRFL(1) QPWLEQAYYSTF(1) SALLPWPVLVNY(1) ITTPWDEMRSFL(1) HSFLHPWDLFDY(1) 8 Phage display (common panning) 3-5 PMID:11520599 Human neuroblastoma cell line WAC 2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Phages associated with WAC 2 cells were recovered in two steps. Cell surface-bound phages were detached by standard acid elution buffer (pH 2.2) for 10 min. Microscopic inspection revealed that this treatment appeared to leave the cells largely intact. In the second step, the cells were collected by centrifugation and lysed by the addition of 1% Triton X-100 in order to recover phages more strongly associated with the cells. Peptides (HLQIQPWYPQIS and VPWMEPAYQRFL) could bind to certain tumor cell types. Thus, these phages and their displayed peptides have the potential to deliver therapeutic agents to the tumors. 1591 GVSKRGLQCHDFISCSGVPW(4) 1 Phage display (common panning) 5 PMID:14500347 CD19+ cells from patient #16 Not determined. Not determined. Not determined. ON543 phage display library (X20) The cell were incubated for 1 h with the phage at 4°C. Cell surface phage were recovered by two acid elutions. The acid fraction was neutralized. 1592 NQSIPKVAGDSKVFCWWCAL(9) DMSFQLVTPFLKALPTGWRG(1) 2 Phage display (common panning) 5 PMID:14500347 CD19+ cells from patient #16 Not determined. Not determined. Not determined. ON543 phage display library (X20) The cell were incubated for 1 h with the phage at 4°C. The tightly associated phage or internalized phage were recovered by hypotonic lysis of the cells in 30 mM Tris (pH 8) on ice for 30 min combined with brief vortexing. 1593 NQSIPKVAGDSKVFCWWCAL(3) QIMMGPSLGYYMPSESIFAY(1) 2 Phage display (common panning) 5 PMID:14500347 CD19+ cells from patient #16 Not determined. Not determined. Not determined. ON543 phage display library (X20) The cell were incubated for 1 h with the phage at 25°C. Cell surface phage were recovered by two acid elutions. The acid fraction was neutralized. 1594 DMSFQLVTPFLKALPTGWRG(4) GGHGRVLWPDGWFSLVGISP(4) QIMMGPSLGYYMPSESIFAY(2) 3 Phage display (common panning) 5 PMID:14500347 CD19+ cells from patient #16 Not determined. Not determined. Not determined. ON543 phage display library (X20) The cell were incubated for 1 h with the phage at 25°C. The tightly associated phage or internalized phage were recovered by hypotonic lysis of the cells in 30 mM Tris (pH 8) on ice for 30 min combined with brief vortexing. 1595 NQSIPKVAGDSKVFCWWCAL(9) QSTPPTKHLTIPRHLRNTLI(1) 2 Phage display (common panning) 5 PMID:14500347 CD19+ cells from patient #16 Not determined. Not determined. Not determined. ON543 phage display library (X20) The cell were incubated for 1 h with the phage at 37°C. Cell surface phage were recovered by two acid elutions. The acid fraction was neutralized. 1596 NQSIPKVAGDSKVFCWWCAL(1) QIMMGPSLGYYMPSESIFAY(9) 2 Phage display (common panning) 5 PMID:14500347 CD19+ cells from patient #16 Not determined. Not determined. Not determined. ON543 phage display library (X20) The cell were incubated for 1 h with the phage at 37°C. The tightly associated phage or internalized phage were recovered by hypotonic lysis of the cells in 30 mM Tris (pH 8) on ice for 30 min combined with brief vortexing. 1597 KPWMTWQWVVEKSSTEGFRT(10) 1 Phage display (common panning) 5 PMID:14500347 CD19+ cells from patient #25 Not determined. Not determined. Not determined. ON543 phage display library (X20) The cell were incubated for 1 h with the phage at 4°C. Cell surface phage were recovered by two acid elutions. The acid fraction was neutralized. 1598 GDELGWNWVTIWPKTLSSRV(6) 1 Phage display (common panning) 5 PMID:14500347 CD19+ cells from patient #25 Not determined. Not determined. Not determined. ON543 phage display library (X20) The cell were incubated for 1 h with the phage at 4°C. The tightly associated phage or internalized phage were recovered by hypotonic lysis of the cells in 30 mM Tris (pH 8) on ice for 30 min combined with brief vortexing. 1599 MTARPDPMMSTSTTVNTVLI(5) 1 Phage display (common panning) 5 PMID:14500347 CD19+ cells from patient #25 Not determined. Not determined. Not determined. ON543 phage display library (X20) The cell were incubated for 1 h with the phage at 37°C. Cell surface phage were recovered by two acid elutions. The acid fraction was neutralized. 1600 LTSPPKEARPSTTVGKSGRE(3) MGTRWQGDGESQHASVGSGS(2) 2 Phage display (common panning) 5 PMID:14500347 CD19+ cells from patient #25 Not determined. Not determined. Not determined. ON543 phage display library (X20) The cell were incubated for 1 h with the phage at 37°C. The tightly associated phage or internalized phage were recovered by hypotonic lysis of the cells in 30 mM Tris (pH 8) on ice for 30 min combined with brief vortexing.