126 ANL 22 phage display library 20 1e10 Brian K. Kay Completely random Linear The ANL 22 library displayed X20 peptides as N-terminal fusions to the protein product of gene III of bacteriophage M13 using SAM vector. 127 Cys6 phage display library 6 2.2e7 Inger Sandlie Completely random Circular The Cys6 library was based on the vector fUSE5, which supports phage fd protein III (pIII) peptide expression. 128 Cys9 phage display library 9 5e7 Inger Sandlie Completely random Circular The Cys9 library was based on the vector fUSE5, which supports phage fd protein III (pIII) peptide expression. 129 NNK11 phage display library 11 9e8 Inger Sandlie Completely random NNK Linear The NNK11 library was based on the vector fUSE5, which supports phage fd protein III (pIII) peptide expression. 130 Evo1 phage display library 6 3e7 Inger Sandlie Semi-random Circular The Evo1 library was based on the vector fUSE5, which supports phage fd protein III (pIII) peptide expression. The insert sequence is XXXCPWFQWPCXXX, where X denotes any residue. 131 Evo2 phage display library 6 3e7 Inger Sandlie Completely random Linear The Evo2 library was based on the vector fUSE5, which supports phage fd protein III (pIII) peptide expression. The insert sequence is XXXXXXWFQWPC, where X denotes any residue. 132 Evo3 phage display library 6 3e7 Inger Sandlie Completely random Linear The Evo3 library was based on the vector fUSE5, which supports phage fd protein III (pIII) peptide expression. The insert sequence is CPWFQWPCXXXXXX, where X denotes any residue. 133 CPEP-8 phage display library (X3CX8CX3) 14 2e9 John Lund Semi-random NNS Circular In the CPEP-8 library, peptides are displayed at the N-terminal of the pIII mature protein on the M13 bacteriophage surface. 134 fdMED1-based X6 phage display library 6 2e8 2e9 Completely random Linear 135 Y-X10-M phage display library 10 Ichio Shimada Completely random Linear This library displayed foreign peptides on the N terminus of the major coat protein (gVIII protein) of bacteriophage M13. Each foreign peptide has a 10-mer random sequence flanked by a Y residue (detectable residue at 280 nm by high-performance liquid chromatography) and a M residue (cyanogen bromide cleavage residue). 136 G-α phage display library (XXLXXXAX) 8 8.4e3 V.A. Petrenko Semi-random NNK Linear G-α-library is a library of phages carrying b-galactosidase-binding peptide ADTFAKSMQ at the N-terminus of the pVIII protein surrounded by random amino acids. 137 fdMED1-based CX6C M13 phage display library 6 5e8 1e12 Mehdi Houimel Completely random Circular 138 X9 T7 phage display library 9 1e8 Roberto Diaz Completely random Linear Random nonamer peptides are displayed on T7 phages as fusion proteins with the amino terminus of 10A capsid protein. 139 CX7-10C T7 phage display library 7-10 Novagen Completely random NNK Circular A mixture of 4 cyclic libraries CXnC was used which displayed 415 copies of the peptide on the capsid. Combined average diversity of the libraries was 1e8. The libraries were based on the T7Select 415-1b vector from Novagen. 140 X7 T7 phage display library 7 1e7 Mark R. Spaller Completely random NNK Linear The library was based on the T7 select 415-1 vector from Novagen. Eahg phage particle can displays 415 X7 peptides. 141 M13LP67-based X12 phage display library 12 2.1e8 Yuzhang Wu Completely random Linear 142 Ph.D.-12 and Ph.D.-C7C phage display library pool 7, 12 New England BioLabs, Beverly, MA, USA Completely random NNK 143 f8-9mer phage display library (X9) 9 2.0e9 V. A. Petrenko Completely random NNK Linear 144 CX10C P99 β-lactamase-fusion library 10 6.1e7 Girja S. Shukla Completely random NNK Circular The library was constructed at the amino-terminus of the Enterobacter cloacae P99 cephalosporinase molecule (P99 β-lactamase) with NNK-scheme of randomization. The β-lactamase enzyme acted as a reporter, saving time and resources otherwise required by the phage-ELISA of a typical phage display screening. 145 X12 P99 β-lactamase-fusion library 12 4.9e7 Girja S. Shukla Completely random Linear The library was constructed at the amino-terminus of the Enterobacter cloacae P99 cephalosporinase molecule (P99 β-lactamase) with NNK-scheme of randomization. The β-lactamase enzyme acted as a reporter, saving time and resources otherwise required by the phage-ELISA of a typical phage display screening. 146 Three pools of fUSE5 phage display library 7-12 Chun-Mei Li Completely random Random peptides were inserted to the minor capsid protein PIII of a filamentous bacteriophage fUSE5. Three pools of such libraries with peptides displayed on the surface of the phage particles were screened. Pool I was composed of four libraries of linear 7- to 11-aa peptides and pool II of three libraries of 9- to 12-aa peptides crosslinked via terminal cysteines, and pool III contained a mix of five libraries with both linear and crosslinked peptides, 8 to 10 aa long. 147 Min-23-R10 phage display library 10 2.8e8 Christelle Souriau Completely random NNK Linear The library was constructed by PCR using a set of degenerate primers to amplify Min-23 and replace residues 17-20 by 10 random residues ( LMRCKQDSDCLAGSVC(X)10FCG ). 148 YLK-(NNK)6 phage display library 6 4.6e7 Fujie Tanaka Completely random NNK Linear The library was appended to the N-terminus of the peptide (YKLLKELLAKLKWLLRKLXXXXXX, X = any of the natural 20 amino acids). 149 (NNK)6-YLK Library 6 4.7e7 Fujie Tanaka Completely random NNK Linear The library was appended to the C-terminus of the peptide (XXXXXXYKLLKELLAKLKWLLRKL, X = any of the natural 20 amino acids). 150 X12 T7 phage display library 12 9e10 Novagen Completely random Linear