1426 CTGPGSWFC(6) CTSPGPWFC(2) CTAPGAWFC(1) CTTPGPWFC(1) 4 Phage display (common panning) 3 PMID:11292756 Anti-E. coli K1 monoclonal antibody HmenB3 Escherichia coli K1 bacteria Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The new mimotopes may be useful in producing a vaccine(s) capable of eliciting anti-NMGB antibodies not reactive with neuronal tissue. 1427 VVSTGSH(5) IPMKGHW(1) LPMKYQA(1) HWGMWSY(1) ALSSDPH(1) DPRLSAL(1) KPSTLML(1) YHWYTSP(1) LFMPATP(1) ALLPWTD(1) SPPPPPI(1) SHAPYTH(1) NQDVPLF(1) 13 Phage display (common panning) 3 PMID:11292756 Anti-E. coli K1 monoclonal antibody HmenB13 Escherichia coli K1 bacteria Not determined. Not determined. Ph.D.-7 phage display library (X7) The new mimotopes may be useful in producing a vaccine(s) capable of eliciting anti-NMGB antibodies not reactive with neuronal tissue. 1428 MELQTRS(5) LPMKYQA(5) NTLQPSP(2) YMTPFSP(2) LHAQSRS(1) YHLQPTP(1) HFMEPVN(1) LSTPSLL(1) ITAARLP(1) SIDNPLP(1) SIITGYL(1) AYSMLGH(1) 12 Phage display (common panning) 3 PMID:11292756 Anti-E. coli K1 monoclonal antibody HmenB14 Escherichia coli K1 bacteria Not determined. Not determined. Ph.D.-7 phage display library (X7) The new mimotopes may be useful in producing a vaccine(s) capable of eliciting anti-NMGB antibodies not reactive with neuronal tissue. 1429 LEKGTPW AAKGTPW EPDKMTT EPDKSII QKTAASY GKQPASD AVTFSKG 7 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #1 Major pollen allergen Bet v 1-A Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Bet v 1. 1430 LEKGTPW AAKGTPW QKTAASY 3 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #1 Major allergen Pru av 1 Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Pru av 1. 1431 LEKGTPW AAKGTPW QVELPKG KLPLTKG QKTAASY EAAKQGE 6 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #1 Stress-induced protein SAM22 Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Gly m 4. 1432 LEKGTPW AAKGTPW EPDKSII EAGKHSG 4 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #1 Ara h 8 allergen Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Ara h 8. 1433 GKIGMYP GKDRSIQ IKLPALF HLVEKGY 4 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #2 Major pollen allergen Bet v 1-A Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Bet v 1. 1434 GKIGMYP FKLKAIE ITADMYT MPNYDRL 4 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #2 Major allergen Pru av 1 Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Pru av 1. 1435 GKIGMYP GKDRSIQ IKLPALF HLVEKGY 4 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #2 Stress-induced protein SAM22 Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Gly m 4. 1436 GKIGMYP IKLPALF 2 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #2 Ara h 8 allergen Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Ara h 8. 1437 KNLEEPK KYLEEPV LNRADIL WPKDTDT 4 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #3 Major pollen allergen Bet v 1-A Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Bet v 1. 1438 KNLEEPK KYLEEPV TPIRHRS QYPWRLA FDMATNP SLYELTH TAIHAVW DHLPSPW 8 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #3 Stress-induced protein SAM22 Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Gly m 4. 1439 YKIGDNW KSDNNVT 2 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #4 Major pollen allergen Bet v 1-A Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Bet v 1. 1440 YKSDNIR SKTDNLT AFNRASD 3 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #4 Stress-induced protein SAM22 Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Gly m 4. 1441 YLMEVQK TLKQAPA 2 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #5 Major pollen allergen Bet v 1-A Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Bet v 1. 1442 YLMEVQK YEMDAPK ERHLTFT 3 Phage display (competitive panning) 2 PMID:16199263 Polyclonal antibody IgE from birch pollen-allergic patient #5 Stress-induced protein SAM22 Not determined. Not determined. Ph.D.-7 phage display library (X7) Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Gly m 4. 1443 GCLYSDLLATCI[1.321] NCLYSDLTQSCI[1.236] NCLYSDLYARCI[1.223] KCMYSDLLGICI[1.153] DCLYSDLESRCI[0.818] SCLYSDLLELCI[0.750] ECMWSDLELRCI[0.571] NCLWSDLEQFCI[0.343] 8 Phage display (common panning) PMID:11413337 Anti-gp120 monoclonal antibody b12 Surface protein gp120 (SU) 2NY7, 1N0X, XCX(3)SDLX(3)CI phage display library ELISA Affinities of selected phages to IgG1 b12 were measured by ELISA. OD405-490, optical density at 405 minus 490 nm, was shown. The absorbances of phage gp120 as positive control and phage f88-4 as negative control were 1.121 and 0.075, respectively. 1444 HERSYMFSDLENRCI[1.236] CSRNQLWSDLHGSCI[1.207] NNQGCLWSDLTASCI[1.189] STTRCTWSDLYDSCI[1.141] QSSSCMWSDLFQQCI[0.992] AQKQCTWSDLLSRCI[0.903] RPCRGVYSDLLDKCI[0.886] SSDHCLWSDLTMTCI[0.644] LPSSCSWSDLLNRCI[0.276] HTCAGTWSDLLSTCI[0.252] 10 Phage display (common panning) PMID:11413337 Anti-gp120 monoclonal antibody b12 Surface protein gp120 (SU) 2NY7, 1N0X, X(7)SDLX(3)CI phage display library ELISA Affinities of selected phages to IgG1 b12 were measured by ELISA. OD405-490, optical density at 405 minus 490 nm, was shown. The absorbances of phage gp120 as positive control and phage f88-4 as negative control were 1.121 and 0.075, respectively. 1445 FNWSLDYLREYH(6)[0.537] ISTVPNYLRHFH(6)[0.536] DKYRIVIARGCV(5)[0.544] LCHPQNCHTKSL(3)[0.547] DPYKLPNRRCCI(3)[0.603] YTIADLITRCCV(2)[0.598] PRDTNTIRRCCV(2)[0.584] PYYNRHHPQDNR(2)[0.556] WGPYPEWLRFYH(2)[0.552] MPDLTTHPQFAR(1)[0.553] RWIDATSHPQFD(1)[0.533] HHPMDNRSYRWP(1)[0.522] GVLHPFHHPGNV(1)[0.530] VVFRPHYLSGFH(1)[0.557] NYHHLRDLRSYH(1)[0.532] HMASWLRFFHGP(1)[0.529] DDFRFTLPRCCV(1)[0.612] DRILAIGYRCCV(1)[0.561] MHVETALVRCCI(1)[0.543] PDRLRSPMRCCI(1)[0.532] DVGFIDRNWCCV(1)[0.360] H-P-[QM], [YW]-L-R-x-[YF]-H, R-C(2)-[VI] 21 Phage display (common panning) 3 PMID:16819727 Streptavidin Biotin 1DF8,1LUQ,1MEP,1MK5,1N43,1N9M,1NDJ,1NQM,1STP,1SWD,1SWE,1SWG,1SWK,1SWN,1SWP,1SWR,1SWT,2F01,2GH7,2IZF,2IZG,2IZH,2IZI,2IZJ,2RTD,2RTE,2RTF,2RTG,2Y3F,3MG5, Not determined. X12 T7 phage display library ELISA After three rounds of biopanning, approximately 1.0e11 PFU of amplified phage pools isolated from each round of screening were analyzed for SA binding in a polyclonal (heterogeneous phage populations) phage ELISA. Absorbances at 650 nm were determined and shown. The absorbance of the phage T7 10-3b as negative control was 0.050. 1446 CHPMRPC(5)[0.654] CHPQFRC(5)[0.638] CHPQVRC(4)[0.630] CHPQGPC(3)[0.677] CVHRFVC(3)[0.639] CVHRFIC(2)[0.668] CHPMVPC(1)[0.613] CPHPMFC(1)[0.582] CHPQFIC(1)[0.676] CHPQFNC(1)[0.728] CHPQFPC(1)[0.774] CHPQNHC(1)[0.707] CHPQNNC(1)[0.667] CHPQNPC(1)[0.751] CHPQVKC(1)[0.597] CHPQVMC(1)[0.602] CHPQYPC(1)[0.632] CKHPQFC(1)[0.639] CLHPQFC(1)[0.681] CRHPQFC(1)[0.666] CIHRFIC(1)[0.696] CIHRFLC(1)[0.662] H-P-[QM], C-[VI]-H-R-F-[VIL]-C 22 Phage display (common panning) 3 PMID:16819727 Streptavidin Biotin 1DF8,1LUQ,1MEP,1MK5,1N43,1N9M,1NDJ,1NQM,1STP,1SWD,1SWE,1SWG,1SWK,1SWN,1SWP,1SWR,1SWT,2F01,2GH7,2IZF,2IZG,2IZH,2IZI,2IZJ,2RTD,2RTE,2RTF,2RTG,2Y3F,3MG5, Not determined. CX5C T7 phage display library ELISA After three rounds of biopanning, approximately 1.0e11 PFU of amplified phage pools isolated from each round of screening were analyzed for SA binding in a polyclonal (heterogeneous phage populations) phage ELISA. Absorbances at 650 nm were determined and shown. The absorbance of the phage T7 10-3b as negative control was 0.060. 1447 CKRHPQVGC(18)[0.703] CVRHPQFGC(6)[0.702] CRKHPQVGC(5)[0.721] CWQVMHLKC(5)[0.623] CQRHPMVGC(4)[0.728] CGHPQFHFC(3)[0.723] CRQMMHITC(3)[0.615] CLPMMHITC(2)[0.515] CHPMAPLRC(2)[0.721] CGHPQRWTC(2)[0.703] CHPQAPRWC(2)[0.731] CGFRHPQFC(1)[0.755] CGHPQIGRC(1)[0.734] CHPQAGKRC(1)[0.757] CHPQFATRC(1)[0.758] CHPQFWRLC(1)[0.817] CHPQNDTGC(1)[0.635] CHPQVYRAC(1)[0.658] CHTKHPQFC(1)[0.708] CLHPQSGRC(1)[0.661] CRKHPQIGC(1)[0.690] CHPMAARNC(1)[0.723] CHPMAPRTC(1)[0.659] CKRHPMVGC(1)[0.676] CKRHPHVGC(1)[0.722] CLHPMNNRC(1)[0.666] CWAVMHIPC(1)[0.543] CWPVMHAFC(1)[0.681] CFVGYGFLC(1)[0.655] CNAMPWFMC(1)[0.605] H-P-[QM], C-x(2)-[VM]-[M]-H-[LIA]-x-C 30 Phage display (common panning) 3 PMID:16819727 Streptavidin Biotin 1DF8,1LUQ,1MEP,1MK5,1N43,1N9M,1NDJ,1NQM,1STP,1SWD,1SWE,1SWG,1SWK,1SWN,1SWP,1SWR,1SWT,2F01,2GH7,2IZF,2IZG,2IZH,2IZI,2IZJ,2RTD,2RTE,2RTF,2RTG,2Y3F,3MG5, Not determined. CX7C T7 phage display library ELISA After three rounds of biopanning, approximately 1.0e11 PFU of amplified phage pools isolated from each round of screening were analyzed for SA binding in a polyclonal (heterogeneous phage populations) phage ELISA. Absorbances at 650 nm were determined and shown. The absorbance of the phage T7 10-3b as negative control was 0.060. 1448 CDPATPYC(43)[0.850] CDLAAPYC(7)[0.752] CDPPTPYC(3)[0.780] CDLSTPYC(2)[0.675] CDVATPWC(2)[0.769] CDWSSPYC(2)[0.812] CDLATPYC(1)[0.809] CDPASPWC(1)[0.877] C-D-P-A-[TS]-P-[YW]-C 8 Phage display (common panning) 3 PMID:16819727 NeutrAvidin Biotin 1AVD,2C4I,2JGS,1DF8, Not determined. CX6C T7 phage display library ELISA After three rounds of biopanning, approximately 1.0e11 PFU of amplified phage pools isolated from each round of screening were analyzed for NA binding in a polyclonal (heterogeneous phage populations) phage ELISA. Absorbances at 650 nm were determined and shown. The absorbance of the phage T7 10-3b as negative control was 0.061. 1449 AFDVRERLL(3) IDWRSRLAP(3) KHDFRSRLA(3) ADWRERLVL(2) AVDWRQRML(2) KTDFRSQLL(2) QDFRWLLT(2) SMDIRNRLL(2) WMDGRHRLL(2) EFRGRLLET(2) ADYRSRLTW(1) AFHVRDRFL(1) AGDFRSRLF(1) ALDWRLRMG(1) ALEFRGRLL(1) DFRGRLFNL(1) DFRNRLTAP(1) DFRLRLLGS(1) DFRSRLLRV(1) DFRLRLVNP(1) DFRYRLLQS(1) DIDFRSRML(1) DLDWRQRLF(1) DTDFRQRLL(1) DWRHRLVNH(1) DWRSRLVIG(1) DWRGRLS(1) EGGYRQRLL(1) EMGFRARLL(1) FDFRSRLLS(1) GADIRARLL(1) GDWRARLAV(1) GIDYRARLL(1) GKDWRGLLL(1) GLDPRARLL(1) GLDWRISLL(1) GPDSRARLL(1) GQDHRERLL(1) GVDFREKLT(1) GWDNRARLL(1) HLDFRARLL(1) HQDWRANLL(1) HSEWRARLA(1) IDWRARLTK(1) ILKDWRHRL(1) IPSFRDRLL(1) IRDFREMLL(1) KDWRGRLVN(1) KLDSRHRLI(1) KMDWRGRLM(1) KQDIRARLL(1) LDFRGSLLV(1) LDIRGRLLG(1) LDIRHRLVN(1) LEDWRGRLL(1) LIDYRQRLA(1) LLDSRARLI(1) MDPRSRLMT(1) MFDFRERML(1) MHDFRARLL(1) MLDRLERLL(1) MLDPRNRLL(1) MNDPRDRLL(1) NDIRGRLLT(1) QADFRDRLL(1) QDFRNRLQL(1) QEDWRGLLL(1) QKDFRNRLA(1) QSSFRERLL(1) RRMLRARLL(1) SDWRGLLL(1) SDWRGRLGA(1) SELFRSRLL(1) SFDWRGKLL(1) SLWDWRGRL(1) SMDLRSRL(1) STDFRQRLF(1) TDDFRSRPL(1) TDFRHRLGM(1) TIEFRHRLL(1) TRDVRERLL(1) VADIRARLL(1) VGDWRHRLM(1) VHGDFRNRL(1) WDFRNRLV(1) WDLRERLI(1) WFPEWRMTS(1) WGDFRANLI(1) WGGFRDRLL(1) YEDWRGRLL(1) 90 Phage display (common panning) 3 PMID:17675514 Anti-FPR monoclonal antibody NFPR1 fMet-Leu-Phe receptor, fMLP receptor Not determined. Not determined. J404 phage display library (X9) 1450 LPSISDLL(28) ETTLPSIKW(13) GALDQSLPS(2) PSISDLLAI(2) RDTLPSIFW(2) RETLPSLYL(2) SQWWRHNDM(2) TLPSLMRHI(1) TTLPSISDLL(1) TTLPSISDWL(1) WWGGTLPSL(2) AATLPSIDW(1) LPSIDAVLN(1) ESIAFWNRRPA(13) DSIAFWNRRPA(12) CLTCWNVMPA(1) EVASYWRWG(9) ETLDWWRVG(4) ETRDWFRFE(3) EVRGWSRIW(1) EVFGWDRFW(1) EVIGWSRLF(1) ETRDWWRLS(1) KHLHRHKIG(1) LCIPGFC(1) 25 Phage display (common panning) 3 PMID:17675514 Anti-FPR monoclonal antibody NFPR2 fMet-Leu-Phe receptor, fMLP receptor Not determined. Not determined. J404 phage display library (X9)