1276 QLETFSIKNMPR TSIPHIMYLDPF FKWPTTEWPSRL NFMESLPRLGMH SLTVPFLPLYVP ASRQDMQNLAPP E-S-L-S-R-x(2)-M-x(2)-L-x-P 6 Phage display (competitive panning) 3/4 PMID:20619902 Anti-IL-2 monoclonal antibody 3A1 Interleukin-15 Not determined. Not determined. Ph.D.-12 phage display library (X12) Bound phages were competitively eluted with recombinant goose IL-2. 1277 WAPGSMPTSRLA HLPTSSLFDTTH SHPWNAQRELSV ATWSHHLSSAGL DRYNSEWLPYSP SSPHHMVSYTWL S-H(2)-L-P-T-S-x-L 6 Phage display (competitive panning) 3/4 PMID:20619902 Anti-IL-2 monoclonal antibody 3B3 Interleukin-15 Not determined. Not determined. Ph.D.-12 phage display library (X12) Bound phages were competitively eluted with recombinant goose IL-2. 1278 VPVIWDVPYQSL MHNPWDAFMEAS GHPDPWLIEAPS SHDPWDLLPLPY MHPDPWDAPESR YHSDPWSGFLKD KPVYWDHELSKS H-P-D-P-W-D-A-P-L-S(2) 7 Phage display (competitive panning) 3/4 PMID:20619902 Anti-IL-2 monoclonal antibody 5A9 Interleukin-15 Not determined. Not determined. Ph.D.-12 phage display library (X12) Bound phages were competitively eluted with recombinant goose IL-2. 1279 TLKPTQASLVHG HVGTGDGHEHWQ QLETFSIKNMPR HSNWRVPSPWQL GIHELNVARLRL H-E-P-W-Q-L-x-L 6 Phage display (competitive panning) 3/4 PMID:20619902 Anti-IL-2 monoclonal antibody 5C3 Interleukin-15 Not determined. Not determined. Ph.D.-12 phage display library (X12) Bound phages were competitively eluted with recombinant goose IL-2. 1280 CFRHMTEQC(6)[0.545] CIPLPFYNC(5)[0.22] CSHLYLHNC(2)[0.464] CPLTGTSKC(1)[1.04] CTLKVRGEC(1)[0.97] CGFWHTSKC(1)[0.411] CAWPSKDNC(1)[0.887] CIPLLFHNC(1)[0.893] CWGAMVHPC(1)[0.452] CHASLKHRC(1)[0.785] CYTPWMPRC(1)[2.01] CTYLAPKGC(1)[0.851] 12 Phage display (common panning) 4 PMID:19027991 Beta-amyloid protein 42 (Beta-APP42) Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) ELISA To determine the apparent dissociation constant (Kd, nM), the amyloid-beta 42 coated wells were incubated with twofold serial dilutions of each phage clone, starting with 1.0e12 virions(1.66e(-8) M) in the first well and ending with 8.0e6 virions (1.33e(-13) M) in the last well. Kd of the 12 selected clones displaying the highest affinities for amyloid-beta 42 was shown. Data shown were reproduced from the graph. Two phage clones expressing the peptides C-IPLPFYN-C and C-FRHMTEQ-C were strongly selected because they are present in several copies. The first peptide is characterized by five hydrophobic amino acids out of seven suggesting that it interacts with Aβ42 by hydrophobic bonds. It is interesting to note that Aβ42 itself aggregates by hydrophobic mechanisms inside AP structures. On the other hand, peptide C-FRHMTEQ-C is hydrophilic, so the interaction mechanism should be differ ent and could involve the hydrophilic N-terminus domain of Aβ42. 1281 TSYTTSIFGPRA(1) ILANDLTAPGPR(1) NYMSALAITTSL(1) KPANLTSTPWVP(1) LLADTTHHRPWT(1) ISMPVRPLLQDF(1) SIVSTQTSLPLN(1) SMTSHNQWHLLA(1) ANPSPSTHHLTP(1) TTTLLTATPHPH(1) YPSFTHSATPSL(1) FHQNSLRVHSSP(1) QDVHLTQQSRYT(1) NLNHERSQNLKM(1) YESIRIGVAPSQ(1) ERVMLPFPPAPW(2) IPWTQHMAMSPM(1) TNTSWMTAMTPF(1) THTTNAEGYSPV(1) TMGFTAPRFPHY(1) SVSVGMKPSPRP(4) SINGQWMRAIGK(1) GIQLANPPRLYG(1) QDMLKPYVDPLH(1) SHHIPSYQWPLH(1) WAETWPLAQRPP(1) SNQTSDRPPLLT(1) SSLEPWHRTTSR(2) EWLAYDGIRAYS(1) ETLPITLIARLT(1) 30 Phage display (subtractive panning) 5 PMID:20501662 Glycine receptor subunit alpha-1 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Human Embryonic Kidney (HEK) Cell were transfected with GlyR α1 subunit cDNA. Phage were applied to blank (control) HEK 293 cells. Then, phage that did not bind in this negative selection procedure were removed from the plate with a pipette, applied to the plate of GlyR-expressing cells. 1282 DHARYPWLRPPA LPAFFVTNQTQD YWNASPSASGVI WHTEILKSYPHE NSPRLVHTNTHN LLADTTHHRPWT SHVDDLGLRPLT 7 Phage display (common panning) 3 PMID:20544195 Anti-EGFR monoclonal antibody 12H23 Epidermal growth factor receptor Not determined. Not determined. Ph.D.-12 phage display library (X12) Genomic DNA from 20 phage clones was sequenced, and seven different insert sequences were observed. Two (WHTEILKSYPHE and LPAFFVTNQTQD) mimotope candidates were specifically recognized by the selecting antibody 12H23 but not by the isotype-matched control antibody. 1283 ASGALSPSRLDT(13) 1 Phage display (subtractive panning) 4 PMID:20570932 Human 143B osteosarcoma cell lines Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Before each selection round, a negative selection with the 293T human embryonic kidney cell line was done to subtract phages that bound to nontumor cells. After the final selection round, the ssDNAs of 20 clones were sequenced and analyzed. One sequence was found to be enriched in 65% of all sequenced clones. Peptide ASGALSPSRLDT shares a significant homology with heparinase II/III family protein, which binds and reacts with heparan sulfate proteoglycans. 1284 SVSVGMKPSPRP(8) NHNTSTWAAYST(1) TLPSPHSLLTVH(1) 3 Phage display (common panning) 3 PMID:20329714 GaAs semi-insulating substrate Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The specific adhesion of these peptides is used for biofunctionalization of GaAs/AlGaAs photonic waveguides capable of enhancing the second harmonic generation response. 1285 YLCKFGC(1) SPDELHK(2) REPLVYW(1) AEPIIYW(2) KQPIVFW(1) 5 Phage display (common panning) 4 PMID:20844088 Anti-SEB monoclonal antibody ab53981 Enterotoxin type B (SEB) Not determined. Not determined. Ph.D.-7 phage display library (X7) The N-terminal 15-mer peptide of SEB was determined to be an epitope of ab53981. 1286 MGGTLIASDQYQ(17) QSLPASMSYQTA(1) SLSASMDFMMYA(1) NALRASNSFMDE(1) L-x(2)-S 4 Phage display (common panning) 4 PMID:20844088 Anti-SEB monoclonal antibody ab53981 Enterotoxin type B (SEB) Not determined. Not determined. Ph.D.-12 phage display library (X12) The N-terminal 15-mer peptide of SEB was determined to be an epitope of ab53981. 1287 AHPWWLG(4) SHPWYLG(2) P-x(2)-L-G 2 Phage display (common panning) 4-6 PMID:20652782 Anti-S100A4 monoclonal antibody 22.3 Protein S100-A4 Not determined. Not determined. Ph.D.-7 phage display library (X7) The consensus sequence of MAb 22.3 mimotope was corresponded to amino acids 43-47 of S100A4 amino acid sequence. 1288 AWPDKQP(1) SWPDKMP(1) ILSDKMA(1) PDKQP 3 Phage display (common panning) 4-6 PMID:20652782 Anti-S100A4 monoclonal antibody 22.1 Protein S100-A4 Not determined. Not determined. Ph.D.-7 phage display library (X7) The consensus sequence of MAb 20.1 was corresponded to amino acid 94-98 of the C-terminal part of S100A4. 1289 HGINTTKPFKSV WPSSYLSPIPYS AVTTLTLVPAGT ALTGSTKSAFGG 4 Phage display (common panning) 6 PMID:20405826 Na+ Montmorillonite (MMT) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 1290 HGINTTKPFKSV TSVRTHFPLYPV GFEHKDQRFTRE 3 Phage display (common panning) 6 PMID:20405826 Primary Ammonium C18 Modified Montmorillonite (MMT) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 1291 HGINTTKPFKSV GTFPLAIKARDP LHLPSDRLRLWG MHRSDLMSAAVR 4 Phage display (common panning) 6 PMID:20405826 Quaternary Ammonium C18 Modified Montmorillonite (MMT) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 1292 LSVTYCFVY NSIWGLFVH PVNMTFVAL RFSTYVSSW VHFSYVSVV RYSCARSTS WYARFNLAA WVRFVGVTG GRWVALLDV SVARFLVVD RVRPARYLG YVQVARYLS RFLGMRRGF VRWLAWKMV LRYIMALAV GTRFALETS WDRWAMSTG RYWVVIETV SKGARFWLR RWGFRFFAG FSRYFSLRL YSRWEVRFM AWVRFWEDD RYLEIADFK LRVSFLDIA FVSLWLEVA AWGVYLDLG WGVYLEAVS YVTGYLDVV GRVFFWDLE MVGFWELVR PPRGWKLEW VSLYSSDGL VVGYLMEVL VSLFMGIDF VYTRLEGCL VVAFLMGQD QGFLSFLRE WAWLMATME WIFYSLSTE WVLPVQWSM VGFLFNLLW YVFRMRLVS VVFLAAGSY WLSLLNVRV MIGYAVRPH FWAFALARA EPYMLGVTR GTCYMSVVS VRMMCWYPV EARVLACLF 51 Phage display (common panning) 5 PMID:20338912 Chymase Not determined. Not determined. Not determined. X9 T7 phage display library Nonapeptide-coding inserts were then sequenced from 75 individually sampled phages. Fifty-one of the obtained sequences were aligned. Seventeen background sequences that had also been observed in analyses of other enzymes were excluded. Eleven of these contained the insert WCQVQSVCA and six the insert TLMVPRTGS. Four sequences contained stop codons. The remaining three sequences contained repetitive sequences and did not contain aromatic aa. 1293 RLQLKL(2) RTRYED(3) RIPLEM(3) QFDEPR(3) TSAVRT(2) QCTGRF(1) ITDMAA(1) WRPCES(1) 4 Phage display (in vivo) PMID:20460372 Breast cancer tumors Not determined. Not determined. Not determined. pComb M13 phage display library 1294 RLQLKL(11) GLWQGP(7) QCTGRF(5) LPGMMG(5) DVGTTE(5) TDLGAM(5) GMMYRS(4) DSNAES(4) ITDMAA(3) RWRTNF(4) WRPCES(2) WRNTIA(3) IDKQLE(3) FMEIET(3) HEVVAG(2) GGHTRQ(2) INGKVT(2) 17 Phage display (subtractive panning) 6 PMID:20460372 Mixed liver/kidney tissue extracts Not determined. Not determined. Not determined. pComb M13 phage display library Phage library was pre-exposed to extracts of liver and kidney, tissues that typically have high nonspecific uptake of drugs and imaging agent, to allow cleavage by proteases contained within these extracts. Isolated phage were reamplified and subjected to six additional rounds of both negative (no liver/kidney cleavage) and positive (tumor cleavage) selection, with representative phage being isolated and sequenced after each round. 1295 QQSWPIS(4) 1 Phage display (common panning) 3 PMID:20648291 Pd wire Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Peptide QQSWPIS appeared four times among ten colonies. The peptide can bind to the Pd NC surface and act as a stabilizer to mediate Pd crystal nucleation and growth. 1296 MFQRMAVDA(1) YRQMSAPTL(1) WFGQLQAAQ(1) YRHAQAYGV(1) MPRQYTQMI(1) FHKVYRGLL(1) WQMAMSRHL(1) SIMMMGAVR(1) WYTLLKSRL(1) YKPMLASVG(1) YAAMRGGQI(1) YKPLWGQMT(1) NYQMMGTMR(1) FERARSRLL(1) YLRMLQILK(1) NWRALRGAL(1) YRQMWVNRA(1) FMAMRAVRL(1) FTMLMNQVM(1) VYRAQGPQM(1) MMSRMLGQT(1) SSGRYARLG(1) TFYRMGTQM(1) WIGQMYAAK(1) YRMQSNHVS(1) DRSMEWRMM(1) FSAIRNRIL(1) YRAMQTTLS(1) NYLGMKPRV(1) RGFRHMMAA(1) YQQMGARLM(1) SWRHIQTRV(1) MYGLMLAQR(1) YQALRAYWQ(1) RFTQMMAVA(1) WKGLQGALS(1) FMRLGGGHL(1) IWHMQNARV(1) YKLLRATQM(1) LWQSFMQMA(1) FHQLRGGSL(1) YRGMQRRTL(1) YGKLRGGTL(1) QVGKYLGLG(1) TYRGMAGFR(1) RVFQNLRAS(1) WRQMYRAQL(1) RSYSLAPGR(1) TWNSLRGRL(1) QRYNAMRAA(1) YIMQMNRVV(1) LTLMQAKRL(1) RFTQMGALA(1) YYGKMPALV(1) PWQAMRGWL(1) KKIMSYHAL(1) YRGMSAFRA(1) MFHRMSGLQ(1) GQRSYSSMQ(1) YMMQKSVRA(1) 60 Phage display (common panning) 4 PMID:1402647 HLA class II histocompatibility antigen, DRB1-1 beta chain HLA class II histocompatibility antigen, DM Not determined. Not determined. M13lib X9 phage display library DR1 molecules were isolated from the human EBV-B cell line, HOM-2. M13 phages were incubated with biotinylated DR1 in binding buffer (50 mM Tris-C1, pH 7.5, 150 mM NaC1, 1 mM EDTA, 1 mM PMSF, and 0.2% NP-40). After at least 24 h incubation at room temperature, BSA-blocked streptavidin on 4% beaded agarose was added and incubated for 10 min. The M13 phage/DR1 complexes were purified by washing the solid phase several times with binding buffer. Competition experiments with both isolated phages and corresponding synthetic peptides showed that the binding was specific. 1297 EPLQLKM QQQLSTH 2 Phage display (common panning) 5 PMID:20942387 TIMREX SLP30 Primary Synthetic Potato graphite Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Modification of graphene with bifunctional peptides reveals both the ability to impart selective recognition of gold nanoparticles and the development of an ultrasensitive graphene-based TNT sensor. 1298 TMGFTAPRFPHY YHRMPQALSAME 2 Phage display (common panning) 5 PMID:20942387 TIMREX SLP30 Primary Synthetic Potato graphite Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Modification of graphene with bifunctional peptides reveals both the ability to impart selective recognition of gold nanoparticles and the development of an ultrasensitive graphene-based TNT sensor. 1299 GAMHLPWHMGTL 1 Phage display (common panning) 5 PMID:20942387 AFM standard highly ordered pyrolytic graphite (HOPG) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Modification of graphene with bifunctional peptides reveals both the ability to impart selective recognition of gold nanoparticles and the development of an ultrasensitive graphene-based TNT sensor. 1300 GAMHLPWHMGTL 1 Phage display (common panning) 5 PMID:20942387 N006 nano graphene platelets Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Modification of graphene with bifunctional peptides reveals both the ability to impart selective recognition of gold nanoparticles and the development of an ultrasensitive graphene-based TNT sensor.