1251 AIWPEPLPLPIG(5) GSNGIWFNLAHR(3) MDSRLGLWPLTW(1) TGLWPSQAQNKA(1) GIWPE 4 Phage display (common panning) 3 PMID:20810747 Anti-E(rns) glycoprotein monoclonal antibody M2172 E(rns) glycoprotein Not determined. Not determined. Ph.D.-12 phage display library (X12) 1252 QNWSSAKLPWAP(2) ETWSSVKLPPGI(2) TWQSAKLPWTRP(1) YGWTSGRLPNPP(1) STWPAFRLFTNI(1) YTTTSFRLPNVS(1) YQLRPNAESLRF(1) THSWTSGKIPLR(1) T-x(2)-K-L 8 Phage display (common panning) 3 PMID:20810747 Anti-E(rns) glycoprotein monoclonal antibody M2171 E(rns) glycoprotein Not determined. Not determined. Ph.D.-12 phage display library (X12) 1253 TPVALQAKNSAP(1) AVTTQARNIPTV(1) HPLPQARNLPTI(4) TSQQAKNTPTHT(1) LQPQAKNVPTSS(1) EVVYQQSPNTPT(1) V-x(2)-Q-A-R-N-x-P-T 6 Phage display (common panning) 3 PMID:20810747 Anti-E(rns) glycoprotein monoclonal antibody M2165 E(rns) glycoprotein Not determined. Not determined. Ph.D.-12 phage display library (X12) 1254 LPYSQSGTLVPP(2) SPLLMSLGGSIV(1) FQYSKAGSFVPE(1) NWSKHGTLLPLV(6) S-x(2)-G-T 4 Phage display (common panning) 3 PMID:20810747 Anti-E(rns) glycoprotein monoclonal antibody M2180 E(rns) glycoprotein Not determined. Not determined. Ph.D.-12 phage display library (X12) 1255 APLLVNQSLPHR(1) LTGGTGYSHDFR(2) WAPGSMPTSRLA(2) TTDKHSMTPAAS(1) SHPWNAQRELSV(1) LPFVEWSGISYF(1) QDVHLTQQSRYT(1) TPSLPPTMFRLT(1) L-V-x-G-S-M-P-S 8 Phage display (competitive panning) 3/4 PMID:20452371 Anti-IL-2 monoclonal antibody 1H4 Interleukin-2 (IL-2) Not determined. Not determined. Ph.D.-12 phage display library (X12) Bound phages were competitively eluted from the well with recombinant duck IL-2. Predictive native epitopes L13I14K15G21S23M24P34S41 were determined through aligning the consensus mimotope motifs with duck IL-2 by Clustal W. 1256 KPHTPHNHPSHH(1) KPHHKDIPHSAM(2) KPISHHPHHRAW(1) GPHKHKMTHEHV(4) APHKHHKPPVLM(2) K-P-H-K-H(2)-x-H(2)-S-H-M 5 Phage display (competitive panning) 3/4 PMID:20452371 Anti-IL-2 monoclonal antibody 2B3 Interleukin-2 (IL-2) Not determined. Not determined. Ph.D.-12 phage display library (X12) Bound phages were competitively eluted from the well with recombinant duck IL-2. Predictive native epitopes T33P34T37K38E39C40S41W42Q43T44Y48L49 were determined through aligning the consensus mimotope motifs with duck IL-2 by Clustal W. 1257 THLDKWPKAKPD(3) WPHHKAMPPKIK(5) HSSWWLALAKPT(2) W-x(3)-K-A-K-P 3 Phage display (competitive panning) 3/4 PMID:20452371 Anti-IL-2 monoclonal antibody 5F6 Interleukin-2 (IL-2) Not determined. Not determined. Ph.D.-12 phage display library (X12) Bound phages were competitively eluted from the well with recombinant duck IL-2. Predictive native epitopes W42Q43D67E68K69V70K82P88 were determined through aligning the consensus mimotope motifs with duck IL-2 by Clustal W. 1258 YQDILPNERTQL(2) HDIIERVLPKMP(3) HVLNERYPTSLN(3) IPMEHYPLRKHG(1) VPWELYSLRNRP(1) HVPNERYPLR 5 Phage display (competitive panning) 3/4 PMID:20452371 Anti-IL-2 monoclonal antibody 4G12 Interleukin-2 (IL-2) Not determined. Not determined. Ph.D.-12 phage display library (X12) Bound phages were competitively eluted from the well with recombinant duck IL-2. Predictive native epitopes Y32T33P34N35E58R74F103P104L114R115 were determined through aligning the consensus mimotope motifs with duck IL-2 by Clustal W. 1259 KTVHIGP KTLHIGP CQGKLTC CHGKLTC HQASNFK HLASNYK 6 Phage display (subtractive panning) 3 PMID:20637241 Anti-HIV1 plasma IgG Envelope glycoprotein gp160 Not determined. Not determined. Ph.D.-7 phage display library (X7) Plasma IgG was linked to magnetic microbeads coated with an anti-human IgG. Three rounds of positive selection were performed. The negative selections and amplifications followed the first and second rounds of positive selection. After the third positive selection, individual colonies were picked at random and subjected to analysis by phage capture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hage display (subtractive panning) 3 PMID:20637241 Anti-HIV1 plasma IgG Envelope glycoprotein gp160 Not determined. Not determined. Ph.D.-12 phage display library (X12) Plasma IgG was linked to magnetic microbeads coated with an anti-human IgG. Three rounds of positive selection were performed. The negative selections and amplifications followed the first and second rounds of positive selection. After the third positive selection, individual colonies were picked at random and subjected to analysis by phage capture ELISA. 1261 WLSEAGPVVTVRALRGTGSW 1 Phage display (common panning) 6 PMID:15313615 Adherent primary cardiac myocytes, APCM Not determined. Not determined. Not determined. X20 fAFF1 phage display library The isolated phage, displays the peptide WLSEAGPVVTVRALRGTGSW, and binds these cells 180 times better than a control phage from the library. Furthermore, phage displaying this peptide preferentially bind to cardiomyocytes when compared with a panel of other cell types. A BLAST search revealed that this peptide contains a 12 amino acid segment with sequence identity to a peptide in tenascin-X, an extracellular matrix protein. 1262 GTYNLPNPPPPL(3) KHMHWHPPALNT(3) SAHGTSTGVPWP(3) VPTATLMGASAR(2) WAETWPLAQRPP(2) LSTHTTESRSMV(2) SGHQLLLNKMPN(2) THAAHMGYPSWW(1) LLADTTHHRPWT(2) 9 Phage display (subtractive panning) 4 PMID:20713104 Mouse embryonic stem (ES) cells Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) To avoid non-specific binding peptides, differentiated mouse ES cells were used as negative target. After four rounds of negative-positive selection, nine sequences in 20 random samples from the chosen clones were selected. Enzyme-linked immunosorbent assay results suggested the peptide (KHMHWHPPALNT) had high affinity and specificity to the mouse ES cells. Immunofluorescence analysis confirmed that peptide (KHMHWHPPALNT) phage selectively bound to the mouse ES cells but not to the differentiated mouse ES cells. 1263 WKVRKSFFKLQG(1) WKLRKWFFKDGG(1) WKAQKRFMKKSG(1) WKVRKAFLFAKN(1) WKMRKSFHKVPG(1) GRKSFHKLDGSF(1) LKTRKLFWKYKD(1) PTTRKWWLKLDG(1) 8 Phage display (common panning) 3 PMID:20833181 Anti-LBP monoclonal antibody M392-2 Lipopolysaccharide-binding protein (LBP) Not determined. Not determined. Ph.D.-12 phage display library (X12) Eight phage clones were found to significantly inhibit the sensitization of LBP on LPS-induced TNF-α release in PBMC. These eight clones had no inhibitory activities on the reinforcement of LPS internalization by LBP, and their displayed peptides indicated the ability to inhibit the inflammatory effects of LBP. 1264 CLHQHNQMC(10) CSRAELSHC(5) CHKSAPTAC(5) CNTSEPRQC(4) CQSDSLSTC(4) CSPTSNSMC(4) 6 Phage display (in vivo) 3 PMID:20863866 Rat lactating mammary gland Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Total 249 peptide sequences were determined from randomly selected individual phage recombinants rescued from lactating mammary tissue after the third round of biopanning. 6 representative peptide sequences were chosen based on their superiority of the appearing frequencies for next study. A peptide ligand, CLHQHNQMC, which specifically homes to the mammary tissue during lactation, was identified through the consecutive in vivo biopannings. The peptide ligand showed specific binding affinity to lactating mammary tissue without any preference to other organs tested in ex vivo and in vivo validation, and microscopy analysis revealed that systemically introduced MG1 could be specifically localized in the lactating mammary gland associated with mammary epithelia and alveolar vasculature. 1265 RNLWPGDLRWVGWHL(1) LGHIWTGRFYAPYRT(1) AREYGTRFSLIGGYR(1) RLGPLHFLNAWGHDH(1) PFYRAGLHSRLGLGG(1) HSAIYYKNFGSSLFR(1) SNLRSWLFPFDRVGN(1) VTGHRWSVRQLGLSH(1) 8 Phage display (common panning) 4 PMID:20609525 Rhipicephalus microplus eggs Not determined. Not determined. Not determined. f88-15mer phage display library (X15) Fourteen individual clones were randomly picked from the eluate of the fourth round of affinity selection and independently propagated for phage production. Eight of the 14 phage clones showed binding on the dot-blots, whereas the remaining six clones no evidence of binding. 1266 CLNSFLRSC CLSTALRSC CSSWYRGAC CTGTSTRAC 4 Phage display (subtractive panning) 4 PMID:20434854 Intact oocytes surrounded by ZP proteins Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) To remove phage clones that bind to the plastic material of test tubes, the primary library was added to an empty test tube prior to incubation with oocytes. Thirty-two random phage clones were analyzed after the fourth round of selection and each clone was sequenced for phage DNA. Sequencing led to identification of three dominant groups of peptide sequences [CLNSFLRSC, CVLSTALRSC (37.5%); CSSWYRGAC (22%); CTGTSTRAC (9%)]. An additional group of peptides represented by only one sequence each (most likely background clones) was isolated as well. 1267 AHPNTAPIHPKF(2) NNQSPILKLSIH(5) WQPVNNAGAILM(1) ATPQNNMMQAQW(1) N(2)-x(2)-P-I-L 4 Phage display (subtractive panning) 4 PMID:20434854 Intact oocytes surrounded by ZP proteins Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) To remove phage clones that bind to the plastic material of test tubes, the primary library was added to an empty test tube prior to incubation with oocytes. NNQSPILKLSIH peptide stimulated production of anti-peptide antibodies. These antibodies bind to the acrosomal region of the canine sperm cell, demonstrating ability to act as sperm antibodies. 1268 DAWKMRLSQMYD VNYNGKEHLLIP IDPLMFKYWSNM SPLNNFYKTQLR VNWNSWHKTNLS AGLPNHNAMLLT NAWLQEPNHRNL TLFTPDKSPAKT SNNADYKQSLLL GALHQEPSSNLF GLFTPDKSPAKT APYDTPWPSPSL CLHCKYTLQQQA GALIYTPEKYTI IDPLMFKYWYNM GPYDTPMFSLNM HAWQSKTPDKTR HTQHSPMVSVEF HVRIPPTMPEAW IDSNHVYKDFLT IPYTHAHADHTL SSKLTFIDFHRN SHDGASSKIRPA SIPTYTPDKVTY SQKYFNDLLDHQ KPVVGMPIVEVW SVPLAKRHLISL SWSSAERLYTMN SYGSSVTQHLAT 29 Phage display (common panning) 3 PMID:20488622 Anti-larval proteins of R. microplus polyclonal antibodies IgG Larval proteins of Rhipicephalus microplus Not determined. Not determined. Ph.D.-12 phage display library (X12) 1269 CTGSWFRPC(1) CLPSWHRFC(2) CLSTGFRAC(1) CHPLRHRPC(1) CVPTWYRAC(1) CLPLTPRSC(1) CSPFNPRVC(1) CTPSWFRWC(1) CSPNWLAHC(1) P-x-W-x-R 9 Phage display (competitive panning) 3 PMID:20594626 Anti-tick subolesin polyclonal antibody (S2) Subolesin Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Sera from non-immunized animals were used as negative control. Phages bound to IgG were sequentially eluted by competition with recombinant tick subolesin ortholog proteins. 1270 CLGPYSFVC(1) CSKLPLALC(1) CNLSRAPFC(1) CLGSYSFIC(1) CSSLDSPMC(1) CYPWGQDHC(1) CPSVLHGWC(1) 7 Phage display (competitive panning) 3 PMID:20594626 Anti-tick subolesin polyclonal antibody (S9) Subolesin Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Sera from non-immunized animals were used as negative control. Phages bound to IgG were sequentially eluted by competition with recombinant tick subolesin ortholog proteins. 1271 CPAAAQALC(1) CPGSFSFIC(1) CGWYSFQHC(1) CGYSFSTSC(1) CWWKPAHLC(2) CPWYSSALC(1) CPAAFAFLC(1) CPSAFHFLC(1) CWYSFSAVC(1) Y-S-F-S-P-x-A-F-S-F-L 9 Phage display (competitive panning) 3 PMID:20594626 Anti-mosquito subolesin polyclonal antibody (S4) Subolesin-like protein Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Sera from non-immunized animals were used as negative control. Phages bound to IgG were sequentially eluted by competition with recombinant mosquito subolesin ortholog proteins. 1272 CPWYLSSLC(1) CISPTDNMC(1) CSLFASAQC(1) CVLPWPWEC(1) CSLNSSAQC(1) CTMPWPFPC(1) CSLPWPFPC(1) CGLGGTAQC(1) CPYGAHWFC(1) S-L-x(2)-S-A-Q, PWP 9 Phage display (competitive panning) 3 PMID:20594626 Anti-mosquito subolesin polyclonal antibody (S5) Subolesin-like protein Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Sera from non-immunized animals were used as negative control. Phages bound to IgG were sequentially eluted by competition with recombinant mosquito subolesin ortholog proteins. 1273 APTPSPGPSYRG(3) SLPTPTPGPWTR(4) YPTPSPGPSARP(2) NTPTGSPGPSTR(1) TPTGQIGPPMRE(1) TPTQSPGPGSRP(1) TPTASFGPTHRS(2) YKPDPTFGPSNR(2) NPDPSPGPTTRH(4) DPPPTFGPHSRS(1) TPPPYWGPHSRD(1) TPSPTMGPPARP(1) QPPHQWGPPSRG(3) LHKPWSPGPSYR(2) QPMPWTAGPTSR(1) IYTPPTWGPPRQ(3) AQPPTPPAGKFR(1) TPTPSPGVLFKT(2) SSVPTPSPGAPF(1) P-T-P-x(2)-G-P-x(2)-R 19 Phage display (common panning) 4 PMID:20630806 Anti-Ap[17-30] polyconal antibody from patient 1 Histone H3-like centromeric protein A Not determined. Not determined. Ph.D.-12 phage display library (X12) 62 phage supernatants of randomly selected bacterial colonies were tested for Ap[17-30] specificity in an ELISA, which identified 36 positive phage clones. 1274 QHSTLPSPGPSL(16) SPTTPSPGPSMK(1) APTPSPGPSYRG(6) AEYRPSPGPSAH(1) NTPSPGPSKAIA(2) FKGQDPSPGPSR(2) TLKPSDGPSRFW(1) DRPSMGPSQFHT(1) STLPSMGPSNFF(1) DANYPSHGPSRY(1) TYLPTKGPSRTV(1) P-[ST]-x-G-P-S 11 Phage display (common panning) 4 PMID:20630806 Anti-Ap[17-30] polyconal antibody from patient 8 Histone H3-like centromeric protein A Not determined. Not determined. Ph.D.-12 phage display library (X12) 62 phage supernatants of randomly selected bacterial colonies were tested for Ap[17-30] specificity in an ELISA, which identified 36 positive phage clones. 1275 GHPDPWLIEAPS HHDPWDRLERFT VHDPWSNNSTWN SHDPWDLLPLPY SHDPWDEGPQRA AHSERWERFPQG H(2)-D-P-W-D-x-LP 6 Phage display (competitive panning) 3/4 PMID:20619902 Anti-IL-2 monoclonal antibody 2E6 Interleukin-15 Not determined. Not determined. Ph.D.-12 phage display library (X12) Bound phages were competitively eluted with recombinant goose IL-2.