101 CX7C fUSE5 phage display library 7 Erkki Ruoslahti Completely random Circular 102 CX10C phage display library 10 6.2e8 Xiaomin Fan Completely random Circular 103 pAFF/MBP vector-based phage display library 8 3.0e9 1.0e12 A. Gonzalez-Techeraa Completely random Circular This library, with the general sequence ASGSACX8CGP6, expresses cyclic 8 mer random peptides flanked by two cysteines and fused to the phage coat protein pIII. 104 pComb3 X15 phage display library 15 1e10 H. Pannekoek, Amsterdam Medical Centre, Amsterdam, The Netherlands Completely random Linear 105 pComb8 CX15C phage display library 15 1e10 H. Pannekoek, Amsterdam Medical Centre, Amsterdam, The Netherlands Completely random Circular 106 X13 phage libray 13 1e8 1e12 Dyax Corp Completely random Linear This library displays 13 amino acids of randomized sequence equimolar at each position for all amino acids except cysteine. 107 CX7C T7 phage display library 7 5e8 1e9 Hai-yan Hong Completely random NNK Circular The phage library displayed CX7C (C, cysteine; X, any amino acid residue) random peptides, which was built in the T7 415-1b vector (Novagen) between EcoRI and HindIII sites. X residues are encoded by NNK. 108 X6 phage display library 6 2.0e8 4.5e10 M Trepel Completely random NNK Linear 109 CX7C phage display library 7 M Trepel Completely random NNB Circular 110 Tat-based pCANTAB-5E library library 5 Haruhiko Kamada Semi-random Linear The library has a framework as GX5QX3PX2, randomizing ten amino acids within the Tat transduction domain. 111 CX7C T7 phage display library 7 1.0e8 1e9 Novagen Completely random NNK Circular This library is based on T7 415-1b phage vector. 112 X9 phage display library 9 1.0e10 Carla Lo Passo Completely random Linear 113 X12 phage display library 12 1.0e10 Carla Lo Passo Completely random Linear 114 X7-LXXLL-X7 M13 phage display library 16 Niharika B. Mettu Semi-random Linear 115 SS-X16-S M13 phage display library 16 D. J. Kenan (Duke University) Completely random Linear 116 X8 and CX8C phage display library pool 8 Anthony N. Hodder Completely random The pool consists two eight-residue libraries displaying peptides on M13 gene VIII coat protein. One is a linear library with all 8 residues randomized. Another is a cyclic library with all 8 residues randomized in the context of a pair of fixed cysteine residues. In the cyclic library, the spacing of the cysteine residues varied from three to six residues. The randomization of residues for both libraries was achieved using Kunkel mutagenesis, employing degenerate oligonucleotides in which each randomized residue was encoded by an NNS codon. Equal aliquots of each linear and cyclic sublibrary were combined, giving rise to the 8-residue pool for panning. 117 X14 and CX14C phage display library pool 14 Anthony N. Hodder Completely random The pool consists two 14-residue libraries displaying peptides on M13 gene VIII coat protein. One is a linear library with all 14 residues randomized. Another is a cyclic library with all 14 residues randomized in the context of a pair of fixed cysteine residues. In the cyclic library, the spacing of the cysteine residues varied from three to six residues. The randomization of residues for both libraries was achieved using Kunkel mutagenesis, employing degenerate oligonucleotides in which each randomized residue was encoded by an NNS codon. Equal aliquots of each linear and cyclic sublibrary were combined, giving rise to the 14-residue pool for panning. 118 Display PHAGE system library (CX6C) 6 3e7 1e12 Display System Biotech (Vista, California) Completely random Circular 119 fUSE55-based X8 phage display library 8 1.1e8 George Georgiou Completely random NNK Linear The rondom sequence was inserted in gIII. 120 TN phage display library pool 9-12 Dyax Corp Semi-random Linear TN-pooled library is composed of TN-9-IV (3.2e9 independent transformants), TN10-X(2e9 independent transformants) and TN-12-I (1.4e9 independent transformants). 121 CX9C M13 phage display library 9 7.0e8 Kerry S. Kelleher Completely random Circular The CX9C library was constructed using the M13 filamentous bacteriophage peptide display cloning system from New England Biolabs. Expressing the peptide as a fusion with the M13 minor coat protein gene III presents as many as five copies of the peptide clustered on the surface of the bacteriophage. 122 CX9W1-9C M13 phage display library 9 Kerry S. Kelleher Semi-random Circular The CX9W1-9C library was designed to contain at least one tryptophan residue in every phage displayed peptide. This library was constructed using the M13 filamentous bacteriophage peptide display cloning system from New England Biolabs. Expressing the peptide as a fusion with the M13 minor coat protein gene III presents as many as five copies of the peptide clustered on the surface of the bacteriophage. 123 LX10 phage display library (CX10C) 10 Mark Paetzel Completely random Circular Random 10-mer peptide sequence constrained by two conserved cysteines was displayed on the pVIII of the f88 phage. 124 X6 T7 phage display library 6 1.5e8 Morley D. Hollenberg Completely random NNK Linear The sequence of synthetic degenerate oligonucleotides inserted in the coding region of T7 phage capsid protein (employing T7 Select 1-1 vector arms, T7 Select system, Novagen), encoding a random hexamer followed by (His)6 tag. 125 fUSE5-based X15 phage display library 15 Dorothee Herlyn Completely random Linear