101 FHLPYNHNWFAL(19) 1 Phage display (subtractive panning) 3 PMID:15618169 Anti-type 8 PS monoclonal antibody NAD Type 8 capsular polysaccharide Not determined. Not determined. Ph.D.-12 phage display library (X12) Between panning rounds 1 and 2, a subtractive panning was performed on a blank, blocked polystyrene plate to remove nonspecific plastic binders. Inhibition studies showed that FHLPYNHNWFAL is a mimetic of type 8 PS. 102 HPTLLRI(5) SLPTLTL(1) PVQMPWL(1) HAIYPRHH(1) HWGMWSY(1) ITNDFKQ(1) 6 Phage display (common panning) 4 PMID:15162530 Anti-NE2 monclonal antibody 8C11 Capsid protein Not determined. Not determined. Ph.D.-7 phage display library (X7) 103 SILPYPY(3) ALWGPTS(1) LGTLPSA(1) SPKMPIL(1) FSYLPSH(1) STLFMNF(1) SPPNVR(1) SAHVLST(1) 8 Phage display (common panning) 4 PMID:15162530 Anti-NE2 monclonal antibody 8H3 Capsid protein Not determined. Not determined. Ph.D.-7 phage display library (X7) 104 MHGPTKNQMWHT(7)[0.687/0.814] 1 Phage display (common panning) 3/4 PMID:15207087 Anti-hantaan virus monoclonal antibody F3 Hantaan virus Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Phage clones F3-8A with the sequence of MHGPTKNQMWHT and F3-8B harboring the sequence of MHGPTKNQMWHT were tested by ELISA for their binding specificity to anti-hantaan virus monoclonal antibody F3. The absorbance was determined as A450/A630 nm. Firstly the mAb F3 (100 mg/L) was incubated with phages. When the titer of mAb diluted 400 times, the absorbances were measured and shown. 105 MHRHAHPMYQPP(3)[0.814] MHRHYHPWLTPS(2)[NT] FHRTPWLQFSLP(2)[0.887] WPKHFHIPQWWT(1)[1.134] 4 Phage display (common panning) 3/4 PMID:15207087 Anti-hantaan virus monoclonal antibody B11 Hantaan virus Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Selected phage clones were tested by ELISA for their binding specificity to anti-hantaan virus monoclonal antibody B11. The absorbance was determined as A450/A630 nm. Firstly the mAb B11 (100 mg/L) was incubated with phages. When the titer of mAb diluted 400 times, the absorbances were measured and shown. NT denotes not tested. 106 CLPWSDGPC(5) CLPWGTGPC(1) 2 Phage display (common panning) 3 PMID:15564686 Anti-gibberellin monoclonal antibody 8E9 Gibberellin 3-beta-dioxygenase 1 Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 107 CTPEQQFTC(8) CTKSPPLQC(4) CTPSALASC(3) CSWPNTSNC(2) CHATHTNYC(1) CTSLLRGQC(1) CPPMPNTTC(1) 7 Phage display (common panning) 3 PMID:17037189 Anti-SARS-CoV spike glycoprotein monoclonal antibody 2C5 Spike glycoprotein Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The results demonstrated that TPEQQFT is a mimic epitope peptide containing neutralizing MAb 2C5. 108 ASYPPRL ISYHDLR SLSPGLP STSFTPH MADRIGT FAGVPSW LNNQFWY TPGQYWA YTRPLPT FSAPVRY 10 Phage display (competitive panning) 7 PMID:16867257 Anti-IFN-alpha-2b polyclonal antibody Interferon alpha-2 Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted for IFN- Twenty-three positive clones were obtained after seven rounds of selection. Ten clones were randomly picked from the positive clones and were sequenced. The corresponding amino acid sequences suggested 3 groups homologous to the 3 domains of IFN-α2b, defined by residues 24–41, 43–49, and 148–158 of IFN-α2b. 109 NMFGSLTSHVTA(4) QVNQWSPLVNIR(3) QLYNNRSLFPAW(2) NGNHRTLSAHAH(2) LGNQGLSLTLRL(1) VNPHHMQLPKLH(1) DENLSLRPLFPK(1) QAENKIDVHLPI(1) N-x(3,5)-L 8 Phage display (common panning) 3 PMID:16186382 Glucokinase regulatory protein Glucokinase Not determined. Not determined. Ph.D.-12 phage display library (X12) 110 CSERSMNFC(18) CYGLPHKFC(12) CPSGAARAC(9) CLQHKSMPC(3) CVKSMVTHC(3) CSQRSMNFC(2) CQPLRHHQC(2) CLPHKSMPC(1) CPSGTARAC(1) CKQRPAWLC(1) CIPMNAPWC(1) CSLPFARNC(1) CGPARLSFC(1) CMGLPLRFC(1) [RK]-S-M, L-[PQ]-H-K, P-S-G-[AT]-A-R-A 14 Phage display (common panning) 3 PMID:15506167 Human airway epithelial cell 1HAEo-cells Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 111 KGSHEALHRHIR[0.556, 0.953] KPHSTHLPAHQP[0.255, 0.795] HTIHSKQSHKNS[1.920, 1.206] HSKLNNRHHALL[0.803, 1.112] TPHSKPHFHKLP[0.193, 0.876] HSRHNHYLHPWA[0.477, 0.974] HSKHTNHPPVGS[0.260, 0.849] MPKHIHRPHNIN[1.184, 1.007] WTVLPHEGGLSS[0.473, 1.381] LPSRHAAHPHVR[0.351, 0.906] KSLSRHDHIHHH[0.577, 0.775] 11 Phage display (subtractive panning) 3 PMID:15041534 Anti-hTERT polyclonal antibody Telomerase reverse transcriptase Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA The binding of anti-hTERT polyclonal antibody with selected phages was quantified by both direct ELISA and sandwich ELISA methods. The absorbance was measured at 490 nm. Data shown were reproduced from the graph. In each round, a subtractive panning was performed on normal mouse IgG. After 3 rounds of screening, 13 of 24 randomly selected phage clones were identified as positive clones that could specifically bind to anti-hTERT anibody but not to normal mouse IgG. 112 WCRGGWC(4)[++] CLCAEGWC(4)[++] CHPGTGWC(3)[++] WCKSGWC(2)[++] WCVKGWC(2)[NT] SYCREGWC(1)[+] CGCRDGWC(1)[+] CLCHGGWC(1)[+] CRDGWCYS(1)[NS] CVAILKDC(1)[-] 10 Phage display (common panning) 3 PMID:14764731 Anti-CD47 monoclonal antibody C5D5 Leukocyte surface antigen CD47 Not determined. Not determined. CL6 phage display library (CX6C) ELISA ELISA results quantifying binding of such phage to mAb C5D5 are also shown with OD values ++ (>1.0), + (<1.0), - (background), NT (not tested) and NS (not significant). 113 CGWRNSFGQSLC(32)[++] CERVIGTGWVRC(6)[++] CHRVPGHGWVRC(5)[++] CVPVCREGWCGC(3)[NT] CYKSMDGWVVPC(3)[++] CVENVDGWTVPC(2)[++] CSWQHQDGWVWC(1)[++] CRVPETGWVKC(1)[NT] CRLMLNGWVVPC(1)[NT] CCRDGWCHHDWC(1)[++] CCREGWCGDGLC(1)[NT] CGWRNALQVVC(1)[NT] CGWRNLEGGSVC(1)[NT] CGWRDDSGQSMC(1)[NT] CRRVIGRVGCGC(1)[++] 15 Phage display (common panning) 3 PMID:14764731 Anti-CD47 monoclonal antibody C5D5 Leukocyte surface antigen CD47 Not determined. Not determined. CL10 phage display library (CX10C) ELISA ELISA results quantifying binding of such phage to mAb C5D5 are also shown with OD values ++ (>1.0), + (<1.0), - (background), and NT (not tested). Studies indicated that peptide CERVIGTGWVRC specifically blocked CD47 and SIRPα binding in a dose-dependent fashion. Protein binding assays using SIRPα domain-specific recombinant proteins demonstrated that this peptide directly bound to the distal-most Ig loop of SIRPα, the same loop where CD47 binds. 114 WLHLHSRPLPSTPHD(17)[220] AGDRPLPPLPYNPKS(8)[ND] LALARPLPVPPWRQI(2)[ND] TGPRPLPLPPLRSMS(2)[180] SFRPLPPLPQFLPMY(2)[185] STLMKISNRPLPAAS(2)[>10,000] RPGDPLRTPIAGDT(2)[>10,000] HSHFHPRPLPPLPVR(1)[417] FVGDPLPYIPHMHWF(1)[>10,000] R-P-L-P-x(2)-P 9 Phage display (common panning) 4 PMID:7929055 Src SH3 domain of Proto-oncogene tyrosine-protein kinase Src Not determined. Not determined. Not determined. X15 phage display library Binding assay Individual peptides were immobilized via their amino termini to polystyrene plates. A constant input of 32P-labeled GST/PKA/Src SH3 (typically 1-3 nM), with varying amounts of unlabeled GST/PKA/Src SH3 (0-10 μM), was incubated with the immobilized peptide. Scatchard analysis of binding data was performed using the EBDA and modified LIGAND programs (Biosoft). Kd (nM) values were determined by Scatchard analysis of equilibrium binding data. ND, not determined. In fact, GST/PKA/SrcSH3 fusion protein is the target. 115 RSLPPIPG(10) 1 Phage display (common panning) 8 PMID:7988556 Src SH3 domain of Proto-oncogene tyrosine-protein kinase Src Not determined. Not determined. Not determined. GX6G phage display library A point mutation in a flanking codon (GGG-AGG) had occurred, converting glycine to arginine. 116 SLARPL(1) TSMRPL(1) KSERPL(1) TLGRPL(1) SIARPL(1) APRIPL(1) LHRRAL(1) YDHRSL(1) LRQRPL(1) PASRPL(1) ARDRPL(1) PRSRPL(1) FVSRPL(1) NKGRSL(1) NRLRPL(1) LANREL(1) PTRRPL(1) x(3)-R-P-L 17 Phage display (common panning) 3 PMID:7988556 Src SH3 domain of Proto-oncogene tyrosine-protein kinase Src Not determined. Not determined. Not determined. X6PPIP phage display library 117 RLLKPL(2) IQHRLL(1) QSRRSL(1) AKRAPL(1) TGGRPL(1) HHIRPL(1) SYPRPL(1) HSTRAL(1) x(3)-R-P-L 8 Phage display (common panning) 3 PMID:7988556 Fyn SH3 domain of tyrosine-protein kinase Fyn Not determined. Not determined. Not determined. X6PPIP phage display library 118 PTHRIL(1) RNFLIL(1) RVSYNL(1) PVLHSL(1) RYLRPL(1) RSSRPL(1) VNKRPL(1) RSNRPL(1) RAKRPL(1) SHRHPL(1) R-x(2)-R-P-L 10 Phage display (common panning) 3 PMID:7988556 Lyn SH3 domain of tyrosine-protein kinase Lyn Not determined. Not determined. Not determined. X6PPIP phage display library 119 ASTRPL(2) RLFRPL(1) RPQRPL(1) RFKRPL(1) RAKRPL(1) RPYRPL(1) RIPRPL(1) RSLRPL(1) R-x(2)-R-P-L 8 Phage display (common panning) 3 PMID:7988556 PI3K SH3 domain of PI3-kinase subunit p85-alpha Not determined. Not determined. Not determined. X6PPIP phage display library 120 PPPYPP(5) APPYPP(2) PPAYPP(2) APSYPP(2) APHYPP(1) PPPYHP(1) PPSYPP(1) APNYPP(1) APSYSP(1) PPHYPP(1) P(3)-Y-P(2) 10 Phage display (common panning) 3 PMID:7988556 Abl SH3 domain of tyrosine-protein kinase ABL1 Not determined. Not determined. Not determined. X6PPIP phage display library 121 PPLPPP(3) PPLPGS(2) PPLPRP(1) PPLPSP(1) PPVPPP(1) PPLPNG(1) P(2)-L-P-x-P 6 Phage display (common panning) 3 PMID:7988556 Src SH3 domain of Proto-oncogene tyrosine-protein kinase Src Not determined. Not determined. Not determined. RSLRPLX6 phage display library 122 PPLPFD(1) PPPPFP(1) PPLPVP(1) PPLPWT(1) PPLPPL(1) PPIPGR(1) PPIPPP(1) PPLPAP(1) PPIPLG(1) PPIPDS(1) P(2)-[IL]-P-x(2) 10 Phage display (common panning) 3 PMID:7988556 Fyn SH3 domain of tyrosine-protein kinase Fyn Not determined. Not determined. Not determined. RSLRPLX6 phage display library 123 PPLPLR(2) PPLPAP(1) PPLPSP(1) PPLPLP(1) PPLPFP(1) PPLPPP(1) PPLPLL(1) PPLPQL(1) PPLPSA(1) P(2)-L-P-x-P 9 Phage display (common panning) 3 PMID:7988556 Lyn SH3 domain of tyrosine-protein kinase Lyn Not determined. Not determined. Not determined. RSLRPLX6 phage display library 124 PPLPPP(5) MVSLVP(1) LPFGPP(1) PPTPV(1) P(2)-L-P(3) 4 Phage display (common panning) 3 PMID:7988556 PI3K SH3 domain of PI3-kinase subunit p85-alpha Not determined. Not determined. Not determined. RSLRPLX6 phage display library 125 PPIPVR(1) PPVPYA(1) PPIPIL(1) PPVPYP(1) PPVPRS(1) PPVPGA(1) PPIPRF(1) PDIPLL(1) P(2)-[IV]-P-x(2) 8 Phage display (common panning) 3 PMID:7988556 Abl SH3 domain of tyrosine-protein kinase ABL1 Not determined. Not determined. Not determined. PPPYPPX6 phage display library