1151 CSDTRRGDC(23)[0.80] CKLGPIRGC(5)[0.60] CLTSHNMMC(3)[0.25] CPWTKAYHC(3)[NT] CNSYSLSRC(2)[NT] CRSHTPRSC(1)[NT] 6 Phage display (common panning) 3 PMID:14631075 Beta-lactamase TEM Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) ELISA Absorbance at 405 nm was measured. Data shown were reproduced from the graph. NT denotes not tested. 1152 PLFYSV KIPRTLT PLRLSW PRAVST KGPRQIT PRPLSG WPLGLA FRPRSIT RLPVGLT RSPKSLT PVWLAA IHPSSLTA PASFTS GQPHYLT MKPASWT TPAYMLT PLYLT PGLIGT PRSISN RLPASYT NPPRYLT PKTQIS SVPRHFT LLPAWLT THPYTMT LRPAKST SGPSTST GSGLKA AMGLKS KVGLRT GRRLIHH HPRRSIT GRRLLSR ALRRLET FYKRVLT FRRICV VFFRRQTA GLARNITA FGSRYLTA QDRYLNT 40 Phage display (common panning) 3 PMID:11279151 Matrix metalloproteinase-9, MMP-9 Not determined. Not determined. Not determined. X6 phage display library 1153 QHFNNSVNLGFT(11) SPERHLHDLRPY(1) NKLYPDAYFNPG(1) GGNWTAATASWA(1) RICMMLVIRSNA(1) 5 Phage display (subtractive panning) 2 PMID:11121403 BAG family molecular chaperone regulator 1 Heat shock cognate 71 kDa protein 1HX1, Not determined. Ph.D.-12 phage display library (X12) The recombinant Hap46 was immobilized on Petri dishes, blocked, and incubated with phage library following the manufacturer's protocol. To eliminate enrichment of phage clones interacting with the plastic surface or the blocking agent, the following two strategies were employed: (i) eluted and amplified material from the first panning was either subjected to a preadsorption step on bovine serum albumin coated Petri dishes prior to subsequent pannings, or (ii) bovine serum albumin as blocking agent was alternated with casein in the form of dried milk powder. 1154 QHFNNSVNLGFT(20) SPERHLHDLRPY(3) AILIISLMLGFT(1) APTVKNCPSPCP(1) YTTTWRPPYSHN(1) YWWIIIRQYTTP(1) TLATVPSSSLFV(1) LVDHHQTYTTPP(1) 8 Phage display (subtractive panning) 3 PMID:11121403 BAG family molecular chaperone regulator 1 Heat shock cognate 71 kDa protein 1HX1, Not determined. Ph.D.-12 phage display library (X12) The recombinant Hap46 was immobilized on Petri dishes, blocked, and incubated with phage library following the manufacturer’s protocol. To eliminate enrichment of phage clones interacting with the plastic surface or the blocking agent, the following two strategies were employed: (i) eluted and amplified material from the first panning was either subjected to a preadsorption step on bovine serum albumin coated Petri dishes prior to subsequent pannings, or (ii) bovine serum albumin as blocking agent was alternated with casein in the form of dried milk powder. 1155 IGATAPI(1) SNAYYPH(1) SSLPSVH(1) STPTRFP(1) 4 Phage display (in vivo) 1 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1156 LSTPPLL(2) MHLGDAQ(1) SGPRTLP(1) STYPRHM(1) 4 Phage display (in vivo) 2 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1157 KPSGLTY(1) LSTPPLL(1) SQMPARL(1) YPANLYP(1) YPTSKQS(1) 5 Phage display (in vivo) 3 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1158 APVVRFI(1) KPSGLTY(1) KVLPFYD(1) LSTPPLL(1) QAISRNA(1) 5 Phage display (in vivo) 4 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1159 KVLPFYD(3) LPLTPLP(2) SEAGSRY(1) SQMPARL(1) YPANLYP(1) 5 Phage display (in vivo) 5 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1160 EPPHRHA(1) SAIAQPR(1) SHGPQGY(1) SSFNGLH(1) 4 Phage display (in vivo) 1 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1161 HAIYPRH(4) NPLPLTS(1) 2 Phage display (in vivo) 2 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1162 HAIYPRH(2) GPPSPRY(1) KLTLPNR(1) LSTPPLL(1) 4 Phage display (in vivo) 3 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1163 HAIYPRH(2) KQTLPSA(1) SLPLFTR(1) TVHPPFS(1) 4 Phage display (in vivo) 4 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1164 HAIYPRH(4) ASSDLHS(1) KQTLPSA(1) YNHQRPP(1) YTGPYQH(1) 5 Phage display (in vivo) 5 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1165 KLPPPTF(1) LRFTATI(1) LSQTSGP(1) TVMSQRH(1) 4 Phage display (in vivo) 1 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1166 GLQNSLP(1) LMPSPRN(1) SQMPARL(1) SRTPIIH(1) VPSAPML(1) 5 Phage display (in vivo) 2 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1167 ASYTQPA(1) LPLTPLP(1) SHTTVNP(1) SQMPARL(1) TRTAEGH(1) 5 Phage display (in vivo) 3 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1168 ATFTHYK(1) FFPQPWA(1) KPSGLTY(1) SALPNLP(1) 4 Phage display (in vivo) 4 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1169 KPSGLTY(4) ASYTQPA(2) KQTLPSA(1) SPSAHPS(1) VRRQPAH(1) 5 Phage display (in vivo) 5 PMID:12852441 Polarized human umbilical vein endothelial (HUVE) cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phage library, diluted in PBS, were injected into the umbilical vein through the catheter. 1170 ARARS[7659] ARASE[2158] ERVSP[99] ETKRS[4358] GTLRF[5020] GVFRS[3545] IMSRQ[2512] KLRTT[9646] KTRSN[1917] LRMPT[5158] LRSRA[16926] LTTSK[1705] MTRSN[1772] PGRAP[3282] PSARM[5389] RGRMA[5765] RGRSE[14605] RNDKL[1196] RRSID[14982] SGRLA[3042] TGSRD[1417] TLQRV[3436] TRAPM[3299] TRDSR[2548] TVRMP[2289] VLRSP[2756] VRPLE[1248] GTVRS[NT] HRMSS[NT] ISPRS[NT] LGRSL[NT] MRNRA[NT] QWRNS[NT] RAAMM[NT] RLERV[NT] RPQEL[NT] RSGSV[NT] RVGPY[NT] SDRTA[NT] SSPRV[NT] VESRA[NT] 41 Phage display (common panning) 5-6, 8 PMID:12047384 Kallikrein-2 Not determined. Not determined. Not determined. X5 phage display library Fluorescence The CFP fluorescent substrate assay has been utilized to determine the kinetics of peptide substrate selection from a phage display library. Specificity constants (kcat/Km, 1/M × 1/s) were determined and shown. NT denotes not tested. The scissile bonds identified by phage display substrate selection correspond to those of the natural biological substrates of hK2, which include protein C inhibitor, semenogelins, and fibronectin. Moreover, three new putative hK2 protein substrates, shown elsewhere to be involved in the biology of the cancer, have been identified thus reinforcing the importance of hK2 in prostate cancer development. 1171 CPWYFWPC(4)[11.14] CPWYLAPC(2)[14.00] CPWYFLWC(2)[8.56] CWANGWPC(1)[20.26] CHGPLVWC(1)[14.27] P-W-Y-[FL]-W-P 5 Phage display (competitive panning) 3 PMID:11745373 C-C chemokine receptor type 3 Eotaxin Not determined. Not determined. CX6C phage display library Binding assay Results are expressed as percentage (mean ± SD) of (125)I-eotaxin binding. Experiments were performed in duplicate and repeated three times. Data shown were reproduced from the data. In fact, CCR3-transfected murine pre-Bcells were the target. Bound phage were first specifically eluted by competition with excess of eotaxin. 1172 MTRSIA(7)[17.71] LTDYQM(1)[17.16] VTPRQR(1)[11.95] LSSRKI(1)[15.35] 4 Phage display (common panning) 3 PMID:11745373 Eotaxin C-C chemokine receptor type 3 Not determined. Not determined. X6 phage display library Binding assay Results are expressed as percentage (mean ± SD) of (125)I-eotaxin binding. Experiments were performed in duplicate and repeated three times. Data shown were reproduced from the data. 1173 TFAPRWTQSWLP GRLAQYLHHPPT ARMTQLPFAALW GRWMHQPLPQLL GRMTQTSTLLLA GRMMTSIVPLPQ ARMTQTAAFLYT GRLTQTLGYQAQ ARWTQGLPAWGH GRLQPPSILNLW GVGRSLLFRLR SNMHFWNRFFEL DRTPWPRWLLSY 13 Phage display (common panning) 4 PMID:11330351 Botulinum neurotoxin A light chain Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 1174 AARHPTMHPSSS RLKKELRLPLAP NKWPLAHSQKKR SLTARQPPRVSW QPTLMTGATMHG FHRHHHSQHLPR HLKHFHHHSPNY KNLHHHHPHRSS KTMHRHHMHWSP HFKHHKSLPHLG 10 Phage display (common panning) 4 PMID:11330351 Botulinum neurotoxin B light chain Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 1175 RLKKELRLPLAP KSPKHSSSALHF KSRFRALGASLS LPTQNATVWPQR WHRLRGRITKSG RTRFPYGLRPVR TKRRPRTSPLGL 7 Phage display (common panning) 4 PMID:11330351 Botulinum neurotoxin C1 light chain Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12)