1126 CDSAFVTVDWGRSMSLC(11) CVVLHVPHGAEAGLPLY(4) 2 Phage display (common panning) 4 PMID:15203927 Human submucosal-gland carcinoma, Calu-3 cells Not determined. Not determined. Not determined. CX15C phage display library Biopan the phage library on differentiated Calu-3 cell monolayers, the membrane associated phage were degraded by proteinase K digestion and the internalising phage were protected from intracellular degradation by the endosomolytic compound chloroquine, amplified from cell lysates and used in the next round. 1127 FEKYYYW(25)[3.762 ± 0.144] TDRPALS(8)[0.752 ± 0.054] VMQATHD(5)[0.612 ± 0.078] WHTNYEP(3)[0.603 ± 0.065] KVFNWPW(3)[1.891 ± 0.047] YNPLPGT(3)[0.716 ± 0.064] APLESNW(2)[0.672 ± 0.044] LSLPKLP(2)[2.003 ± 0.047] KLWVIPQ(2)[2.081 ± 0.027] LQPMWFA(1)[3.491 ± 0.135] LTYRISP(1)[1.828 ± 0.066] FQNNLQL(1)[1.335 ± 0.039] GSFYVFP(1)[1.185 ± 0.046] LVYPPMV(1)[1.423 ± 0.025] ITPYTVL(1)[1.301 ± 0.049] LPPLNYY(1)[0.801 ± 0.073] HYTSATL(1)[0.552 ± 0.022] WQPNTRP(1)[0.728 ± 0.031] IPRTYPL(1)[0.625 ± 0.010] SAPGLLH(1)[0.615 ± 0.038] DYHNHLT(1)[0.586 ± 0.051] SPTQSTL(1)[0.580 ± 0.041] AQAIMQY(1)[0.517 ± 0.051] HHVKFQN(1)[0.623 ± 0.064] SLCSVLC(1)[0.580 ± 0.012] HTTYFPM(1)[0.552 ± 0.024] HVLPPLH(1)[0.810 ± 0.056] LHAWQDL(1)[0.773 ± 0.045] TGNPPPN(1)[0.745 ± 0.070] LSNYTRP(1)[0.712 ± 0.038] HTPLATA(1)[0.718 ± 0.037] HMPNASF(1)[0.697 ± 0.043] YNPYTPL(1)[0.670 ± 0.046] STLLPES(1)[0.690 ± 0.061] NVNLLLP(1)[0.635 ± 0.022] QANPLMI(1)[0.636 ± 0.019] NTTTYPT(1)[0.525 ± 0.038] 37 Phage display (common panning) 4 PMID:12486713 Cell division protein ftsA Cell division protein ftsA Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA Absorbance at 450 nm was measured. Data shown were average ELISA values (OD450nm) ± standard deviation (n = 4). The isolated peptides defined a degenerate consensus sequence, which in turn displayed a striking similarity with residues 126-133 of FtsA itself. This result suggested that residues 126-133 were involved in homodimerization of FtsA. 1128 VHALES DRVTLG LGGLSA CGLSLK GLSARH SKHALE VFALEG EVISGL LISGVL ELLSGL GRLSGSL AMSAGV G(2)-L-S-G-L, HALE, ISGL, MSAG, R-V-T-x-G 12 Phage display (subtractive panning) 3 PMID:12629410 Human urothelial cells Not determined. Not determined. Not determined. X6 phage display library Each library was incubated with MOLT-4 leukemia cells for 30 minutes on ice to deplete background and common cell surface binding phage (pre-clearing). 1129 SAHGTSTGVRGP(4) SVHMINTSQVHL(1) SDTTMGQVHRHP(1) KQASNLIVMHYP(1) YSLPQRTHLHLP(1) HLPTSSLFDTTH(1) HQNLNLGSSWST(1) 7 Phage display (common panning) 4 PMID:11478946 Allergen-specific IgE-mimicking monoclonal antibody 2A1 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 1130 VDLPEHGK(9) VGLPEHTQ(5) VGLPEHSA(4) VDLPTHSS(7) VDLPEHRQ(1) VDLPTHQS(1) VDLPTHNQ(1) VDLPQHGQ(1) DTTKNGSG(1) V-[DG]-L-P-[ET]-H 9 Phage display (subtractive panning) 4 PMID:14617786 RG2 rat glioma cells Not determined. Not determined. Not determined. f8-8mer phage display library (X8) RG2 cells were grown to a sub-confluent monolayer. First, an aliquot of the primary library in washing/blocking buffer was added to an empty flask (depletion flask) to remove plastic-binding phage clones. Buffer containing phage that did not bind to plastic was transferred to the flask with RG2 glioma cells for incubation. After that, phage not associated with tumor cells were washed away. Cell-surface bound phage were recovered with low pH elution buffer. 1131 ELRGDSLP(10) EVRGDSLP(2) VDLPSHPE(1) VNLPEHPE(2) VDLPRSDT(1) HTTKEQMA(1) V-[DG]-L-P-[ET]-H 6 Phage display (subtractive panning) 4 PMID:14617786 RG2 rat glioma cells Not determined. Not determined. Not determined. f8-8mer phage display library (X8) RG2 cells were grown to a sub-confluent monolayer. First, an aliquot of the primary library in washing/blocking buffer was added to an empty flask (depletion flask) to remove plastic-binding phage clones. Buffer containing phage that did not bind to plastic was transferred to the flask with RG2 glioma cells for incubation. After that, phage not associated with tumor cells were washed away. Cell-surface bound phage were recovered with lysis buffer. 1132 VDLPQHGG(4) VDLPTHTS(1) VNLPEHAQ(2) VGLPEHQP(1) DTTKTSAG(1) DSTKIGTS(1) DSTKASDA(1) DTTQSMHT(1) DSTKSTNS(1) DSTKAVAL(1) DSTKSGNM(1) DTTKGPGT(1) DGTKMAGG(1) V-[DG]-L-P-[ET]-H 13 Phage display (subtractive panning) 4 PMID:14617786 RG2 rat glioma cells Not determined. Not determined. Not determined. f8-8mer phage display library (X8) RG2 cells were grown to a sub-confluent monolayer. First, an aliquot of the primary library in washing/blocking buffer was added to an empty flask (depletion flask) to remove plastic-binding phage clones. Before incubation with RG2 cells, preselection steps with fibroblasts, myoblasts, and hepatocytes were included in the protocol to remove phage binding to these normal cells. Phage associated with RG2 cells were recovered from a single flask with elution buffer. 1133 DTTKGGNP(1) DDTKHSLP(1) DTTRTHMP(1) DSTRGSPA(1) DSTRTTSA(1) DTTRLSDQ(1) DNTRVAAP(1) DDTRYSSA(1) DETLYGIS(1) DYDMTKNT(1) D-[TS]-T 10 Phage display (subtractive panning) 4 PMID:14617786 RG2 rat glioma cells Not determined. Not determined. Not determined. f8-8mer phage display library (X8) RG2 cells were grown to a sub-confluent monolayer. First, an aliquot of the primary library in washing/blocking buffer was added to an empty flask (depletion flask) to remove plastic-binding phage clones. Before incubation with RG2 cells, preselection steps with fibroblasts, myoblasts, and hepatocytes were included in the protocol to remove phage binding to these normal cells. Phage associated with RG2 cells were recovered from a single flask in two sequential steps, first with elution buffer and then with lysis buffer. 1134 DLTKSTAP(9) DTTKSTTT(1) EPVQPHST(1) D-[TL]-T-K 3 Phage display (subtractive panning) 6 PMID:14617786 RG2 rat glioma cells Not determined. Not determined. Not determined. f8-8mer phage display library (X8) RG2 cells were grown to a sub-confluent monolayer. First, an aliquot of the primary library in washing/blocking buffer was added to an empty flask (depletion flask) to remove plastic-binding phage clones. Before incubation with RG2 cells, preselection steps with fibroblasts, myoblasts, and hepatocytes were included in the protocol to remove phage binding to these normal cells. Phage associated with RG2 cells were recovered from a single flask with elution buffer. 1135 ESRGDSYA(2) DLTKSSAP(1) DTTKLTND(1) DNAIYTYQ(2) D-[TL]-T-K 4 Phage display (subtractive panning) 6 PMID:14617786 RG2 rat glioma cells Not determined. Not determined. Not determined. f8-8mer phage display library (X8) RG2 cells were grown to a sub-confluent monolayer. First, an aliquot of the primary library in washing/blocking buffer was added to an empty flask (depletion flask) to remove plastic-binding phage clones. Before incubation with RG2 cells, preselection steps with fibroblasts, myoblasts, and hepatocytes were included in the protocol to remove phage binding to these normal cells. Phage associated with RG2 cells were recovered from a single flask in two sequential steps, first with elution buffer and then with lysis buffer. 1136 QQGRGDIDSR(3) AQCRSDSPSC(3) RQGRGDQRST(3) TQRGDTIDSK(1) VQRGDAPNSS(1) AQRGDSYTSE(1) VQRGDEVLSK(1) AQRGDQLDSY(1) RQRGDGPYSQ(1) RGD 9 Phage display (common panning) 3 PMID:12646694 Integrin alpha-V-beta-3, integrin αvβ3 Not determined. Not determined. Not determined. Tendamistat loop I phage display library (xQxxxxxxSx) 1137 RARGDNPSNL(15) 1 Phage display (common panning) 3 PMID:12646694 Integrin alpha-IIb-beta-3, integrin αIIbβ3 Not determined. Not determined. Not determined. RGDX phage display library (xxRGDxxxxx) 1138 GARGDGNPWE(1) ASRGDRPQDM(1) TSRGDHPRTQ(1) GSRGDSLIMH(1) ASRGDNPLLW(1) TSRGDNPQTM(1) VGRGDSPRNA(1) VTRGDNYYPM(1) ASRGDTPRSY(1) ASRGDTYTTW(1) ATRGDDPRPR(1) VSRGDMPPMW(1) ATRGDEYQPR(1) VSRGDSYIGE(1) VTRGDTYWRK(1) 15 Phage display (common panning) 3 PMID:12646694 Integrin alpha-V-beta-3, integrin αvβ3 Not determined. Not determined. Not determined. RGDX phage display library (xxRGDxxxxx) 1139 ARRGDTFSLL(1) VSRGDTYVES(1) VTRGDTFTQS(1) VARGDTYVAS(1) ATRGDYYVAS(1) RSRGDTPARF(1) VRRGDVPFSS(1) ATRGDTILYL(1) VTRGDTFTIS(1) ATRGDTIEYE(1) AQRGDTVEYF(1) FARGDSLPEY(1) VTRGDSISYL(1) GSRGDTFRLV(1) VSRGDTFSLI(1) 15 Phage display (common panning) 3 PMID:12646694 Integrin alpha-V-beta-5, integrin αvβ5 Not determined. Not determined. Not determined. RGDX phage display library (xxRGDxxxxx) 1140 RKSRGDMGIY(2) HRTRGDMGSY(2) RYTRGDLGLT(1) RHSRGDMGSS(1) HRSRGDMGYW(1) RKSRGDAGLM(1) RTGRGDMGAN(1) RRTRGDLGLV(1) RSSRGDLGLM(1) RMSRGDLGLY(1) RKSRGDYGFV(1) RRSRGDTGIV(1) RTSRGDLGTF(1) 13 Phage display (common panning) 3 PMID:12646694 Integrin alpha-IIb-beta-3, integrin αIIbβ3 Not determined. Not determined. Not determined. XRGD phage display library (xxxRGDxxxx) 1141 HHARGDKDHM(1) ALGRGDSDLI(1) GSGRGDADLH(1) HPARGDSDFE(1) RAARGDSDHR(1) RHSRGDSDHL(1) RPSRGDTDHY(1) RHARGDFDHA(1) RVPRGDSDLT(1) RPGRGDSDQY(1) RQGRGDSRTS(1) RHARGDNDTR(1) RPSRGDMDHM(1) QPARGDMDLR(1) RSARGDSDHR(1) 15 Phage display (common panning) 3 PMID:12646694 Integrin alpha-V-beta-3, integrin αvβ3 Not determined. Not determined. Not determined. XRGD phage display library (xxxRGDxxxx) 1142 HLARGDDLTY(15) 1 Phage display (common panning) 3 PMID:12646694 Integrin alpha-V-beta-5, integrin αvβ5 Not determined. Not determined. Not determined. XRGD phage display library (xxxRGDxxxx) 1143 STHEETP(6) STHEETS(5) AYHTFTP(2) STHHSTP(2) SPHEETP(1) ATHEETP(1) SPHESYP(1) SPHESYL(1) SGRMMRL(1) WEFTFPP(1) STHEEHP(5) STPHSTP(3) STAEEHP(2) SPPEETP(3) ATAHSHP(1) STHHSHP(1) TQPHHTP(1) SMYQPPM(1) TPPHSTP(1) SPPYLTN(1) S-T-x(4)-P 21 Phage display (competitive panning) 3 PMID:12388644 Peripheral-type benzodiazepine receptor Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) In fact, isolated mitochondria from the MA-10 mouse Leydig cell tumor line, which express high amounts of PBR, was the target. In biopanning experiments, PBR-interacting phage peptides were eluted using either PK 11195 or Ro5-4864. 1144 SLWPPVS(44) MSPILSP(26) ITQPSLR(8) FKSLPMP(8) GRPLPPS(5) QSTQSPL(5) AHNSAAD(5) AEGPPNE(4) FGSHASE(3) SAALPAR(3) NWLPTPP(2) LVSTPLP(2) KVPWISW(2) SARLPAR(2) VAKIWVD(2) TSTTHTP(2) GLSPATS(2) MSKPTPL(1) IQPFTLQ(1) SAGLPAR(1) IVYRTSS(1) TYQRALY(1) NRTMAPW(1) RSMQMPP(1) TMSLPHH(1) 25 Phage display (common panning) 3 PMID:11994302 Chaperone protein hscA Iron-sulfur cluster assembly enzyme ISCU, mitochondrial Not determined. Not determined. Ph.D.-7 phage display library (X7) Screening of a heptameric peptide phage display library revealed that Hsc66 prefers peptides with a centrally located Pro-Pro motif. 1145 GNNSVSKEKPPSLNWP(1) TELKLAPPVLNAPPL(2) KAHPPLLLNSPRDVPL(1) YPKESPPRLNAPWYQ(1) ESKLTPPPLNPIRVV(2) KPRDTLPPPLNRPPP(3) VPVDAPHAGTKPHSA(2) K-x(2)-P(2)-x-L-N-x-P 7 Phage display (common panning) 3 PMID:11139210 Serum antibodies with Lyme disease Not determined. Not determined. Not determined. f88-15mer phage display library (X15) First-round affinity selections were carried out in four polystyrene 24-well dishes. Wells were coated with streptavidin. The second round was carried out in four 96-well ELISA dishes. A key difference was that neutravidin was substituted for streptavidin to capture Bio-IgG molecules. The third round of affinity selection was like the second round, but streptavidin was substituted for neutravidin. 1146 PKSSCTQNPILCAILS(1) 1 Phage display (common panning) 3 PMID:11139210 Serum antibodies with Lyme disease Not determined. Not determined. Not determined. f88-Cys6 phage display library (X4CX6CX4) 1147 SCPEGSKLCI(1) TCPEGAKLCD(1) SCAEGAKYCL(1) ACVAGDATCK(1) 4 Phage display (common panning) 3 PMID:11139210 Serum antibodies with Lyme disease Not determined. Not determined. Not determined. f88-LX6 phage display library (XCX6CX) 1148 EKRFACKPLCNTPA(2) 1 Phage display (common panning) 3 PMID:11139210 Serum antibodies with Lyme disease Not determined. Not determined. Not determined. f88-Cys3 phage display library (X5CX3CX4) 1149 QKDFACKHCKLPSP(2) RPDRLCPCVDPRE(1) K-x-F-A-C-K 2 Phage display (common panning) 3 PMID:11139210 Serum antibodies with Lyme disease Not determined. Not determined. Not determined. f88-Cys2 phage display library (X5CX2CX5) 1150 QRSECSTSKCFVRK(2) YREACTNGKCFVLK(2) R-x(2)-C-x(3)-K-C-F-V-x-K 2 Phage display (common panning) 3 PMID:11139210 Serum antibodies with Lyme disease Not determined. Not determined. Not determined. f88-Cys4 phage display library (X4CX4CX5)