1101 QLTMRLQ YGRETST SAESRHY M-x(2)-P 3 Phage display (competitive panning) 1 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1102 RPLPYPD LASPNGH GAASTRY M-x(2)-P 3 Phage display (competitive panning) 2 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1103 MANPGRA MQPSLVH MTTYPLS MTTTMQL MAVDPLR M-x(2)-P 5 Phage display (competitive panning) 3 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1104 MQSPPFH MTPPASI MTYPREF SARPVPL SPRPYST M-x(2)-P 5 Phage display (competitive panning) 4 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1105 MQLPPDT MQTPLAP MSLPLLP MTAPPAR MGNPINH MQISGHP LTRPVSP LAAPPKA DQSRMPT LAKVPPQ M-x(2)-P 10 Phage display (competitive panning) 5 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1106 QNLRGDS EAPAQLP AGPTWAH M-x(2)-P 3 Phage display (competitive panning) 1 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1107 MATPRNS MAARAPM AAAHALF M-x(2)-P 3 Phage display (competitive panning) 2 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1108 MQLPKSL MGKPLHS MSTPSKV LAQFPSP APFTHLR M-x(2)-P 5 Phage display (competitive panning) 3 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1109 MTLPMLQ MSQPPLA MWPTITP TALSRPP SATNQAP M-x(2)-P 5 Phage display (competitive panning) 4 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1110 MQLPVYP MQMPAVN MQPPGSI MATPGLG MAVPQPI MSARFPP LTRPPWG FTKTTSP GPTRILK VRWEMNL M-x(2)-P 10 Phage display (competitive panning) 5 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1111 TRLPHNP RYLVESP WPIRPLS M-x(2)-P 3 Phage display (competitive panning) 1 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1112 MMLHPYA LPSKLLD LVTAPRL M-x(2)-P 3 Phage display (competitive panning) 2 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1113 MAAAPFS MATASQL AAMPIIP LTTVPPY FAKDYSR M-x(2)-P 5 Phage display (competitive panning) 3 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1114 DNPRGFS FHPLNLS NNMAPHL VNRLSEN M-x(2)-P 4 Phage display (competitive panning) 4 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1115 MQLPLAT MGLPLGK MGLPPSP MTLPAAS MTSPPTP MSLSPPQ MGTLPTT LTLPPSP LSPPPTH STHPLQT M-x(2)-P 10 Phage display (competitive panning) 5 PMID:12032665 Fibroblast growth factor receptor 2, FGFR-2 Heparin-binding growth factor 2 (HBGF-2) 1EV2,1II4,1IIL, Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation. 1116 CCAVLNGAY(1) EGFGSGGAI(2) SQNSAEGAW(1) LTHCGSGAS(1) YCHTFHGAG(1) YHANSQGAV(1) EMLQGAGAM(1) PSMSLFGAF(1) EVLSHAGAH(1) GLRPGEGAY(1) IISCDEGVG(1) GLWWAEGVV(1) IVYVGDGVC(1) NLSVEGGVW(1) ARSLFGVD(1) GGFRIFGIV(1) GAGIGWGID(1) CVVGCGGGV(1) G-A-x 18 Phage display (common panning) 3 PMID:11983234 Alzheimer's disease specific monoclonal antibody MN423 Not determined. Not determined. Not determined. X9 λ phage display library 1117 NMMRFTSQPPNN(9)[0.40] NMMKYISPPIFL(9)[1.11] NMMNYIMDPRTH(3)[2.52] NMMRFTELSTPS(1)[0.99] 5 Phage display (common panning) 3 PMID:12682274 Anti-LOS polyclonal antibody Lipooligosaccharide, LOS Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA The binding reactivity was determined by an ELISA, in which each peptide was covalently coupled to NucleoLink strip as a coating Ag, followed by incubation with the target rabbit anti-LOS Ab. The binding reactivity of the synthetic peptides to the target Ab was evaluated by average OD405 values of each diplicate sample after incubation with a goat anti-rabbit IgG alkaline-phosphatase conjugate, and then addition of a substrate. 22 clones were subjected to DNA sequencing, and four consensus sequences were identified as NMMRFTSQPPNN, NMMNYIMDPRTH, NMMKYISPPIFL, and NMMRFTELSTPS. Three of the four synthetic peptides showed strong binding reactivity to the rabbit anti-LOS Ab and also a mouse bactericidal monoclonal anti-LOS Ab in vitro, and elicited specific serum anti-LOS Abs in rabbits (27- to 81-fold) after conjugation with keyhole limpet hemocyanin. 1118 SWTPDKWLRPSG(2)[3.14/3.03] NIAHPDRWDRLR(1)[3.14] ADNPIKWDRART(1)[3.26] LSNTANPWSWDR(1)[3.02] ASNWTDFRVTDR(1)[2.97] GMLPPQLFDRHH(1)[2.91] AEPFYSWDRASR(1)[3.07] P-x(2)-W-D-R 7 Phage display (subtractive panning) 4 PMID:12902494 Monoclonal antibody HC-10 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Reactivity of phage particles with mAb was measured by ELISA. Absorbance was read at 492 nm with a Multiscan microplate reader (Benchmark; Bio-Rad). At each round, phage particles binding to isotypic and allotypic determinants of mAb HC-10 were removed by a preadsorption step on mAb HC-10 isotype-matched mAb W6/32. 1119 HYQHNTHHPSRW(2)[3.9 ± 0.7] HFQAQMRHGHGH(1)[3.6 ± 0.5] HQSHHYGPRDHT(1)[3.4 ± 0.4] HIKHHPSSVPHA(1)[3.2 ± 0.3] GSPHHNHFKESH(1)[3.0 ± 0.1] HSPHIWSPHHGP(1)[3.0 ± 0.3] HLQIPKPHVHHT(1)[2.9 ± 0.5] HSVHHLPSPLSH(1)[2.8 ± 0.2] HPHQAHPSPKAH(1)[2.5 ± 0.3] HSPLGSHHHPKH(1)[2.5 ± 0.2] HNHAHLPLHPAP(1)[2.5 ± 0.6] HNHHPHSTRQAS(1)[2.4 ± 0.3] GTHVHHPHSTST(1)[2.4 ± 0.7] HMTELHHSGVHQ(1)[1.8 ± 0.4] 15 Phage display (common panning) 4 PMID:14644435 Zinc ion, Zn(2+) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Relative binding affinity of phage at 1 nM for metal ions was determined by ELISA. Absorbance was measured at 492 nm. The absorbance ratio of each clone to the primary phage library was estimated. All 14 peptide sequences contained four or five histidines, however, there was no obvious consensus in motif among the selected sequences. 1120 CWKLLGSEEEC(15) CPCFLLGCC(15) CWHKDLLGC(4) CWSMELLGC(1) CPPDLFWYC(4) CPEDLYFFC(3) CPEDFIFFC(1) L(2)-G 7 Phage display (common panning) 5 PMID:11381078 Integrin alpha-M-beta-2, integrin αMβ2 Not determined. Not determined. Not determined. CX7C and CX9C phage display library pool LLG sequences exposed on ICAM-1 and on von Willebrand factor at sites of vascular injury play a role in the binding of leukocytes, and CPCFLLGCC and peptidomimetics derived from it could provide a therapeutic approach to inflammatory reactions. 1121 ELRGDMAAL(3) SLRGDRAGW(2) ELRGDRAHW(1) YRDFRDIWA(1) E-L-R-G-D-[RM]-A-x-[WL] 4 Phage display (common panning) 3 PMID:11158220 Cortical collecting duct, CCD Not determined. Not determined. Not determined. LL9 phage display library (X9) 1122 ELRGDMAAL(5) ELRGDMAHW(2) LRGDAFSLA(1) E-L-R-G-D-[RM]-A-x-[WL] 3 Phage display (common panning) 4 PMID:11158220 Cortical collecting duct, CCD Not determined. Not determined. Not determined. LL9 phage display library (X9) 1123 ELRGDRAHW(6) EIRGDRAHW(1) EMRGDLAGF(1) E-L-R-G-D-[RM]-A-x-[WL] 3 Phage display (common panning) 3 PMID:11158220 Cortical collecting duct, CCD Not determined. Not determined. Not determined. LL9 phage display library (X9) 1124 KMGGTNHPE(4) MKTASVRHP(1) FTNHVSTRR(1) VLNHEMSIM(1) KMAGTNHPS(1) K-x(3)-T-N-H-P 5 Phage display (common panning) PMID:11158220 Proximal convoluted tubules, PCT Not determined. Not determined. Not determined. LL9 phage display library (X9) 1125 RRLGRQTYDNES HDEGRQIIQFEE LRNCEQDFFTLN AFAQAPTHQLSL ESNPVDGAHLSL PNEPDDLALMRIIRI 6 Phage display (common panning) 4 PMID:11874892 Anti-C.pneumoniae monoclonal antibody 8A6 Chlamydia pneumoniae Not determined. Not determined. f3-15mer phage display library (X15) The selected peptide mimotope sequences tended to be composed of charged residues surrounding a core of hydrophobic residues.