1076 LPALDPTKRWFFETK(21) AHWNSENTVVGLPSK(6) GMQMHQRHVYLSKRP(3) 3 Phage display (common panning) 3 PMID:11836009 Anti-estradiol monoclonal antibody E2-15 Estrogen Not determined. Not determined. X15 phage display library Phage particles was incubated with biotinylated mAb. The preincubated phage-mAb mixture was diluted with PBST and then transferred to the streptavidin-coated plate. More than 70% of the clones displayed the peptide sequence LPALDPTKRWFFETK. There was no consensus among the three peptide sequences. All three phage specifically bound mAb E2-15 in a dose-dependent manner, but failed to bind other tested anti-steroid monoclonal antibodies (anti-progesterone mAb and anti-testosterone mAb). 1077 KGTFDPLQEPRT(1)[1.59 ± 0.09] QDPLFPAMATWA(1)[NT] WDPLLPPFPPPN(1)[NT] SPMDRFDPLHLP(1)[NT] NNPYDALYNFPR(1)[NT] LSKHQFDALLPP(1)[NT] DSMDVFQPPPIW(1)[NT] SLFDVLWSPPPK(1)[NT] DPL, DAL, D-V-[LF] 8 Phage display (common panning) 3 PMID:11425742 Anti-BTx-A monoclonal antibody BT57-1 Botulinum neurotoxin type A, BoNT/A Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA The absorbance at 490 nm was read with a microplate reader. The synthetic peptide P4M (KGTFDPLQEPRT) corresponded to the phage-displayed peptide selected by BT57-1 and was able to bind the antibodies specifically. Data shown were reproduced from the graph. NT denotes not tested. 1078 MCIDCYLSTLGL(2) YIKPPVSCYDCQ(2) MCWDCTLHARSD(1) CYSMCLDCIMFG(1) C-x-D-C 4 Phage display (common panning) 3 PMID:11425742 Anti-BTx-A monoclonal antibody BT150-3 Botulinum neurotoxin type A, BoNT/A Not determined. Not determined. Ph.D.-12 phage display library (X12) 1079 CKIAK(1)[0.49] CQPMDQACDL(1)[0.46] CQPMEQACDL(1)[0.48] 3 Phage display (common panning) 4 PMID:12191519 Anti-glutamic acid decarboxylase monoclonal antibody GAD-6 Glutamate decarboxylase 2 Not determined. Not determined. C9C T7 phage display library ELISA Absorbance at 405 nm was measured with a microtitre plate reader (Molecular Devices). The mean OD of the antigencoated wells was corrected by subtracting the mean OD of the equivalent blank wells. Data shown were reproduced from the graph. 1080 ARRWDCDGHMCWAQI(3)[1.06 ± 0.04] 1 Phage display (common panning) 5 PMID:12191519 Anti-glutamic acid decarboxylase monoclonal antibody GAD-6 Glutamate decarboxylase 2 Not determined. Not determined. f88-Cys4 phage display library (X4CX4CX5) ELISA Absorbance at 405 nm was measured with a microtitre plate reader (Molecular Devices). The mean OD of the antigencoated wells was corrected by subtracting the mean OD of the equivalent blank wells. Data shown were reproduced from the graph. 1081 LCSTVHCCPGSST(1)[0.56] DPGRSNWSMSFD(1)[1.38] SPSFPLWSFSYL(1)[1.09] 3 Phage display (common panning) 3 PMID:12191519 Anti-glutamic acid decarboxylase monoclonal antibody N-mAb Glutamate decarboxylase 2 Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Absorbance at 405 nm was measured with a microtitre plate reader (Molecular Devices). The mean OD of the antigencoated wells was corrected by subtracting the mean OD of the equivalent blank wells. Data shown were reproduced from the graph. 1082 SFLQTEIDNMGR(1)[1.62] WDLTYELDRLWT(1)[1.06] GFLIWEVDTLSP(1)[1.72] 3 Phage display (common panning) 3 PMID:12191519 Anti-glutamic acid decarboxylase monoclonal antibody N-mAb Glutamate decarboxylase 2 Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Absorbance at 405 nm was measured with a microtitre plate reader (Molecular Devices). The mean OD of the antigencoated wells was corrected by subtracting the mean OD of the equivalent blank wells. Data shown were reproduced from the graph. 1083 AINTICSTPLCWNEA(1)[0.73] ATNRPCSTPMCMGSY(1)[1.24] ASHDSCSTPMCSTPR(1)[1.31] 3 Phage display (common panning) 6 PMID:12191519 Anti-glutamic acid decarboxylase monoclonal antibody C-mAb Glutamate decarboxylase 2 Not determined. Not determined. f88-Cys4 phage display library (X4CX4CX5) ELISA Absorbance at 405 nm was measured with a microtitre plate reader (Molecular Devices). The mean OD of the antigencoated wells was corrected by subtracting the mean OD of the equivalent blank wells. Data shown were reproduced from the graph. 1084 CLASRVSSFLGWGSNLC(5) CSASFFSWLFGWELAC(1) CESFWASRFSSLGGMAC(1) CSDMWLSRLLFTFLGWC(1) CVLVELASLVGLSLWEC(1) CLANGWGCFLGCWFHGC(1) CHVLAMVVGFLGVEWFC(1) FLGW, LASR 7 Phage display (common panning) 5 PMID:12183059 Vascular smooth muscle cells, VSMC Not determined. Not determined. Not determined. CX15C phage display library Phages were added to a monolayer of dedifferentiated, proliferating VSMC. 1085 CNIWGVVLSWIGVFPEC(3) CESLWGGLMWTIGLSDC(3) CSFLTRLGCEIGRFLRC(1) CDCTVRIGRVCMAASC(1) CNGVIFSWGRGWELAC(1) IGR 5 Phage display (in vivo) 6 PMID:12183059 Vascular smooth muscle cells, VSMC Not determined. Not determined. Not determined. CX15C phage display library Before in vivo selections, phages were selected by three rounds of biopanning on cultured VSMC. In this case, non-internalized phages were not removed, to ensure the largest possible variation within this pre-selection. After three rounds of additional in vivo biopanning, 84 clones were picked and grown individually in 96-well plates and tested for binding to VSMC in a phage binding assay. 1086 FEYWTDS(1) SFTYWTN(9) SFTYWSY(5) SFVYWLP(1) SFTYHVP(3) NSFSYWE(2) DSFRYWA(1) FSFSYWT(1) LSFEYYI(1) TSFTYWY(1) ASFSYWS(1) STFHYWG(2) QTFSYWG(1) QTFLYWG(1) TTFHYWS(1) YTFSYWG(4) QTFLYWV(1) MTFEYWP(1) DTFTYWY(1) TTFTYWP(1) TVFTYWT(3) SQSFHYW(1) DSSFMYW(1) YSSFTYW(1) ESTFYYW(1) NETFWYY(1) SDTFWYF(1) DSPFMYW(1) QPAFMYW(1) EAWFSYW(1) [ST]-F-[Tx]-Y-W 30 Phage display (common panning) 3 PMID:12044970 Murine microglial cell line EOC 20 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 1087 QSXAAEGE(3) YPRATTAT(1) DPRSATMT(1) DPRTAAMA(1) DPRIATMS(1) DPRATTST(1) DPRGAAIS(1) DPRSSAMT(1) DPRAVSGT(1) DPRATTTT(1) DPRAATST(1) DPRATPGS(1) DPRINAST(1) DPRSATVS(1) LSXAAEGE(5) VPPSPSNT(1) DPR 16 Phage display (subtractive panning) 3 PMID:11398171 LNCaP prostate carcinoma cells Not determined. Not determined. Not determined. f8-8mer phage display library (X8) The starting library was initially depleted by one round of panning with a primary prostate epithelial cell monolayer followed by three rounds of biopanning with LNCaP cell monolayers. 1088 AGQGSQ(10)[0.49] LLVPLR(6)[0.53] VYMSPF(2)[0.54] FAYLLG(1)[0.66] VTEMRH(1)[0.30] DHKMRH(1)[0.07] SLHHAS(1)[0.31] VKCSPF(1)[0.42] 8 Phage display (common panning) 4 PMID:12440521 Fibroblast growth factor receptor 1, FGFR-1 Not determined. Not determined. Not determined. f3-6mer phage display library (X6) ELISA Absorbance at 450 nm was measured. Data shown were reproduced from the graph. To retain the natural conformation of FGFR1 during screening, we expressed recombinant FGFR1 on the surface of Sf9 insect cells. 1089 CPVDFDFLC CPRDFEFLC CPADFEFLC 3 Phage display (common panning) 2 PMID:12446478 Anti-PSA monoclonal antibody 5E4 PSA-α2M complexes Not determined. Not determined. CX7C+CX8C+CX10C+CX3CX3CX3C+CX3CX4CX2C phage display library pool 1090 CPSVDGGWTC CKSMDGGWTC CHSACSKHCFVHC 3 Phage display (common panning) 3 PMID:12446478 Anti-PSA monoclonal antibody 5E4 and H117 PSA-α2M complexes Not determined. Not determined. CX7C+CX8C+CX10C+CX3CX3CX3C+CX3CX4CX2C phage display library pool The primary library was panned for two rounds using MAb 5E4, after which the eluate was amplified and panned for one round with MAb H117. 1091 CTWHWSPEEC 1 Phage display (common panning) 2 PMID:12446478 Anti-PSA monoclonal antibody H117 PSA-α2M complexes Not determined. Not determined. CX7C+CX8C+CX10C+CX3CX3CX3C+CX3CX4CX2C phage display library pool 1092 CPSVDGGWTC CHSACSKHCFVYC CHSACSKHCFVHC 3 Phage display (common panning) 3 PMID:12446478 Anti-PSA monoclonal antibody 5E4 and H117 PSA-α2M complexes Not determined. Not determined. CX7C+CX8C+CX10C+CX3CX3CX3C+CX3CX4CX2C phage display library pool The primary library was panned for two rounds using MAb H117, after which the eluate was amplified and panned for one round with MAb 5E4. 1093 CRPWGSSRC CKSWGSSRC CNLYKVGC CHPYKVGC 4 Phage display (common panning) 3 PMID:12446478 Anti-PSA monoclonal antibody 5A10 and 4G10 PSA-α2M complexes Not determined. Not determined. CX7C+CX8C+CX10C+CX3CX3CX3C+CX3CX4CX2C phage display library pool The primary library was panned for two rounds using MAb 5A10, after which the eluate was amplified and panned for one round with MAb 4G10. 1094 CSVYPFWHC 1 Phage display (common panning) 2 PMID:12446478 Anti-PSA monoclonal antibody 4G10 PSA-α2M complexes Not determined. Not determined. CX7C+CX8C+CX10C+CX3CX3CX3C+CX3CX4CX2C phage display library pool 1095 CRSYPFWMC CVSYPFWKC CAVFPFWRC CVWWWGC CAPWWGC 5 Phage display (common panning) 3 PMID:12446478 Anti-PSA monoclonal antibody 5A10 and 4G10 PSA-α2M complexes Not determined. Not determined. CX7C+CX8C+CX10C+CX3CX3CX3C+CX3CX4CX2C phage display library pool The primary library was panned for two rounds using MAb 4G10, after which the eluate was amplified and panned for one round with MAb 5A10. 1096 CSGIAPWLC CGPGIDSWVC CGPGIRSWVC CDYMPLVDNC 4 Phage display (common panning) 2 PMID:12446478 Anti-PSA monoclonal antibody H50 PSA-α2M complexes Not determined. Not determined. CX7C+CX8C+CX10C+CX3CX3CX3C+CX3CX4CX2C phage display library pool 1097 CEGIAVWLC CTRYSGYWVC CTQMNGYWIC CLYDHLC CILLYNDCC 5 Phage display (common panning) 3 PMID:12446478 Anti-PSA monoclonal antibody H50 and 9C5 PSA-α2M complexes Not determined. Not determined. CX7C+CX8C+CX10C+CX3CX3CX3C+CX3CX4CX2C phage display library pool The primary library was panned for two rounds using MAb H50, after which the eluate was amplified and panned for one round with MAb 9C5. 1098 CFFGNWNFC 1 Phage display (common panning) 2 PMID:12446478 Anti-PSA monoclonal antibody 9C5 PSA-α2M complexes Not determined. Not determined. CX7C+CX8C+CX10C+CX3CX3CX3C+CX3CX4CX2C phage display library pool 1099 CWNWNLHISC CYFKNWNFC CVRSCISDCELRC 3 Phage display (common panning) 3 PMID:12446478 Anti-PSA monoclonal antibody H50 and 9C5 PSA-α2M complexes Not determined. Not determined. CX7C+CX8C+CX10C+CX3CX3CX3C+CX3CX4CX2C phage display library pool The primary library was panned for two rounds using MAb 9C5, after which the eluate was amplified and panned for one round with MAb H50. 1100 CWSFFSNIC(26) CWPFWGPWC(4) CRPFWGPWC(4) CQPWHWPPC(2) CWSFFKLYC(2) CNELRSGLC(1) CNSVRVYSC(1) CDILHYSKC(1) 8 Phage display (common panning) 3 PMID:12436474 Capsid protein Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 65% phage clones carried the fusion peptide C-WSFFSNI-C. The N- and C-terminal residues represent constrained cysteine residues. 20% phage clones exhibited the sequence motif C-W/RPFWGPW-C.