951 YLHQPGRIDCTARPLEKCSR(7) KSEGKAEAVWAFKAHGSAVS(3) VGQTKPKVEKLGESTSMRLL(2) GSESTAERKSPDTPQLVEKL(1) MAARWVQPMPFSKWTESLRG(1) 5 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody PAb122 Cellular tumor antigen p53 Not determined. Not determined. X20 fAFF1 phage display library First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 952 TTRSPRLPIELLFAG(4) LHTPKPPWSVTSFVP(3) VSRIPISYLLQYSNR(2) TLTNVRLSLDSWLKR(2) SYPKLPWSFIKQLNK(1) SLSTEPRLPWSFFFS(1) MQISLSPRIPWSKIL(1) PTVLIRKSLPWSLIL(1) TLWTSTRFPIPWSLL(1) PQTPWGYLWDTSRFR(1) SMLEPRSKISWKSIL(1) NLSKKLSWDRVLTMM(1) HAKLNWDYILNTANV(1) LSLPKKLAWADILPS(1) LSQANRALLSWDKIL(1) LFSSKVPISYLFDIT(1) NLKLPLTFLDLSRLY(1) RIPLFNLSDLMGMQS(1) TTQRLPMRIPEVFSF(1) LPAKLPMESWLRSHP(1) KIPAQLWMQFAKSTN(1) PYWSNRAKLPMQWYQ(1) LKSPLTWDQVLLQSS(1) TYPPRLSTEFLFGNV(1) VTSALHQRLNISHIF(1) LSSVKLTLRQLFPYV(1) MPRGRLKVSEIFSTR(1) LNPGYLRKLNVLSIL(1) QWLLMKANPLDLLYR(1) LPMMKRDLRNLLNIV(1) STVTRMDLLKLLSSM(1) PVKLHMDILEVLQSH(1) PTPKQKINLQDWLLL(1) KLSIWDVLFDQAFAG(1) ATQHRKYLTLDDWLR(1) SPPWVFREHVYNLLP(1) DVPLNWSTFFSPSTR(1) AYHWSQSPLAIERIL(1) RHHTLIDLMSLLALQ(1) RLLSWSQILTLSDST(1) YPLPIMRLLDSFSSK(1) LLNMSRVLEYHTPSK(1) MQQTNSWMKWKTQAF(1) AFPHAPMSIDHIFFT(1) [KR]-[LI]-x-W-x(2)-I-L 44 Phage display (common panning) 3-5 PMID:7540176 Protein S100-B F-actin-capping protein subunit alpha-1 Not determined. Not determined. X15 M13 phage display library S-100b binding bacteriophages were selected by Ca(2+)-dependent affinity chromatography, and the sequence of the random peptide insert contained in 51 clones was determined. 953 EHDFHHIREWGNHWKNFLAVM[150 ± 30] YEKPWQNLWDWGAEAFKDLLDK[240 ± 80] LSFDWSELRRWGTWAAAEVFEL[150 ± 40] ELWRNLRLWGYCMNLSNMPL[930 ± 130] EFSLFKDIYRWGNWAAGFYYGV[NT] SFCLFDSIFDWGARGAEWHVG[NT] SCNMWRNIYSWGATFPQDHIFF[NT] DMDLFQHLSCWGTDFADMMFDE[NT] SCAWFLHLRQWGNSNWTYIFGV[NT] MHFGWYHLSEWGKIAALIGFEM[NT] ETGWFHNLTAWGEESVYALHIM[NT] SPGLFDNLKTWGTRMEHFMSLS[NT] QSFSMMSRWGQEFHNMLINV[NT] TALGWVHMHEWGEEFSKFPWGN[NT] QDYWTNMLIWGSMLLWSLFGADP[NT] HDYWQSMNHWGGVYGGCVPMCK[NT] YEEMWQWVFKAGQEWPWSYFPM[NT] QIPFQIQAYWLDVCCQEVTDDL[NT] IDQMWQWDPRWGXXXEEIDIHD[NT] SAFEYIQLTVSGWLPHLMGLF[NT] EKFEILCINYQGWISDLCEAIA[NT] WHDEDVLFRNLGEATLWLAEYY[NT] DWECMYDFCWRGXCVALNSTG[NT] DQTYKFEYNMGKDVTSLVCYW[NT] DNWIDSLVCIVGTGYGSLAAYY[NT] [WF]-x-[LIM]-x-W 25 Phage display (common panning) PMID:7542403 Antigen-presenting glycoprotein CD1d1 Not determined. Not determined. Not determined. X11GX12 phage display library Binding assay Dissociation constants (nM) for unlabeled peptides were determined from the molar concentration giving 50% inhibition of 125I-p99 binding to mCD1 in a competitive binding assay. NT denotes not tested. The recombinant soluble CD1-beta2 microglobulin complexes (mCD1) expressed in Drosophila melanogaster SC2 cells were used in the panning. The mCD1 was also engineered with a COOH-terminal hemagglutinin (HA) tag (YPYDVPDYAS), an epitope derived from the influenza HA protein. In this way, mCDl-phage complexes could be identified with a HA tag-specific antibody. Forty-seven different clones were selected by mCD1 binding. However, only 25 sequences shows a well-defined core motif were given in the original paper. 954 AGELSMQEWQRFRDLSLGRS RTPPLAETPEWQYQRYLFLL 2 Phage display (common panning) 3 PMID:7543411 Anti-keratin 19 monoclonal antibody BM19.21 Keratin, type I cytoskeletal 19 Not determined. Not determined. X20 fAFF1 phage display library At least 10 individual phage clones were sequenced. Only unique sequences were given. 955 MWDERWEQEELLGRRLLEAP 1 Phage display (common panning) 3 PMID:7543411 Anti-keratin 19 monoclonal antibody LE64 Keratin, type I cytoskeletal 19 Not determined. Not determined. X20 fAFF1 phage display library At least 10 individual phage clones were sequenced. Only unique sequences were given. 956 GESMAERSMAQERLLTYFMV GGGERSLVQESLLWSASSYA EADRAADQEAQMWTTKAVML ESDRDWGQECSNWYADCRYP [ED]-R-x(3)-Q-E 4 Phage display (common panning) 3 PMID:7543411 Anti-keratin 19 monoclonal antibody KM4.62 Keratin, type I cytoskeletal 19 Not determined. Not determined. X20 fAFF1 phage display library At least 10 individual phage clones were sequenced. Only unique sequences were given. 957 NEWGSKDELEFYSHLVSSLR EQAELTRTMQQLFRGTQVRS GEVMHQGDWGAENFYLTLSA AREGKDDQLFLYQSLQRYWR EAWDKRDEQTIYESLSWLSA LGEDKERHRESWLVYKTLTW GENAEALEAQVYYQSLRDFW QGWDHLYGPYYFTAADEGNL 8 Phage display (common panning) 3 PMID:7543411 Anti-keratin 19 monoclonal antibody LP2K Keratin, type I cytoskeletal 19 Not determined. Not determined. X20 fAFF1 phage display library At least 10 individual phage clones were sequenced. Only unique sequences were given. 958 AIVIENYYTLCC HLQIYYTSLA 2 Phage display (common panning) 3 PMID:7543411 Anti-keratin 19 monoclonal antibody LP2K Keratin, type I cytoskeletal 19 Not determined. Not determined. X12 fAFF1 phage display library At least 10 individual phage clones were sequenced. Only unique sequences were given. 959 SRGDYLSWEAYKTLMGASSS VSWEAYGEGAAGFFRLAEAL SWEAY 2 Phage display (common panning) 3 PMID:7543411 Anti-keratin 19 monoclonal antibody BA16 Keratin, type I cytoskeletal 19 Not determined. Not determined. X20 fAFF1 phage display library At least 10 individual phage clones were sequenced. Only unique sequences were given. 960 CRQNWGLEGC(26)[9548] CVDVVSASTC(4)[5720] CVRSESMLHC(3)[176] CRDPRAQDLC(1)[89] CLRSGIQNDC(1)[79] CAHLRAGRQC(1)[76] CSTMPPFASC(1)[64] CMDRGISQAC(1)[64] CLSAMVSGRC(1)[46] CPNSSGIRHC(1)[68] CWSLSTSIDC(1)[46] CSGGGFGSYC(1)[49] 12 Phage display (common panning) 7 PMID:7559473 MAR DNA Affinity Column DNA-binding protein SATB1 Not determined. Not determined. CX9 phage display library Binding assay The binding of selected phage clones to MAR was determined by a filter binding assay. The level of binding was quantitated by counting each spot in a liquid scintillation counter. Phage Selection were combined procedures for biopanning and methods for purifying DNA-binding proteins. One predominant cyclic peptide CRQNWGLEGC selected by a MAR-affinity column showed 50% identity with a segment in SATB1 (amino acids 355-363). 961 KPWYVSRV GKPWWASR EKPWWAVR GKPWYAGR KPSNVSRV YYCSPWCD VPWYKQST APWYRVSP LPWYLYPS 9 Phage display (common panning) 4 PMID:8170958 Purified IgM lamda receptor of the human Burkitt lymphoma cell line SUP-B8 Not determined. Not determined. Not determined. 8-mer ASASA-pIII phage display library All the peptides bound to the SUP-B8 immunoglobulin specifically. Their binding could be inhibited with anti-Id antibodies but not with anti-IgM or anti-lamda light-chain antisera. When conjugated to form dimers or tetramers, they induced cell death by apoptosis in vitro with an IC5o between 40 and 200 nM. This effect was associated with specific stimulation of intracellular protein tyrosine phosphorylation. 962 KPWWVSRV[0.97] KPWYVGRP[NT] KPWWVTRV[NT] KPWWVVRL[NT] GKPIWAGR[NT] QKPIWVTR[NT] DWAVWNRR[NT] NWAVWTKR[NT] NWGMWSKR[NT] YVFEDLFR[NT] MPEDFYRR[NT] SEPVDHGL[NT] VDPVDHGL[NT] VPIDHGT[NT] 14 Phage display (common panning) 4 PMID:8170958 Purified IgM lamda receptor of the human Burkitt lymphoma cell line SUP-B8 Not determined. Not determined. Not determined. 8-mer P4-pIll and 8-mer P6-pIII phage display library ELISA Biotinylated peptide monomer (0.4 μg per well) was immobilized on a microtiter well coated with 1 μg of streptavidin per well. SUP-B8 immunoglobulin or control immunoglobulin (0.25 μg) was added. Optical density values, OD (405-490 nm), were measured. Data shown were reproduced from the graph and expressed as means of duplicate samples ± SD. All the peptides bound to the SUP-B8 immunoglobulin specifically. Their binding could be inhibited with anti-Id antibodies but not with anti-IgM or anti-lamda light-chain antisera. When conjugated to form dimers or tetramers, they induced cell death by apoptosis in vitro with an IC5o between 40 and 200 nM. This effect was associated with specific stimulation of intracellular protein tyrosine phosphorylation. 963 KNGPWYAYTGRD FGILTEEMYRRW LRYTQEEMYRRW HYVHEDLYRRVK VTGYTMDVLYRR 5 Phage display (common panning) 4 PMID:8170958 Purified IgM lamda receptor of the human Burkitt lymphoma cell line SUP-B8 Not determined. Not determined. Not determined. 12-mer GG-pIII phage display library All the peptides bound to the SUP-B8 immunoglobulin specifically. Their binding could be inhibited with anti-Id antibodies but not with anti-IgM or anti-lamda light-chain antisera. When conjugated to form dimers or tetramers, they induced cell death by apoptosis in vitro with an IC5o between 40 and 200 nM. This effect was associated with specific stimulation of intracellular protein tyrosine phosphorylation. 964 PQVRPI(17) WIVNSP(1) PRVQIL(1) 3 Phage display (common panning) 3 PMID:7622517 Anti-p22phox monoclonal antibody 44.1 Cytochrome b-245 light chain Not determined. Not determined. f3-6mer phage display library (X6) Western blotting demonstrated specific binding of the antibody to the selected phage peptides. 965 HDQPMVLPI(8) EGRFGGGQV(3) AQPQVRPIG(1) NMPQVRPID(1) DRPRVKPK(1) GTPEVRPV(1) QPVVRPIDA(1) KEKPMVRPL(1) KPMVRPLVN(1) RPQVIPISV(1) KPMVIPIHS(1) QQPRVLPLD(1) MTPEVRPID(1) ENRPKVSPI(1) NKPRVMPLD(1) TGPEVRPLQ(1) RPMVHPLTP(1) DRPWVRPIV(1) TTRPEVRPI(1) HHHPMIPPI(1) EDRPMVRV(1) 21 Phage display (common panning) 3 PMID:7622517 Anti-p22phox monoclonal antibody 44.1 Cytochrome b-245 light chain Not determined. Not determined. X9 phage display library Western blotting demonstrated specific binding of the antibody to the selected phage peptides. 966 PKGAYD(9) FKRGVD(5) LREGVD(1) PRPAVD(1) LRRGID(1) 5 Phage display (common panning) 3 PMID:7622517 Anti-gp91phox monoclonal antibody 54.1 Cytochrome b-245 heavy chain Not determined. Not determined. f3-6mer phage display library (X6) Western blotting demonstrated specific binding of the antibody to the selected phage peptides. 967 GRPPKAGVD(3) GGNLKVGVD(1) KFYPKQSVD(1) QKLSKLAVD(1) NRLDKAAVD(1) IARNAVDGP(1) KAVEKSAVD(1) DAMPAEGVD(1) KTILKQGVD(1) SGPPKPALD(1) SSIIKDGVD(1) EMPLKRGVD(1) ERMPAHGVD(1) SRPPKGAID(1) KIPPRSAVD(1) SKTPKEGLD(1) 16 Phage display (common panning) 3 PMID:7622517 Anti-gp91phox monoclonal antibody 54.1 Cytochrome b-245 heavy chain Not determined. Not determined. X9 phage display library Western blotting demonstrated specific binding of the antibody to the selected phage peptides. 968 PWWWVSWVDAGGGSLALPTQPSD WGWPTWWGWTGGDARHPSAPEAH WWDPDIWFGWGGAHPPNLIQPIS GWQSGWEWWIGGGNWTSNTTH PYWMFYGFDWRGGFPPSHQIMDQ WMQSWYYHWGGGETFPIRRDSGG 6 Phage display (common panning) 3 PMID:7737512 96-well immunoassay plate (polystyrene, PS) Not determined. Not determined. Not determined. TSAR-12 phage display library The plastic-binding phage bind to both unblocked plastic (polystyrene, PS and polyvinyl chloride, PVC) and plastic blocked with bovine serum albumin (BSA) but require non-ionic detergent to bind to plastic blocked with milk. 969 EIHGNLYNWSPLLGYSYFPGISPKHISGEVPPRPSTKV YTGWETWYSFDPFTHYGGPGSRFDFVHDKSEDPIDRSY QDLDHWSYWSMYSTYPTSPGLVPYSWGYGSPNSHTDKL IYYPFFVWGNYANGGLLSPGHVYSSNFIPLYMQREVSP SSRLAYDHYFPSWRSYIFPGSNSSYYNNSWPTITMETN RPYLYDPNEWHRYYSYLLPGHSYNVQSWPDGLGKSASR LGFSGWYWQGLYGLGSHDPGFIHEQSPAEVAMEDTEQS QTLIDFHDLHYWGAYYGWPGIYDEASGSQAVRHNMTHT DSWPLRIYSGLSNYYHYFPGSLVYNMMYPSLSRLPKGD HHGAMNRYYTWLWDNSRFPGRSYLLSAPATQPEASISQ TYDYTYDWSGLFWSPFTHPGAHMTTHSPWAGHKPHAET 11 Phage display (common panning) 3 PMID:7737512 96-well immunoassay plate (polystyrene, PS) Not determined. Not determined. Not determined. TSAR-9 phage display library (X18PGX18) The plastic-binding phage bind to both unblocked plastic (polystyrene, PS and polyvinyl chloride, PVC) and plastic blocked with bovine serum albumin (BSA) but require non-ionic detergent to bind to plastic blocked with milk. 970 RPHQVV(1) LPFNMT(1) VPRAIS(1) EAFGMR(1) RPQTLA(1) 5 Phage display (common panning) 3 PMID:7896777 Stromelysin-1, SL-1 Not determined. Not determined. Not determined. X6 fAFF1-tether C phage display library In the first round, 2E11 TU (about 1000 equivalents of each clone) were treated with stromelysin. The output was not titered before the next round of screening, thus variable amounts of phage were used as input for the succeeding round. In later rounds of screening, the protease concentration was reduced. After each round, 12 clones were analyzed using the phage proteolysis assay. 971 GPMAMT(1) APQATV(1) APSAMS(1) 3 Phage display (common panning) 4 PMID:7896777 Stromelysin-1, SL-1 Not determined. Not determined. Not determined. X6 fAFF1-tether C phage display library In the first round, 2E11 TU (about 1000 equivalents of each clone) were treated with stromelysin. The output was not titered before the next round of screening, thus variable amounts of phage were used as input for the succeeding round. In later rounds of screening, the protease concentration was reduced. After each round, 12 clones were analyzed using the phage proteolysis assay. 972 IPFEQR(1) 1 Phage display (common panning) 5 PMID:7896777 Stromelysin-1, SL-1 Not determined. Not determined. Not determined. X6 fAFF1-tether C phage display library In the first round, 2E11 TU (about 1000 equivalents of each clone) were treated with stromelysin. The output was not titered before the next round of screening, thus variable amounts of phage were used as input for the succeeding round. In later rounds of screening, the protease concentration was reduced. After each round, 12 clones were analyzed using the phage proteolysis assay. 973 RPHQVV(1) PFNSVS(1) KAFELT(1) SLYELR(1) 4 Phage display (common panning) 6 PMID:7896777 Stromelysin-1, SL-1 Not determined. Not determined. Not determined. X6 fAFF1-tether C phage display library In the first round, 2E11 TU (about 1000 equivalents of each clone) were treated with stromelysin. The output was not titered before the next round of screening, thus variable amounts of phage were used as input for the succeeding round. In later rounds of screening, the protease concentration was reduced. After each round, 12 clones were analyzed using the phage proteolysis assay. 974 VPMSMS(2) GANFFE(1) NDYPAF(1) GVDFLE(1) FNEFSN(1) RPESMT(1) APLQST(1) IPSEMR(1) KPHALS(1) APLESI(1) APLEAV(1) APLTLS(1) EPMSLS(1) PKPLAL(1) PMAMMG(1) APFRLE(1) VPLETR(1) GPYSLT(1) VPMEIK(1) AWLLRW(1) EPYELY(1) TPFAFA(1) VPYELA(1) EPLGLV(1) APYEVM(1) MLLAST(1) GPWESK(1) PMEMVE(1) VPLELK(1) PMAMVA(1) 30 Phage display (common panning) 4 PMID:7896777 Stromelysin-1, SL-1 Not determined. Not determined. Not determined. X6 fAFF1-tether C phage display library In the first round, 2E10 TU (about 100 equivalents of each clone) were treated with stromelysin at 1 μg/ml. The output phage were titered before the next round, and a constant input was used for the succeeding round. 975 EPMSLS(2) MPLSLT(1) PLEIRA(1) HPMDVK(1) MLAELR(1) ILELQG(1) APMQLE(1) MILDLK(1) EPMDLI(1) VMELQG(1) YAMELR(1) SIQALT(1) RPLQIV(1) PANIKG(1) VPMNMT(1) AKKARM(1) PMTLAG(1) IPLPLT(1) KKVRRV(1) RRVRKV(1) GPLGLH(1) 21 Phage display (common panning) 3 PMID:7896777 Matrilysin Not determined. Not determined. Not determined. X6 fAFF1-tether C phage display library