926 DLLFEL DLLWDL DLLYDL DLLYHL DLLWLV DLFWEL DLMFIM D-L(2)-x(2)-L 7 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE14 Proenkephalin-A Not determined. Not determined. f3-6mer phage display library (X6) Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 927 DLLWSL DLLYEL DLLWHL DLLYDL DLMWAL DLLWES DLLFYY DWLFDR DRLWHY DWLWHY DFMIEL 11 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE15 Proenkephalin-A Not determined. Not determined. f3-6mer phage display library (X6) Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 928 GGWMRGHTHLST TDGGGWMVGYRM KFQSPIAWGFVR GMTILGGRMGFR 4 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE1 Proenkephalin-A Not determined. Not determined. X12 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 929 GPEEEAGGGGFMRGASRMTQ KLAGGWMQGHTVVPSREAKA GGFMAGIREKGAGREWNALT TVGGWMWGQGLKMAGQGRQS MKPSCAHADHGALGWMRGRL TEDRDLPEVLGGWMRGRYPN EGWMRGYRGFLQSQNLESGW G(2)-W-M-R-G 7 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE1 Proenkephalin-A Not determined. Not determined. X20 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 930 IWTWEPSKRYQL 1 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE2 Proenkephalin-A Not determined. Not determined. X12 fAFF1 phage display library For antibody PE2, the amplified library after the first round was subjected to two further rounds of antibody panning. In each round, 10 ml of library was panned on Petri dishes coated with 10 mg/ml PE2 and subsequently blocked with TBST buffer containing 5% (w/v) low-fat milk powder, for one hour. 931 YGSWAS(2)[NT] YGGLGL(1)[NT] YGSLVL(1)[NT] YGGLGI(1)[NT] YGSLVQ(1)[NT] YGGLGR(1)[NT] YGSLVR(1)[NT] YGGLNV(1)[NT] YGSLAD(1)[NT] YGGLRA(1)[NT] YGSLLS(1)[NT] YGGLEM(1)[NT] YGSLNG(1)[NT] YGSLYE(1)[NT] YGGIAS(1)[NT] YGGIAV(1)[NT] YGGIRP(1)[NT] YGSWQA(1)[NT] YGGWAG(1)[7.8] YGGWGP(1)[NT] YGSFLH(1)[NT] YGGWSS(1)[NT] YGGMKV(1)[NT] YGGFPD(1)[2.3] YGALGG(1)[NT] YGALSW(1)[NT] YGALDT(1)[NT] YGALEL(1)[NT] YGAIGF(1)[NT] YGAWTR(1)[NT] YGWWGL(1)[NT] YGLWQS(1)[NT] YGWWLT(1)[NT] YGFWGM(1)[0.35] YGWLAT(1)[NT] YGKWSG(1)[NT] YGWANK(1)[NT] YGPFWS(1)[1.9] YGEFVL(1)[NT] YGNWTY(1)[7.8] YGDFAF(1)[NT] YGNFAD(1)[NT] YGNFPA(1)[NT] YAWGWG(1)[NT] YAGFAQ(1)[8.3] YGTFIL(1)[NT] YGTWST(1)[NT] YSMFKE(1)[NT] YGVWAS(1)[NT] YGVWWR(1)[NT] 52 Phage display (common panning) 3 PMID:2201029 Anti-beta-endorphin monoclonal antibody 3-E7 Beta-endorphin Not determined. Not determined. X6 fAFF1 phage display library Radioimmunoassay A solution radioimmunoassay (RIA) was used to estimate the binding affinities of peptides for mAb 3-E7. Six hexapeptides with sequences corresponding to those of antibody-selected phage were chemically synthesized and assayed for binding to 3-E7. Each Kd (μM) value is the antilogarithm of the mean of log Kd from three to six independent determinations with standard deviations of <0.25 logarithm units in all cases. NT denotes not tested. Compared with 7.1 nM for a known high-affinity ligand (YGGFL), the binding affinities of six chemically synthesized hexapeptides from this set range from 0.35uM (YGFWGM) to 8.3uM (YAGFAQ). 932 WNVHGIWQE(2) DWDTRGLWVA(2) WWTDTGLW(1) WWTDDGLW(1) WWDTRGLWVWTI(1) FWGNDGIWLESG(1) DWDQFGLWRGAA(1) RWDDNGLWVVVL(1) CWSMHGLWLC(1) SGMWSHYGIWMG(1) GGRWDQAGLWVA(1) KLWSEQGIWMGE(1) GCWDNTGIWVPC(1) DWDTRGLWVY(1) SLWDENGAWI(1) KWDDRGLWMH(1) QAWNERGLWT(1) QWDTRGLWVA(1) SWDTRGLWVE(1) SWGRDGLWIE(1) EWTDNGLWAL(1) SWDEKGLWSA(1) SWDSSGLWMD(1) W-x(3)-G-x-W 23 Phage display (common panning) 3-4 PMID:8693002 Interleukin-1 receptor type 1 Not determined. Not determined. Not determined. X8, X10 and X12 phage display library pool Extracellular domain of type I IL-1 receptor was fuse to Fc domain of IgG1. The IL-lR-Fc fusion was then used as target. These peptides blocked binding of IL-la to the type I IL-1 receptor with IC50 values of 45-140uM. They also blocked IL-1-driven responses in human and monkey cells; howver, they did not bind the human type II IL-1 receptor or the murine type I IL-1 receptor. 933 HITWDQLWNVMN 1 Phage display (common panning) 4 PMID:7545665 E-selectin Not determined. Not determined. Not determined. X12 fAFF1 phage display library The soluble extracellular domain of E-selectin was expressed in a phosphatidylinositol (PI)-glycan-linked form on Chinese hamster ovary cells and was released from the cell surface with PI-PLC. A monoclonal antibody, mAb179, recognizes a portion of the PI-glycan linkage signal sequence at the C terminus of the released receptor and was used to immobilize active receptor in microtiter wells for panning. 934 LRHSVI(5) RHSVIS(1) FRHSLL(1) WRHSVV(1) FKHSVV(1) LRHSIL(1) YRHSIV(1) RHS 7 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody PAb240 Cellular tumor antigen p53 Not determined. Not determined. f3-6mer phage display library (X6) First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 935 EGRHSVVHQGAA(2) FRHSVVDYGLYN(1) YSRHSVVYGDGQ(1) TLRHSIIFGGEW(1) AVRHSVIERTLS(1) GPGIRHSTVPAY(1) DRAPRIRHSVII(1) WIATTLMRHSVV(1) RHS 8 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody PAb240 Cellular tumor antigen p53 Not determined. Not determined. X12 fAFF1 phage display library First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 936 DGEQGFMINERHSVIPPWSS(4) DRHSVVTEDPGALDRRTHSF(1) DGALEERHSVLRRTEAEYQM(1) TQASAELERHSVVIRQPRFV(1) EPLEKCVRRHSMRDLSGVCP(1) TEVGNMASRVRHSVIEAANG(1) IELGEPKKVVVRHSIIGSHL(1) SGHHSEMRALKSSLRHSVIS(1) RHS 8 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody PAb240 Cellular tumor antigen p53 Not determined. Not determined. X20 fAFF1 phage display library First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 937 SDLYKL(6) SDLHKL(5) SDLNKL(1) S-D-L-x-K-L 3 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody DO-1 Cellular tumor antigen p53 Not determined. Not determined. f3-6mer phage display library (X6) First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 938 GWSDLHKLPPHT(3) DPQSDLGKLRTG(2) TGAPNSDLFKLS(2) YSDLWKLYRFDA(1) GGASDLDRLLSE(1) NMQSDAWKLLHN(1) WLASDLDKLVLG(1) NEVPWRGFQDPN(1) S-D-L-x-[KR]-L 8 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody DO-1 Cellular tumor antigen p53 Not determined. Not determined. X12 fAFF1 phage display library First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 939 SDLNKL(6) SDLHKL(4) SDLFKL(2) S-D-L-x-K-L 3 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody DO-7 Cellular tumor antigen p53 Not determined. Not determined. f3-6mer phage display library (X6) First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 940 GWSDLHKLPPHT(5) DPQSDLGKLRTG(3) GGASDLDRLLSE(2) YSDLWKLYRFDA(1) LEYSDLDRMTWP(1) S-D-L-x-[KR]-[LM] 5 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody DO-7 Cellular tumor antigen p53 Not determined. Not determined. X12 fAFF1 phage display library First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 941 SDLHKL(4) FSDLHK(2) FSDLNK(2) SDLWRL(1) SDLYKL(1) SDLGKL(1) FSDLYK(1) S-D-L-x-[KR] 7 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody Bp53-11 Cellular tumor antigen p53 Not determined. Not determined. f3-6mer phage display library (X6) First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 942 TGAPNSDLFKLS(5) DPQSDLGKLRTG(4) GWSDLHKLPPHT(1) GGASDLDRLLSE(1) NDTYSDLTRMIH(1) S-D-L-x-[KR]-[LM] 5 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody Bp53-11 Cellular tumor antigen p53 Not determined. Not determined. X12 fAFF1 phage display library First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 943 SDLHKL(13) 1 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody Bp53-12 Cellular tumor antigen p53 Not determined. Not determined. f3-6mer phage display library (X6) First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 944 YSDLWKLYRFDA(4) GWSDLHKLPPHT(4) HFSSRFSDLGKM(4) LTTHMSDLDRMH(1) S-D-L-x-[KR]-[LM] 4 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody Bp53-12 Cellular tumor antigen p53 Not determined. Not determined. X12 fAFF1 phage display library First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 945 DLWSWR(2) FDLWSF(2) DLWALL(1) DLWAFD(1) DLWSLL(1) DLWHFP(1) DLWRLV(1) DLWRFS(1) PDLWRF(1) LPDLWR(1) DLW 10 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody Bp53-19 Cellular tumor antigen p53 Not determined. Not determined. f3-6mer phage display library (X6) First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 946 SPTTVADLWAIM(3) FGDLWCFACNAV(1) LNDLWSISDPEW(1) ALTDLWSFLVLE(1) TTRLVDLWGFHL(1) TFHFSTDLWRLA(1) DYGRAMADLWQF(1) SNPNYPGDLWMF(1) RAHTGIMDLWGF(1) IARSAIHDLWSL(1) DLW 10 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody Bp53-19 Cellular tumor antigen p53 Not determined. Not determined. X12 fAFF1 phage display library First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 947 NKMWGK(5) RMNRPK(3) RFHAKF(3) RFWAKF(2) NRMWGK(1) 5 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody PAb421 Cellular tumor antigen p53 Not determined. Not determined. f3-6mer phage display library (X6) First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 948 KYLYSTTSSHVV(5) EWKGMNFIGSRP(4) TTSWHGRPDRNL(3) ITSHHGRPRALI(2) KEAASTSSADRR(2) LWVPSLFFSKLS(1) GDTWHGAYFYGR(1) KTNNTVCSKSVC(1) 8 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody PAb421 Cellular tumor antigen p53 Not determined. Not determined. X12 fAFF1 phage display library First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 949 GNGAVWEGKKLSTSSSLRVL(4) TKGKVDTGTIFGNAPRMAIS(2) GCGTSWQGELCKSIRLRYVP(2) APTGAKKGGTTSSLASHYRN(2) VHGEMVRKSFNSTTSRVPRT(1) WTESGRLTKRESSTNKNLGR(1) 6 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody PAb421 Cellular tumor antigen p53 Not determined. Not determined. X20 fAFF1 phage display library First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng. 950 KYSDSVSLTHRI(10) KLSQSTSPPVTG(1) GIKLGDSVSVWR(1) 3 Phage display (common panning) 3 PMID:7537340 Anti-P53 monoclonal antibody PAb122 Cellular tumor antigen p53 Not determined. Not determined. X12 fAFF1 phage display library First round, 10000ng of monoclonal antibody; second round, 100ng; third round, 10ng.