901 CIPRGDGWC(3) CTRGDGWFC(3) CERGDLRMC(2) CYRRGDGHC(1) CELRGDGWC(1) CVARGDGWC(1) CTRGDMQWC(1) CSRGDGWIC(1) CLRGDGFLC(1) CYRGDHLLC(1) CLRGDARFC(1) CTRGDGWPC(1) CGRGDRPQC(1) CKRGDGFWC(1) CRGDFSYMC(1) RGD 15 Phage display (common panning) 3 PMID:7507494 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. CX7C phage display library In the first and second panning, the coating concentration of the integrin was 5 microgram/well. In the third round of panning, the coating concentration of the integrin was 100 nanogram/well. 902 CELRGDGWC(2) CEYRGDGFC(1) CVARGDGWC(1) RGD 3 Phage display (common panning) 4 PMID:7507494 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. CX7C phage display library In the first and second panning, the coating concentration of the integrin was 5 microgram/well. In the third and fourth panning, the concentrations were 100 and 10 ng/well, respectively. 903 CIPRGDGWC(2) CVARGDGWC(1) CQTRGDGWC(1) CLFRGDGWC(1) CLSRGDGWC(1) CFRGDGFVC(1) CRGDGFGSC(1) RGD 7 Phage display (common panning) 5 PMID:7507494 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. CX7C phage display library In the first and second panning, the coating concentration of the integrin was 5 microgram/well. In the fifth round of panning, the coating concentration of the integrin was 10 ng/well. 904 CIPRGDGWC(1) CMTRGDGFC(1) CLFRGDGWC(1) CRGDGFGSC(1) RGD 4 Phage display (common panning) 5 PMID:7507494 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. CX7C phage display library In the first and second panning, the coating concentration of the integrin was 5 microgram/well. In the fifth round of panning, the coating concentration of the integrin was 1 ng/well. 905 CRRETAWAC(15) CASVNGHTC(4) CSTSDVGGC(3) CWANGRSHC(3) CLNTNLGFC(2) CFVNGRSFC(2) CVRLNSLAC(2) CEIVKSSSC(1) CPELFVESC(1) CGPCSGKSC(1) CRGAPRAWC(1) CFAGSLLVC(1) CNLTLSVSC(1) CFANGRHSC(1) CRFGSHVPC(1) CTLVPSRSC(1) CVLNGRMEC(1) CSRPSTFEC(1) CYVNGRVSC(1) CSVANSVVC(1) CWLNGRINC(1) CASFFAVQC(1) CVNVEYRNC(1) CMANGRLLC(1) CHVLASAFC(1) CLNGRGLMC(1) CVFSIAHEC(1) CLVASMTPC(1) CIGTFHHNC(1) CAFYQGLPC(1) CQNAFGYSC(1) CLGEFAFAC(1) 32 Phage display (common panning) 2-5 PMID:7507494 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. CX7C phage display library In the first and second panning, the coating concentration of the integrin was 5 microgram/well. NGR, and its variation, NGH represented alpha5/beta1 integrin binding motif other than RGD. Surprisingly, CRRETAWAC does not bear obvious resemblance to known integrin ligand sequences. However, synthetic cyclic peptide GACRRETAWACGA was a potent inhibitor of integrin alpha5/beta1-mediated cell attachment to fibronectin. This peptide is nearly specific for alpha5/beta1 integrin. CRRETAWAC appears to interact with the same or an overlapping binding site in integrin alpha5/beta1 as RGD. The importance of the disulfide bond was indicated by the fact that the activity of CRRETAWAC peptide was greatly diminished after reduction of the disulfide bond and alkylation of the cysteines. 906 ARLSRK(2) RLSRK(1) LRLSRK(1) GPLRSM(1) RQLRSM(1) LRSM(1) RGMTLS(1) LHMTLS(1) LVAVPT(1) PCSVHT(1) ALAGHS(1) QCGDVK(1) PVLCIT(1) ACCCLN(1) 14 Phage display (common panning) 4 PMID:7523009 Anti-ovarian carcinoma monoclonal antibody OV-TL3 Leukocyte surface antigen CD47 Not determined. Not determined. f3-6mer phage display library (X6) 907 ARLSRK(2) AKLSRK(1) ANLSRK(1) GPFRSM(1) RQLRSM(1) VSRHSM(1) RKLLRT(1) QCVSIM(1) SSPIVS(1) TEPIVS(1) TSLDRI(1) GAYHTV(1) ASCILS(1) SRYIGS(1) 14 Phage display (common panning) 4 PMID:7523009 Anti-ovarian carcinoma monoclonal antibody OV-TL16 Leukocyte surface antigen CD47 Not determined. Not determined. f3-6mer phage display library (X6) 908 SCRGDC(2) DTRGDW(2) CRGDCA(2) FMTRGD(1) CRGDCT(1) SRGDSF(1) NCRGDC(1) SRGDNT(1) FSRGDR(1) LNRGDG(1) WLGRGD(1) DRGDTY(1) RGDSFN(1) NGRIPD(1) TNGRGP(1) NGRSRF(1) TVVRGD(1) RGDAWL(1) RGDSLT(1) TMRGDV(1) VRGDGV(1) TRGDPA(1) RSRNGR(1) NGRNTV(1) RGD 24 Phage display (competitive panning) 3 PMID:7535098 Integrin alpha-V-beta-3, integrin αvβ3 Not determined. Not determined. Not determined. f3-6mer phage display library (X6) Phage were eluted with 0.1mL of 1mM GRGDSP peptide in incubation buffer. Integrin alpha-V beta-3 binds promiscuously to cell-adhesive proteins: vitronectin, fibronectin, and several others containing the RGD motif. 909 SCRGDC(2) SLPRGD(2) WMRGDV(1) GSRGDF(1) NSRGDF(1) FLTRGD(1) AFRGDS(1) VLFRGD(1) KSMRGD(1) GRGDGS(1) PVRRGD(1) MRGDRG(1) SRGDGI(1) RGDSYP(1) VLARGD(1) INSRGD(1) RGDGYV(1) RENRGD(1) GFRGDG(1) TIRGDQ(1) TWNHLS(1) RGD 21 Phage display (common panning) 3 PMID:7535098 Integrin alpha-V-beta-3, integrin αvβ3 Not determined. Not determined. Not determined. f3-6mer phage display library (X6) Phage were eluted with 0.1 M glycine, pH 2.0, containing 1 mg/mL BSA. Integrin alpha-V beta-3 binds promiscuously to cell-adhesive proteins: vitronectin, fibronectin, and several others containing the RGD motif. 910 TWRRGD(2) NGLRGD(2) TKRGDH(1) RRGDHI(1) RGDGSS(1) LQRGDW(1) ATRGDT(1) FRGDFS(1) VLRGDN(1) TRRGDT(1) QIARGD(1) FPVRGD(1) RGDFFS(1) QYLRGD(1) ARWRGD(1) DSARGD(1) RGDSLQ(1) SRLRGD(1) RGDRSL(1) RGDGWL(1) PTQRGD(1) RGDYMD(1) TRGDSL(1) RGDFAF(1) RGD 24 Phage display (competitive panning) 3 PMID:7535098 Integrin alpha-V-beta-3, integrin αvβ3 Not determined. Not determined. Not determined. f3-6mer phage display library (X6) Phage were eluted with low pH in the first round of screening, followed by elution with GRGDSP peptide in the second and third rounds. Integrin alpha-V beta-3 binds promiscuously to cell-adhesive proteins: vitronectin, fibronectin, and several others containing the RGD motif. 911 RGDMSLLGEFTSPYG(8) SRGDGGFIKVLHGSW(3) RGDAIVTFPGSFMLY(2) RRGDIGPRFESAIVD(2) PRGDAFPHMQSSASV(1) PEVIARGDVVFFLRP(1) DALSCRGDCVWPTRG(1) ARGDVFYEGSRGAWY(1) RGDAYAFGNAGVDLI(1) ACGSAGTCSPHLRRP(1) RGD 10 Phage display (competitive panning) 3 PMID:7535098 Integrin alpha-V-beta-3, integrin αvβ3 Not determined. Not determined. Not determined. f3-15mer phage display library (X15) Phage were eluted with 0.1mL of 1mM GRGDSP peptide in incubation buffer. Integrin alpha-V beta-3 binds promiscuously to cell-adhesive proteins: vitronectin, fibronectin, and several others containing the RGD motif. 912 RGDAIVTFPGSFMLY(4) GRGDRTDGSSGHVWG(2) GERGDGSFFAFRSPF(2) HAAFEPRGDVRHTLL(2) SRGDGGFIKVLHGSW(2) RGDMSLLGEFTSPYG(2) VRGDSLLFGVQAVLH(1) AGRGDSLGNYRNFNS(1) RGVRGDSFFLVMDAH(1) PRGDAFPHMQSSASV(1) SLRGDHRVRWVLTPH(1) DNSHWFRRISRGDAG(1) MWVFSRGDSSLFCCG(1) RALRGDRGWIVFWDP(1) RGD 14 Phage display (common panning) 3 PMID:7535098 Integrin alpha-V-beta-3, integrin αvβ3 Not determined. Not determined. Not determined. f3-15mer phage display library (X15) Phage were eluted with 0.1 M glycine, pH 2.0, containing 1 mg/mL BSA. Integrin alpha-V beta-3 binds promiscuously to cell-adhesive proteins: vitronectin, fibronectin, and several others containing the RGD motif. 913 RGDMSLLGEFTSPYG(10)[NT] DALSCRGDCVWPTRG(6)[100] RRGDIGPREFSAIVD(4)[NT] SPARGDLFRFMGAVH(2)[NT] PRDHIARRGDLAFQA(1)[NT] RGD 5 Phage display (competitive panning) 3 PMID:7535098 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. f3-15mer phage display library (X15) Binding assay IC50 (nM) values for the inhibition of binding of adhesive proteins to αvβ3 or α5βl integrins by synthetic peptides were measured using a solid-phase assay. Phage were eluted with 0.1mL of 1mM GRGDSP peptide in incubation buffer. 914 RRGDIGPREFSAIVD(5)[NT] ISRRGDLSGLSFSRL(4)[4000] RGDMSLLGEFTSPYG(4)[NT] DALSCRGDCVWPTRG(3)[100] RGVKMRRGDFSTIMD(1)[NT] SPARGDLFRFMGAVH(1)[NT] RVFRGDLGYRTPYIG(1)[NT] RGDVWTLWSVGDTRS(1)[NT] SEELLVESSAIRSRE(1)[NT] RGD 9 Phage display (common panning) 3 PMID:7535098 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. f3-15mer phage display library (X15) Binding assay IC50 (nM) values for the inhibition of binding of adhesive proteins to α5β1 integrins by synthetic peptides were measured using a solid-phase assay. Phage were eluted with 0.1 M glycine, pH 2.0, containing 1 mg/mL BSA. 915 RETADDLLSLLL VRYPSDLLLMIL STEPDLLWIIMG QDDILWEILAVD LGNNDDLLFFNL RGCGVDLLDCLL D-L(2)-x(2)-L(2) 6 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE14 Proenkephalin-A Not determined. Not determined. X12 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 916 HDDSDLLLTFLR RETADDLLSLLL EYTNDLLLRLLS STEPDLLWIIMS IDTDLLYVITQS TADFLYLITTWS TSDLGMYLFGSV L(2)-x(2)-L 7 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE15 Proenkephalin-A Not determined. Not determined. X12 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 917 RETADDLLSLLL EYTNDLLLRLLS D-L(2)-x(2)-L(2) 2 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE19 Proenkephalin-A Not determined. Not determined. X12 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 918 HDDSDLLLTFLR EYTNDLLLRLLS D-L(2)-x(2)-L 2 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE23 Proenkephalin-A Not determined. Not determined. X12 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 919 RETADDLLSLLL EYTNDLLLRLLS LDSDLQLFFLSS 3 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE25 Proenkephalin-A Not determined. Not determined. X12 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 920 GDVTWPQGEEWTEEHWVRWS DNGLAGRDYERDVRERVMKL DPRVWGRDVEPHVFYKYWRN QTSDSLGGKDDESWHTTAKL QEEFVHGMDRERVVWLLTSA G-x-D-x-E-x(2)-V 5 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE13 Proenkephalin-A Not determined. Not determined. X20 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 921 LGAENSGGQDRSDVTAARYG AGRMSQRGVDQVEWHLSRFY SVPAGADQVSFHTDRALGAV AGEDSGAHHRLRWETGRARW 4 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE20 Proenkephalin-A Not determined. Not determined. X20 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 922 SVPAGADQVSFHTDRALGAV ERTSGVDNPRYHATWTTRLR HPQGEDVQKSVVARWGDFLR VKGELRGVDERTRVMQQYG RDGKEGTQEAMYVMERYSIL AGEDSGAHHRLRWETGRARW TWKDRSTGGGMDMPGWHLQF 7 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE21 Proenkephalin-A Not determined. Not determined. X20 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 923 HPQGEDVQKSVVARWGDFLR GYGEDRERETWARWDRVTQA VAQGMAGQDKEREFRERLQN SVPAGADQVSFHTDRALGAV GLVLWGEDREEWHQSLQAGL THSAKTSLEGIDREAMVFIW KDMGRYGEDREHKVREWMRQ 7 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE22 Proenkephalin-A Not determined. Not determined. X20 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 924 MFGEDNYEFHFR MRMGYDNEKYHT G-x-D-N 2 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE24 Proenkephalin-A Not determined. Not determined. X12 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml). 925 SKLSPAMPGEDKERELAAKL VASIGGKDNEEAVWEHRGAV EPTGRDAEDWIYRAQGRFYL DLGVDIEMEVASRVRGGHSY VAQGMAGQDKEREFRERLQN WPYSCGQDNTSKVANRYARF HPQGEDVQKSVVARWGDFLR G-x-D-N 7 Phage display (common panning) 3 PMID:7536850 Anti-proenkephalin monoclonal antibody PE24 Proenkephalin-A Not determined. Not determined. X20 fAFF1 phage display library Three rounds of biopanning were carried out with decreased antibody concentrations (1 mg/ml and 0.1 mg/ml).