876 SWLIARPLPPLPPP(1)[NT] RALPPRPLPPLPPP(1)[NT] WSIRSRPLPPLPPP(1)[NT] YPFHGRPLPPLPPP(1)[NT] PWLLRRPLPPLPPP(1)[NT] SWLSVRPLPPLPPP(1)[NT] SWLGSRPLPPLPPP(1)[NT] PWWLDRPLPPLPPP(1)[1451.141] LTMAGRPLPPLPPP(1)[NT] DFMASRPLPPLPPP(1)[NT] DWYHSRPLPPLPPP(1)[NT] LSFSNRPLPPLPPP(1)[NT] MDLLRRPLPPLPPP(1)[NT] AAMVGRPLPPLPPP(1)[NT] RLLPPRPLPPLPPP(1)[NT] SWVACRPLPPLPPP(1)[NT] PWPVGRPLPPLPPP(1)[NT] 17 Phage display (common panning) 4 PMID:7479908 Lyn SH3 domain of tyrosine-protein kinase Lyn Not determined. Not determined. Not determined. N+5 class I phage display library Surface plasmon resonance (SPR) Binding of phage-displayed peptides to Src was measured by BIAcore technology. The amount of phage binding to immobilized SH3 domain is given as arbitrary resonance units (RU) and has been corrected for bulk refractive-index contributions of the unbound phage. Data shown were reproduced from the graph. NT denotes not tested. GST-Lyn SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 877 SALARRPLPPLPPP(2) PNLRTRPLPPLPPP(1) LPLSRRPLPPLPPP(1) SWWSRRPLPPLPPP(1) TFLFRRPLPPLPPP(1) THPARRPLPPLPPP(1) FPVAQRPLPPLPPP(1) PSPRSRPLPPLPPP(1) RSLSMRPLPPLPPP(1) VLLALRPLPPLPPP(1) SVLARRPLPPLPPP(1) NRAAKRPLPPLPPP(1) SSLAQRPLPPLPPP(1) TPLGRRPLPPLPPP(1) PNPSYRPLPPLPPP(1) SASARRPLPPLPPP(1) SWMARRPLPPLPPP(1) SPLARRPLPPLPPP(1) 18 Phage display (common panning) 4 PMID:7479908 Tyrosine-protein kinase Yes Not determined. Not determined. Not determined. N+5 class I phage display library GST-Yes SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 878 CLLTTRPLPPLPPP(7)[NT] CLNCFRPLPPLPPP(6)[984.609] CLNCSRPLPPLPPP(5)[NT] LLSGPRPLPPLPPP(4)[NT] CLNCLRPLPPLPPP(4)[NT] FKRPPRPLPPLPPP(1)[NT] FKRPFRPLPPLPPP(1)[NT] LVLPRRPLPPLPPP(1)[NT] FHRPNRPLPPLPPP(1)[NT] RRVPPRPLPPLPPP(1)[NT] WSRTKRPLPPLPPP(1)[NT] RVRPSRPLPPLPPP(1)[NT] RYLPWRPLPPLPPP(1)[NT] CCLTTRPLPPLPPP(1)[NT] RPFPRRPLPPLPPP(1)[NT] RVPIRRPLPPLPPP(1)[NT] ARLPPRPLPPLPPP(1)[NT] RYLSWRPLPPLPPP(1)[NT] 18 Phage display (common panning) 4 PMID:7479908 PI3K SH3 domain of PI3-kinase subunit p85-alpha Not determined. Not determined. Not determined. N+5 class I phage display library Surface plasmon resonance (SPR) Binding of phage-displayed peptides to Src was measured by BIAcore technology. The amount of phage binding to immobilized SH3 domain is given as arbitrary resonance units (RU) and has been corrected for bulk refractive-index contributions of the unbound phage. Data shown were reproduced from the graph. NT denotes not tested. GST-PI3K SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 879 RSLRPLPPPPVPSL(12)[352.868] RSLRPLPLPPVPSL(1)[139.410] RSLPLPPLPARPHP(1)[NT] RSFRPLPPLPTLLPS(1)[NT] RSLRPLPPLPTPPLH(1)[NT] 5 Phage display (common panning) 4 PMID:7479908 Src SH3 domain of Proto-oncogene tyrosine-protein kinase Src Not determined. Not determined. Not determined. C+5 class I phage display library Surface plasmon resonance (SPR) Binding of phage-displayed peptides to Src was measured by BIAcore technology. The amount of phage binding to immobilized SH3 domain is given as arbitrary resonance units (RU) and has been corrected for bulk refractive-index contributions of the unbound phage. Data shown were reproduced from the graph. NT denotes not tested. GST-Src SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 880 RSLRPLPPLPLPPLT(1) RSLRPLPPLPSAPRV(1) RSLRPLPPLPVLSEP(1) RSLRPLPPLPTSTQP(1) RSLRPLPPLPHLPDS(1) RSLRPLPPLPSYTSH(1) RSLRPLPPLPVATHP(1) RSLRPLPPLPSSLSR(1) RSLRPLPPLPAFHAR(1) RSFRPLPPLPTVASP(1) 10 Phage display (common panning) 4 PMID:7479908 Fyn SH3 domain of tyrosine-protein kinase Fyn Not determined. Not determined. Not determined. C+5 class I phage display library GST-Fyn SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 881 RSLRPLPPLPLPPRL(1)[NT] RSLRPLPPLPFPRYL(1)[NT] RSLRPLPPLPPSSAP(1)[NT] RSLRPLPPLPPRAPF(1)[NT] RSLRPLPPLPPRPPF(1)[377.387] RSLRPLPPLPSRPGP(1)[NT] RSLRPLPPLPPRPAY(1)[NT] 7 Phage display (common panning) 4 PMID:7479908 Lyn SH3 domain of tyrosine-protein kinase Lyn Not determined. Not determined. Not determined. C+5 class I phage display library Surface plasmon resonance (SPR) Binding of phage-displayed peptides to Src was measured by BIAcore technology. The amount of phage binding to immobilized SH3 domain is given as arbitrary resonance units (RU) and has been corrected for bulk refractive-index contributions of the unbound phage. Data shown were reproduced from the graph. NT denotes not tested. GST-Lyn SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 882 RSLRPLPPLPLPPRH(9)[477.953] RSLRPLPPLPLPPRT(1)[357.737] RSLRPLPPLPLPPRL(1)[NT] RSLRPLPPLPLPPPH(1)[NT] 4 Phage display (common panning) 4 PMID:7479908 PI3K SH3 domain of PI3-kinase subunit p85-alpha Not determined. Not determined. Not determined. C+5 class I phage display library Surface plasmon resonance (SPR) Binding of phage-displayed peptides to Src was measured by BIAcore technology. The amount of phage binding to immobilized SH3 domain is given as arbitrary resonance units (RU) and has been corrected for bulk refractive-index contributions of the unbound phage. Data shown were reproduced from the graph. NT denotes not tested. GST-PI3K SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 883 GAAPPLPPRNPPRT(2) GAAPPLPPRNKPRL(2) GAAPPLPPRNPPRL(1) GAAPPLPPRNKVRG(1) GAAPPLPPRNQTFR(1) GAAPPLPPRNRLHR(1) GAAPPLPPRNVPRL(1) GAAPPLPPRNRLHA(1) GAAPPLPPRNRVKL(1) GAAPPLPPRNRVRL(1) GAAPPLPPRNRVVL(1) GAAPPLPPRNIPRV(1) GAAPPLPPRNPPRK(1) GAAPPLPPRNKARL(1) 14 Phage display (common panning) 4 PMID:7479908 Src SH3 domain of Proto-oncogene tyrosine-protein kinase Src Not determined. Not determined. Not determined. C+5 class II phage display library GST-Src SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 884 GAAPPLPPRNRVRL(2) GAAPPLPPRNPPRV(1) GAAPPLPPRNPRPI(1) GAAPPLPPRNAVRL(1) GAAPPLPPRNRPHF(1) GAAPPLPPRNPKHR(1) GAAPPLPPRNPPRF(1) GAAPPLPPRNSRPL(1) GAAPPLPPRNPARL(1) GAAPPLPPRNTPRL(1) GAAPPLPPRNPRPL(1) GAAPPLPPRNPPRT(1) GAAPPLPPRNPPTV(1) 13 Phage display (common panning) 4 PMID:7479908 Fyn SH3 domain of tyrosine-protein kinase Fyn Not determined. Not determined. Not determined. C+5 class II phage display library GST-Fyn SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 885 GAAPPLPPRTYLLP(2) GAAPPLPPRTYGTT(1) GAAPPLPPRPPWMM(1) GAAPPLPPRTRYSL(1) GAAPPLPPRPPWMT(1) GAAPPLPPRTYWMP(1) GAAPPLPPRPTFMA(1) GAAPPLPPRPTWMT(1) GAAPPLPPRTYLMP(1) GAAPPLPPRPAWMA(1) GAAPPLPPRSYLLP(1) GAAPPLPPRTTPYT(1) GAAPPLPPRPSWMS(1) GAAPPLPPRPVTWI(1) GAAPPLPPRPSFMS(1) GAAPPLPPRPAFFS(1) GAAPPLPPRPSFLQ(1) 17 Phage display (common panning) 4 PMID:7479908 Lyn SH3 domain of tyrosine-protein kinase Lyn Not determined. Not determined. Not determined. C+5 class II phage display library GST-Lyn SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 886 GAAPPLPPRNPPRT(2) GAAPPLPPRNPPRI(2) GAAPPLPPRNRPRV(1) GAAPPLPPRNPKHR(1) GAAPPLPPRNPPRS(1) GAAPPLPPRNPKHW(1) GAAPPLPPRNPVRV(1) GAAPPLPPRNKARL(1) GAAPPLPPRNKHPP(1) GAAPPLPPRNPPRK(1) GAAPPLPPRNKRHL(1) GAAPPLPPRKHLSS(1) GAAPPLPPRNPPRV(1) GAAPPLPPRNKLRV(1) 14 Phage display (common panning) 4 PMID:7479908 Tyrosine-protein kinase Yes Not determined. Not determined. Not determined. C+5 class II phage display library GST-Yes SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 887 GAAPPLPPRPPRPA(2) GAAPPLPPRPPRPL(2) GAAPPLPPRPPLRS(1) GAAPPLPPRPPIRK(1) GAAPPLPPRPPLPF(1) GAAPPLPPRPPKPA(1) GAAPPLPPRPPRPP(1) GAAPPLPPRPPLRQ(1) GAAPPLPPRRALRL(1) GAAPPLPPRRLRVG(1) GAAPPLPPRPPRKP(1) GAAPPLPPRPPRTP(1) GAAPPLPPRPPRPS(1) GAAPPLPPRPPRPF(1) 14 Phage display (common panning) 4 PMID:7479908 PI3K SH3 domain of PI3-kinase subunit p85-alpha Not determined. Not determined. Not determined. C+5 class II phage display library GST-PI3K SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 888 CHPQGPPC(56)[8.1, 0.230] CHPQFPLC(4)[NT] CHPQFTLC(2)[NT] CHPQFSNC(1)[14.0, 0.400] CHPQFNLC(1)[NT] CHPQGDRC(1)[NT] CHPQFRHC(1)[NT] CHPQSGRC(1)[NT] HPQ 8 Phage display (common panning) 3 PMID:7492543 Streptavidin Biotin 1DF8,1LUQ,1MEP,1MK5,1N43,1N9M,1NDJ,1NQM,1STP,1SWD,1SWE,1SWG,1SWK,1SWN,1SWP,1SWR,1SWT,2F01,2GH7,2IZF,2IZG,2IZH,2IZI,2IZJ,2RTD,2RTE,2RTF,2RTG,2Y3F,3MG5, 1VWF,1VWG,1VWH,1VWI,1VWJ,1VWK,1VWL,1VWO,1VWP,1VWQ,1VWR,1SLE, CX6C M13 phage display library Surface plasmon resonance (SPR) KD (μM) and IC50 ((μM)) values were shown. Streptavidin-coated paramagnetic particles (Promega, Madison, WI) were used in the biopanning procedure. HPQGPPC showed the highest affinity, which was 3 orders of magnitude higher than those of the linear peptides such as FSHPQNT and HDHPQNL, identified previously. The structure of HPQGPPC-Streptavidin complex have been solved (1SLE). 889 CHPQFPC(28)[32.6, 0.930] CHPQFNC(4)[246.0, 7.0] CHPQVPC(4)[NT] CHPQVAC(3)[NT] CHPQFMC(2)[NT] CHPQFRC(2)[NT] CHPQNAC(1)[NT] CHPQVSC(1)[NT] CHPQFAC(1)[NT] CHPQVRC(1)[NT] CWHPQFC(1)[NT] HPQ 11 Phage display (common panning) 3 PMID:7492543 Streptavidin Biotin 1DF8,1LUQ,1MEP,1MK5,1N43,1N9M,1NDJ,1NQM,1STP,1SWD,1SWE,1SWG,1SWK,1SWN,1SWP,1SWR,1SWT,2F01,2GH7,2IZF,2IZG,2IZH,2IZI,2IZJ,2RTD,2RTE,2RTF,2RTG,2Y3F,3MG5, Not determined. CX5C M13 phage display library Surface plasmon resonance (SPR) KD (μM) and IC50 ((μM)) values were shown. Streptavidin-coated paramagnetic particles (Promega, Madison, WI) were used in the biopanning procedure. 890 CHPQFC(20)[16.0, 0.47] HPQ 1 Phage display (common panning) 3 PMID:7492543 Streptavidin Biotin 1DF8,1LUQ,1MEP,1MK5,1N43,1N9M,1NDJ,1NQM,1STP,1SWD,1SWE,1SWG,1SWK,1SWN,1SWP,1SWR,1SWT,2F01,2GH7,2IZF,2IZG,2IZH,2IZI,2IZJ,2RTD,2RTE,2RTF,2RTG,2Y3F,3MG5, 1SLD,1VWB,1VWC,1VWD,1VWE,1VWM,1VWN, CX4C M13 phage display library Surface plasmon resonance (SPR) KD (μM) and IC50 ((μM)) values were shown. Streptavidin-coated paramagnetic particles (Promega, Madison, WI) were used in the biopanning procedure. The structure of CHPQFC-Streptavidin complex have been solved (1SLD). 891 ISFENTWLWHPQFSS(5) LNHPMDNRLHVSTSP(4) SDDWWHDHPQNLRSS(3) PCHPQYRLCQRPLKQ(2) QPFLHPQGDERWYMI(2) MLWYSPHSFSHPQNT(1) SWWLSWHPQNTKELG(1) LCHPQFPRCNLFRKV(1) ALCCLSSPHPNGAIF(1) HPQ 9 Phage display (common panning) 2 PMID:2143033 Streptavidin Biotin 1DF8,1LUQ,1MEP,1MK5,1N43,1N9M,1NDJ,1NQM,1STP,1SWD,1SWE,1SWG,1SWK,1SWN,1SWP,1SWR,1SWT,2F01,2GH7,2IZF,2IZG,2IZH,2IZI,2IZJ,2RTD,2RTE,2RTF,2RTG,2Y3F,3MG5, Not determined. X15 M13 phage display library Streptavidin-coated polystyrene plates were used as an affinity matrix for panning. 892 GAVITH(1) RDIVVA(1) VYSHAS(1) GSYSAG(1) LDIVVA(1) 5 Phage display (subtractive panning) 7 PMID:7496483 Chymotrypsinogen A Not determined. Not determined. Not determined. f3-6mer phage display library (X6) Since BSA was used as a blocking agent in the immobilization procedure, an addition of BSA was made in the seventh round to exclude possible BSA-binding phage clones. 893 VIRYKDVRKYYLGPARAVLT(3) FQKETLMRYWGPGRYFTAY(2) MVNGRSRAVFYYLGPGRLKM(2) SNAVLSGHPQNDYQAFVALR(2) VYAVKQGIRLRYYVGPGRIL(1) EIYGAQGAVGVLFGPGRVWL(1) AAPAGVLHIRHLVGPNRYNF(1) GIFLSEGHSPVNVGPNRLKV(1) VLGAQNTKHLGPGRFWYLL(1) SLVNIPRYFVGPGRRPMLVL(1) SLVPSPLLYIGPGRLRMTDH(1) SVELRSIQLIGIGRNFHWMG(1) MHSFYKGPGRRGYDTLWNQF(1) YILGPGRGRFPGVNCMPYI(1) NTFALVPDWLGSPHGHSHHP(1) PTDTWQAFWLRHPQNHVFLA(1) [YL]-[VLI]-G-P-G-R-x-F 16 Phage display (common panning) 2 PMID:7506691 Anti-HIV-l isotype MN gp120 monoclonal antibody 58.2 Surface protein gp120 of Envelope glycoprotein gp160 Not determined. Not determined. PDL-20 phage display library SA-bio biopanning protocol: mixing phage with biotinylated anti-HIV-l mAB 58.2 in solution overnight at 4°C and then binding to SA coated plate. The bound bacteriophage were washed 12 times with 0.5% Tween20 in PBS, eluted by treatment with 0.1 M HCl, adjusted to pH 2.2 with glycine and neutralized by addition of 1M Tris base. The phage were enumerated by titering transducing units and the phage population amplified by growth in yeast tryptone broth containing 20 p/ml of tetracycline. In subsequent cycles, the biotinylated mAb 58.2 was added to the SA coated plates for 2h at 4°C and the free SA blocked by the addition of bio for 1h at 4°C before the addition of the phage. Washing, elution and amplification of bacteriophage were as above. 894 MDHLGPGRYHWFNRNDRVFK(4) GVRHMGPGRASFDEFQSLHV(3) WVFSPSTGGHRLYTGPGRHF(2) RFMSAYVMKIGPNRHGFRAP(2) RSQGLMVLGPGRGFMIFNAG(2) RRWCRSPNNLFIASPHGPLR(1) AAPAGVLHIRHLVGPNRYNF(1) NLRRTSIRAFYMLGPSRWMP(1) VRKPFQFAASLGPHRAFSHW(1) RAKWNKHLYFEGPSRMFNFT(1) SLVPSPLLYIGPGRLRMTDH(1) ASRYEVFPLLGPHRMMFKFP(1) TEVWSRFWIGPARSLGVDQS(1) FIGGNYKLGRGRANFVNYAT(1) SQLHWRGPGRTRFVSDRFH(1) AASNEFLGAGRLNFDKPKHS(1) RKDGLIGPGRGGFQGMRWVM(1) GRYLLGPGRELVSLVRPAMH(1) RIVLGPARFFFPQSYLLDQG(1) [YL]-[VLI]-G-P-G-R-x-F 19 Phage display (common panning) 2 PMID:7506691 Anti-HIV-l isotype MN gp120 monoclonal antibody 58.2 Surface protein gp120 of Envelope glycoprotein gp160 Not determined. Not determined. PDL-20 phage display library Micropanning protocol: the mAB was diluted to 50 pg/ml in 0.1M NaHCO3, buffer, pH 9.2, and 50pl added to each well of a Nunc Maxisorb microtiter plate. The mAb was bound to the plate for 2h at room temperature, the wells washed with PBS and blocked with 1% powdered milk in PBS for 1h at room temperature. After blocking, the wells were washed four times with PBS, the phage added in 50pl of milk/PBS and bound to the mAb for 1 h at room temperature. The plates were washed 12 times with 0.5% Tween20 in PBS to remove non-specific phage and the phage were eluted by treatment with 50pl of 0.1M HCl adjusted to pH 2.2 with glycine. Eluted phage were immediately neutralized with 10ul of 1M Tris base. 895 RGDFWQ(2) CRGDCA(1) RGDHWT(1) RRGDLI(1) LRGDLH(1) KRGDLG(1) LKRGDI(1) RCDVW(1) NGRAHA(1) RGD 9 Phage display (subtractive panning) 2-3 PMID:7690752 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. f3-6mer phage display library (X6) Phage were elute with GRGDSP peptide. 896 PKRGDL(3) RGDRLF(1) RGDLFI(1) RRGDLR(1) HRRGDL(1) RGDLHF(1) HRGDLH(1) MRRGDL(1) RGDLFL(1) RGD 9 Phage display (common panning) 2-3 PMID:7690752 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. f3-6mer phage display library (X6) Phage were elute with EDTA after the GRGDSP elution. 897 RGDGWL(5) PKRGDL(4) SRRGDL(4) MRGDLR(1) SLIDIP(1) RGD 5 Phage display (common panning) 2-3 PMID:7690752 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. f3-6mer phage display library (X6) Phage were elute with glycine-HCl buffer of pH 2.2 after the GRGDSP and EDTA elution. 898 CRGDCL(6) RGDRPG(2) TIRSVD(2) RGDSWG(1) RRGDLR(1) KRGDLP(1) PKRGDL(1) KRGDLG(1) RGDLHL(1) RGDFWQ(1) GRRGDI(1) RGDLWT(1) RGDGWL(1) RGD 13 Phage display (common panning) 2-3 PMID:7690752 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. f3-6mer phage display library (X6) The panning was performed by coating with a low concentration (10ng/well) of integrin. Phage were elute with GRGDSP peptide. CRGDCL was 10-fold more efficient than any of the linear RGD-containing hexapeptides in inhibiting experiment. 899 CSRGDGFSC(2) CLSRGDTPC(1) CDRRGDGFC(1) CFTRGDAPC(1) CTSRGDMPC(1) CQLRGDGWC(1) CEGRGDWHC(1) CTLRGDNHC(1) CHLRGDGWC(1) CMLRGDSFC(1) CMPRGDGFC(1) CFRGDHVRC(1) CGRGDSVPC(1) CSRGDGFRC(1) CGRGDNLPC(1) CRGDLRFNC(1) RGD 16 Phage display (common panning) 2 PMID:7507494 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. CX7C phage display library In the first and second panning, the coating concentration of the integrin was 5 microgram/well. 900 CIPRGDGWC(5) CTRGDGWFC(3) CFRGDGFKC(2) CRSRGDFPC(1) CVARGDGWC(1) CFRGDFPEC(1) CRRGDGWEC(1) CRGDWPNYC(1) RGD 8 Phage display (common panning) 3 PMID:7507494 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. CX7C phage display library In the first and second panning, the coating concentration of the integrin was 5 microgram/well. In the third round of panning, the coating concentration of the integrin was 1 microgram/well.