851 RFDSLKV(6) GRGDGDV(2) 2 Phage display (subtractive panning) 4 PMID:10913838 Human foetal tracheal epithelial cell line CFT-2 Not determined. Not determined. Not determined. HyC, HyB and HyA phage display library pool Before incubation with the cells, the phage libraries were subtracted to remove phage particles that absorb to polystyrene and to serum proteins. 852 FRTAISGTPQFY(6) SYRTPITGTLIT(2) HSHTHKALAGTP(1) LVAKPHMRTPNL(1) WHWQYTPWWRGS(1) 5 Phage display (common panning) 4 PMID:10880841 Rabbit hyperimmune serum against peptide (MSESHVKISRTIIRGTSPST) AA1–20 of Taenia solium paramyosin (MSESHVKISRTIIRGTSPST) Not determined. Not determined. Ph.D.-12 phage display library (X12) Rabbit hyperimmune serum, diluted 1:50 in PBS to give a final volume 300 ml, was distributed in 6 wells (50 ml:well) of 96-well flat bottom polystyrene microtitration plates. Eight clones presented two sequences with internal RT-I-GT motif and three peptides contained only a part of this motif: RT or GT/S, or T-RGS. Comparison with the amino-terminal 20aa sequence of the TPmy highlighted the Arg10-Thr16 region of apparent homology to the motif shared by two of these peptides and partially by three of them. 853 SLSRMPIIGTSA(4) AWTHTLIRLPD(2) NEMTLIRMNMAA(2) DIRQPIIGTLHP(1) HIARTPIAGTNL(1) HRVPISGTSA(1) LEPTLIRLPQTL(1) MKETHSTLHQP(1) TLIR 8 Phage display (common panning) 3 PMID:10880841 Anti-peptide (MSESHVKISRTIIRGTSPST) polyclonal antibody IgG AA1–20 of Taenia solium paramyosin (MSESHVKISRTIIRGTSPST) Not determined. Not determined. Ph.D.-12 phage display library (X12) Peptide sequences was selected on panning of the 12-mer library in solution. A total of 300 ng of purified IgG fraction were mixed with the library (2e10 phage virions) and incubated 20min at room temperature. Then 50 ml of protein G-Agarose suspension (Gibco BRL) were added and the incubation was continued 15 min at the same temperature. The incubation mixture was centrifuged, supernatant discarded and the pellet washed 10 times with PBS-T. The final pellet was resuspended in 1 ml of glycine-HCl, pH 2.2 and after 10 min incubation at room temperature the eluate was collected after centrifugation The phage were titered, amplified and the selection was repeated two more times, first with 100 ng and then with 30 ng of IgG except for the third round where phage were eluted in two steps, first at pH 4.0 and then at pH 2.2. 854 FPNRTPISGTTW(1) 1 Phage display (common panning) 4 PMID:10880841 Anti-peptide (MSESHVKISRTIIRGTSPST) polyclonal antibody IgG AA1–20 of Taenia solium paramyosin (MSESHVKISRTIIRGTSPST) Not determined. Not determined. Ph.D.-12 phage display library (X12) Peptide sequences was selected on panning of the 12-mer library in solution. A total of 300 ng of purified IgG fraction were mixed with the library (2e10 phage virions) and incubated 20min at room temperature. Then 50 ml of protein G-Agarose suspension (Gibco BRL) were added and the incubation was continued 15 min at the same temperature. The incubation mixture was centrifuged, supernatant discarded and the pellet washed 10 times with PBS-T. The final pellet was resuspended in 1 ml of glycine–HCl, pH 2.2 and after 10 min incubation at room temperature the eluate was collected after centrifugation The phage were titered, amplified and the selection was repeated two more times, first with 100 ng and then with 30 ng of IgG except for the third round where phage were eluted in two steps, first at pH 4.0 and then at pH 2.2. 855 KAAQIYSEP(8)[0.684, 20.0 ± 6.0] DYDGYGWRE(3)[0.360, 9.3 ± 0.2] YAADITNGL(3)[0.648, 11.0 ± 2.3] NKPNWDGYA(1)[0.648, 10.1 ± 0.2] IQWDGYARQ(1)[0.432, ND] WSSGYGTGR(1)[0.234, 8.2 ± 1.0] AYESGYNLP(1)[0.252, 9.7 ± 1.5] CSAGICFSE(1)[0.414, 7.2 ± 1.6] [DS]-G-Y-[AG], A(2)-A-x-I 8 Phage display (common panning) 2 PMID:10759860 Anti-CA monoclonal antibody A-56.36 Crotoxin acid chain, CA Not determined. Not determined. X9 and CX9C phage display library pool ELISA ELISA measurements of the binding to A-56.36 expressed as A490, SD ± 10%. ELISA measurements of the competition with CA, expressed as the inhibition percentage of the binding of A-56.36 to CA-coated plates in the presence of a fixed amount of phages (e12 p.f.u./mL). Average values and SD on two experiments are shown. ND denotes not determined. By screening random phage-displayed libraries with the mAb, phagotopes bearing the (D/S)GY(A/G) or AAXI consensus motifs were selected. They all bound A-56.36 in ELISA and competed with CA for mAb binding, although with different reactivities. 856 DFFEII(1) DFLMLV(1) QFVFCW(1) DFLVQL(1) DFLAIV(1) 5 Phage display (common panning) 2 PMID:1696028 Anti-Myohemerythrin monoclonal antibody A2 Myohemerythrin, MHr Not determined. Not determined. f3-6mer phage display library (X6) In the first round, 1e12 phage particles were incubated overnight with 1000nM bio-MAb in 10 microliter reaction mixtures; the phages were diluted and reacted 10 to 20 minutes on streptavidin-coated dishes; the dishes were extensively washed; and the bound phages were eluted in acid buffer. Phages eluted in the first round were amplified on agar medium and subjected to second round of paning with the same bio-MAb at concentrations of 10 nM. 857 DFLAIV(4) DFLEYI(2) DFLEIL(1) DFLEIV(1) 4 Phage display (common panning) 2 PMID:1696028 Anti-Myohemerythrin monoclonal antibody A2 Myohemerythrin, MHr Not determined. Not determined. f3-6mer phage display library (X6) In the first round, 1e12 phage particles were incubated overnight with 1000nM bio-MAb in 10 microliter reaction mixtures; the phages were diluted and reacted 10 to 20 minutes on streptavidin-coated dishes; the dishes were extensively washed; and the bound phages were eluted in acid buffer. Phages eluted in the first round were amplified on agar medium and subjected to second round of paning with the same bio-MAb at concentrations of 0.1 nM. 858 DFLAWL(14) DFLEML(2) 2 Phage display (common panning) 3 PMID:1696028 Anti-Myohemerythrin monoclonal antibody A2 Myohemerythrin, MHr Not determined. Not determined. f3-6mer phage display library (X6) In the first round, 1e12 phage particles were incubated overnight with 1000nM bio-MAb in 10 microliter reaction mixtures; the phages were diluted and reacted 10 to 20 minutes on streptavidin-coated dishes; the dishes were extensively washed; and the bound phages were eluted in acid buffer. Phages eluted in the first round were amplified on agar medium and subjected to second round of paning with the same bio-MAb at concentrations of 0.1 nM. Phages from the second round were amplified and subjected to third round with 0.1 nM bio-MAb. 859 DFLEWL(4) DFMEWL(1) DFLEQL(1) DFLHFI(1) AWERRG(1) 5 Phage display (common panning) 2 PMID:1696028 Anti-Myohemerythrin monoclonal antibody M33 Myohemerythrin, MHr Not determined. Not determined. f3-6mer phage display library (X6) In the first round, 1e12 phage particles were incubated overnight with 1000nM bio-MAb in 10 microliter reaction mixtures; the phages were diluted and reacted 10 to 20 minutes on streptavidin-coated dishes; the dishes were extensively washed; and the bound phages were eluted in acid buffer. Phages eluted in the first round were amplified on agar medium and subjected to second round of paning with the same bio-MAb at concentrations of 10 nM. 860 CRFVWC(2) DFLERI(1) CELEKC(1) CRLEKC(1) DFMEWL(1) DFLVQL(1) 6 Phage display (common panning) 2 PMID:1696028 Anti-Myohemerythrin monoclonal antibody M33 Myohemerythrin, MHr Not determined. Not determined. f3-6mer phage display library (X6) In the first round, 1e12 phage particles were incubated overnight with 1000nM bio-MAb in 10 microliter reaction mixtures; the phages were diluted and reacted 10 to 20 minutes on streptavidin-coated dishes; the dishes were extensively washed; and the bound phages were eluted in acid buffer. Phages eluted in the first round were amplified on agar medium and subjected to second round of paning with the same bio-MAb at concentrations of 0.1 nM. 861 DFLEWL(16) DFLEML(1) 2 Phage display (common panning) 3 PMID:1696028 Anti-Myohemerythrin monoclonal antibody M33 Myohemerythrin, MHr Not determined. Not determined. f3-6mer phage display library (X6) In the first round, 1e12 phage particles were incubated overnight with 1000nM bio-MAb in 10 microliter reaction mixtures; the phages were diluted and reacted 10 to 20 minutes on streptavidin-coated dishes; the dishes were extensively washed; and the bound phages were eluted in acid buffer. Phages eluted in the first round were amplified on agar medium and subjected to second round of paning with the same bio-MAb at concentrations of 0.1 nM. Phages from the second round were amplified and subjected to third round with 0.1 nM bio-MAb. 862 RWWWPTR RWWWLPR RWWWFPR KWWMASR RWWWDPF RWWWSFS VKWWWSA IWKWWWK 8 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab003 Not determined. Not determined. Not determined. X7 phage display library The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 863 FCSFCVL FCSDCIL FCGDCVL 3 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab004 Not determined. Not determined. Not determined. X7 phage display library The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 864 CRWWSGQPC CRWWWQDTC CRWWMGVSC CRWWNGSWC CRWWMSYTC CRWWRGQGC CRWWFGQFC CRWWRGEVC CRWWLGSAC CRWWAGSTC CRWWWVETC CKWWGGRGC 12 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab003 Not determined. Not determined. Not determined. CX7C phage display library The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 865 WNWPLPPVRQFS WPWPLPPEPPLG SWYWPLPPWRLG SWPWYHPHIKSH 4 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab001 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 866 TPEKWHRLLTMS ATPWSQWLDAPR 2 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab003 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 867 EMSVDWWSPISS ASSVDWWPVRPP FMWPDQNPRHSM 3 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab004 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 868 QLLFPSSPRAPAPWTFTF 1 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab006 Not determined. Not determined. Not determined. X18 phage display library The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 869 YYCYFTEAPYSYWGNLVC SQSPPRYWAWCAGYWCEL 2 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab007 Not determined. Not determined. Not determined. X18 phage display library The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 870 SYWPCHPGTRHCSNRV 1 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab003 Not determined. Not determined. Not determined. Beta-sheet (BS) phage display library (X4CX6CX4) The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 871 PVLVCGPKWSNCSPAN SPSLCWPWLEQCTEGI GDQPCPIFDRECHKPT GIGPCELIDSECEASE 4 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab004 Not determined. Not determined. Not determined. Beta-sheet (BS) phage display library (X4CX6CX4) The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 872 YHRWCVMDRRACFEAP NLSECAMPTRKCSRTA 2 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab005 Not determined. Not determined. Not determined. Beta-sheet (BS) phage display library (X4CX6CX4) The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 873 LEYACNGPSQLCSYVR LPWACPFTGWFCDLIT TEKTCAGPGDLCLLVR AQRKCAGNWAICRLVY 4 Phage display (subtractive panning) 3/4 PMID:21117166 Chronic Lymphocytic Leukemia Fragment Antigen-Binding (Fab) Fragments Fab006 Not determined. Not determined. Not determined. Beta-sheet (BS) phage display library (X4CX6CX4) The library was screened on BCR Fab fragments after a 2-fold negative selection on polyclonal human immunoglobulins (Octapharma, Lachen, Switzerland). 874 SWLSRRPLPPLPP(2) VPLARRPLPPLPP(2) SSLRFRPLPPLPP(2) WSLFHRPLPPLPP(1) CLLCSRPLPPLPP(1) IHLRTRPLPPLPP(1) PHPARRPLPPLPP(1) VSLAQRPLPPLPP(1) ISLARRPLPPLPP(1) SWLANRPLPPLPP(1) LSLQMRPLPPLPP(1) SSLSRRPLPPLPP(1) LSLAQRPLPPLPP(1) LSLFRRPLPPLPP(1) SWLSHRPLPPLPP(1) SSLCGRPLPPLPP(1) 16 Phage display (common panning) 4 PMID:7479908 Src SH3 domain of Proto-oncogene tyrosine-protein kinase Src Not determined. Not determined. Not determined. N+5 class I phage display library GST-Src SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target. 875 SAPSRRPLPPLPPP(1) LRWSARPLPPLPPP(1) KALASRPLPPLPPP(1) TTLSARPLPPLPPP(1) SYLSRRPLPPLPPP(1) LSPMSRPLPPLPPP(1) LPMARRPLPPLPPP(1) ASFASRPLPPLPPP(1) VGLSNRPLPPLPPP(1) IPIGHRPLPPLPPP(1) IALSTRPLPPLPPP(1) FFLASRPLPPLPPP(1) LDRSRRPLPPLPPP(1) RWITSRPLPPLPPP(1) GLGARRPLPPLPPP(1) DDLSGRPLPPLPPP(1) NPLLRRPLPPLPPP(1) 17 Phage display (common panning) 4 PMID:7479908 Fyn SH3 domain of tyrosine-protein kinase Fyn Not determined. Not determined. Not determined. N+5 class I phage display library GST-Fyn SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target.