801 CLDTAKYFWC(2) CSCAGKMWCRC(2) CADGTSKVRLC(1) CLVSERLC(1) CLSKDLASSPMC(1) CDWGDLESSVLC(1) CLASYRFGDRYC(1) CWMGWKCSDTGC(1) CDPISMLGKWYC(1) CLVEGWCNPWVC(1) 10 Phage display (common panning) 3 PMID:10029604 Human neutrophil cells Not determined. Not determined. Not determined. CL10 phage display library (CX10C) Freshly isolated PMN were incubated with 20 μL aliquots of phage library in 1 mL HBSS containing 0.1% bovine serum albumin (phage buffer) for 2 hours at 4°C in a 1.5 mL microcentrifuge tube. PMN were then washed five times with phage buffer and bound phage were eluted for 5 minutes in 2 mL of 0.1 mol/L glycine (pH 2.2). 802 CNLVTTTKYNPC(9) CSCLVTEKFSSC(1) CEIQTTKMGMAC(1) 3 Phage display (common panning) 3 PMID:10029604 Human neutrophil cells Not determined. Not determined. Not determined. CL10 phage display library (CX10C) Freshly isolated PMN were incubated with 20 μL aliquots of phage library in 1 mL HBSS containing 0.1% bovine serum albumin (phage buffer) for 2 hours at 4°C in a 1.5 mL microcentrifuge tube. PMN were then washed five times with phage buffer and bound phage were eluted for 5 minutes in 2 mL of 0.1 mol/L glycine (pH 2.2) followed by addition of phage buffer containing 0.5% Tween 20 to the remaining cell pellet. 803 CWWDGMCAALKC(3) CWPSWMCDRLQC(2) CLVEGWCNPWVC(2) CRNCGWDRPNIC(2) CPFGPDLQGKWC(1) CRSNMWLGNWLC(1) CSKDSWLGRIYC(1) CLVKDWCGTMRC(1) CLGDWWQRWELC(1) CTEWGGMWNILC(1) CSMWGGMWEMFC(1) CGSWEMWGKEEC(1) CGRWPTWGDPRC(1) CGDPGTWELLWC(1) CRGMWALLNVAC(1) 15 Phage display (common panning) 3 PMID:10029604 Human neutrophil cells Not determined. Not determined. Not determined. CL10 phage display library (CX10C) Freshly isolated PMN were incubated with 20 μL aliquots of phage library in 1 mL HBSS containing 0.1% bovine serum albumin (phage buffer) for 1 hour at 20°C in a 1.5 mL microcentrifuge tube. PMN were then washed five times with phage buffer and bound phage were eluted for 5 minutes in 2 mL of 0.1 mol/L glycine (pH 2.2). 804 CKDGLFLGSWLC(1)[1.46 ± 0.09] CTRSMFLGEWLC(1)[NT] CMAGVFLGALGC(1)[NT] CDVGALLGPWLC(1)[NT] CNLGNFLGSWLC(1)[NT] CNSEGPLGRWYC(1)[NT] CRKGTLLGEYFC(1)[NT] CADGLLLGKWYC(1)[NT] CNSQGILGAWYC(1)[NT] CDPRQWLGQYLC(1)[NT] CARNGFLGNWLC(1)[NT] CGKDSFLGMWLC(1)[NT] CDKKSWLGSWLC(1)[NT] CDRKFFLGKNWC(1)[NT] CRNCGWDRPMIC(1)[NT] CSTCGWNNEMWC(1)[NT] CLVKDWCGTMRC(1)[NT] 17 Phage display (common panning) 3 PMID:10029604 Human neutrophil cells Not determined. Not determined. Not determined. CL10 phage display library (CX10C) ELISA Absorbance at 405 nm was measured. Data were reproduced from the graph and expressed as representative of one of two experiments showing the mean ± SD of quadruplicate determinations. NT represents not tested. Freshly isolated PMN were incubated with 20 μL aliquots of phage library in 1 mL HBSS containing 0.1% bovine serum albumin (phage buffer) for for 1 hour at 20°C in a 1.5 mL microcentrifuge tube. PMN were then washed five times with phage buffer and bound phage were eluted for 5 minutes in 2 mL of 0.1 mol/L glycine (pH 2.2) followed by addition of phage buffer containing 0.5% Tween 20 to the remaining cell pellet. 805 CQQFLSVRALC CGGTPPSLPTC CGGTRRPIASC CFPYCYPSESA CSEYREPLLC 5 Phage display (common panning) 4 PMID:11053238 Anti-Bet v 1 polyclonal antibody IgE Major pollen allergen Bet v 1-A Not determined. Not determined. pVIII-9aa.Cys phage display library (CX9C) 806 AGTHTPGAT TSKASELAV 2 Phage display (common panning) 4 PMID:11053238 Anti-Bet v 1 polyclonal antibody IgE Major pollen allergen Bet v 1-A Not determined. Not determined. pVIII-9aa phage display library (X9) 807 AAHMQRFPLLHQ(4) FSKPSAALTHDT(3) MSPMHKQSRATY(3) SFLTPWFTHFPR(2) LATTKPSHLTRL(1) SIHHWPMLFRLP(1) LSPFPKYPPKIP(1) VSHLPTLKMPSA(1) 8 Phage display (common panning) 4 PMID:10936026 Anti-gpIIIa monoclonal antibody Y2/51 Integrin beta-3 Not determined. Not determined. Ph.D.-12 phage display library (X12) 808 HTYSWPTLHPNG(2) MTFSTKGSTTSG(1) STMHHHLAPFIA(1) YVHSTQPLTPQN(1) TPVMPPRWAVAL(1) SSHVQSWRLPKA(1) QGLPFHAALRLL(1) IRPWIWTPDIRS(1) YNTTTPVPQDLS(1) IPLHWHADQTRW(1) LPSSIRLHSHFP(1) FPFQHMSLKHID(1) FSTSPSYSPVWR(1) SWHVWPYHQDRS(1) TPVSNDDLAQKR(1) 15 Phage display (common panning) 4 PMID:10936026 Anti-gpIIb/IIIa monoclonal antibody 5B12 Integrin alpha-IIb/beta-3 Not determined. Not determined. Ph.D.-12 phage display library (X12) 809 LAHPTSGHTLPW(3)[2.22, 1.53] SGLAKFCLAPLP(2)[NT, NT] DVLMHGFDPALI(1)[0.70, 1.53] WRILASGHTLPW(1)[NT, 1.17] QIWHHYPNSQLL(1)[NT, NT] LEMFPDKNQRLT(1)[NT, NT] AKTMVIFLRRRL(1)[NT, NT] YLQSNTEPKQDS(1)[NT, NT] MFPMGMSQRPFS(1)[NT, NT] NRTPEILHSIMH(1)[NT, NT] SVRSPHLFPVMR(1)[NT, NT] TTHHYGYKSGY(1)[NT, NT] 12 Phage display (common panning) 4 PMID:10936026 Anti-human platelet polyclonal antibody Human platelet Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Two types of ELISA were performed. In the first set of experiments all phage clones were evaluated using plates coated directly with phage. In the second set of experiments plates were previously coated with anti-M13 monoclonal antibody, then phage (only phage clones selected with rabbit antihuman platelet antibody) were added. In this case anti-M13 monoclonal antibody reacted with pVIII protein of phage particle, and peptides expressed as fusion with pIII would be accessible for serum IgG. The absorbance was measured at 405 nm, and each OD value represents the mean of duplicate determinations. Platelet lysate was used as positive control. Non-related phage GM6 was used as negative control. Data shown were reproduced from the graph. NT denotes not tested. 810 LASLCNPKPSDAPVT(9) LVRAYAGLARIILKW(4) YASPSLFGFPFALEA(2) ICACLCCACSYNSXV(2) KAHDLSTASRPHVVT(1) PAGEIKVGKLRQLQR(1) SQYGIRGSFLLYVLP(1) 7 Phage display (common panning) 3 PMID:9698107 Anti-chlamydia pneumoniae monoclonal antibody RR-402 Chlamydia pneumoniae Not determined. Not determined. f88-15mer phage display library (X15) LASLCNPKPSDAPVT showed higher ELISA reactivity with C. pneumoniae MIF-positive sera compared to patients with other chlamydial infections, non-chlamydial respiratory infections and normal healthy sera (MIF-negative). 811 GLISDSGFLIPSKRR(2) FVKDRPLELRTFGTL(2) GTVTLGRFSRLSLFG(2) VRVLWGGFSRLSLFX(1) PTTPKSILHFLFPLF(1) LASSVKSAEVGCAGY(1) SVTSATPRGERHVSP(1) QFCGWGEFEVRVLWP(1) NSKGKMNTCHKVGNS(1) 9 Phage display (common panning) 3 PMID:9698107 Polyclonal sera from patients with C. pneumoniae infection Chlamydia pneumoniae Not determined. Not determined. f88-15mer phage display library (X15) LASLCNPKPSDAPVT showed higher ELISA reactivity with C. pneumoniae MIF-positive sera compared to patients with other chlamydial infections, non-chlamydial respiratory infections and normal healthy sera (MIF-negative). 812 VGFALFNARY KYNALYGVRT KGNARYTLLS GWNAQYEWRG KWNGAYRSER VARLNSSYRN N-A-x-Y 6 Phage display (common panning) 3 PMID:9675087 Anti-SHP-1 monoclonal antibody 6D41 Tyrosine-protein phosphatase non-receptor type 6 Not determined. Not determined. PDL-10R phage display library 813 LLYNALYGRE GLYGWNSGYS MKYNAQYRWD PHYNAHYQRG SRYTWNSRYT RMYNALYEAS AWYKLNAGYS N-A-x-Y 7 Phage display (common panning) 3 PMID:9675087 Anti-SHP-1 monoclonal antibody 6D41 Tyrosine-protein phosphatase non-receptor type 6 Not determined. Not determined. PDL-10Y phage display library 814 SKRPLWPVFA PWWPARNRPP MPNWPVLRGP GNPPAWPAPG PAWPDLRGWV DKVTPFWPAL KMPWWPFLEV KLKGPWWPKA PRWPWLEGVG P-x-W-P 9 Phage display (common panning) 3 PMID:9675087 Anti-SHP-1 monoclonal antibody 3E7 Tyrosine-protein phosphatase non-receptor type 6 Not determined. Not determined. PDL-10R phage display library 815 RPYWPIKNLA SPYWPYRMWT KPYWPGGGEA HPYWPDTSRS PYWP 4 Phage display (common panning) 3 PMID:9675087 Anti-SHP-1 monoclonal antibody 3E7 Tyrosine-protein phosphatase non-receptor type 6 Not determined. Not determined. PDL-10Y phage display library 816 GFVWYGLNIK ILDWYGVRID LDWYGILLET RWYDLILLQD WY 4 Phage display (common panning) 3 PMID:9675087 Anti-SHP-1 monoclonal antibody 4E6 Tyrosine-protein phosphatase non-receptor type 6 Not determined. Not determined. PDL-10R phage display library 817 MWYGVEVPWS ELYWYGLPIR YWYGLEIPSA WY 3 Phage display (common panning) 3 PMID:9675087 Anti-SHP-1 monoclonal antibody 4E6 Tyrosine-protein phosphatase non-receptor type 6 Not determined. Not determined. PDL-10Y phage display library 818 KHVQYWTQMFYS(5) DFLQWKLARQKP(1) 2 Phage display (common panning) 4 PMID:9862799 Texas red fluorophore dye Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Each biopanning round consisted of four sequential steps: phage binding with a fluorophore dye carrier, washing unbound phage from the beads, nonspecific elution of bound phage and amplification of bound phage. 819 IPHPPMYWTRVF(6) 6 Phage display (common panning) 4 PMID:9862799 Rhodamine red fluorophore dye Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Each biopanning round consisted of four sequential steps: phage binding with a fluorophore dye carrier, washing unbound phage from the beads, nonspecific elution of bound phage and amplification of bound phage. 820 HGWDYYWDWTAW(4) ASDYWDWEWYYS(2) YPNDFEWWEYYF(2) HTSHISWPPWYF(1) LEPRWGFGWWLK(1) QYYGWYYDH(1) YMYDEYQYWNFW(1) 7 Phage display (common panning) 4 PMID:9862799 Oregon green 514 fluorophore dye Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Each biopanning round consisted of four sequential steps: phage binding with a fluorophore dye carrier, washing unbound phage from the beads, nonspecific elution of bound phage and amplification of bound phage. 821 YPNDFEWWEYYF(7) ASDYWDWEWYYS(2) WYDDWNDWHAWP(2) WHMSPSWGWGYW(1) HMSWWEFYLVPP(1) YWDYSWHYYAPT(1) 6 Phage display (common panning) 4 PMID:9862799 Fluorescein fluorophore dye Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Each biopanning round consisted of four sequential steps: phage binding with a fluorophore dye carrier, washing unbound phage from the beads, nonspecific elution of bound phage and amplification of bound phage. 822 GYARTFWLPGLWSVP(8)[+] WVSHFSVFAFPMSSW(4)[++] AGLHLFWSYSSYVTP(4)[++] SGPFIWNFWLGARAV(3)[+++] RLLVFRYWNLFPNVS(1)[-] GVRVPFWEGLEFFTL(1)[+/-] GSLIVPEWHLAPPGL(1)[-] TNLPPIFYDLHCFAF(1)[+] SGWSAFYNVAFFSTR(1)[++] RNLPPIFNDVYWIAF(1)[-] 10 Phage display (common panning) 4 PMID:10449040 U1 small nuclear RNA, U1 snRNA Not determined. Not determined. Not determined. f3-15mer phage display library (X15) Dot blot Fourth-round clones were initially screened using dot blots. Intensity of each dot was determined subjectively and is indicated as being either strong (+++), moderate (++), weak (+), very weak (+/-), or absent (-) relative to the negative control. 823 AGLHLFWSYSSYVTP(8) FRSWMHFVTYVSTGG(8) EAWARWVFAWLWGHG(6) GYARTFLWPGLWSVP(5) WAYYHAGHSSFAVWT(3) GPQSATSCCYVSTGG(3) YRVSLSVLHNSLLPS(2) VVPRFWFWLHGPGPF(1) VVRAFLGISLGLWSV(1) AHLYFDSGWASRHWT(1) 10 Phage display (common panning) 5 PMID:10449040 U1 small nuclear RNA, U1 snRNA Not determined. Not determined. Not determined. f3-15mer phage display library (X15) 824 SISPWGFSGLLRWSY(28)[+++] TYALTSSILVYLTSS(1)[+] EVPRLSLLAVSLVAN(1)[+] TVSAGIICSFLSVSC(1)[+] VFQFRASVGGSHTVI(1)[-] RLVCWRLGCVSPMGS(1)[+/-] KWPVAVTLAAGIRIP(1)[+/-] SVLSHVRTVVLFSRS(1)[-] LRYTLETRWAVLSVY(1)[++] LILFSYDFHGDYYFY(1)[-] 10 Phage display (common panning) 4 PMID:10449040 Anticodon stem and loop of yeast tRNA(Phe) (tRNA(ACPhe)) Not determined. Not determined. Not determined. f3-15mer phage display library (X15) Dot blot Fourth-round clones were initially screened using dot blots. Intensity of each dot was determined subjectively and is indicated as being either strong (+++), moderate (++), weak (+), very weak (+/-), or absent (-) relative to the negative control. 825 SISPWGFSGLLRWSY(42) AAGGFFRWIFSPVYV(5) VSVYYGSFCLHSAYG(1) NNHPWYYGRSHHVSG(1) VFQFRASVGGSHTVI(1) TFRLLALWRGGLYPG(1) 6 Phage display (common panning) 5 PMID:10449040 Anticodon stem and loop of yeast tRNA(Phe) (tRNA(ACPhe)) Not determined. Not determined. Not determined. f3-15mer phage display library (X15)