776 CEYSDSGIEVC CALSENKLRVC CHSSENRSSC CPTSENRAHSC CLPSPLLENRC CSLYENRRVC CIKNPSSENAC CFTPEGDHRPC CQFFPEGDYLC CSDIDLLSQVC CRQDLSTLDC CRASVDRDLAC 12 Phage display (common panning) 3 PMID:9671121 Anti-BLG polyclonal antibody Beta-lactoglobulin, Beta-LG Not determined. Not determined. pVIII-9aa.Cys phage display library (CX9C) 777 YSPFSW[37, 110] YVSWSPD[35, 100] FSWSDT[19, 90] YSWSDM[10, 60] WSPFPS[26, 60] DSPFSF[22, 80] FSPFDW[21, 90] TSPFPW[31, 90] YSFFPW[16, 70] YSDFPW[26, 60] DSWFPW[14, 20] SSFYSS[22, 90] DSWSTS[NT] FSPFSF[NT] SSPFDW[NT] 15 Phage display (common panning) 8 PMID:10547288 Protein Nef Tyrosine-protein kinase HCK Not determined. Not determined. RRT-SH3 phage display library Binding assay The relative potency of the corresponding SH3 domains to compete with labeled Hck-SH3 Nef and RRT.A1 Nef in binding to Nef, respectively, expressed as probe:competitor ratio at 50% inhibition. NT denotes not tested. To examine if it would be possible to present functional SH3 domains on the surface of bacteriophages authors constructed a phagemid containing Hck-SH3, and by using a M13KO7 helper virus produced recombinant phages expressing it fused to the pIII coat protein. 778 QGFLDQ[60] NAFLPS[40] EAWSPL[80] ESYSEW[10] 4 Phage display (common panning) 8 PMID:10547288 Protein NefR90 Tyrosine-protein kinase HCK Not determined. Not determined. RRT-SH3 phage display library Binding assay The relative potency of the corresponding SH3 domains to compete with labeled RRT.A1 NefR90 in binding to NefR90, expressed as probe:competitor ratio at 50% inhibition. To examine if it would be possible to present functional SH3 domains on the surface of bacteriophages authors constructed a phagemid containing Hck-SH3, and by using a M13KO7 helper virus produced recombinant phages expressing it fused to the pIII coat protein. 779 SPTRAVNELFGM(11)[0.21] SLPRVYYEIFGA(6)[0.40] HTNLPQALWGMQ(5)[0.10] KLAQTFPQVLLA(5)[NT] HNNQRLMLYGMH(3)[0.05] HWPFLQWHMYYP(3)[NT] YTAAPRYMAELF(2)[NT] YVGPLTQTLYGM(2)[0.08] GHPRTAELLGML(1)[0.19] LRPFHQILYGMS(1)[0.14] TLNWPPTLWGMH(1)[0.05] YFWPMTKFQLWR(1)[0.05] TASTLHKTLFGM(1)[NT] FYGLHASLFGMR(1)[NT] HWSHAFMSVWGM(1)[NT] QMLNRLSELNGM(1)[NT] SLVELPSPPMLA(1)[NT] TFLANPSSNLVM(1)[NT] SFAASEPDRART(1)[NT] TSFTGWWNLMAR(1)[NT] GPEQLLFLRPFP(1)[NT] APMHKALWVQPG(1)[NT] GQLQFSNRSLSV(1)[NT] MYNDRTRSFIAS(1)[NT] FNSSHSLLRRPP(1)[NT] QIRTLPIYSLQS(1)[NT] YHWWQFQMKHSA(1)[NT] YRWWPFQVKPSA(1)[NT] WWHPKIPSPSAR(1)[NT] WWQFPWPPYTSV(1)[NT] NSVIGCRTQSCD(1)[NT] NQLIASLSPRVD(1)[NT] RFPWFFMESSHS(1)[NT] 33 Phage display (common panning) 4 PMID:11275260 Anti-NTX monoclonal antibody BNTX18 Potassium channel toxin alpha-KTx 2.1 Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Absorbance at 405 nm was measured. Each value represents mean of triplicates. Data shown were reproduced from the graph. NT denotes not tested. We used the biopanning procedure in two conditions: with the mAb immobilized on a plastic surface (three rounds) and in solution (the final, fourth, round). In total authors determined the sequences of 33 different peptides displayed by 59 clones isolated from the eluates after the third and the fourth rounds of panning of the 12-mer peptide library. 780 MVLWGMH(7) QWIFGMS(1) EQWLGMS(1) EALYGMS(1) QWLYGMS(1) AIAPFFA(1) PHLPMQS(1) SIASLSR(1) SDSSTLN(1) HTTQHRL(1) 10 Phage display (common panning) 3 PMID:11275260 Anti-NTX monoclonal antibody BNTX18 Potassium channel toxin alpha-KTx 2.1 Not determined. Not determined. Ph.D.-7 phage display library (X7) In total authors determined the sequences of 16 clones containing ten different peptides derived from the 7mer library. 781 CIVPKPASEQC[0.91] CLVPRRPPPQC[0.66] CVFLTAQTVPC[0.78] CVAYETAIASC[0.67] CFIPPINPATC[0.64] CRPPLRTKKSC[0.59] CGPAAARLDMC[0.54] CFVPAVQPATC[0.84] CIISPRSVSPC[1.05] CLVPAPIIALC[0.61] 10 Phage display (common panning) 3 PMID:10381087 Cucumber mosaic virus, CMV Not determined. Not determined. Not determined. pVIII-9aa.Cys phage display library (CX9C) ELISA Absorbance at 405 nm was measured. Background signals of 0.11, obtained with M13KO7 helper phage, were subtracted from signals produced with peptide displaying phage. Data shown were reproduced from the graph. CMV was also isolated in house from 50g of infected Nicotiana cle6elandii leaf tissue. Sequencing of the genes encoding 10 of these peptides revealed an absence of any conserved motifs, although nine of them contained a high proportion of proline residues. 782 CVFTSDYAFC(2)[7.8] CVIYDGNHWC(2)[NT] CIFEPDYSYC(2)[NT] CVFDDLYSFC(1)[NT] 4 Phage display (common panning) 3 PMID:11012675 Prostate-specific antigen Not determined. Not determined. Not determined. CX8C phage display library Surface plasmon resonance (SPR) Equilibrium dissociation constant (KD, μM) was measured. The values shown are the average KD of three different concentrations of analyte (GST-peptides). PSA was immobilized on microtiter wells coated with the monoclonal IgG 5E4, which binds both free and complexed PSA. 783 CTFSVDYKYLMC(15)[NT] CVFAHNYDYLVC(2)[3.5] CRFDKEYRTLVC(1)[NT] 3 Phage display (common panning) 3 PMID:11012675 Prostate-specific antigen Not determined. Not determined. Not determined. CX10C phage display library Surface plasmon resonance (SPR) Equilibrium dissociation constant (KD, μM) was measured. The values shown are the average KD of three different concentrations of analyte (GST-peptides). PSA was immobilized on microtiter wells coated with the monoclonal IgG 5E4, which binds both free and complexed PSA. 784 CLSTCAQSCRISC(7) CLLYCHDACWWVC(2) 2 Phage display (common panning) 3 PMID:11012675 Prostate-specific antigen Not determined. Not determined. Not determined. CX3CX3CX3C phage display library PSA was immobilized on microtiter wells coated with the monoclonal IgG 5E4, which binds both free and complexed PSA. 785 CVEYCWEGSCYVC(7)[NT] CVAYCIEHHCWTC(3)[2.9] CVSYCLFEFCYVC(2)[NT] CVAYCEEWECYVC(1)[NT] CVSYCDGLQCWMC(1)[NT] 5 Phage display (common panning) 3 PMID:11012675 Prostate-specific antigen Not determined. Not determined. Not determined. CX3CX4CX2C phage display library Surface plasmon resonance (SPR) Equilibrium dissociation constant (KD, μM) was measured. The values shown are the average KD of three different concentrations of analyte (GST-peptides). PSA was immobilized on microtiter wells coated with the monoclonal IgG 5E4, which binds both free and complexed PSA. 786 DPGVEVWLGN(5) GGTTVWLGEG(3) GTEVWLWGGG(3) RGITVVLGKS(2) WGTEVWLGMG(1) RGVTVWLGGT(1) RGVTVWLGQF(1) LVGTDIWLWT(1) G-x(2)-V-W-L-G 8 Phage display (common panning) 3 PMID:9894603 PDZ2 domain of hPTPE1 Not determined. Not determined. Not determined. PDL-10R phage display library PDZ2 expressed as a fusion protein (GST-PDZ2) was immobilized in wells of microplates, and affinity panning was done. 787 KAYGTVVWLG(2) NSYGTVVWLG(1) VVYGTVVWLG(1) DSYGIEVWLG(1) WEYGIEVWLG(1) G-x(2)-V-W-L-G 5 Phage display (common panning) 3 PMID:9894603 PDZ2 domain of hPTPE1 Not determined. Not determined. Not determined. PDL-10Y phage display library PDZ2 expressed as a fusion protein (GST-PDZ2) was immobilized in wells of microplates, and affinity panning was done. 788 QKRAKGLSA(8)[0.441 ± 0.060] YRKKSSAEL(2)[0.496/0.363] MRKANSPPT(2)[0.491/0.401] ARKVKGAAG(2)[0.404/0.384] RGLKSKIAM(1)[0.466] FRKTSISGA(1)[0.421] LSRKKTLTT(1)[0.421] RSLKTKLPS(1)[0.402] LRKKGYDPG(1)[0.400] SRKKTFTGA(1)[0.400] TKLSNTPSP(1)[0.396] GRKSAVSPA(1)[0.358] HRKSTVAGS(1)[0.345] FRKTSKGGS(1)[0.341] HKKSLSSPS(1)[0.338] RRKPGTEQL(1)[0.332] YRKTAKEGT(1)[0.323] YKKGARPIQ(1)[0.323] RK 18 Phage display (subtractive panning) 3/5 PMID:11034389 Anti-GAD65 monoclonal antibody MICA3 Glutamate decarboxylase 2 Not determined. Not determined. pVIII-9aa.Cys phage display library (CX9C) ELISA The reactivity of phagotopes expressing that peptide with MICA3, as determined by capture ELISA, is given as an OD value measured at 405 nm. After each round of positive selection, there was a negative selection step in which phage not specifically reactive with the mAbs were removed using magnetic beads coated with anti-human Ig in the absence of primary Ab. Twenty-eight of 35 phagotopes from the fifth round of positive selection and 0 of 32 phage from the third round of positive selection were reactive by capture ELISA. 789 MRKSTGTAS(2)[0.461/0.473] QKKMVALSG(1)[0.733] SRKVALQGG(1)[0.639] RRKLRTNAG(1)[0.631] RKRTRHLEG(1)[0.562] TQDLGYSRV(1)[0.561] RKKKPPGLA(1)[0.554] TRKALTQNT(1)[0.541] NRKLKSNMM(1)[0.523] KRRASAGGP(1)[0.491] RKVVQTSLE(1)[0.476] KGKKALQSA(1)[0.467] VRKLTVTSA(1)[0.446] KKTKGLVTT(1)[0.435] KSVKPRQAT(1)[0.434] FRKADKFPM(1)[0.434] RKTALGTRQ(1)[0.426] SRKSGSLTK(1)[0.425] KKKKYAEAV(1)[0.400] ARLSVVRNG(1)[0.370] SRKATASLP(1)[0.367] TRGLKGAPQ(1)[0.320] RKLAHPGTS(1)[0.308] GGTTTPRTT(1)[0.294] NRRLLPMPE(1)[0.290] V-A-L-x-G 25 Phage display (subtractive panning) 3/5 PMID:11034389 Anti-GAD65 monoclonal antibody MICA4 Glutamate decarboxylase 2 Not determined. Not determined. pVIII-9aa.Cys phage display library (CX9C) ELISA The reactivity of phagotopes expressing that peptide with MICA4, as determined by capture ELISA, is given as an OD value measured at 405 nm. After each round of positive selection, there was a negative selection step in which phage not specifically reactive with the mAbs were removed using magnetic beads coated with anti-human Ig in the absence of primary Ab. Twelve of 14 phage from the fifth round of positive selection and 14 of 21 phage from the third round of positive selection reacted by capture ELISA. 790 WDPAMNVSLQNS(4) YDPAQGRIAGIR(1) TDPAHTPFPHTR(1) WDPAQQVRHQDN(1) SDPAQAVGRMRP(1) GGSGTSRTPILG(1) IHLPIASASQMT(1) 7 Phage display (common panning) 1 PMID:11602054 Anti-E1 protein polyclonal antibody IgG HCV E1 protein Not determined. Not determined. Ph.D.-12 phage display library (X12) 791 NLIGHHFPHFRALSS TWPVVHGACRAHGHC GGFSLHPWWRFYHDR THSHQWRHHQFPAPT QHGHFTDGYHLPSRL GFTDVHLHLPGNSHR LTLSHPHWVLNHFVS 7 Phage display (common panning) 4 PMID:12174288 HrNMT Not determined. Not determined. Not determined. f3-15mer phage display library (X15) 792 TWPVVHGACRAHGHC NLIGHHFPHFRALSS GLDLLGAVRKPVVRR GALVLSRVDRWFLVA TASLVFFRDGTLSNR LVRHGRYLVTAWHSY AFVALGSLSYGLHGP FAFGFFGGRVWIPRG EFLHLSPTGGVWLAS AGPLRGFYMCSWCTP 10 Phage display (common panning) 4 PMID:12174288 Glycylpeptide N-tetradecanoyltransferase Not determined. Not determined. Not determined. f3-15mer phage display library (X15) 793 PHCVPRDLSWLDLEANMCLP(18) VTCGSIAEYGWLDLAAACSS(1) PRCMQTSYWMDGLQPESCKG(1) RVCAAPESRLFRGMPLGCDD(1) 4 Phage display (common panning) 3 PMID:10493820 14-3-3 protein theta Not determined. Not determined. Not determined. X2CX14CX2 phage display library In fact, microtiter wells were coated overnight with GST-14-3-3τ in TBS.', '3', '10493820', 'PHCVPRDLSWLDLEANMCLP efficiently blocked the binding of 14-3-3 to the kinase Raf-1, a physiological ligand of 14-3-3, and effectively abolished the protective role of 14-3-3 against phosphatase-induced inactivation of Raf-1. PHCVPRDLSWLDLEANMCLP efficiently blocked the binding of 14-3-3 to the kinase Raf-1, a physiological ligand of 14-3-3, and effectively abolished the protective role of 14-3-3 against phosphatase-induced inactivation of Raf-1. 794 RNCWGNIPLTSSSVERLCDAR(1) DACSKQGMGVLLSGWPGPCTT(1) PACLLRSEEYVVECGGDVGLE(1) VCCGVNESLSRSAHADSALMR(1) EVCHMPVSCGPTERSLGGESL(1) 5 Phage display (common panning) 3 PMID:10493820 14-3-3 protein theta Not determined. Not determined. Not determined. X2CX18 phage display library In fact, microtiter wells were coated overnight with GST-14-3-3τ in TBS. 795 NCPLNYTYTYRQ HPPDYNVIYRTP DSPLNYTYTYRQ EAPLQYSYSYRA YQPFNYSVLYRA APQTYQTSYRSP MHWPVDYSSTYR RFPVSYTQSYRT WLHPLSYTEAYR VNQPVDYSSTYR SNRPVEYTHVYR APVSYDSHYRGR VNQPSNYTQIYR SNRPLDYSHVYR YSPVTYTKYRLP 15 Phage display (common panning) 3 PMID:10353843 Anti-human SP-A monoclonal antibody PE10 Pulmonary surfactant-associated protein A1 Not determined. Not determined. Ph.D.-12 phage display library (X12) Phage selected by mAbs displayed the consensus peptide sequences that are nearly identical to 184TPVNYTNWYRG194 of human SP-A. 796 SFPPDYWRHYRM CQIPHSYGGYRS VLPHTYSQYRL SALSYTTVYRSP HQQQQDNSSYRQ GSTPQHYTQLYR SPIPHSYGGHLR YRSPPNRWIQYR QNPESFHEYRTN IPHAPRSYVTRY TGPLHYSSQNRH YYPLDYSRHYRM YPSPPNRLIQYR DHPPWYTWLYR TGTPPFQCASYR NQEPGNYASTYR 16 Phage display (common panning) 3 PMID:10353843 Anti-human SP-A monoclonal antibody PC6 Pulmonary surfactant-associated protein A1 Not determined. Not determined. Ph.D.-12 phage display library (X12) Phage selected by mAbs displayed the consensus peptide sequences that are nearly identical to 184TPVNYTNWYRG194 of human SP-A. 797 SDLVSSRML(2) ELFSTKMDI(2) DDELHTSRY(2) EDLETSIWR(1) RDLLTMRML(1) NDLLTSRVL(1) MDLYSQRIL(1) YWDLTSSKL(1) MMDLDTEYL(1) GTDLESYKL(1) ELKTTKYQD(1) EELSTSKWL(1) IDMVMSKMV(1) SLQTFKLMP(1) ETLTTSKWR(1) SNLETSRFY(1) EGLDTWPMR(1) GTEIMTQYL(1) RTSLETWAI(1) GFLDAWLGE(1) 20 Phage display (common panning) 3 PMID:10029604 Human neutrophil cells Not determined. Not determined. Not determined. J404 phage display library (X9) Freshly isolated PMN were incubated with 20 μL aliquots of phage library in 1 mL HBSS containing 0.1% bovine serum albumin (phage buffer) for 1 hour at 20°C in a 1.5 mL microcentrifuge tube. PMN were then washed five times with phage buffer and bound phage were eluted for 5 minutes in 2 mL of 0.1 mol/L glycine (pH 2.2). 798 DLETSFMKV(3)[NT] DLVTSKLQV(1)[0.76 ± 0.02] DLETSFMPA(1)[NT] DLQTEKYMG(1)[NT] DLMTSKYMA(1)[NT] DLETKFMRQ(1)[NT] KDLLTTKLS(1)[NT] KDLQTRAMH(1)[NT] KDLATSKLL(1)[NT] RDLSTRPLW(1)[NT] RDLLTSKYV(1)[NT] TDLQTMAMR(1)[NT] NLDLHTEKI(1)[NT] RAGDLTTTK(1)[NT] ELNTWKMDW(1)[NT] DPESSFMKV(1)[NT] NPRLETYAM(1)[NT] RALSPPPMS(1)[NT] SSRLETSYL(1)[NT] ENLSNWQIS(1)[NT] 20 Phage display (common panning) 3 PMID:10029604 Human neutrophil cells Not determined. Not determined. Not determined. J404 phage display library (X9) ELISA Purified phage clones were incubated in microtiter plates coated with PMN freshly isolated from peripheral venous blood. Bound phages were detected with polyclonal anti-phage antibody and quantified by light absorbance. Optical density values, OD (405 nm), are representative of one of two experiments showing the mean ± SD of quadruplicate determinations. NT represents not tested. Freshly isolated PMN were incubated with 20 μL aliquots of phage library in 1 mL HBSS containing 0.1% bovine serum albumin (phage buffer) for 2 hours at 4°C in a 1.5 mL microcentrifuge tube. PMN were then washed five times with phage buffer and bound phage were eluted for 5 minutes in 2 mL of 0.1 mol/L glycine (pH 2.2) followed by addition of phage buffer containing 0.5% Tween 20 to the remaining cell pellet. 799 FGPNLTGRW(3)[1.16 ± 0.04] DDLSTTRLP(3)[NT] GTDLESYKL(3)[NT] ADLMTKAMH(1)[NT] TPDLETSRL(1)[NT] SNLATSKLL(1)[NT] QWGLQTYKM(1)[NT] SWELLTEAM(1)[NT] SPSLQTFKM(1)[NT] KMDWTTVYK(1)[NT] GAPNLTGRW(1)[NT] GGPNLTGRW(1)[NT] GWLDDMWKG(1)[NT] TLSPWSVWR(1)[NT] APDWWNIWS(1)[NT] WNRIDAWDS(1)[NT] 16 Phage display (common panning) 3 PMID:10029604 Human neutrophil cells Not determined. Not determined. Not determined. J404 phage display library (X9) ELISA Purified phage clones were incubated in microtiter plates coated with PMN freshly isolated from peripheral venous blood. Bound phages were detected with polyclonal anti-phage antibody and quantified by light absorbance. Optical density values, OD (405 nm), are representative of one of two experiments showing the mean ± SD of quadruplicate determinations. NT represents not tested. Freshly isolated PMN were incubated with 20 μL aliquots of phage library in 1 mL HBSS containing 0.1% bovine serum albumin (phage buffer) for 1 hour at 20°C in a 1.5 mL microcentrifuge tube. PMN were then washed five times with phage buffer and bound phage were eluted for 5 minutes in 2 mL of 0.1 mol/L glycine (pH 2.2). 800 DLVTSKLQV(4)[[0.76 ± 0.02]] NDLLTSKMP(4)[NT] DLSTSKLQI(2)[NT] DLPTSKMNL(2)[NT] DLETSFMKV(2)[NT] ADLAMSKWR(2)[NT] LHFADVWK(2)[NT] DLNTYKMSL(1)[NT] DLAKSKLML(1)[NT] DDLVTQKMA(1)[NT] KDLFSWQLI(1)[NT] KDLVTQKYF(1)[NT] EDLSTQRIK(1)[NT] EMDWMKIWR(1)[NT] 14 Phage display (common panning) 3 PMID:10029604 Human neutrophil cells Not determined. Not determined. Not determined. J404 phage display library (X9) ELISA Purified phage clones were incubated in microtiter plates coated with PMN freshly isolated from peripheral venous blood. Bound phages were detected with polyclonal anti-phage antibody and quantified by light absorbance. Optical density values, OD (405 nm), are representative of one of two experiments showing the mean ± SD of quadruplicate determinations. NT represents not tested. Freshly isolated PMN were incubated with 20 μL aliquots of phage library in 1 mL HBSS containing 0.1% bovine serum albumin (phage buffer) for for 1 hour at 20°C in a 1.5 mL microcentrifuge tube. PMN were then washed five times with phage buffer and bound phage were eluted for 5 minutes in 2 mL of 0.1 mol/L glycine (pH 2.2) followed by addition of phage buffer containing 0.5% Tween 20 to the remaining cell pellet.