751 WYGRRW(6) GDYTLF(4) KRRQEP(3) RYFTYP(2) ACDRYV(1) AMDLFT(1) VQYVWT(1) VKNSGP(1) 8 Phage display (common panning) 3 PMID:10967104 GAP-C-SH2 domain Not determined. Not determined. Not determined. f3-6mer phage display library (X6) The TrpE-GAP-C-SH2 fusion proteins immobilized using anti-TrpE antibodies and protein A-Sepharose beads. The sequence GDYTLF is selected by each of the SH2 domains of PLC 752 GDYTLF(5) RILRFY(4) GSGSWG(1) SAVRII(1) SAVNVI(1) SATSRS(1) REAYVF(1) FAAALI(1) KYMPCG(1) ETELTY(1) HGVGSV(1) GVAVTT(1) GLVSNL(1) 13 Phage display (common panning) 3 PMID:10967104 GAP-N+C-SH2 (includes SH3 domain) Not determined. Not determined. Not determined. f3-6mer phage display library (X6) The TrpE-GAP-SH2 fusion proteins immobilized using anti-TrpE antibodies and protein A-Sepharose beads. The sequence GDYTLF is selected by each of the SH2 domains of PLC 753 RGDLKQLSELTW RGDLAALSAPPV SHARGDLVGLTP ILARGDLSTLSA NARGDLGTLPPR RGDLPHQYQHPS RGDLQYPRSLTL RGDLMLPFWPRL RGDLAAPHRTGA RGDLNNYLLMML RGDLPPVPMARE RGDLPQERHYPR RGDLLHASARAS RGDAPSPNIFRL VAPRGDRPTLPI RGDYYANLANWL HARGDFSWLMPE RGDFAQLLITWQ RGDIPSMSTLPR RTDLDSLRTYTL GDLDLLKLRLTR GDLHSLRQLLSR RTDLHTLSSTRH RDDLHMLRLQLW SSDLHALKKRYG TSDLETLRLRLH RDLETLYNRYAT RHDLSELIRRTT HPRTDLASLAKR QRSDLASLKLTL VTDLQSLYWRIK RDDLQTLLNRLG RSDLDTLLARHQ QSGDLYTLRNSR RSDLTALTQLLL KGDLATLIRTNL QSAHRGDIPNVL ASDISALSARLR RDFQYLWNRVSQ VSDVYQLTERLR AGDLVTFKLRLH HHELDSLRARLM RGD, D-L-x(2)-L 42 Phage display (common panning) 3 PMID:9890954 Integrin alpha-V-beta-6, integrin αVβ6 Fibronectin, FN Not determined. Not determined. Ph.D.-12 phage display library (X12) RTDLDSLRTYTL, selected from the phage sequences was a selective inhibitor of RGD-dependent ligand binding to αvβ6 in isolated receptor assays (IC50 = 20 nM), and in cell adhesion assays (IC50 = 50 mM). DLXXL peptides were highly specific inhibitors of αvβ6-fibronectin interaction as synthetic scrambled or reversed DLXXL peptides were inactive. 754 RTDLSML RGDLHSL RGDLYTL RGDLLHT RGDLTSQ RGDLPPS RGDLPPY RGDLPHS RGDLPSP RGDLYVG RGDIPPL RGDILPR RGDMQMP RGDMLTH RGDHLSH RGDAYPS RGDAMPP RGDTSAL LLRGDMS LLRGDMA RGD 20 Phage display (common panning) 3 PMID:9890954 Integrin alpha-V-beta-6, integrin αVβ6 Fibronectin, FN Not determined. Not determined. Ph.D.-7 phage display library (X7) 755 RGDALTILPPML RGDIYFHLAPRN RGDLDNYPGATM RGDSMGPFTVKM RGDSFDTRYAPL ARGDGLLFHLSG TRGDHFMPPWWG VRGDALPDPAIG IPRGDHYLWPQI AMRGDTYLHPTK WLQRGDGFLQLP YGPARGDHFRWG AFNIRGDHWVPD TEISPRGDNFIN NTHAPRGDGYPA GIVTCRGDLFCS DGRSYSTKYWFM NGRLDHPSSPGQ GRIFHTIGPSER GRMPFPNFSPHG GRFPFIASALPP RGD 21 Phage display (common panning) 3 PMID:9890954 Integrin alpha-V-beta-3, integrin αvβ3 Fibronectin, FN Not determined. Not determined. Ph.D.-12 phage display library (X12) 756 GAHWQFNALTVR(13)[1.15 ± 0.13] TSYGRPALLPAA(1)[0.36 ± 0.31] MDHLAPTFRPAI(1)[0.27 ± 0.27] TLRAIWPMWMSS(1)[0.22 ± 0.12] IPLTANYQGDFT(1)[NT] 5 Phage display (common panning) 4 PMID:10993908 Hyaluronan, HA Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Binding assay Uncoated paramagnetic beads (Dynal) were labeled with 2 mg/ml HA. To test binding, 125I labeled peptides in 3% BSA in PBS were incubated with the HA-coated beads, and then counted for radioactivities. HA-specific binding was determined by subtracting the baseline cpm that bound to noncoated beads from the cpm associated with the HA-coated beads. Specific binding was then calculated from the specific activity (~3.45e7 cpm/μg) and the number of beads added to the assay. Data shown were reproduced from the graph. NT denotes not tested. GAHWQFNALTVR showed specific binding to soluble, immobilized, and cell-associated forms of HA, and it inhibited leukocyte adhesion to HA substrates almost completely. 757 LQAKKKRPK(3) VIAKIKKPK(3) VEAKGHKKK(2) KWKTPKVRV(1) AIAKKHKWN(1) KWKLHGHIK(1) GKGPKWMR(1) 7 Phage display (common panning) 3 PMID:11070036 Transferrin receptor protein 1 Iron-loaded human transferrin Not determined. Not determined. X9 phage display library Bound phage were eluted with low-acid buffer (0.1 M glycine, pH 2.2). 758 GHKAKGPRK(3) KDKIKMDKK(1) 2 Phage display (competitive panning) 3 PMID:11070036 Transferrin receptor protein 1 Iron-loaded human transferrin Not determined. Not determined. X9 phage display library Bound phage were eluted with ligand (iron-loaded human transferrin; Sigma-Aldrich) in TBS buffer (50 mM Tris-Cl, pH 7.5; 150 mM NaCl). 759 IEAYAKKRK(2)[1.78] GHKVKRPKG(2)[1.94] KNKIPKSPK(1)[NT] 3 Phage display (competitive panning) 3 PMID:11070036 Transferrin receptor protein 1 Iron-loaded human transferrin Not determined. Not determined. X9 phage display library ELISA Absorbance at 490 nm was measured. Data shown were repeoduced from the graph and expressed as the means ± SEM. NT denotes not tested. Bound phage were eluted with low-acid buffer (0.1 M glycine, pH 2.2) and ligand (iron-loaded human transferrin; Sigma-Aldrich) in TBS buffer (50 mM Tris-Cl, pH 7.5; 150 mM NaCl). 760 FDDARL FSDARL FSDMRL FVDVRL FTDIRL FNDYRL FSDTRL 7 Phage display (common panning) 3/4 PMID:10583225 M protein from patient 3 Not determined. Not determined. Not determined. f3-6mer phage display library (X6) 761 DPAFIFYHSTLFFNS GGHDGDPVLTGTLFY AVDPRMFYLLLRGGA 3 Phage display (common panning) 3/4 PMID:10583225 M protein from patient 3 Not determined. Not determined. Not determined. X15 phage display library 762 PIHYIF YIHYIF RIHYIF WREWFL WWAMKP 5 Phage display (common panning) 3/4 PMID:10583225 M protein from patient 6 Not determined. Not determined. Not determined. f3-6mer phage display library (X6) 763 LILSSGELLRHPRGA 1 Phage display (common panning) 3/4 PMID:10583225 M protein from patient 6 Not determined. Not determined. Not determined. X15 phage display library 764 QGAAPFS(1) SLRDTPL(1) LPSYQAI(1) QNPHSPR(1) LNYHRPS(1) LTNHGTA(1) 6 Phage display (common panning) 1 PMID:11839467 Hhydroxyethyl radical-modified bovine serum albumin, BSA-HER Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 765 FGNPGAA(1) WAMSPTK(1) NIDNPNK(1) FHENWPS(1) GQLPSSP(1) YNLPWLN(1) 6 Phage display (common panning) 2 PMID:11839467 Hhydroxyethyl radical-modified bovine serum albumin, BSA-HER Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 766 FHENWPS(15) 1 Phage display (common panning) 3 PMID:11839467 Hhydroxyethyl radical-modified bovine serum albumin, BSA-HER Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 767 SPRPPYP(1) SLMANLI(1) LQPTYSL(1) IPSIPTM(1) 4 Phage display (common panning) 1 PMID:11839467 Acetaldehyde-modified bovine serum albumin, BSA-AcH Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 768 NMERVPV(2) ASFAYTT(1) TPTTTRS(1) WPTMLSK(1) SLPPNWP(1) 5 Phage display (common panning) 2 PMID:11839467 Acetaldehyde-modified bovine serum albumin, BSA-AcH Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 769 GPRAYHE(2) YGTTASL(2) LRHPTLP(1) IGLPLRS(1) WTWPPAY(1) SPDSRLR(1) AAPMALF(1) LPAPPFR(1) TPQTWPL(1) DVELRPL(1) TNNKPIF(1) 11 Phage display (common panning) 3 PMID:11839467 Acetaldehyde-modified bovine serum albumin, BSA-AcH Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 770 IMFESN(2)[ND, ND] IMFQSA(1)[ND, ND] NYFVQG(1)[ND, ND] NYFVET(1)[ND, ND] LFTEYG(1)[0.0027 ± 0.0007, 0.00090 ± 0.00007] NYLVTS(1)[0.036 ± 0.008, 0.024 ± 0.006] NMLVYS(1)[0.16 ± 0.03, 0.032 ± 0.003] VYHVST(1)[0.031 ± 0.003, 0.04 ± 0.01] VFVETS(1)[0.9 ± 0.2, 0.10 ± 0.02] IFLETS(1)[1.3 ± 0.3, 0.2 ± 0.1] VYLATD(1)[0.028 ± 0.005, ND] VFVVNG(1)[0.03 ± 0.01, ND] VFTEER(1)[NT, NT] LTMVQE(1)[NT, NT] QDFPMY(1)[NT, NT] VMFQTD(1)[NT, NT] SYVEYQ(1)[NT, NT] LVAFAN(1)[NT, NT] VHFISR(1)[NT, NT] NSVMIA(1)[NT, NT] FIGVSY(1)[NT, NT] VHVEYT(1)[NT, NT] MWFEAP(1)[NT, NT] VFYSRE(1)[NT, NT] EMFFPV(1)[NT, NT] AFMTRG(1)[NT, NT] IYLVEN(1)[NT, NT] VFVEMP(1)[NT, NT] LYYVTE(1)[NT, NT] WVMTEA(1)[NT, NT] IYYSVS(1)[NT, NT] LMEVSK(1)[NT, NT] VLFVSS(1)[NT, NT] YLEVSS(1)[NT, NT] YVVVSN(1)[NT, NT] NMVMFG(1)[NT, NT] GLNMPA(1)[NT, NT] NFAAFS(1)[NT, NT] FAMAEA(1)[NT, NT] INFESG(1)[NT, NT] LVLQEG(1)[NT, NT] VMPTMS(1)[NT, NT] AYLIQG(1)[NT, NT] TFQVQ(1)[NT, NT] 44 Phage display (common panning) 1-5 PMID:10964781 HIV-1 protease, HIV-1 PR Not determined. Not determined. Not determined. fTC-LIB-N6 phage display library Binding assay The kcat (1/s) and Km (μM) values of peptides with sufficient solubility to conduct kinetic measurements were determined at the pH used in the phage selections, pH 6.7, and additionally at pH 5.6, which is closer to the pH used in many substrate and inhibitor studies of HIV-1 protease. Data were shown as Kcat/Km (1/μMs). ND represents not determined, NT not tested. 771 PIGLRS(4) RPYGLL(3) RPAPLM(2) PALRSR(1) PAGIYA(1) HPLPLS(1) LPRPML(1) PTPAVT(1) GPRPLK(1) PSPDPL(1) PVPLPL(1) KTLPQA(1) SPYPLR(1) KPFLRG(1) PRPFLR(1) RALPPS(1) GPGPGL(1) PRALRA(1) PLPLVR(1) P-x-[GP]-L 19 Phage display (common panning) 4 PMID:10600499 Matrix metalloproteinase-14, MMP-14 proMMP-2 Not determined. Not determined. fUSE-His-N6 phage display library At round 4 selection, the phage were initially immobilized to Ni-NTA and substrate phage were released by proteolytic cleavage. This technique was adopted to prevent an accumulation of "nonreactive" phage, which do not interact with Ni-NTA even before treatment with protease. 772 SRLPSCKRSYCSVAH(6) SRLVGYCTRSPAVCR(5) TFTRVVTDVYRGRLS(3) TWPVVHGACRAHGHC(2) TLIPRSFCPTHDRDC(2) VSLFYTSHVAVRCCN(2) VRLPGKLLRSCERGF(1) RASWLLAFVKRAPFS(1) SAVGFWKSRLAGAYF(1) TRVMFDRGITHHCCH(1) TFRVVCSLPRAGCTAH(1) LCMVRNLLTSPLHHA(1) VWWRALLVFATLYSV(1) MSVWVTHNRVESWFA(1) SSIRISYTARFSGFL(1) 15 Phage display (common panning) 4 PMID:9761687 Protein Rev Not determined. Not determined. Not determined. f3-15mer phage display library (X15) 773 SRLPSCKRSYCSVAH(17) TLIPRSFCPTHDRDC(4) WRISGGLVYFMRYAS(2) SRLVGYCTRSPAVCR(2) TFTRVVTDVYRGRLS(2) SRLPSYNHFFILGSH(1) SHLVRGSFGAGVLVR(1) TRVMFDRGITHHCCH(1) 8 Phage display (common panning) 5 PMID:9761687 Protein Rev Not determined. Not determined. Not determined. f3-15mer phage display library (X15) 774 FLPRGDDYPRR[1.66] VQGRGDSHPAI[1.37] HPCRGDCLA[1.02] KPDRGDYAP[0.91] TTARGDSDK[1.57] ILDRGDSYY[NT] 6 Phage display (common panning) 3 PMID:10869561 Integrin alpha-V-beta-3, integrin αvβ3 Cytotoxic T-lymphocyte protein 4 Not determined. Not determined. CTLA-4 (X3-RGD-X3) scaffold library ELISA Absorbance at 450 nm was measured. Data shown were reproduced from the graph. NT denotes not tested. 775 CPWWDDPQDIC CRWWQETDDSC CEADDSSPGRC CNIYAELDDSC CEYSDSGIEVC CALSENKLRVC CAQSENARPVC CHSSENRSSC CFPSAFSENQC CLPFSENRTC CPTSENRAHSC CLPSPLLENRC 12 Phage display (common panning) 3 PMID:9671121 Anti-BLG polyclonal antibody Beta-lactoglobulin, Beta-LG Not determined. Not determined. pVIII-9aa.Cys phage display library (CX9C)