51 Alpha-CT phage display library (XCCX3CX5C) 13 5.0e8 J. K. Scott (Simon Fraser University, Burnaby, Canada) Semi-random NNK Circular The library is based on vector f88-4 (GenBank Accession AF218363). 52 LX8 and X15CX phage display library pool 12, 17 J. K. Scott (Simon Fraser University, Burnaby, Canada) Semi-random NNK 53 X15 and X21 phage display library pool 15, 21 Anthony J. Conley Completely random NNK Linear 54 CX12C phage display library 12 Tel-Aviv University (TAU) Completely random Circular The library is based on the fth-1 expression vector in which random peptides are recombinantly fused to the NH2 terminus of a recombinant protein VIII of the fd filamentous bacteriophage. 55 f3-6mer and J404-3 phage display library pool 6, 9 Completely random NNK Linear Library f3-6mer (GenBank Accession AF246446) is based on vector fUSE5, which displys its guest peptides on all five copies of the minor coat protein pIII at one tip of the virion. The library is also known as fUSE/6mer. 56 CX6C phage display library 6 Igor Fisch Completely random Circular 57 X6 phage display library 6 Igor Fisch Completely random Linear 58 X4[RK]X3 phage display library 7 Richard L. Stevens Semi-random NNN Linear Phage display peptide library is tryptase specific. The genome of the Ff bacteriophage consists of the 11 genes designated gI to gXI. Although the protein (pIII) encoded by gIII is chymotrypsin-, thermolysin-, and subtilisin-susceptible, it is trypsin-resistant. Thus, phage display peptide libraries can give insight into the substrate specificities of certain proteases. 59 L100 phage display library (X10) 10 6.0e8 Matthew D. Scharff (Einstein College, New York) Completely random NNK Linear L100 is based on fUSE5. 60 CX9C phage display library 9 C. Richter King Completely random Circular The library was constructed to contain a variable 9-amino acid peptide flanked by cysteine residues inserted into the GeneIII protein of the phage fUSE5. 61 S-(X)5-FRW-(X)4 phage display library 9 1.0e5 Jarl E. S. Wikberg Semi-random Linear A small library was constructed to test this phage display system using a partially randomized oligonucleotide (MS-5pcr) that kept the MSH-core sequence Phe7-Arg8-Trp9, which is believed to be most essential for receptor binding, to direct phage binding toward the receptors. The Ser at the junction with the pelB leader sequence was also maintained to reduce the amount of sequences that may not be processed by the leader peptidase. 62 Cys6 phage display library (CX6C) 6 2.2e7 T. E. Michaelsen Completely random Circular The library present peptides of six random amino acids constrained by flanking cysteines were created by insertion of double-stranded oligonucleotides between the Sfi1 site of the vector fUSE5, providing the gene 3 protein (g3p) final amino acid sequences NH2-ADGACX6CGAA-g3p for the hexamer library (cys6), where X stands for any amino acid. 63 Cys9 phage display library (CX9C) 9 5.5e7 T. E. Michaelsen Completely random Circular The library present peptides of nine random amino acids constrained by flanking cysteines were created by insertion of double-stranded oligonucleotides between the Sfi1 site of the vector fUSE5, providing the gene 3 protein (g3p) final amino acid sequences ADGACX9CGAA-g3p for the nanomer library (cys9), where X stands for any amino acid. 64 X9 phage display library 9 Heimo Breiteneder Completely random Linear Random peptides are displayed on filamentous phage as fusion to the NH2 terminus of the major coat protein pVIII. 65 X15 phage display library 15 Michael Jaye Completely random NNK Linear A fusion phage display library was constructed in the filamentous bacteriophage fuse 5B, a vector derived from fd filamentous phage. The fuse5B vector was constructed from the vector fuse5 by removal of an existing BstXI site in fuse5 followed by replacement of the SfiI cloning sites with BstXI cloning sites. 66 GX6G phage display library 6 2.5e9 Zoller and Smith Completely random NNK Linear 67 X6PPIP phage display library 6 2.0e9 Zoller and Smith Completely random NNK Linear 68 RSLRPLX6 phage display library 6 5.8e8 Zoller and Smith Completely random NNK Linear 69 PPPYPPX6 phage display library 6 3.1e8 Zoller and Smith Completely random NNK Linear 70 RKRSHRXXPPPXXXVQ phage display library 5 Christian Freund Semi-random Linear Sequences of the focused library RKRSHRXXPPPXXXVQ were cloned into PC89. PC89 was a gift from Gianni Cesareni(Dipartimento di Biologia, Universita di Roma). 71 X9 phage display library 9 Gianni Cesareni(Dipartimento di Biologia, Universita di Roma) Completely random Linear 72 X10 phage display library 10 1.0e8 Daniel Baty Completely random NNK Linear 73 X12 phage display library 12 Peptide Door, Japan Completely random Linear 74 X6 phage display library 6 1.0e7 5.4e8 Michael N. Margolies Completely random Linear A bacteriophage-displayed library contains randomized mutations at H chain residues 30-35 of the anti-digoxin antibody 26-10 Fab. 75 AA-xxxxx-AA phage display library 5 2.0e6 James A. Wells Completely random Linear