51 MMELGRV QVWPSSH VNPTYGN 3 Phage display (common panning) 3 PMID:15684661 Anti-omp40 monoclonal antibody Pg-ompA1 Outer membrane protein 40, Omp40 Not determined. Not determined. Ph.D.-7 phage display library (X7) The minimal epitope requirements of the MAb and the predicted amino acid sequences were identified in the region of 96IALDQTLGIP105 in 40-kDa OMP. 52 GDCFFGFLNSPWRVC RSSYWVYSPWRFISR SPWR 2 Phage display (common panning) 3 PMID:12855711 Anti-vWF monoclonal antibody 82D6A3 von Willebrand factor (vWF) 2ADF, Not determined. f3-15mer phage display library (X15) 53 CMTSPWRC CRTSPWRC CYRSPWRC SPWR 3 Phage display (common panning) 4 PMID:12855711 Anti-vWF monoclonal antibody 82D6A3 von Willebrand factor (vWF) 2ADF, Not determined. CX6C phage display library 54 ECTRWSNRSRCF(13) QCHTWSNRRSCL(13) RCHAWSNRKSCV(8) KCGRWSNRSSCT(8) KCEPDDPWPQCI(7) QCGRWSNRSYCS(4) ACTTWSNRSKCP(4) NCGKWTNRRTCL(3) CGRWFNRSDLHC(3) KCSRWTNRHLCD(2) KCTRWTNRHLCS(2) KCTRWTNRAHCP(2) TCHRWGNRTSCQ(2) SCHAWSNRRTCR(2) ACHEWSNRSTCT(2) ACKRNHRWGACV(2) CMKWSNRSSRWC(1) QCSKWVNRSRCA(1) CSKWHNRSKRHC(1) CSKWANRLVSIC(1) QCSRWSNRTSCT(1) QCHRWANRISCS(1) RCTQWTNRAYCP(1) ACTQWSNRHMCG(1) KCGPWSNRSSCT(1) TCHPFSNRSTCT(1) ECGSHAWGRRCK(1) W-x-N-R 27 Phage display (common panning) 3 PMID:12676786 Anti-coagulation factor VIII monoclonal antibody BO2C11 Coagulation factor VIII 1IQD, Not determined. X15+XCX8CX+X9+CX10C phage display library pool 55 LDSMHFPFHSRSFWP NLSCTHPLGSPPPAP GQICYYGRDAYLCFL CESSLCLMYSLGPPA QTPPCPIEHCPSFYQ QSTCLSHPLLCLSWN PNCWVGLTGAHSCFL THSVPVAYPWPDLNA SPLDYECISHATVCF YSTPSSILDTHPLYK TLPPPCLSSPSRCVN RTMHPSDEFLPLGMP GTGLVPLFDPRYRFL SSSRQEPYPLYPLFS HPKVGEGIDFTSIVP ATDLLAAYPLYSPSL VVPLGRCVSHPAICA GFPCLSVASACYGIT 18 Phage display (common panning) 3 PMID:16630634 Anti-spike glycoprotein monoclonal antibody 80R Spike glycoprotein 2GHW, Not determined. f3-15mer and f88-15mer phage display library pool 56 RSGGCVGGQYCLTPTH NDWPCLSHTTVCNGTQ ATMPCLSHPSVCKHLY PMHECLSAPSVCADNY TELACLSEAYICDRSN ETFTCISAPWTCVTWL EKMACLSTLDVCMENP NNMSCLSHETICGRNP LPFECISKREVCDTPM SVDDCRWNLNCEPPP SEVYCPRPDRCLRAP VQRDCRWTFSCATLI TPPRCSDQMYCSLSR THQFCPDPKHCLAQP RMPPCMNAGECPTIA DTPDCXGNEKCLEYA TSNFCPAGGPCSPHG NPRVCMNKWECEQAI GPPLGCLSLSCYDVA WNDYCTMNQCDTHN KPLHCGDTFCSLNQ YLEHCTMNECLNAR NGYHCLSEFCMPHP SMEECRLWLCPPYE YKPWCEMNKCKPLA VMPECLSRLCDFDM DDMPGCYPMCTLNK YDSYCIMNFCGHAA YTAADCPGLLYLCP NDVRCKLWLCPMPD NNWPCLNETCPTKG VQWPCLSKQCNDNI YQADCLMNRCPTAE SAPECHLYYCPEQA ANPVCRLWMCPPIV RQTEPCNLWFCPQV REPPCVQVHCSTAK PKEQPWSEFRPAGM ADCTLWFCPQTSN CLSATCDCTLCGP FPELTCWTCLASS PPAYSCLCPWAHM 42 Phage display (common panning) 3 PMID:16630634 Anti-spike glycoprotein monoclonal antibody 80R Spike glycoprotein 2GHW, Not determined. f88-Cys1 phage display library (X5CXCX5) 57 TCLWSDLRAQCI REKRWIFSDLTHTCI WSD 2 Phage display (common panning) 4 PMID:16940148 Anti-gp120 monoclonal antibody b12 Surface protein gp120 (SU) 2NY7, Not determined. LX8 and X15CX phage display library pool 58 HSFGDLSISPNSLTAWPGTL WLMRAAWPWDYT NLRSTSFFELWAKWP GEFPDWDNLPLLCEG GRPQWYLQFEDYWRS DSLRVDEHHEVMVPM DWVPFFGFFFSRLQLP NWPRWWEEFVDKHSS VRFHLAGWLPAVVSFVIFSDH DGLRSDGSWLARFVFNGSGFY GNFTASTASWGDELLALY FVWEEHVGFLMRN NWPRWEEFVDKHSS FAHEGSASFRLSSKVEDWVSR CVFYANVEEEVQCWL FCVRLMCSGLIPFFVLCCFFA FSRADFFPLSYSLSSVPSTAL FSPYPPDIVHTTAFSSFVNPVD SSFFYSALTPSFPSPYSQSSR RFFAFPMVCASFFLVAIAFFP PLPPSRFFITVCLTFLFSLSFF VRPCVASLLLFFCLLFLLLPS ICFPFNTRYCIFAMMVSSLVF QQAGSYPGCIDYYYCHASAIG CLPAYGCKSFREPF RHFVPLYLSVSYDGFSRGASI PIIHPHPPRIAMRVSISPFP TPTDSTVRGSSTMDGFLKSVY TASFWRSVSFPWVLSFLAFSH GAQVNRKCAWHPRHI VAKKLWVPQVSGSNF HWGVYR 32 Phage display (common panning) 3 PMID:16940148 Anti-gp120 monoclonal antibody b12 Surface protein gp120 (SU) 2NY7, Not determined. X15 and X21 phage display library pool 59 CWVWSDEYASLMGC CRVWSDATGGYISC CRVWSDEHSAQMAC CERVWSDEHENYTC CRVWSDEWNSYGDC CILWSDINDRFISC CLWSDLLSQYTKPC CQILWSDVRGKFEC CPWMWSDILDRAIC CMWSDYLNRC CMWSDLYDELVNC CLYSDLRGTYETPC CTQIYSDDLQRWTC CTLVYSDYVGDTIC CLWDDLYGTYAEGC CKLWNDESARYTGC CTLWDDELGRSTHC CLPAFLYDDREHPC CLYNVDTGTRSEYC WSD 19 Phage display (common panning) PMID:16940148 Anti-gp120 monoclonal antibody b12 Surface protein gp120 (SU) 2NY7, Not determined. CX12C phage display library 60 HQARPPWTV(3) QLTDAIRQH(3) HHRHSPDNL(3) SAWGRNWHN(3) LGQRTPKSY(2) LTVQARPVG(1) SFQWRPWHI(1) WHQNRGGQA(1) TIQQXPAL(1) SHKQTGPG(1) HWNQPRWSS(1) HQQLPTRWD(1) FHLGTWRPG(1) VQAHGWGRP(1) YHKRPVGTP(1) LVQRYDHRT(1) DWRTPLQQH(1) RSLNLQDFN(1) RFSVETQI(1) ASAEKAHRR(1) ATGQYRGHS(1) TRHDYRQVL(1) VLSYRWDHW(1) FVGYRPAGW(1) HYRTLGDHR(1) GKVHHRNSS(1) AQGGRNQHW(1) MRNVTERHW(1) TSMLRNTSS(1) KRNWMNHGF(1) HSNSRSAQV(1) SNGHSVRQM(1) TSDRLLTIK(1) 33 Phage display (common panning) 3 PMID:7592831 Cytochrome b-245 Neutrophil cytosol factor 1, NCF-1 1WLP, Not determined. J404-3 phage display library (X9) 61 MFSPWNDHF(3) RVRQFG(2) VRHDLLF(2) VRPARHWWK(2) DLTRQG(2) SSVHYM(2) KGAPWQEHE(2) STRVRWGAY(1) ATRVPRKHW(1) TRVMRE(1) TRPRWHKHA(1) KRHRRKTWG(1) LRVWNL(1) WHKHWTRPV(1) WHKTWRVKG(1) KVLPDVRAA(1) VRAVTL(1) VRPPGG(1) SAVRIL(1) ARLAVR(1) ARSPRQIHI(1) ILRQAN(1) NRHIRRL(1) VYGGSRQWG(1) STRVRRQL(1) ALRDLLYPD(1) KFDLLSPFS(1) LGDLLKDMY(1) WHDLGF(1) WDFSDLHW(1) DVSWDLITA(1) AARVDPLVK(1) VPARFDLDN(1) PGGDLY(1) TFDLTS(1) SSGPWKEHF(1) ESGAFA(1) FRIGHF(1) GSHFSIFS(1) PALPWAEHF(1) VHIMKL(1) PWSEHF(1) 42 Phage display (common panning) 3 PMID:7624379 Neutrophil cytosol factor 1 Cytochrome b-245 heavy chain Not determined. Not determined. f3-6mer and J404-3 phage display library pool 62 GGPPRV(1) FTKKIYGPP(1) YLDGGP(1) HGMKFPGPA(1) TQQRGP(1) LMVKPREKHW(1) KWHKNHPP(1) FTWPRW(1) AHKHWRPRT(1) 9 Phage display (common panning) 3 PMID:7624379 Neutrophil cytosol factor 1 Cytochrome b-245 light chain 1WLP, Not determined. f3-6mer and J404-3 phage display library pool 63 CPSSVRHSC(7) CSLRPLEIC(5) CKATNMHMC(2) CRATPEPGC(2) CSSANHQTC(2) CESNKPWYC(2) CPYKGPSEC(2) CILTDMPHC(2) CWKTPTGKC(1) CYLAHTAKC(1) CHSVAAKWC(1) CISSGRPLC(1) CNMSSLTIC(1) CKSLNDRHC(1) CQPRSILAC(1) CSTTRLQHC(1) CGRLPQGVC(1) CTWASKLMC(1) CQNNDSSMC(1) 19 Phage display (competitive panning) 3 PMID:12639821 CD8+ T cell line Monoclonal antibody 4B4 Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Peptides were eluted from a T cell line by 4B4 mAb. 64 MTAPPRN(13) NTSSQRP(3) ACPYKGP(2) GPVNPLS(2) FTRVPAN(2) FSAYRIG(2) FSPPPNT(1) AARPPQT(1) AARMPGL(1) MAPHPLI(1) MSSPNHA(1) MMNSPPA(1) MTRSPPA(1) LSRTVPA(1) TASIWRA(1) SYSPSPP(1) QLSITRL(1) 17 Phage display (competitive panning) 3 PMID:12639821 CD8+ T cell line Monoclonal antibody 4B4 Not determined. Not determined. Ph.D.-7 phage display library (X7) Peptides were eluted from a T cell line by 4B4 mAb. 65 SLSSRQSP(8) CTSSRPSG(1) SGFGRLSD(1) RSQTRKSK(1) KKQGRDST(1) RKQKRRTE(1) PPSFRRSS(1) LPTGRATT(1) NTPTKLSP(1) RRPTKKNT(1) RGEKRSKS(1) MLLIRTWE(1) VTYARLCY(1) LSYRKLRF(1) GTRRREEH(1) DRKGRQQQ(1) RYPCRYGL(1) KEENRKNN(1) FHPSRHPP(1) IAREKGQQ(1) ICPPRLLQ(1) 21 Phage display (common panning) 2 PMID:9395536 Tryptase Fibrinogen alpha chain Not determined. Not determined. X4[RK]X3 phage display library 66 SLSSRQSP(12) 1 Phage display (common panning) 4 PMID:9395536 Tryptase Fibrinogen alpha chain Not determined. Not determined. X4[RK]X3 phage display library 67 WRAFLPRWHA(1)[0.22] WNRGWRWWMG(1)[0.18] GFFWKWRIGR(1)[0.16] HIRWKGHISW(1)[0.09] [HKR](2)-[HKRFYW](2)-H 4 Phage display (common panning) 3 PMID:9858597 Cryptococcus neoformans melanin Not determined. Not determined. Not determined. L100 phage display library (X10) ELISA Absorbance at 405 nm was measured with a Ceres 900HDi (Bio-Tek Instruments Inc., Winooski, Vt.). Melanin-binding peptides were characterized by a high proportion of positively charged and aromatic residues. 68 RWPKRHLSGH(2)[0.73] LHKLVRHGRW(1)[1.94] YERKFWHGRH(1)[1.67] YLRRHTHVFW(1)[1.65] KKHSHYWVRY(1)[1.63] EFGTRHMRHR(1)[1.47] YRHHAHGGRG(1)[1.24] RKKWHGWTRW(1)[1.18] PKWRHGYTRF(1)[1.12] RHGTVKHARH(1)[0.94] RRHWHPPVQI(1)[0.90] EAYKRRWHWP(1)[0.86] SRVPFRHYHH(1)[0.62] RRPEHTKARW(1)[0.44] [HKR](2)-[HKRFYW](2)-H 14 Phage display (common panning) 4 PMID:9858597 Cryptococcus neoformans melanin Not determined. Not determined. Not determined. L100 phage display library (X10) ELISA Absorbance at 405 nm was measured with a Ceres 900HDi (Bio-Tek Instruments Inc., Winooski, Vt.). Melanin-binding peptides were characterized by a high proportion of positively charged and aromatic residues. 69 CELYENVGMYC(18)[0.49, 10-25] 1 Phage display (common panning) 3 PMID:9360978 Growth factor receptor-bound protein 2 Receptor tyrosine-protein kinase erbB-2 Not determined. Not determined. CX9C phage display library ELISA,Surface plasmon resonance (SPR) The G1 phage is attached to the plate surface and GST-Grb2 domain is allowed to bind at 3 M of protein concentration. Binding was detected using anti-GST antibodies. Absorbance at 405 nm was measured. Data shown were reproduced from the graph. Besides, the IC50 values are determined by mixing peptide with recombinant Grb2 or Grb2 SH2 domains and then measuring the amount of binding at equilibrium to the immobilized SHC phosphopeptide, biotin-DDPSpYVNVQ. Synthetic G1 peptide blocks Grb2 SH2 domain association (IC50 10–25 μM) with the SHC phosphopeptide. In fact, the recombinant GST-Grb2 protein is target. 70 RSANAI[4700] RNAQVR[3200] RKASLS[1010] RSATRD[740] SGRARQ[NT] SKSGRS[NT] SSRNAD[NT] TARLRG[NT] TARSDN[NT] TSRMGT[NT] TSRQAQ[NT] TTRRNK[NT] TTSRRS[NT] WSGRSG[NT] AIKRSA[NT] GRRGNR[NT] GRSVNN[NT] HTRRMK[NT] ISTARM[NT] KAADVT[NT] KKRTND[NT] KMSARI[NT] KRRDVA[NT] KRVSKN[NT] KSADAA[NT] RAAAMV[NT] RAGNIR[NT] RAHRDN[NT] RARDDR[NT] RARHMV[NT] RARSPR[NT] RAVGHQ[NT] RAVVDS[NT] RGGKGP[NT] RGRSAV[NT] RGVDMN[NT] RGVKMH[NT] RHRSDI[NT] RKGQGG[NT] RKLHMN[NT] RKMDMG[NT] RKMDRS[NT] RKMRMG[NT] RKNQRV[NT] RKQRDS[NT] RKRVGA[NT] RKSKVV[NT] RKSTSS[NT] RKVGSL[NT] RKVPGS[NT] RKWISG[NT] RLATKA[NT] RMRKND[NT] RNAVEP[NT] RNDRLN[NT] RNGKSR[NT] RNMPLL[NT] RNTGSH[NT] RRMTMG[NT] RRRLNM[NT] RRTLDF[NT] RRAVSN[NT] RSAKVD[NT] RSAVVK[NT] RSDQFL[NT] RSDNPN[NT] RSERSL[NT] RSGDPG[NT] RSGNTT[NT] RSGNMG[NT] RSNGVG[NT] RSPDGM[NT] RSRRLP[NT] RSRVTS[NT] RSSHSS[NT] RSSOAA[NT] RSSSSH[NT] RSSSTV[NT] RSTDLG[NT] RSTNVE[NT] RSTRHK[NT] RSYTNS[NT] RTSPST[NT] RTSVNL[NT] SKRASI[NT] SQTCVR[NT] TERRVR[NT] TQRSTG[NT] TRRDRI[NT] VARNYK[NT] VSRRNM[NT] SGRSA 90 Phage display (common panning) PMID:9252355 Urokinase-type plasminogen activator Not determined. Not determined. Not determined. X6 fAFF1-tether C phage display library Dot blot,Binding assay The dot blot analysis of the digestion of all 100 substrate phages was performed by u-PA using a wide variety of stringencies of digestion (data not shown). Although this semiquantitative assay cannot provide kinetic constants, it can provide an accurate rank ordering of the lability of the substrate phage clones. Under the most stringent conditions examined, 11 of the 100 substrate phage, containing eight distinct randomized hexamer sequences (RKASLS, RNAQVR, RRAVSN, RSAKVD, RSANAI, RSATRD, RSAVVK and RSSSSH), proved to be particularly labile u-PA substrates. Besides, four peptides (RSANAI, RNAQVR, RSATRD and RKASLS) were chosen for detailed kinetic analysis and compared with the hydrolysis of a control peptide containing the P3–P4' sequence of plasminogen. All four of the selected peptides were substantially improved substrates for u-PA, by factors of 840–5300, compared with the control, plasminogen peptide (kcat/Km, 0.88 1/Ms). Data shown were kcat/Km (1/Ms). NT denotes not tested. 71 SVTVVPFRWYSCS[43000.0 ± 12600.0] SLDFNSFRWCSAL[15900.0 ± 4070.0] SSLECSFRWGPEH[3700.0 ± 580.0] SSIISHFRWGLCD[7.6 ± 2.4] 4 Phage display (common panning) 3 PMID:9346944 Melanocyte-stimulating hormone receptor, MSH-R Melanotropin alpha, Alpha-MSH Not determined. Not determined. S-(X)5-FRW-(X)4 phage display library Binding assay All peptides were synthesized as amides, and affinities to the hMC1 receptor were characterized in standard ligand binding assays with the insect cell. Ki (nm) values for the different peptides on the MC1 receptor are measured and shown. 72 CQDPICFCGADGACYCTSRNC(12/16)[1.22 ± 0.04] CAWHYRFCGAAHSADGACREVFLVC(2/16)[0.32 ± 0.02] 2 Phage display (common panning) 2-3 PMID:16423833 Polymeric immunoglobulin receptor Not determined. Not determined. Not determined. Cys6 phage display library (CX6C) ELISA Absorbance was read at 405 nm (Tecan Sunrise Microplate Reader; Tecan Austria Gesellschaft, Salzburg, Austria) with Magellan 3.0 software. 73 CVVWMGFQQVC(6/13)[1.89 ± 0.07] CWTSGARWRLC(1/13)[0.33 ± 0.01] 2 Phage display (common panning) 2-3 PMID:16423833 Polymeric immunoglobulin receptor Not determined. Not determined. Not determined. Cys9 phage display library (CX9C) Absorbance was read at 405 nm (Tecan Sunrise Microplate Reader; Tecan Austria Gesellschaft, Salzburg, Austria) with Magellan 3.0 software. This study implied that the C9A peptide (CVVWMGFQQVC) sequence may be exploited for pIgR-mediated epithelial transport without interfering with secretory immunity. 74 CVVWMGFQQVC(13/35)[1.26 ± 0.03] CALVSEAGCLVWAA(12/35)[0.70 ± 0.06] CIIVPHAYAWC(6/35)[0.31 ± 0.03] 3 Phage display (common panning) 1-2 PMID:16423833 Polymeric immunoglobulin receptor Not determined. Not determined. Not determined. Cys9 phage display library (CX9C) ELISA Absorbance was read at 405 nm (Tecan Sunrise Microplate Reader; Tecan Austria Gesellschaft, Salzburg, Austria) with Magellan 3.0 software. This study implied that the C9A peptide (CVVWMGFQQVC) sequence may be exploited for pIgR-mediated epithelial transport without interfering with secretory immunity. 75 CPYGAALHC CYGGATLLC CQPAVANTC CSYLNVMHC CTGPLPNRC CNPTPEKRC CKPSSPPFC 7 Phage display (common panning) PMID:16928679 Protein tonB Iron(3+)-hydroxamate-binding protein fhuD Not determined. Not determined. Ph.D.-C7C phage display library (CX7C)