701 LHYSYPVADP QGQSYPDXVV PWSYPPVXES AVAYPVVDXD SVSYPSE AVAYPVVED PFAYPVXPPP PPVSVAYPEP PWSYPVQESP LAPAYPLPPD LHYSYPVTGP GSRAYPGAAI IYSYPVTEQQ PLAYPLPQSE 14 Phage display (common panning) 3 PMID:15882053 Anti-AMA-1 monoclonal antibody 5G8 Apical membrane antigen 1 Not determined. Not determined. Min-23-R10 phage display library 702 RLHGSWWPYP LPYLAWLPSQ RLQELWDVHT RIPAAWFSSA RLEAAWLPLP 5 Phage display (common panning) 4 PMID:15882053 Anti-apical membrane protein 1 monoclone antibody 1F9 Apical membrane antigen 1 Not determined. Not determined. Min-23-R10 phage display library 703 VSPSWWRGPL RLPPLPLWPG 2 Phage display (common panning) 4 PMID:15882053 Apical membrane antigen 1 Not determined. Not determined. Not determined. Min-23-R10 phage display library 704 QFHVAFWWPA ASVWWLGPVR MYTLWPSYGR SFEFFPRFGF RFAKRWPPVR LQVWWLGPVR 6 Phage display (common panning) 4 PMID:15882053 Mitochondrial import receptor subunit TOM70 Not determined. Not determined. Not determined. Min-23-R10 phage display library 705 IKPVFGVQA IHPLFGFRPS VHPVFAIPWG ARPFFGMFEG YSGITMLPYN VGWPYVYSKG 6 Phage display (common panning) 4 PMID:15882053 Anti-Nef antibody Protein Nef Not determined. Not determined. Min-23-R10 phage display library 706 TQVLSFVPWK 1 Phage display (common panning) 4 PMID:15882053 Protein Nef Not determined. Not determined. Not determined. Min-23-R10 phage display library 707 LGPTCL SDHLCL 2 Phage display (common panning) 3 PMID:15896002 1,3-diketones 1-BSA and 2-BSA Not determined. Not determined. Not determined. YLK-(NNK)6 phage display library 708 SPFLGQ 1 Phage display (common panning) 3 PMID:15896002 1,3-diketones 1-BSA and 2-BSA Not determined. Not determined. Not determined. (NNK)6-YLK Library 709 LHLHRRQMRP QHRRRSRLLMQKLPN RSRRMTRRARARAA RRTTHSPSTKLRPYP PSRRKQ SRRSPYY QRRLTRTA TSSRRLIRLMRRTQY TPRNIMLRRPHPRRP IRRNPS PPKTRRRRQRNTKNT RRISTN RRQQ RRIIP RRHNTTTY 15 Phage display (common panning) 2 PMID:15944412 Phosphatidylserine, PS Not determined. Not determined. Not determined. f3-15mer phage display library (X15) 15 clones encoded peptides that contained contiguous arginine residues. Unlike other Ca2+-dependent PS-binding proteins, these peptides did not require Ca2+ for binding to PS, and the addition of Ca2+ did not alter the phospholipid specificity. 710 HSLKNSMLTVMA(10) SPHTTPMQMLAH(2) 2 Phage display (common panning) 4 PMID:16041565 Tom20 missing residues 2-28 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) One had partial identity (SMLTVMA) with a bacterial signal peptide from Toho-1, a periplasmic protein. The other had partial identity with a mitochondrial inner membrane glutamate carrier. The bacterial signal peptide could carry a protein into mitochondria both in vivo and in vitro. The first six residues of the sequence, SMLTVM, were necessary for import but the two adjacent arginine residues in the 30-amino-acid leader were not critical for import. The signal peptides of Escherichia coli β-lactamase and Bacillsus subtilis lipase could not carry proteins into mitochondria. Presumably, the Toho-1 leader can adopt a structure compatible for recognition by the import apparatus. 711 HYTYWWL(10) 1 Phage display (competitive panning) 3 PMID:16196489 [PA63]7 complex Protective antigen, PA Not determined. Not determined. Ph.D.-7 phage display library (X7) Non-interacting phage were washed away, and then PA was added to elute phage that specifically bound PA. The series of truncated peptide mutants was used to identify a minimal peptide sequence, TYWWLD, that can be used to develop potent polyvalent inhibitors of anthrax toxin. 712 SSPWMRE(7)[2.325, 3.5] ELWRPTR(6)[2.659, 10] ARPHLSF(6)[2.474, 6.2] TLHLSPA(3)[2.763, 5.4] QLQKYPS(2)[2.615, 8.5] QTMTYSR(2)[2.546, 5.0] NLQEFLF(2)[1.988, 0.69] AAQTSTP(1)[1.912, 4.9] GIRHTNR(1)[2.156, 2.4] RPTR 9 Phage display (common panning) 5 PMID:16201785 Isotactic poly(methyl methacrylate), st-PMMA Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA The absorbance data were converted to affinity relative to that of a phage library for st-PMMA. Besides, the apparent affinity constants (Kapp, e11 1/M) were obtained. All data shown were reproduced from the graph. The paper report for the first time a peptide motif that specifically binds to a stereoregular polymer, isotactic (it) poly(methyl methacrylate) (PMMA). 713 HSKYPLPPLPSL(11) AHHHWHPLPTLP(5) HPGYPLPPFPLP(1) HTWHPLPILPPK(1) H-x-W-[YH]-P-L-P(2)-L-P 4 Phage display (common panning) PMID:16274251 SH3 domain of tyrosine-protein kinase Lck Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) In fact, the target is glutathione s-transferase (GST)-SH3 fusion proteins. 714 CTKNSYLMC(3) CLSSSLSDC(3) CKNSLTMAC(2) CTTTDLRAC(2) CPVSLQALC(2) CSSTTPNAC(1) CNSTKYNQC(1) CTNTMLPQC(1) CDRHNLTFC(1) CPPSKMSQC(1) CQPALQMKC(1) CIPTHPRLC(1) 12 Phage display (subtractive panning) 4 PMID:16763842 HUVECs co-cultured with gastric cancer cells Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Immortal gastric epithelium mucosa cells GES and wild-type HUVECs were used as the absorber cells for whole-cell subtractive screening. 715 VYCVL FLFGLFFFTCRS FRIVTCATCVILKAFMGWHYHISRSRS CDSFARSCVRGVGIMKACGRTRVTS LFTDMALSGKVLVKACGRTRVTS LFLCCMGFHGPL LLLSHVSLVCRL CFLLSFVVLGYD 8 Phage display (common panning) 3 PMID:17979224 Paclitaxel (Taxol) Transcriptional repressor NF-X1 Not determined. Not determined. X12 T7 phage display library Among them, two phage clones included the same consensus amino acid sequence (KACGRTRVTS). Analysis of the protein database using BLAST revealed that a portion of this sequence is conserved in the zinc finger domain of human NFX1. 716 HDIYPRH(1) THRLLL(1) MPHIHRH(1) HPRPRNN(1) QGHIGNE(1) SPPQSRA(1) APPXSPT(1) YPXPPLX(1) RQGGQYP(1) QRATPFP(1) 10 Phage display (common panning) 3 PMID:17630779 Anti-N domain of human ACE monoclonal antibody 5F1 Angiotensin-converting enzyme, ACE Not determined. Not determined. Ph.D.-7 phage display library (X7) All sequences fall into two groups. Alignment of these sequences with each other and full-size ACE falls within amino acid residues 1256-1265 (in the cytoplasmic tail of ACE), whereas alignment with the N domain alone reveals similarity with amino acid residues 471-483. 717 QPHTLSMHRHYY(2) SPSDRLMHNHYH(2) TPPTSRAHAHYY(2) RNHQTHRRQDGK(1) FAWXQHLHALEP(1) 5 Phage display (common panning) 3 PMID:17630779 Anti-N domain of human ACE monoclonal antibody 5F1 Angiotensin-converting enzyme, ACE Not determined. Not determined. Ph.D.-12 phage display library (X12) All sequences fall into two groups. Alignment of these sequences with each other and full-size ACE falls within amino acid residues 1256-1265 (in the cytoplasmic tail of ACE), whereas alignment with the N domain alone reveals similarity with amino acid residues 471-483. 718 HITSLLS(6) HTTHREP(5) APASLYN(4) NQDVPLF(3) TTNGYI(3) AYPYPHT(2) NNATLG(2) NSLMRPA(2) QGFGSPL(2) THHPHQK(2) TLARPSV(2) WDPPLRL(2) WHRAPLP(2) 13 Phage display (in vivo) 2-3 PMID:17551506 Wistar Kyoto (WKY) rats' kidney Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Twelve- to thirteen-week-old WKY rats (n =3 rats/phage display round) were infused with 2e11 plaque-forming units (PFU) of PhD 7mer library in round 1, and with 2e11 PFU of amplified phage from the kidney for rounds 2 and 3. APASLYN were found in the kidneys and non-target organs(heart, brain, and lungs). Authors engineered the HI loop of Ad19p to accommodate peptide insertions and clones. Intravenous delivery of each peptide-modified virus resulted in selective renal targeting, with HTTHREP and HITSLLS-targeted viruses selectively transducing tubular epithelium and glomeruli, respectively. 719 CQSHKLPSC(2) CVGNDNSSC(1) CKPETAPLC(1) CKLPVPPHC(1) CEHPPYLQC(1) CTSSDHLQC(1) CIAPTHNLC(1) CKTHHNLIC(1) CPDALPSSC(1) CSTLAFPLC(1) CQSALTGVC(1) CTTLQSAQC(1) CPFSLSALC(1) 13 Phage display (in vivo) 3 PMID:17384922 Tumor endothelial cells, TEC Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Mice (n=4 per round) were injected intravenously with 1e11 PFU of the whole phage library. After 15 min of circulation time, the animals were killed, and the number of phages localizing to TEC formed vessels as well as to murine kidney (control murine tissue) was determined. No such enrichment was seen in the mouse kidneys, suggesting the isolation of specific phages that preferentially bound to human tumor vessels rather than to mouse normal vasculature. 720 CTVALPGGYVRVC(9) 1 Phage display (subtractive panning) 5 PMID:16878978 Human melanoma cell line Not determined. Not determined. Not determined. CXnC phage display library pool (n = 3-12) The highly metastatic melanoma clone Me6652/4 and the low metastatic clone Me6652/56 were chosen as positive and negative cell lines, respectively. A subtractive panning strategy was applied, in which the phage were first adsorbed onto reference cells before the supernatant of this reaction was transferred to the target melanoma cells, Me6652/4. DNA sequencing of the 15 clones revealed that 9 clones were identical. The corresponding predominant peptide sequence was the cyclic 13-mer CTVALPGGYVRVC, Pep42. The cellular receptor for Pep42 was identified as the surface membrane form of glucoseregulated protein 78 (GRP78), a member of the heat shock protein family and a marker on malignant cancer cells. 721 CHKKPSKSC(22) CTKRNNKRC(1) CRRWESKRC(1) 3 Phage display (common panning) 6 PMID:16841905 Silicon dioxide, SiO2 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Two of the phage surface displayed peptides enriched with basic amino acid residues, STB1 (HKKPSKS) and STB2 (TKRNNKR), showed a cross binding affinity to both metal oxides (SiO2, TiO2). 722 CHKKPSKSC(22) CTKRNNKRC(1) CDQQTSEFC(1) 3 Phage display (common panning) 6 PMID:16841905 Titanium dioxide, TiO2 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Two of the phage surface displayed peptides enriched with basic amino acid residues, STB1 (HKKPSKS) and STB2 (TKRNNKR), showed a cross binding affinity to both metal oxides (SiO2, TiO2). 723 RMWPSSTVNLSAGRR(4) RLAYWCFSGLFLLVC(2) PNLDFSPTCSFRFGC(1) GRVPSMFGGHFFFSR(1) PVAAVSFVPYLVKTY(1) 5 Phage display (common panning) 4 PMID:16536485 Complement receptor type 2, Cr2 Complement receptor type 2, Cr2 Not determined. Not determined. f3-15mer phage display library (X15) To select specifically bound phage and exclude nonspecifically bound phage, a stringent biopanning process was performed including several measures: extensive washing with TPBS (pH 7.4), stringent washing with TPBS (pH 5.0), decreasing the incubation time in each round of selection, and elution with sCD21 at the last round selection. Two sequences, PNLDFSPTCSFRFGC and RLAYWCFSGLFLLVC, contained two cysteine residues. The distance between the two cysteines are five and eight amino acid residues, respectively. Consequently, the formation of cyclic peptide structures with constrained conformations is more probable than cross-linking. Except for RMWPSSTVNLSAGRR, all sequences of selected peptides, consisted mostly of hydrophobic residues, which may participate in hydrophobic interactions with the CD21 receptor. However, the five sequences did not show obvious homology with each other. 724 SMSIASPYIALE(18) SMSIGSPYITFG(6) SPGPMKLLKTPL(6) SMSIASPYIPWS(5) TLNINRLILPRT(5) 5 Phage display (subtractive panning) 3 PMID:17043801 Human gastric cancer cells GC9811-P Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Phage selection was performed with the GC9811 cell line used for the depletion. GC9811-P cells were derived from its parental cell line, GC9811. No complete homologies were found with previously determined protein sequences using a GenBank search of short peptide sequences or nucleotide sequences. 725 IVNWNLTAPGPRFES(15) SEVRASERTAHYEH(13) VHHVGYRVPGPRFES(5) MWNMDPIWIAAVES(4) FGSSKGRAPGPRFES(4) WSNPFPGPAGES(3) DPEVAARELGPPVES(2) WWRLDPINVAAGES(2) SQYEDPFNVARRES(1) QLWWDPIGVARVES(1) YEFDPILISRVES(1) VPEVALKECGPPVES(1) QIEVALKELGPPVES(1) AIVTALIELGPPVES(1) AIQRAFQELGPPVES(1) AIRQAWLELGPPVES(1) AIMRSAQELGPPVES(1) WYIGKETHLGPPVES(1) WYIGPENNLGPPVES(1) GVRIDERELGPPVES(1) AVIRAQWELGPPVES(1) EPGRMVEALGPRFES(1) SEVAGFVVSDRW(1) TMRVDERELGPPVES(1) QDRLTRYVGPPVES(1) SLIGSGSWWPAVES(1) LINEATGPAFVES(1) QDITASFILGPPVES(1) 28 Phage display (common panning) 2-3 PMID:15777347 Anti-gp91-phox Monoclonal antibody CL5 Cytochrome b-245 heavy chain Not determined. Not determined. J404 phage display library (X9) Phage display analysis confirmed this specificity, indicating that the CL5 epitope contains the region 135-DPYSVALSELGDR of gp91-phox.