676 TCSKKYPRSPCM(16) 1 Phage display (in vivo) 3 PMID:20223498 Pea aphid guts Not determined. Not determined. Not determined. LX-8 phage display library (XCX8CX) Briefly, aphids were allowed to feed for several hours through parafilm membranes on a sucrose solution containing the f88.4-LX8 phage library. The guts were then dissected, unbound phage washed away, and bound phage removed, amplified and used for another round of feeding. After each round of biopanning, between 100 and 400 phage plaques were recovered from the aphid gut epithelium. After the third round of biopanning, the phage DNA from 16 plaques was extracted, and the DNA sequences encoding the peptides displayed by each phage were determined. TCSKKYPRSPCM bound to the midgut and hindgut of the pea aphid Acyrthosiphon pisum. Binding was confirmed by labeling the aphid gut with a TCSKKYPRSPCM-green fluorescent protein fusion. TCSKKYPRSPCM reduced uptake of Pea enation mosaic virus (Luteoviridae) from the pea aphid gut into the hemocoel. TCSKKYPRSPCM also bound to the gut epithelia of the green peach aphid and the soybean aphid. 677 LALPPLAPNHHH(7) 1 Phage display (common panning) 3 PMID:20300752 Purified serum IgG from ankylosing spondylitis patients Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Seventeen out of twenty randomly selected phage clones exhibited specific reaction with purified sera IgG from ankylosing spondylitis patients, among them seven coming from the same clone. And only this clone's inserted peptide sequence was given in the original paper. 678 GHLHLRVPTLKM EFIRSPHSVDWL SQSRNPPMPPPR RRPPYRVPPKLF SLYERHPASTYP HTVSRRPLPSSG RHTHGNLLRFPP RKPTQSLPTRLV TRRPHKMRSDPL SRQFLHSLDRLP QTPYQARLPAVA RNNLNQTYPERR YSLLPVRPVALT HLHHHLDHRPHR TLTWHTKTPVRP WHPHRHHHLQWD R-x-P 16 Phage display (common panning) 3 PMID:16690883 Gap junction alpha-1 protein Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) After removing the unbound viruses by washing with Tris buffered saline containing 0.5% tween-20 (TBS-T), low affinity binders were eluted with a solution of TBS-containing Cx43CT at a concentration of 100 μg/ml. High affinity binders were then recovered by overlaying the well with a culture of E. coli, to allow the tightly bound phage to infect the bacteria. The paper suggest that RXP-based peptides could serve as tools to help determine the role of Cx43 as a regulator of function in conditions such as ischemia-induced arrhythmias. 679 VSYSELTSYYMR(27) ISWMDLTAYYRG(2) 2 Phage display (subtractive panning) 3 PMID:16041387 Capsid protein p24, CA Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Bovine serum albumin(BSA) was used for negative selection to screen out peptides that target the nucleic acid–binding domain. After three positive and two negative selections, 36 of 41 analyzed phages bound to CA but not to BSA. 680 ITFEDLLDYYGP(15) ISWSELDAFMQM(6) VSYSELTSYYMR(6) STTWQDFFKTFG(5) YNEPWWLTPSMF(3) VKYHDLQTFFDP(1) VTYAQLQAYFPD(1) LEFSDLEDFFRA(1) LNFSDLNNYFLL(1) LDYPWWLSMNNI(1) SYTQWDNAPGTR(1) IADRPRAWIGSP(1) AMKTHTAIAPRA(1) HPQMHATPYQTT(1) SPSNLYEQLLHW(1) LPMIDIYRTAEL(1) 16 Phage display (subtractive panning) 3 PMID:16041387 C-CANC Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) NC was used for negative selection to increase specificity. After three positive and two negative selections, The peptide coding regions from 46 were sequenced and shown to code for 16 different peptides. 681 STFTKSP(0.50) NHWASPR(0.28) 2 Phage display (in vivo) 5 PMID:15536193 Murine bone marrow Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) BALB/c mice 6-8 weeks of age (Taconic Farms, Germantown, NY) were injected intravenously (tail vein) with PDPL (Ph.D-7 Phage Display Peptide Library Kit, New England Biolabs) diluted in 0.25\r\nml Dulbecco's modified Eagle's medium (DMEM). 682 STFTKSP(0.85) 1 Phage display (common panning) 3 PMID:15536193 Murine hematopoietic stem cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Biopanning combined with fluorescence-activated cell sorting (FACS) was repeated three times, yielding a phage clone that attached to primitive\r\nLin-Hoechst/rhodamine stem cells. 683 THRTSTLDYFVI SHKYPLPPYFHW TIKMHTLSYTGL 3 Phage display (common panning) 5 PMID:15895095 Chlorine-doped polypyrrole, PPyCl Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 684 SGHQLLLNKMPN(19) LWATFPPRPPWL(15) WSAAPTKPPYHT(5) ILANDLTAPGPR(4) HHGHSPTSPQVR(3) LPYGTSNRHAPV(3) YVQGWNYHDLTR(3) LWAAFPPQASVA(3) FDTPHTLTWFHG(2) 9 Phage display (subtractive panning) 6 PMID:15319030 Human glioma cell lines Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The library was screened against\r\na mixture of human glioma cell lines including dGli36, SF767, U87MG, U251MG, and U373MG at 37°C to increase the probability of obtaining sequences that could interact generically with most glioma cells. Phages rescued were further subjected to negative panning with nonglioma cell lines A549, CNE2, and\r\nHepG2, to eliminate nonspecific background binding. LWATFPPRPPWL is able to target specifically to tumors of glial origin, which would allow the design of applications related to the diagnosis and treatment of human gliomas. 685 ALPSTSSQMPQL ALPSTSSQMQL ALPSTSSQMPQV YQSSVSVQLPTL TTRQVPVSYTSS RLGFPPQTHAL HQPIQILEQPYT NSQNIGVGSW HVDQRYWFLGAP TTGPNTRHHA MGIAEQLMH 11 Phage display (subtractive panning) 8 PMID:16889372 Undifferentiated P19 embryonic carcinoma stem cells Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) To reduce nonspecific binding of phages to the cell surface, the phage libraries were preadsorbed to the differentiated P19 cells before each selection on the undifferentiated P19 cells. ALPSTSSQMPQL may be targeted to a marker expressed on the stem cell and thus become a practical tool for the isolation of somatic stem cells. 686 SNTQLDQ AAKTWNA HQLTKTL TELLVVT TGGSYGS LPGIPWL TLQPPLL SPNMNLT LTPHPIY NDTRPPR VPLNHSP QHSSSFY TMTWTSQ TTATDYS SAPKTTF 15 Phage display (common panning) 3 PMID:12882541 Quinoprotein glucose dehydrogenase Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 687 NATLTRL(3) VTYRAPA(2) HTLNWTH(1) HTPMLHP(1) QGIAEFR(1) QLHPLKP(1) LGAPFTG(1) 7 Phage display (common panning) 4 PMID:12882541 Quinoprotein glucose dehydrogenase Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 688 NATLTRL(7) SILPYPY(3) 2 Phage display (common panning) 5 PMID:12882541 Quinoprotein glucose dehydrogenase Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 689 NATLTRL(10) 1 Phage display (common panning) 6 PMID:12882541 Quinoprotein glucose dehydrogenase Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 690 LPAPLLPAAPLY(4) SISKSPMSLLSP(1) WAPGSMPTSRLA(1) GNYWSNGHSYHS(1) 4 Phage display (common panning) 4 PMID:12882541 Quinoprotein glucose dehydrogenase Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 691 LPAPLLPAAPLY(9) 1 Phage display (common panning) 5 PMID:12882541 Quinoprotein glucose dehydrogenase Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 692 GETRAPL(5) EYHHYNK(2) AAPMQVT(1) AKPSPFP(1) ALQBKPI(1) AMPYAPR(1) ANMSLLT(1) ANSKLSP(1) APATSIG(1) APQPWLM(1) ASTQQPT(1) DLRIAAS(1) EGLPANP(1) EYTHTPY(1) FPGKQTT(1) GHSHSHS(1) GPGPNIS(1) GPNQVEW(1) GSTQPPW(1) HLHTIGR(1) HPFILKP(1) HPPBVSS(1) HSFPHAP(1) HVLWTPP(1) IRPPSII(1) KLVASNP(1) KVTTTRV(1) LAKHPDS(1) LERGPYG(1) LVPPSGT(1) MGPPSTP(1) MPPGYPH(1) NALKFSA(1) NPFYSLR(1) QLTMFPS(1) QPNNHAH(1) QQQHPFK(1) SAPERFS(1) SFGENSI(1) SGSPPSV(1) SLPDPIH(1) SLRPSID(1) SMPKLIN(1) SNAQSMR(1) SNMAQHR(1) SPIRHVH(1) SPQLPQL(1) SQSPFFP(1) SSHGSLS(1) SSQYAHL(1) SWLPHNA(1) SYMYKPQ(1) TARQDSI(1) TGHHIFY(1) THLSRTP(1) TLSNYSQ(1) TNGLRTA(1) TPPQSTG(1) TPTIHKT(1) TPVQQVA(1) TQEYRSA(1) TQMRQPP(1) TQPPIRT(1) TSPIPPK(1) TTPRFIL(1) TTTLRPS(1) TYATDRR(1) TYSQSMT(1) VKPBTGA(1) VLPRASY(1) VNPVNTH(1) VSAQTRQ(1) WAPPPAG(1) WNLQPPQ(1) WPNTYRL(1) YHPMSSL(1) YLKPPGP(1) 77 Phage display (subtractive panning) 4 PMID:14759804 Human saphenous vein vascular smooth muscle cell Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Following one round of biopanning against target HSVSMC, a preclearing step was introduced whereby biopanning on nontarget cells (HepG2 cells and peripheral blood mononuclear cells) was performed. 693 ATPPPWLLRTAP DGSIHKRNIMPL DYDSLSWRSTLH GEPTTDMRWRNP GLWPWNPVTVLP HMLNDPTPPPYW KPAYTHEYRWLA LETTCASLCYPS LGTDWHSVSYTL LGTLNAGVPGFP LTHSKNPVFLST LVPTTHRHWPVT LVSNARGFNNLS NTRIPEPIRFYM NVYTFHSMSPMP QHTTLTSHPRQY SDFSDTMPHRPS SIDTIQILSLRS SISWASQPPYSL SMVKFPRPLDSR SPTLGASVAQTN TMSPNVYYTAFG TQIPSRPQTPSQ VCSNMYFSCRLS VPPHPMTYSCQY VPRLEATMVPDI VPTKPELPVNFT WSSDLPQPASTY YITPYAHLRGGN NVYTDNTLSPTP 30 Phage display (in vivo) 4 PMID:15139528 Gastrointestinal tract tissue, GIT tissue Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Phage display libraries were screened in vivo within the gastrointestinal tract of a rat model by successive screenings across four cycles of selection. LETTCASLCYPS and VPPHPMTYSCQY were shown to bind to receptors on the surface of human intestinal tissue. 694 FHNHGAA(3) FHNHGKQ(2) RLFTWE(2) SSLPLRK(2) FHNHGIL(1) FHNHGAT(1) FHNHGST(1) FHNHGAP(1) FHGHGLY(1) HIEHLIA(1) HLEHLLF(1) PFFHLIG(1) STPIPAP(1) STFTHPR(1) 14 Phage display (common panning) 3 PMID:15476403 Integrase, IN Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) FHNHGKQ and HLEHLLF exhibit a high affinity for IN and were chemically synthesized. IN. These IN-binding agents could be used as a base for developing new anti-integrase compounds as well as for structural studies of the still unknown three-dimensional structure of the entire integrase molecule. 695 NPRLYE(4) TTYSRFP(3) AEPVAML(2) VPTGYKP(2) ASSRTPS(1) HFWNRPL(1) FHQNWPS(1) HWGMWSY(1) HAWNYIF(1) NSHAIYP(1) SLLSSPQ(1) SPYHTQP(1) LPPNPTN(1) 13 Phage display (common panning) 3 PMID:15476403 Bovine serum albumin (BSA) Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 696 SWALWMLG(14)[NT] FVRWLLLGVP(9)[NT] WLGLMRW(4)[NT] WARWLLLTPA(4)[0.329 ± 0.071] PWLGLWLLGV(1)[0.333 ± 0.058] PWLARWLLGV(1)[NT] LGCWILPRL(1)[NT] DTWARWML(1)[NT] W-L(0,1)-A-R-W-L(2)-[GL] 8 Phage display (common panning) 4 PMID:15672819 Harpin hrpZ Not determined. Not determined. Not determined. Three pools of fUSE5 phage display library ELISA Optical density was quantified at 492 nm. Data for both phages were combined of the results of four assays, each with at least four replicates of the protein sample. Data shown were reproduced from the graph. NT denotes not tested. 697 VPSSGPQDTRTT(4) YSPDPRPWSSRY(3) TLWSQGRSAYPV(2) NNRPEPSPVVPH(1) SPLDGKNIPLGH(1) WPAPAIWHAPTL(1) 6 Phage display (common panning) 3 PMID:15703906 Aluminum Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Most of peptides are rich in hydroxyl-containing amino acids, serine or threonine, or contain histidine. For the aluminum-binding peptides, peptides with a higher number of hydroxyl-containing amino acids bind to the aluminum surface more tightly. The paper suggests an important role for the hydroxyl-containing amino acids in the metal-peptide interaction. 698 ATIHDAFYSAPE(1) NLNPNTASAMHV(1) NLTIASYPSMVV(1) QSHYRHISPAQV(1) QMDISLGRWSSM(1) YMKQIPAGRTNP(1) 6 Phage display (common panning) 3 PMID:15703906 Mild steel Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Most of peptides are rich in hydroxyl-containing amino acids, serine or threonine, or contain histidine. The paper suggests an important role for the hydroxyl-containing amino acids in the metal-peptide interaction. 699 CTVALCNMYFGAKLD(25) GWCDVALCDPLLP(16) GWGDVALCDPLLP(3) PSYCPAPAVSIVAVC(2) V-A-[LV]-C 4 Phage display (common panning) 3 PMID:15789570 Anti-phomopsin A monoclonal antibody C3C11 Phomopsin A Not determined. Not determined. f3-15mer phage display library (X15) The paper suggests that the mimotope peptides bind to mAb C3C11 at the same site as phomopsin A. 700 CGPAEDRVRPWC(7) CNFDRTAPGQWC(5) CGAQMSRVRPAC(5) CSAAVSRTQPWC(4) CDFSRTRAGTWC(3) CGGPNSRVRPWC(2) CGGPDSRTRTWC(2) CEPAVSRTRPWC(1) CDFSRSRPGIWC(1) CEAPESRVVAWC(1) CHAGFGQAWHSC(1) CHPEDRRWLFC(1) CGFYRSGVGQWC(1) CGPDKDRVGPWC(1) CGQHITRVRPWC(1) 15 Phage display (common panning) 3 PMID:15846144 Anti-HMW-MAA monoclonal antibody 225.28S High molecular weight melanoma-mssociated antigen, HMW-MAA Not determined. Not determined. CL10 phage display library (CX10C)