626 TYPGYINHSKAS(2) TIPGYINWRGGP(2) TFFPSTPGPRYT(2) HHTWPGYINYV(1) QGIHPYPGYINH(1) QAPGWINVPPIE(1) NHQYYQVPGSIN(1) THHTSWPGQINS(1) SHHQNMVPGSLN(1) EDYRHSRPGRLN(1) QFPGFYNSSPPY(1) SHTTGVPGSVNH(1) MLPGWYNSLQSR(1) HLNKDANLSFAW(1) YLRAPPQWLTNT(1) P-G-x-[ILYV]-N 15 Phage display (common panning) 3 PMID:19772881 Anti-serine protease pet autotransporter polyclonal antibody IgG Serine protease pet autotransporter Not determined. Not determined. Ph.D.-12 phage display library (X12) The binding reactivities of SHHQNMVPGSLN, EDYRHSRPGRLN and YLRAPPQWLTNT to IgG used for library screening were as weak as control. 627 CTLDDSSTC(2) CSPDDSSTC(1) CAPDDSSTC(1) CLADDSSTC(1) CIDDSSMSC(1) CAHRDDSSC(1) CTYALLSSC(1) CVPNYKNQC(1) D(2)-S(2) 8 Phage display (common panning) 2 PMID:19772881 Anti-serine protease pic autotransporter polyclonal antibody IgG Serine protease pic autotransporter Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The binding reactivities of these phage clones were not determined. 628 CSPDDSSTC(3) CLLDDSSTC(2) CNPDDSSMC(1) CSPDDSSMC(1) CSPDDSSAC(1) CVPDDSSTC(1) CTLDDSSTC(1) CSVDDSSLC(1) [PLV]-D(2)-S(2) 8 Phage display (common panning) 3 PMID:19772881 Anti-serine protease pic autotransporter polyclonal antibody IgG Serine protease pic autotransporter Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The binding reactivity of phage clone bearing CSPDDSSAC was not determined. 629 FDYSKHV(17) AYDTEYV(3) 2 Phage display (common panning) 3 PMID:19811418 Anti-PfMIF monoclonal antibody B9 Macrophage migration inhibitory factor-like protein Not determined. Not determined. Ph.D.-7 phage display library (X7) 630 ESAHHHHWKNFR(11) HWWNTPWAWSHP(6) SPQQLKPWWHSP(3) 3 Phage display (common panning) 3 PMID:19811418 Anti-PfMIF monoclonal antibody B9 Macrophage migration inhibitory factor-like protein Not determined. Not determined. Ph.D.-12 phage display library (X12) 631 HNYESPLLPTSH(2) AEHMIWMSPGRA(1) SHHHRDQMDPKH(1) TMGTTSPQIHRY(1) VSEQHQRYIWSS(1) DAVRPITSSAGN(1) ALSSNHPPAQPT(1) NTRLNNTPDSQL(1) TVMMNKEMLTTR(1) 9 Phage display (competitive panning) 2 PMID:19846220 Anti-lipid-transfer protein polyclonal antibody IgE Non-specific lipid-transfer protein, LTP Not determined. Not determined. Ph.D.-12 phage display library (X12) The target was immobilized on beads via a monoclonal mouse-anti-human IgE. In the second round, binding clones were eluted with Tri a 14. 632 SPHDIKRWTMPH(1) NTSHPPYTHRTM(1) AQKPSLGHLSLT(1) DICTNQCARPTG(1) TATNLLTPRWIT(1) EFTEPHSSQLRD(1) HNKGSPLLPTSH(1) KRTPSGIEPALH(1) NHSFHIIATPAP(1) SFKTHNTWYKLG(1) 10 Phage display (competitive panning) 2 PMID:19846220 Anti-lipid-transfer protein polyclonal antibody IgE Non-specific lipid-transfer protein 1, LTP 1 Not determined. Not determined. Ph.D.-12 phage display library (X12) The target was immobilized on beads via a monoclonal mouse-anti-human IgE. In the second round, binding clones were firstly eluted with Tri a 14 and the non-eluted phages were then incubated with Pru p 3, eluting Pru p 3-specific clones. 633 VWALKSHSHKDI(2) KPEFTRTTHNAP(1) NKNPNMIAQFPN(1) GVSVTSISNIAP(1) HNKESPLLPTSH(1) DNRMFRRLSLRV(1) HNYESPRLPTSH(1) HNYESPLLPTRR(1) HNYESPLLPTSH(1) 9 Phage display (competitive panning) 2 PMID:19846220 Anti-lipid-transfer protein polyclonal antibody IgE Non-specific lipid-transfer protein 1, LTP 1 Not determined. Not determined. Ph.D.-12 phage display library (X12) The target was immobilized on beads via a monoclonal mouse-anti-human IgE. In the second round, binding clones were eluted with Pru p 3. 634 GQSALLSMRHKP(1) ALSLPQTTRALF(1) NDLALQPRNAGP(1) IPLSEFLFADPG(1) HNYESPLLPTSH(1) VMAQTNLFSPRS(1) HNYESPLLLRRM(1) FTTLQPPLLPHQ(1) HQAPQPKPNRLF(1) FTTLQPPLLPXQ(1) 10 Phage display (competitive panning) 2 PMID:19846220 Anti-lipid-transfer protein polyclonal antibody IgE Non-specific lipid-transfer protein, LTP Not determined. Not determined. Ph.D.-12 phage display library (X12) The target was immobilized on beads via a monoclonal mouse-anti-human IgE. In the second round, binding clones were firstly eluted with Pru p 3 and the non-eluted phages were then incubated with Tri a 14, eluting Tri a 14-specific clones. 635 VYPTLHFSARGSG QSQWPVLFFSARG SNIPFQFSARGPG SENLQFSARGPGG HMEGSELSFSARG AHQDETLAFSARG NHPFNALSFSSRG YADLLAQFSSKSG YLPSQISAIGRAG NPVIRYASRSHSG FSARG 10 Phage display (common panning) 3 PMID:20090838 Serine-aspartate repeat-containing protein G Fibrinogen beta chain Not determined. Not determined. Ph.D.-12 phage display library (X12) In fact, the target used in this study was N2 and N3 domain of SdrG (SdrGN2N3). 636 HWRTHHIHHFHQG MSPHHMHHSHGHG AKLAHHIHHFHGG WVPHHIHHFHRAG YTHHHHSWRLHTG AHHPHAWRHSHKG STFHFHTHKARHG HSQHHRFHHTYPG H(2)-[IM]-H(2)-F-H 8 Phage display (common panning) 3 PMID:20090838 Serine-aspartate repeat-containing protein C Neurexin-1-beta Not determined. Not determined. Ph.D.-12 phage display library (X12) In fact, the target used in this study was N2 and N3 domain of SdrC (SdrCN2N3). Fifty randomly picked plaques were selected and the corresponding phages were screened again for binding to SdrCN2N3, to SdrGN2N3 and to BSA as controls. All clones bound to SdrC, however, phage from eight clones displayed significantly higher binding to SdrCN2N3 compared to SdrGN2N3 and BSA. The clone inserts were sequenced. 637 VSGKAGYYSRMH SLTPNSKTRLYA HNPQFNLWHRDT TLAKMGLSPLHG HSSWWLALAKPT HNWHWKHKWMPR HYPWFKARLYPL HYPWSKYPTRSP WHDRLYLKWPHR HLGLYNLLRPWP TSNPHTRHYYPI HMRLSQWPLLKP HYPW, KARLY 12 Phage display (common panning) 3 PMID:20082398 GTPase HRas Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) In fact, the target used in the panning was immobilized GST-H-Ras G12R/A59T-GMPPNP. HYPWFKARLYPL binds Ras in both the active GTP- and inactive GDP-bound conformations with low micromolar dissociation constants. The dodecamer does not alter the intrinsic GTPase activity of Ras, does not compete for Ras binding to the Ras binding domain of Raf, and does not alter cell viability. 638 HSWYPVPIPPSS(12) SVSPTWPLPPFP(2) HLWHLPPRTPTA(1) APGDMLPPLPSL(1) SVSVGMKPSPRK(1) P-x(2)-P 5 Phage display (competitive panning) 5 PMID:20024074 Tyrosine-protein kinase Lck Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) In fact, the target used in the panning was immobilized GST-tagged LckSH3. The sequences of the phage-displayed peptides obtained after five rounds of selection against GST-tagged LckSH3 with the competing GST-tagged SH3 domains from Hck, Src, Pi3k and Abl present in the washing buffer. 639 HPNVLKAPSSNT AETVESCLAKSH VSVWSYQYWRFP KGWMAYPVASSW SSATMTQDSRLI TARNETPAMRMN THPVTDPIAHPP MLPGTSGVTDFQ TFRSNDSYFHQK NLYSNRSAVTSL ASPASHGNNNIS STTVRPHTSDVW 12 Phage display (competitive panning) 2 PMID:20205701 IgE of melon-allergic patients Profilin Not determined. Not determined. Ph.D.-12 phage display library (X12) The target was immobilized on beads via a monoclonal mouse-anti-human IgE. In the second round, binding clones were eluted with recombinant Cuc m 2. All clones were recognized by the serum pool of melon patients in ELISA assays. By contrast, a pool of sera from nonfruit atopic patients recognized none of the selected clones. The peptides included in the selected clones could bind to the specific IgE of melonallergic patients. 640 PFARAPVEHHDVVGL(2) GLDLLGDVRIPVVRR(2) RFLEFPRFFPAIILP(1) PGRLLPGVIQRHFFI(1) GAFSSPRSLTVPLRR(1) CGGLFAGCAALIDVF(1) EFPTFSWSYINDSLL(1) ADWPHARGKFALGNA(1) GFTDVHLHLPGNSHR(1) PVAGMPLFPTAWFAH(1) SAYAATVRGPLSSAS(1) RYASQLSDQILFTLP(1) RDGAFSPPVRWWSFS(1) TGAQAGLHEWRPWGV(1) EDWFSASIRRVPTFA(1) GTWSSTCPLCSATAV(1) PWLPSNLGSRPGLMR(1) 17 Phage display (common panning) 3 PMID:20162339 Brain (brain endothelium) Not determined. Not determined. Not determined. f3-15mer phage display library (X15) In fact, phages were infused via the heart. The major advantage compared to direct infusion into the brain is that the phage have to travel some distance before they reach the brain. On their way, they already encounter endothelial cells: the endothelium of the aorta and carotid arteries. Phage with affinity to ubiquitous endothelial receptors are given the opportunity to bind to these cells and will not reach the brain. This negative selection filters out unspecific binders and allows for the identification of peptides that bind more specifically to brain endothelium. These sequences were deduced from clones isolated from anterior cerebrum. 641 GLDLLGDVRIPVVRR(2) PFARAPVEHHDVVGL(2) GVVNYARAFNVGAAV(1) HAAFEPRGDVRHTLL(1) GDGRFHFLRGFFDSD(1) VRSIALFPPEWSATS(1) GLAHSFSDFARDFVA(1) VTGTQIRLPAYLRFD(1) GYRPVHNIRGHWAPG(1) ITRGGYVIYHDALLA(1) GAYFLSNHAVVRGVG(1) VGRPGGLVGGFASSL(1) LGRAGQSYPSFARGL(1) VVSSRSVLSSQYRGH(1) ALPCNGAGCSRVTAR(1) VPMGLGFLGRGLAPL(1) RSSHHPSFAVSLEPL(1) 17 Phage display (common panning) 3 PMID:20162339 Brain (brain endothelium) Not determined. Not determined. Not determined. f3-15mer phage display library (X15) In fact, phages were infused via the heart. The major advantage compared to direct infusion into the brain is that the phage have to travel some distance before they reach the brain. On their way, they already encounter endothelial cells: the endothelium of the aorta and carotid arteries. Phage with affinity to ubiquitous endothelial receptors are given the opportunity to bind to these cells and will not reach the brain. This negative selection filters out unspecific binders and allows for the identification of peptides that bind more specifically to brain endothelium. These sequences were deduced from clones isolated from posterior cerebrum. Two phage clones, displaying GLAHSFSDFARDFVA and GYRPVHNIRGHWAPG 15 amino acid-peptides (GLA and GYR) that were selected for brain binding in the mouse model, showed significant binding to human brain endothelium (hCMEC/D3), compared to a random control phage. This binding was not seen for other human endothelial cells (HUVEC). Binding to hCMEC/D3 cells was dose dependent. When phage GLA and GYR were individually perfused through the murine brain, their ability to bind to the brain was 6-fold (GLA) and 5-fold (GYR) higher than the control phage. When compared to lung perfusion, phage showed an 8.5-fold (GYR) and 48-fold (GLA) preference for brain over lung compared to the control. 642 GVDQHDNGSRAR(2) WPLHDGGAVNRR(2) FTQLNPTDGGSA(1) TNSFNDSGSAPH(1) H-D-G(2)-S-x-A 4 Phage display (common panning) 3 PMID:19261354 Anti-Toxoplasma gondii tachyzoites monoclonal antibody A4D12 SAG2 protein Not determined. Not determined. Ph.D.-12 phage display library (X12) 643 VQGMLRQDTQNL(1) TSDRDHQNLLAY(1) YNIHEHQNLRAV(1) IIQERQNIWTAP(1) ERSLWQEHQNFP(1) NNWDYPQERQNI(1) AQEHQNLTWRPI(1) DRQTWQNFMLIP(1) QEHQNL 8 Phage display (common panning) 3 PMID:19819255 Anti-chCD132 monoclonal antibody C10 Cytokine receptor common subunit gamma (CD132) Not determined. Not determined. Ph.D.-12 phage display library (X12) 644 KIELPSL(10) KIELPSL 1 Phage display (common panning) 3-4 PMID:19923010 Anti-chicken IL-2 monoclonal antibody 7G5 Interleukin 2 Not determined. Not determined. Ph.D.-7 phage display library (X7) 645 EHLDWFEDWALW(5) FHQQNYNRSIYL(2) EHLDFLDSENTT(1) TYDNDMLYKNHV(1) TGNEKWLYGMLT(1) E-H-L-D-x-N-D-S-L-Y-L 5 Phage display (common panning) 3-4 PMID:19923010 Anti-chicken IL-2 monoclonal antibody 6G5 Interleukin 2 Not determined. Not determined. Ph.D.-12 phage display library (X12) 646 NHLNGSY(8) SILPYPY(1) NHKKGQQ(1) N-H-L-x-G-x-Y 3 Phage display (common panning) 3-4 PMID:19923010 Anti-chicken IL-2 monoclonal antibody 2F9 Interleukin 2 Not determined. Not determined. Ph.D.-7 phage display library (X7) 647 TYWSPTP(4) WHVPFSL(1) TWAWRTP(1) WHPLLSP(1) WHLPRVI(1) WHLPWLE(1) RNVPPML(1) W-H-L-P(2)-SL 7 Phage display (common panning) 3-4 PMID:19923010 Anti-chicken IL-2 monoclonal antibody 7B7 Interleukin 2 Not determined. Not determined. Ph.D.-7 phage display library (X7) 648 YVTKIAS(5) EFNAARL(2) EFNPSNL(2) FKASLLH(1) E-F-K-A-S-x-L 4 Phage display (common panning) 3-4 PMID:19923010 Anti-chicken IL-2 monoclonal antibody 2G11 Interleukin 2 Not determined. Not determined. Ph.D.-7 phage display library (X7) 649 TENPFPE(3) QFNPFPE(2) WENPYPE(2) YYNPYPE(1) TYNPFPE(1) AFNPFPE(1) TENPFPE 6 Phage display (common panning) 3-4 PMID:19923010 Anti-chicken IL-2 monoclonal antibody 3E7 Interleukin 2 Not determined. Not determined. Ph.D.-7 phage display library (X7) 650 PKISGLYADLLAK(7) PGVYINTNRETSS(2) PGPTGIDLWGAEV(1) SGLYL 3 Phage display (common panning) 3-4 PMID:19923010 Anti-chicken IL-2 monoclonal antibody 8H8 Interleukin 2 Not determined. Not determined. Ph.D.-12 phage display library (X12)