26 pVIII-9aa phage display library (X9) 9 F. Felici (Istituto di Ricerche di Biologia Molecolare, Rome, Italy) Completely random NNN Linear The random nonapeptides were inserted into the amino terminus of the pVIII protein coat of bacteriophage fl. 27 pVIII-9aa.Cys phage display library (CX9C) 9 F. Felici and A. Luzzago (Istituto di Ricerche di Biologia Molecolare, Rome, Italy) Completely random NNN Circular Phages is this library contain peptide inserts of 9 random amino acid residues, flanked by two invariant cysteine residues, within the major M13 phage coat protein pVllI. The insert sequences are preceded by AEGEF and followed by GDPAK. 28 pVIII-9aa and pVIII-9aa.Cys phage display library pool 9 F. Felici (Istituto di Ricerche di Biologia Molecolare, Rome, Italy) Completely random NNN pVIII-9aa contained "unconstrained" nonapeptides inserted into the amino terminus of the pVIII coat protein of bacteriophage f1. pVIII-9aa.Cys contained nonapeptides "constrained" by a disulphide bond formed by an additional cysteine at each end of the peptide. Phagemids were propagated using the related helper phage M13KO7 (Promega, Madison, USA). 29 f88-4/15mer and f88-4/Cys4 phage display library pool 14, 15 G. Smith (University of Missouri, Columbia, MO) Semi-random NNK Two phagemid libraries expressing random peptides fused to the N terminus of the pVIII coat protein of M13 phage were mixed in equal proportions. One library (f88-4/15mer) contained linear 15-mer peptides whereas the second (f88-4/Cys4) contained 14-mer peptides with conformation constrained by the presence of cysteine residues within the peptide at positions 5 and 10. 30 X20 fUSE5 phage display library 20 Michael Foley Completely random Linear 31 CX7C fUSE5 phage display library 7 Martin Trepel (University of Freiburg Medical Center, Germany) Completely random NNK Circular The fUSE5 virions were obtained from Dr. G. Smith (University of Missouri, Columbia, MO). 32 CX4C and X6 phage display library pool 4, 6 John C. Reed Completely random 33 CX7C T7 phage display library 7 G. Froman (Department of Medical Microbiology, Uppsala University, Sweden) Completely random NNK Circular 34 X10 phage display library 10 7.2e10 Yipeng Qi Completely random NNK Linear 35 X7+CX7C+CX10C+CX3CX3CX3C phage display library pool 7, 10, 11 Erkki Koivunen (Division of Biochemistry, University of Helsinki, Helsinki, Finland) Semi-random All libraries are based on vector fUSE5. 36 X10 phage display library 10 Erkki Koivunen (Division of Biochemistry, University of Helsinki, Helsinki, Finland) Completely random Linear X10 is based on vector fUSE5. 37 X11 phage display library 11 Erkki Koivunen (Division of Biochemistry, University of Helsinki, Helsinki, Finland) Completely random Linear X11 is based on vector fUSE5. 38 CX6C phage display library 6 Corvas (Gent, Belgium) Completely random Circular 39 X15+XCX8CX+X9+CX10C phage display library pool 9, 10, 12, 15 Claude Granier Semi-random Two M13 phage display library pool were obtained that express either linear 15-mer random peptides (X15) or constrained 12-mer random peptides (XCX8CX) at the surface of the pVIII protein. Two additional phage display library pool, expressing either linear random nonapeptides (X9) or constrained random dodecapeptides (CX10) at the surface of the pIII protein, were obtained. 40 f3-15mer and f88-15mer phage display library pool 15 G. Smith (University of Missouri, Columbia, MO) Completely random NNK Linear Library f3-6mer (GenBank Accession AF246445) is based on vector fUSE5, which displys its guest peptides on all five copies of the minor coat protein pIII at one tip of the virion. The library is also known as fUSE/15mer. Library f88-15mer (GenBank Accession AF246448) is based on vector f88-4, which displays its guest peptides on 100 to 300 copies of the major coat protein pVIII. The library is also known as f88/15mer. 41 J404-3 phage display library (X9) 9 J. B. Burritt Completely random Linear 42 X6 phage display library 6 8.0e8 J. K. Scott (Simon Fraser University, Burnaby, Canada) Completely random NNK Linear The library is based on vector f88-4 (GenBank Accession AF218363). 43 X15 phage display library 15 1.3e9 J. K. Scott (Simon Fraser University, Burnaby, Canada) Completely random NNK Linear The library is based on vector f88-4 (GenBank Accession AF218363). 44 X30 phage display library 30 2.5e8 J. K. Scott (Simon Fraser University, Burnaby, Canada) Completely random NNK Linear The library is based on vector f88-4 (GenBank Accession AF218363). 45 X8CX8 phage display library 17 2.5e8 J. K. Scott (Simon Fraser University, Burnaby, Canada) Semi-random NNK Linear The library is based on vector f88-4 (GenBank Accession AF218363). 46 X15CX phage display library 17 1.2e8 J. K. Scott (Simon Fraser University, Burnaby, Canada) Semi-random NNK Linear The library is based on vector f88-4 (GenBank Accession AF218363). 47 XCX15 phage display library 17 1.0e9 J. K. Scott (Simon Fraser University, Burnaby, Canada) Semi-random NNK Linear The library is based on vector f88-4 (GenBank Accession AF218363). 48 LX-4 phage display library (XCX4CX) 8 2.2e8 J. K. Scott (Simon Fraser University, Burnaby, Canada) Semi-random NNK Circular The library is based on vector f88-4 (GenBank Accession AF218363). 49 LX-6b phage display library (XCX6CX) 10 1.5e8 J. K. Scott (Simon Fraser University, Burnaby, Canada) Semi-random NNK Circular The library is based on vector f88-4 (GenBank Accession AF218363). 50 LX-8 phage display library (XCX8CX) 12 1.5e9 J. K. Scott (Simon Fraser University, Burnaby, Canada) Semi-random NNK Circular The library is based on vector f88-4 (GenBank Accession AF218363).