26 YHWDPLP(2) HPSYPRH(1) HPSDPRH(1) HHRDPCH(1) H-x(2)-D-P-x-H 4 Phage display (common panning) 4 PMID:17424851 Anti-aflatoxin B1 monoclonal antibody Aflatoxin B1 Not determined. Not determined. Ph.D.-7 phage display library (X7) 27 GSHHRHVHSPFV(3) HHPSHFSFSSPA(1) HHTHWPLSARTG(1) HHTHQASFMTRL(1) HHEHYKAVYTTH(1) HHYDTYWVWTTS(1) WAWPTHTHSPPP(1) YKFEYSEGHNLH(1) H-x(2)-H 8 Phage display (common panning) 4 PMID:16704119 Avian infectious bronchitis virus (strain M41) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) One linear peptide "GSHHRHVHSPFV" from the positive phages with the highest neutralization titer was synthesized and this peptide inhibited IBV infection in HeLa as well. 28 CLTPCRNKC(10) 3 Phage display (common panning) 4 PMID:16759988 Anti-arginine kinase monoclonal antibody 38G6 Arginine kinase Not determined. Not determined. CX7C T7 phage display library The peptide has an 83 29 AYQKWQTGA(2) EYQPHQTGL(1) PYENQQTGW(1) GYLPEQLGV(1) PYLFEQSGA(1) RYIALQLGL(1) NYRQDQLGM(1) SYRSLQTGW(1) YYAFGQIGL(1) AYQMYAIPA(1) Y-x(3)-Q-[LI]-G-[ST] 10 Phage display (common panning) 2 PMID:16273596 Anti-cholera toxin peptide 3 (CTP3) monoclonal antibody TE33 Cholera enterotoxin subunit B 1TET, Not determined. pVIII-9aa phage display library (X9) 30 CNQLFNTPPSC(5) CAQTFNSTTEC(1) CRQGLHTPPRC(1) CVRAPLLGPRC(1) CYGPLSSPLNC(1) QLFNTPP 5 Phage display (common panning) 2 PMID:16273596 Anti-cholera toxin peptide 3 (CTP3) monoclonal antibody TE33 Cholera enterotoxin subunit B 1TET, Not determined. pVIII-9aa.Cys phage display library (CX9C) 31 DWVAVKQSYF(2)[0.78/0.686, 5.43/5.08e-9] HLVAVIGSYR(1)[0.782, 7.28e-9] PSLFSWGFGS(1)[0.805, 8.54e-9] V-A-V-x(2)-S-Y 3 Phage display (common panning) 3 PMID:12917476 Shrimp white spot syndrome virus Not determined. Not determined. Not determined. X10 phage display library ELISA Phage clones amplified by panning were tested by ELISA for their ability to bind specifically to WSSV. HRP activity was estimated by measuring A405 using a universal microplate reader (EXL-800; Bio-tek). All samples were tested three times and the mean values are given. Besides the affinity (Kaff) constant of peptides for WSSV was determined according to the method of Beattyet using a solid-phase non-competitive enzyme immunoassay. HLVAVIGSYR had a high specificity and blocked virus infection, with the possible critical motif for virus inhibition being VAVXXSY. 32 VPWMEPAYQRPL[0.36] TLGAQYLPTRTA[0.17] MPPTDYCSLCIK[0.20] PPAHNSKSPMKR[0.29] MPYTNERNPSQP[0.49] TPQLTTGIYPPR[0.58] SLTMMSPPAASD[0.54] LKMPVSELLLKK[0.38] VPLPWHMYAPAR[0.85] 9 Phage display (subtractive panning) 3 PMID:12527505 Osteosarcoma cell line OS-732 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Absorbance at 490 nm was dertemined. Results demonstrated that only 9 clones specifically bound to osteosarcoma cells. Data shown were reproduced from the graph. Osteoblasts were used as the absorber cells for subtraction biopanning from phage display library. 33 SNFASITTPRPH WPTNPTTVPVPS LTSDTYFLPVPA SLHWPVSHPPPP SVSVGMKPSPRP WHSQRLSPVPPA SNQGGSPLPRSV SEPHLPFPVLPH LPLPAPSFHRTT YPLPHPMWSMLP TMTPPPTSVRGT TPLPTIRGDTGT GPPPHHRDYHGP YPAPIKVLLPNS SPYPMALFPLHN SPYPSWSTPAGR P-x-P 16 Phage display (common panning) 3 PMID:12480936 Virion infectivity factor Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 34 ENHSPVNIAHKL(2) ENHSPVNIDHKL(2) ENHSPVNIAHKV(1) EDHSPVNIDHKL(1) ENHYPLHAAHRI(1) ESHQHVHDLVFL(1) TSHHDSHGDHHV(1) PGHHDFVGLHHL(1) 8 Phage display (common panning) 3 PMID:14607870 Anti-ViCPS monoclonal antibody ATVi Capsular polysaccharide Vi antigen (ViCPS) Not determined. Not determined. Ph.D.-12 phage display library (X12) 35 TSHHDSHGLHRV(11) TSHHDSHGVHRV(2) ENHSPVNIAHKL(2) DNHSPVNIAHKL(2) TSHHDSHDLHRV(1) TSHHDYHGLHRV(1) ENHYPVNIAHKL(1) 7 Phage display (common panning) 4 PMID:14607870 Anti-ViCPS monoclonal antibody ATVi Capsular polysaccharide Vi antigen (ViCPS) Not determined. Not determined. Ph.D.-12 phage display library (X12) 36 ENHSPVNIAHKL(3) DNHSPVNIAHKL(1) QNHSPVYIAHKL(1) QNHSPVNIAHKI(1) FPIAALNNETSF(1) QNTDQATPHRML(1) QITDQVNVHHML(1) TNHLGLQSSHRF(1) 8 Phage display (common panning) 3 PMID:14607870 Polyclonal antibody PTS Capsular polysaccharide Vi antigen (ViCPS) Not determined. Not determined. Ph.D.-12 phage display library (X12) 37 TSHHDSHGLHRV(16) ENHSPVNIAHKL(2) 2 Phage display (common panning) 4 PMID:14607870 Polyclonal antibody PTS Capsular polysaccharide Vi antigen (ViCPS) Not determined. Not determined. Ph.D.-12 phage display library (X12) 38 CRELRYLRRAC(17) CPRPLRGVYMC(3) CPRSLRHLANC(2) CLLEKLRMRAC(1) 4 Phage display (common panning) 3 PMID:16361318 Anti-IL-4 monoclonal antibody 11B.11 Interleukin-4 Not determined. Not determined. pVIII-9aa.Cys phage display library (CX9C) The paper suggests that the amino acid residues spanning from 79 to 86 (QRLFRAFR) on IL-4 are of the major binding site for 11B.11. 39 GQQQTPY(1) GLQQASV(1) VQETQRP(1) GLQATPA(1) VTQRLPL(1) TQDTPRT(1) QGRIPTS(1) QSNEPRR(1) IAHHQFP(1) IKTQALM(1) EKHQPER(1) Q-X-[PTS] 11 Phage display (common panning) 2 PMID:17075129 Protein-glutamine gamma-glutamyltransferase 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Database searches showed that several proteins contain peptides similar to the phage-selected sequences, and the N-terminal glutamine-rich domain of SWI1/SNF1-related chromatin remodeling proteins was chosen for detailed analysis. 40 QQQHFLV(1) QQLHLEA(1) QQLSIPP(1) QQSPLWH(1) QQWITVP(1) AQHQLEL(1) WQIPMQL(1) WQTPMNS(1) WQRPYPH(1) SQLWLLP(1) SQLTLLP(1) TQFNPRY(1) FLTPTQP(1) AWPQTSA(1) VPNVHQK(1) Q-X-[PTS] 15 Phage display (common panning) 3 PMID:17075129 Protein-glutamine gamma-glutamyltransferase 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Database searches showed that several proteins contain peptides similar to the phage-selected sequences, and the N-terminal glutamine-rich domain of SWI1/SNF1-related chromatin remodeling proteins was chosen for detailed analysis. 41 CDVPCFGWCVAWC(12) CEGVCRVSCFGWC(3) CDPPCFGWCQDAC(2) CQGGCRVQCFGFC(1) CTGACPVVCFGFC(1) ILCFGMC(1) CDVPCFGWCVSWC(1) CVCFGYCRYEC(1) CHILCFGYCGDLC(1) CEGKCVVSCFGFC(1) C-F-G-[WYF]-C 10 Phage display (common panning) 2-5 PMID:16813566 Plasminogen activator inhibitor 1 Vitronectin 1OC0, Not determined. X7+CX7C+CX10C+CX3CX3CX3C phage display library pool Phage and PAI-1 expressed from HT-1080 cells (100 nM) were incubated in MBS supplemented with BSA and glycerol for 1 h. Formed phage-PAI-1 complexes were captured on polystyrene-adsorbed monoclonal anti-PAI-1 antibody Mab-7 (first and fourth rounds of panning), Mab-5 (second and fifth rounds of panning) or Mab-3 (third round of panning). Individual phage from selection rounds 2-5 were tested for PAI-1-binding in a sandwich ELISA. Sequencing of positives identified a short disulfide-constrained loop CFG(W/Y/F)C as a well-defined consensus motif. A peptide with the sequence DVPCFGWCQDA (referred to as paionin-1 hereafter) was synthesized. The paionin-1 sequence is a chimaera of clones A (CDPPCFGWCQDAC) and B (CDVPCFGWCVAWC) devoid of the flanking cysteine residues, which might be not essential as indicated by the clone G (ILCFGMC). The paionin-1 peptide inhibited the PAI-1 binding of phage clone B, while a linear version paionin-1 with the two cysteine residues replaced by serines had no effect on binding. This suggested that the constraint imposed by the disulfide locked the CFGWC loop in a conformation required for binding or that the cysteines were of contact residues. The binding of paionin-1 to PAI-1 was evaluated further through competition experiments with vitronectin. The results showed that vitronectin significantly inhibited the binding of paionin-1 to PAI-1. Therfore, vitronectin was taken as the template of this set of mimotopes. 42 AARRPFPAPS(19) CFRQGCWVIT(3) SRFKVWWAAG(1) PARRPFPVTA(1) 4 Phage display (common panning) 4 PMID:16527822 Kallikrein-2 Not determined. Not determined. Not determined. X10 phage display library 43 FRTCLRSWACM(6) CYRMPTCMQRD(4) 2 Phage display (common panning) 4 PMID:16527822 Kallikrein-2 Not determined. Not determined. Not determined. X11 phage display library 44 TTSPLSQGSSYI(9) 1 Phage display (subtractive panning) 3 PMID:16369013 Listeria monocytogenes Cation-independent mannose-6-phosphate receptor Not determined. Not determined. Ph.D.-12 phage display library (X12) Panning was performed using Dynex Immunolon microtiter wells coated with L. monocytogenes cocktail (15 serotypes or strains, i.e. 1/2a, 1/2b, 1/2c, 3a, 3b, 3c, 4a, 4b, 4c, 4d, 4a, EGD and three EGD Mutant strains). Nonspecific phages were removed by absorbing the phage library sequentially on uncoated wells and wells coated with BSA. Subtractive selection was carried out by the sequential application of L. innocua cocktail and L. ivanovii cocktail to wells with washing between steps. Low-affinity L. monocytogenes-specific phage peptides were removed by rapid exposure and removal of L. monocytogenes cocktail. The wells were washed before incubation with L. monocytogenes cocktail (30 min) to elute specific high-affinity bound phages. TTSPLSQGSSYI is quite similar to the segment PLAQSGGSSYI in the human cation-independent mannose 6-phosphate receptor (M6PR), which is within the mannose 6-phosphate binding site of the M6PR. Binding of the labeled synthetic PLAQSGGSSYI peptide by Listeria spp. was confirmed by fluorescence spectrophotometry and FACS. Further evidence for binding of the receptor by L. monocytogenes and L. innocua was provided by affinity purification. M6PR is thus indentified as a novel receptor for binding and invasion of Listeria species. 45 HHWTYWLSGTVA FHWPRSWVTWQS SHWWWWDARGYD WHWQWTPWSIQP WHHPWWYPRPGV WHWHPLSWRYST YWPSKHWWWLAP H-W-x-W 7 Phage display (common panning) 3 PMID:16312021 Prostate-specific antigen Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) After three biopannings, 36 clones were randomly isolated and their DNA was sequenced. The deduced amino acid sequences of the corresponding inserts identified 22 different sequences. When tested in an ELISA test for their reactivity, seven clones gave a positive signal with PSA, PSA-ACT but not with ACT, indicating that the recognition was directed against a region of PSA not covered by ACT. 46 VRWGSFAAWL(20) RLNWAWWLSY(5) IPVKWLLRWR(3) LRLQWRAWLA(2) PVRWRWASWL(1) 5 Phage display (common panning) 4 PMID:16524885 Macrophage receptor MARCO Not determined. Not determined. Not determined. X10 phage display library The VRWGSFAAWL phage-sMARCO interaction had significantly slower dissociation kinetics than that between sMARCO and lipopolysaccharide or lipoteichoic acid. Further work with this phage, and the second most enriched phage, displaying the peptide RLNWAWWLSY, demonstrated that both peptides bind to the SRCR domain of MARCO, and that they probably bind to the same site. 47 MDDRGLLLVKKKKHG(24) KKKRHDVEKHIPHVRAS(18) FDGVRKKSRGKSLEY(15) RRGDHDHDIFHWWVH(11) FLDERNYIKKKRHKL(5) SIEVLRGAMHVAPRR(1) GGWYDRKHRRPAPLS(1) KFFRKKSHYHSRTTS(1) 8 Phage display (common panning) 3 PMID:7649995 Heat shock cognate 71 kDa protein BAG family molecular chaperone regulator 1 1HX1, Not determined. X15 phage display library The peptides containing basic sequences with at least KK, KR or RR were enriched. A similar synthetic heptamer NIVRKKK had the highest affinity for Hsc70, and substitution analyses showed that hydrophobic residues followed by basic residues play important roles in maintaining this affnity. 48 CQFDLSTRRLKC(7) CQYNLSSRALKC(2) CVWQRWQKSYVC(1) CMWDRFSRWYKC(1) 4 Phage display (common panning) 3 PMID:16288119 Cetuximab Epidermal growth factor receptor 1YY9, Not determined. CL10 phage display library (CX10C) The in vitro biologic features of mimotope-induced antibodies are similar to those of the monoclonal antibody cetuximab. 49 CQMWAPQWGPDC(51) CKLYWADGEFTC(18) CVDYHYEGAITC(8) CKLYWADGELTC(3) CVDYHYEGTITC(1) 5 Phage display (common panning) 3 PMID:15210798 Trastuzumab Receptor tyrosine-protein kinase erbB-2 1N8Z, Not determined. CL10 phage display library (CX10C) The results indicate that the selected mimotopes are suitable for formulation of a breast cancer vaccine because the resulting Abs show similar biological features as trastuzumab. 50 SSTQVQHTLLQT KVTLHHPPITRS 2 Phage display (common panning) 3 PMID:15684661 Anti-omp40 monoclonal antibody Pg-ompA1 Outer membrane protein 40, Omp40 Not determined. Not determined. Ph.D.-12 phage display library (X12) The minimal epitope requirements of the MAb and the predicted amino acid sequences were identified in the region of 96IALDQTLGIP105 in 40-kDa OMP.