376 SUT12 M13 phage display library 12 M. Yamabhai(Suranaree University of Technology, Thailand) Completely random NNK Linear The phage display library of a random peptide was constructed by cloning DNA inserts assembled from synthetic degenerate oligonucleotides (NN(G/T)12) into an M13 vector, such that the random peptides were expressed as N-terminal fusions to the M13 minor coat protein pIII. The complexity of the library is ∼10e9 members. The titer of the library is ∼10e10 pfu. 377 Ph.D.-12 and Ph.D.-7 phage library pool 7, 12 New England BioLabs, Beverly, MA, USA Completely random NNK Linear 378 X7 and X11 FUSE5 phage display library pool 7, 11 Karyon-CTT Ltd Completely random Linear 379 f88-4 phage display library pool 1.0e8~1.0e9 G. Smith (University of Missouri, Columbia, MO), Bonnycastle Semi-random The peptides were displayed as fusions with the pⅧ coat protein of the f88-4 phage display vector. X6, X15, X30, LX4(XCX4CX), LX6(XCX6CX), LX8(XCX8CX), LX10(XCX10CX), LX12(XCX12CX), Cys3(X5CX3CX4), Cys4(X4CX4CX4), Cys5(X4CX5CX4), Cys6(X4CX6CX4), X8CX8, X15CX and XCCX3CX5C4GIEGRG library were included in the phage display library pool. 380 Ph.D.-7 and Ph.D.-C7C phage display library pool 7 New England BioLabs, Beverly, MA, USA Completely random NNK 381 f5-15mer phage display library (X15) 15 G. Smith (University of Missouri, Columbia, MO) Completely random NNM Linear The phage library was constructed based on the vector fUSE5. It expressed 15-mer random peptides near the N-terminus of phage surface protein pIII. 382 fd-tet X8 phage display library 8 Chuanbin Mao Completely random Linear The landscape phage library contains billions of fd-tet phage clones and in each phage clone the foreign peptide is displayed on the N-terminal end of each copy of major coat protein of fd-tet phage. 383 M13 pVIII phage display library e11 Gregory A. Weiss Semi-random NNS Random peptide sequences CX5C, X2CX4CX2, X4CX10CX4, CX5CX, X2CX5CX2, X5CX8CX5, CX5CX2, X2CX6CX2, X5CX9CX4, XCX5C, X2CX7CX2, X6CX6CX6, X2CX5C, X2CX8CX2, X6CX7CX5, X2CX9CX2, X7CX4CX7, X2CX2CX2, X2CX10CX2, X7CX5CX6, X2CX2CX3, X2CX3CX2, X4CX2GPX4CX4 and X8 were in the phage display library. Random peptide segments (containing both linear and disulfide crosslinks) were expressed on the major M13 coat protein pVIII using a two-plasmid phagemid/helper phage system. 384 Ph.D.-12 phage display and wild type library pool Completely random The phage display 12-mer library is doped with 10% wild type library (i.e. phage that do not express any sequence on their surfaces). 385 T7 X6 phage display library 6 1.5e8 Navneet Sharma Completely random NNK Linear The sequence of synthetic degenerate oligonucleotides inserted in the coding region of T7 phage capsid protein (employing T7 Select 1-1 vector arms, T7 Select system, Novagen, Canada), encoding a random hexamer followed by (His)6 tag is as follows: 59-AAT TCT CTC ACT CCA GGC GGC-(NNK)6-GGT GGT CAT CAC CAT CAC CAT CAC TAA-39 (N represents any nucleotide and K represents T or G). 386 pComb8 CX15C phage display library 17 Department of Biochemistry, University of Amsterdam (Amsterdam, The Netherlands) Completely random Circular 387 pIF15 phage display library 15 Dr. Paolo Monaci (Istituto di Ricerca di Biologia Molecolare, Rome, Italy) Completely random Linear 388 pⅧ and peptides-on-plasmids phage display library pool Semi-random NNK Phagemid libraries displaying random peptides fused to the NH2-terminus of pⅧ were of the form X10, X11, X20, GGCX8C, GGCX10C, and GGCX12C. Libraries displaying random peptides fused to the COOH-terminus of the lac repressor protein were of the form X15, X4CX(4-10)CX4 and X3CX(5-10)CX3. 389 pVIII-9aa.Cys phage display library (CX9C) 11 Dr. A. Nicosia (Institute of Molecular Biology, Pomezia, Rome) Completely random Circular 390 T7 CX9C phage display library 11 Richard G. Smith (University of Nottingham, Cancer Research Laboratories, School of Pharmaceutical Sciences, Nottingham NG7 2RD, UK) Semi-random NNK Circular The T7Select Phage Display System immunology utilizing the T7Select415-1b vector (Novagen, Inc., Madison, WI, USA), was used to display approximately 415 identical copies of each peptide on the capsid head. The peptides were encoded for by a degenerate oligonucleotide insert, consisting of a randomized library of 9-residue peptides flanked by cysteine residues (to impose a structural constraint on the C-terminus of the displayed peptides). These cysteine were also flanked with serine residues, such that the N-terminus of the peptide linked to the capsid head through a serine residue. 391 CX(7-11)C phage display library 7-11 Cwirla SE (Department of Molecular Biology, Affymax Research Institute, Palo Alto, CA, USA.) Completely random NNK Circular The library was constructed on the phagemid vector p8V2. This is a disulfide constrained library expressing peptides of 7-11 random amino acid peptides flanked by two cysteine residues and linked to the N terminus of the major pVIII phage coat protein. 392 Linear-lib and Cyclic-lib M13 phage display library pool 8, 10, 12, 14, 16 Rami N Hannoush (Department of Early Discovery Biochemistry, Genentech, South San Francisco, California, USA) Semi-random NNK Linear Two groups of phage-displayed peptide libraries, the linear peptide library called Linear-lib and cysteine-restrained cyclic library called Cyclic-lib, were constructed by fusing randomized peptides to the N terminus of M13 major coat protein p8. Linear-lib consisted of random peptides with 8, 10, 12, 14, or 16 amino acids, and Cyclic-lib consisted of 14-mer random peptides with varied length between two invariant cysteines. The final diversities for Linear-lib and Cyclic-Lib were 1.8e11 and 7.8e11, respectively. 393 SGTACX2GPX4CSLAGSP phage display library 20 Sidhu SS (Department of Protein Engineering, Genentech, Inc., South San Francisco, California) Semi-random Circular The library consisted of e8-e109 independent transformants. It was displayed on gene 8, under the control of the Ptac promoter. 394 X4CX2GPX4CX4 phage display library 18 Sidhu SS (Department of Protein Engineering, Genentech, Inc., South San Francisco, California) Semi-random Circular The library consisted of e8-e109 independent transformants. It was displayed on gene 8, under the control of the Ptac promoter. 395 X5CX8CX5 phage display library 20 Sidhu SS (Department of Protein Engineering, Genentech, Inc., South San Francisco, California) Semi-random Circular 396 X2CX6CX2 phage display library 12 Sidhu SS (Department of Protein Engineering, Genentech, Inc., South San Francisco, California) Semi-random Circular 397 X5CX9CX4 phage display library 20 Sidhu SS (Department of Protein Engineering, Genentech, Inc., South San Francisco, California) Semi-random Circular 398 X2CX3CX2 phage display library 9 Sidhu SS (Department of Protein Engineering, Genentech, Inc., South San Francisco, California) Semi-random Circular 399 X2CX8CX2 phage display library 14 Sidhu SS (Department of Protein Engineering, Genentech, Inc., South San Francisco, California) Semi-random Circular 400 X2CX10CX2 phage display library 16 Sidhu SS (Department of Protein Engineering, Genentech, Inc., South San Francisco, California) Semi-random Circular