376 CSNRDARRC CANKDVRRC CANLDTRRC CPNQDSRRC CPNGDERNC CVNNDGRLC C-x-N-x-D-x-R(2)-C 6 Phage display (subtractive panning) 2-4 PMID:17259343 HT-1376 human bladder tumor cell line Not determined. Not determined. Not determined. CX7C T7 phage display library The tumor cells were isolated from tumor xenografts in nude mice prepared using the HT-1376 human bladder tumor cell line. Phage that bound to normal bladder cells isolated from mouse bladder tissues, normal rat kidney cells, or human umbilical vascular endothelial cells were subtracted during the biopanning procedure. After the second round of screening, the numbers of phage were enriched approximately by 1,000-fold and did not increase in further steps. Ninety-six phage clones were picked from the enriched phage library after the second and third rounds of screening. Twenty of the 96 clones showed selective binding to HT-1376 cells. These clones were sequenced. Six sequences were given in the published paper. It has been shown that CSNRDARRC selectively targeted and bound to primary bladder tumor tissues and can be used to detect tumor cells in urine. 377 AFNMFDFGV VTKSLRVFG SESHRVKSA QLGPPV 4 Phage display (common panning) 1-2 PMID:17129616 Anti-HAV polyclonal antibody IgG Hepatitis A virus, HAV Not determined. Not determined. J404 phage display library (X9) Mouse anti-human IgG coupled magnetic microbeads were blocked overnight at 4 ◦C in PBS/0.1% BSA (w/v). These beads were then incubated overnight at 4 ◦C with anti-HAV serum and washed four times with PBS/0.1% BSA (w/v)/0.1% Tween 20 (v/v) (PBSB-T). The beads were further blocked with excess of UV-killed wild-type phage M13K07 particles for 4 h at 4 ◦C and then used to screen the library. Since there was no enrichment after a second round of affinity selection, the 75 selected clones from the first round of panning, were tested. After a series of immunoscreenings, 4 clones are found to be disease-specific. Among them, VTKSLRVFG was recognised by 92% of the positive sera. Three of the four cloned peptides showed similarity in their amino acid sequences with at least one of the VP3 and VP1 antigenic proteins of HAV. 378 SVSVGMKPSPRP(25) WPLHTSVYPPSP(11) NTLPPFSPPSPP(5) SFPDSNIAPSSP(3) QHAPSNSKSVLT(2) WPTYLNPSSLKA(1) GPSGNLHIRPAS(1) SPLLSTRAVQLS(1) SPMFTMIQGDAQ(1) VNSHQALWSPAQ(1) STLPPPLRFANV(1) SFNQPYLYKTAF(1) YHTRIALPDNLP(1) AQSTAFQKPLLM(1) 14 Phage display (in vivo) 5 PMID:18006841 Non-small cell lung cancer (CL1-5) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) CL1-5 cells (lung cancer cell line) were injected s.c. into SCID mice to produce lung cancer xenografts. The paper suggested that PSP motif was crucial for peptide binding to the tumor neovasculature. The most frequent peptide SVSVGMKPSPRP was shown to be a good candidate for targeted drug delivery to solid tumors. 379 PPWDFDAGEGIH(1) DYAWDDFYAMGD(1) 2 Phage display (common panning) 3 PMID:17371999 Seam 3 monoclonal antibody Neisseria meningitidis serogroup B capsular polysaccharide (MenB CP) Not determined. Not determined. X9 phage display library 380 DYAWDQTHQ(3) DAGEGGPRV(2) DAGDSGYLT(1) DAGDHSHPQ(1) DAGEVYPGP(1) DAGDSAYSQ(1) DAGDHRAAA(1) EFDAGDVLL(1) D-A-G-[ED] 8 Phage display (common panning) 3 PMID:17371999 Seam 3 monoclonal antibody Neisseria meningitidis serogroup B capsular polysaccharide (MenB CP) Not determined. Not determined. X12 phage display library DYAWDQTHQ has been shown to be a promising lead candidate for the development of an effective and affordable anti-MenB vaccine. 381 ISYEHPLLTGLLLEQRHVD LDGDVSKLHPLLVNFLKGD VESSHPLLVGLLGKQQATV SEGLHPLLTALLAPQVTQW ALHDQQSLHPLLAEALLRD PMVQHPWLVGLLVSAEGVS DLAEPVKLHPLLSMYLSNL VAQMHPGLSELLLASHFHQ VHVTMPFLTSLLTGQPTSV MPLHSPYLLSLLTGSGQSL VDLEYPMLVHLLQYGRELS PVETHPRLLKLLTRAEYPM TEVTHPLLASLLQYGVQVS ATTPPTQLHPLLTQFLRTD SSLEHPWLTMLLGKPHNMW PVTAYPMLAELLLPTGQLR MSQSVSDLHPLLGLLLTAD VATNHPWLTMLLNGQASGE PLALHPLLASLLDPGRQLV TQMEYPLLTQLLGGRTMEE LLEAHPLLTGLLGASSLEL ILEEHPLLASLLTETSRGM LSQEYPWLTRLLDSGQLGS TWLEYPLLTDLLLPSWRQD SAADHPSLLRLLTGDFPYE ISYEHPLLTGLLLDSVMW LELEATSLHPLLLQLIKQV PVFEHPRLVGLLVGTDIAS LGESHPLLMQLLTENVGTH VEENPLLLEMLLSASKASS MVAQNPLLGGLLLGSMDTM PTSSPLLLDLLLGRPVETV PFYTHPMLSSLLLGPPSMT PTVEHPMLSSLLRGAANEF TVVQHPWLTGLLIGSGQDA SLEAHPLLSALLQDQMGGD PLSDHPMLLELLNHNSMRN HQEHPLLLHMLLGGSQEVE NHETHPLLSKLLLSPLEQT MSDHPLLLSYLLETTAVLP GVLDHPLLFQLLSSETRVL IAGEHPMLWGLLRAGGLPE PADNMPLLRTLLLGSGIEV TIDSHPMLHSLLSQRGLVA VAHTHPLLVGLLKGSITGP ALSDSPLLLSLLLGHPDWQ TYVDHPLLAELLLGEVGMN ADGSPMELHPLLSRLITAP PVEGHPWLLGLLVGAEVDV ALSSPSDLNPLLTSLLSQS MTLEHPGLTALLASQSLMG SVDQHPMLMDLLTGLKRAE MVEHPRLLSLLLDRSDVPM H-P-L(2)-x(2)-L(2) 53 Phage display (common panning) 3 PMID:17595321 Peroxisome proliferator-activated receptor gamma, PPAR-gamma PPAR-gamma coactivators Not determined. Not determined. X7-LXXLL-X7 M13 phage display library PPAR-gamma was expressed in Sf9 insect cells and biotinylated. 382 SLLHPLLVDLLQMGGAS SSVLNPLLAELLASGSS SYLLDLLLGTSDVTTNV L-x(2)-L(2) 3 Phage display (common panning) 3 PMID:17595321 Peroxisome proliferator-activated receptor gamma, PPAR-gamma PPAR-gamma coactivators Not determined. Not determined. SS-X16-S M13 phage display library PPAR-gamma was expressed in Sf9 insect cells and biotinylated. Of the 130 peptides sequenced, 53 exhibited unique sequences. 383 AVGLSPDGSRGV(10) GGSGDSRPPILG(1) TDTRPAHTGDAQ(1) PHIQADTRPHA(1) PDRPVPSLPITW(1) TPDSRGIHGAPS(1) PDGRPSPGHLPA(1) LLADTTHHRPWT(1) P-D-[TSG]-R-P 8 Phage display (common panning) 4-5 PMID:17725384 Anti-MUC1 monoclonal antibody PR81 Mucin-1, MUC-1 Not determined. Not determined. Ph.D.-12 phage display library (X12) The library was first screened against an irrelevant monoclonal anti-digoxin antibody that would not be expected to bind specifically to MUC1-specific epitopes. Additionally, in order to omit those phages that bind to BSA, the library was screened against BSA-coated wells. Fourty clones were randomly selected from each of the fourth and fifth rounds of biopanning. Among the 80 clones, 17 phage clones that showed higher binding activities (7 clones from the fourth round and 10 clones from the fifth round) were sequenced. 384 GHLSDWVYVPMR(5) VSMDDGWVFVQP(4) SHKSDWIFLPNA(3) AHKSDNWVFLPE(1) THINEKWVFLPQ(1) 5 Phage display (common panning) 4 PMID:17916189 Gamma-aminobutyric acid receptor-associated protein Calreticulin 3DOW, Not determined. Ph.D.-12 phage display library (X12) In fact, recombinant GST-GABARAP fusion protein was used for screening. Seventy randomly chosen true positive clones were sequenced. Only those with high occurrence and/or high affinity to GABARAP were given in the original paper. Calreticulin and GABARAP interact with a dissociation constant Kd=64 nm and a mean lifetime of the complex of 20 min. Thus, the interaction between GABARAP and calreticulin is the strongest so far reported for each protein. 385 KSLSRHDIHHHH(4) 1 Phage display (common panning) 4 PMID:17214965 Anti-HGF-cMET monoclonal antibody SFN68 Hepatocyte growth factor-hepatocyte growth factor receptor complex (HGF-cMET) Not determined. Not determined. Ph.D.-12 phage display library (X12) Ph.D.-7, C7C and 12 libraries were used in panning. Of 14 clones with specific reactivity, four shared the amino acid sequence KSLSRHDHIHHH. This is the only mimotope given in the original paper. 386 RVPPRYHAKISPMVN(20) 1 Phage display (common panning) 4 PMID:17005302 Anti-GMDP monoclonal antibody E6/1.2 N-acetylglucosaminyl-beta1-4-N-acetylmuramyl-alanyl-d-isoglutamine (GMDP) Not determined. Not determined. f3-15mer phage display library (X15) Twenty clones were selected and sequenced. All of them has the identical sequence RVPPRYHAKISPMVN. 387 YQDSAKT(17) NDRGLLA(5) VSVGMLW(4) QDTGLLA(2) SNPGWLI(2) 5 Phage display (common panning) 4 PMID:17761669 Beta amyloid peptide (12-28) Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Only peptides found in two or more clones are shown. 388 SVLDRQR(15) SARTFLP(3) VDANKFL(2) SHREPLQ(2) 4 Phage display (common panning) 4 PMID:17761669 Beta amyloid peptide (25-35) Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Only peptides found in two or more clones are shown. SVLDRQR was found to be effective blocker of the toxic effect of β-amyloid on cultured neuronal cells. 389 LGSYKPS(7) IQLHPRL(4) YGAAKSG(3) QATGLLA(3) SFHPPSM(2) QDTGLLA(1) EWGGAQF(1) 7 Phage display (common panning) 4 PMID:17761669 Beta-amyloid protein 42 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) LGSYKPS was found to be effective blocker of the toxic effect of β-amyloid on cultured neuronal cells. 390 NIPTPKP(3) SARTFLP(2) QATGLLA(2) IQSPHFF(2) SMPTYNK(2) GTHVQAT(2) VSVGMLW(2) NDRGLLA(1) 8 Phage display (common panning) 4 PMID:17761669 Beta amyloid peptide (1-20) Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) VSVGMLW was found to be effective blocker of the toxic effect of β-amyloid on cultured neuronal cells. 391 CPYANHLLC(11) CPYANPSMC(4) CPYHNQFLC(2) CPKSAHKHC(2) CLYTNPALC(1) P-Y-x-N-x(2)-L 5 Phage display (subtractive panning) 3 PMID:18025245 Anti-PYANPSL polyclonal antibody IgG Peptide PYANPSL Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 392 CWAANPSMC(6) CRALNPSMC(5) CPINPSMAC(4) CPFNPSMAC(3) CLNPSSSQC(2) CPYNPSAHC(1) CYNPSSSWC(1) NPSM 7 Phage display (subtractive panning) 3 PMID:18025245 Anti-WAANPSM polyclonal antibody IgG Peptide WAANPSM Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 393 NPGTCKDKWIECLLNG(9) LKNYCRKCSNRCTPTG(4) NLIWCRKEFARCTSDM(3) 3 Phage display (subtractive panning) 3 PMID:17803452 Tumor-associated glycoprotein (TAG-72) Not determined. Not determined. Not determined. f88-Cys6 phage display library (X4CX6CX4) Twenty-three clones were selected for sequencing. However, only above squences were given in the original paper. NPGTCKDKWIECLLNG showed evidence of significant specific binding when presented as the radiolabeled phage to tumor in vivo and especially in vitro with cells and solid tumor. 394 EDIKPKTSLAFR(6) TQPADLQTHNHN(1) FDHSSKWTRTSP(1) YSHNTITNLYFS(1) WPRYAESTLQLR(1) 5 Phage display (in vivo) 3 PMID:17687498 Nasopharyngeal carcinoma cell CEN-1 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) After the fifth round, all sequenced clones have the same sequence EDIKPKTSLAFR, which might be a candidate as diagnostics and therapeutics for nasopharyngeal carcinoma. 395 SVSVGMKPSPRP(2) IHPPTPPLTMLR(1) HLWPVNVASAQS(1) GSSRAQMTSHRP(1) SVGSSPHPGRHT(1) SDSPHGAVPPLA(1) HWTPWQHVSSFW(1) HPPDNARRIGCR(1) WSKNNKSPQLSP(1) GSMCPYVRWYTP(1) LNAAYSATFMAR(1) S-V-S-x(2)-M-x-P-S-P-R-P 11 Phage display (common panning) 6 PMID:18053412 l,2-Dioleoyl-sn-glycero-3-phosphatidylserine Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Ligands that bind to PS can be used for noninvasive imaging of therapy-induced cell death, particularly apoptosis. 396 CFHNHNPLC(20) CTVNTQSKC(10) CNHMPHPLC(5) CVGQKQESC(5) CLKAFHKEC(3) CHNQPQATC(3) CGPTPTKTC(2) CPLFSSKTC(2) CLTKSYTSC(2) CVTQHRGDC(2) CAPNSHWTC(1) CRLDHALQC(1) CILNNTPWC(1) CKDANSPHC(1) CPTRAALTC(1) CQKHNTRFC(1) CMPHTHRIC(1) CSQSHPRHC(1) CTQPRSTGC(1) CFPNTRATC(1) CSPVHQQSC(1) CPKSDHKFC(1) CAPKQTQHC(1) 23 Phage display (subtractive panning) 1 PMID:17324139 Mouse inflammatory small bowel Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) SQSHPRH has a specific high affinity for inflammatory bowel. 397 HPAIVHISPQWA(1) ISPAPHLLTSRF(1) HHVDSLPTLDWK(1) MPTSSTAPPPLI(1) QMVYGPLRSTEQ(1) HGTNQALSLLTP(1) KLPHQPPSAAVH(1) ANYFSSPIKHAT(1) NSLTSEPLRYGG(1) VLNPQTTVMPPL(1) SPWTSFLQWARG(1) HPPHNMHLPAFS(1) APFAHSGPLAFS(1) ATTSLTPTMANH(1) QFPPKLTNNSML(1) MPTLTRAPHTAC(1) TPLHPKSLMVWH(1) QATGPTTPTTSG(1) YTDNSLGTSVGK(1) LPRKTPDYLQTR(1) SNSMHLMTMTGL(1) TPSLEQRTVYAK(1) TSLRELPAEWSR(1) SVPPRYTLTLQW(1) LYSASTPPDPGG(1) SHGKPPSRSPWT(1) TLDWTKPPLRSG(1) FPMSSYKTYATP(1) YNLGQLEAQITS(1) ASQGYPEHRHAS(1) INTPANRNPVLG(1) ITLSATKGAAPS(1) HKSYLPVPSLYG(1) WDTNRNAASTPG(1) SPPPSNAGSHHV(1) QTTKLHIMDTGF(1) SYQTLKQHLPYG(1) THPPNPSVSIGG(1) S-P-T-P-S-[PL]-P(2)-S-A-G(2) 38 Phage display (subtractive panning) 3 PMID:17657770 Human blood outgrowth endothelial cells, HBOEC Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Before HBOEC screening, human umbilical vein endothelial cells (HUVEC) were used to remove non-specific binding phages. Fourty clones were sequenced. 38 different peptide sequences were deduced and 2 phage clones contained no inserts. 398 AMSFYNTPDAKE(1) TLCNNSMSPGCK(1) IEHTRYNSRTAY(1) QATKYHSANRHP(1) NYLHNHPYGTVG(1) GQSPHSYQPRTY(1) NNALLNRQLHFS(1) HQVPSHNNSLGP(1) 8 Phage display (common panning) 3 PMID:16958043 Beta-nerve growth factor, Beta-NGF Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) These peptide sequences were obtained when the pH of elution buffer was at 6.0. 399 RSTSPDPLNYVR(1) ALRTPLSHAPTK(1) NMTAVTKLPAPW(1) QMHPSIALPLWY(1) QMHPSIALPHWY(1) LDLALGQRPPRH(1) DARHLPPVTLST(1) SVSVGMKPSPRP(1) 8 Phage display (common panning) 3 PMID:16958043 Beta-nerve growth factor, Beta-NGF Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) These peptide sequences were obtained when the pH of elution buffer was at 4.5. 400 SVSVGMKPSPRP(2) DSAVDYTGWLRR(1) SVWYYXIHYDMX(1) SLTGAALGPSGW(1) VPHSKYPRGINA(1) QFSLPVAKLVNR(1) 6 Phage display (common panning) 3 PMID:16958043 Beta-nerve growth factor, Beta-NGF Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) These peptide sequences were obtained when the pH of elution buffer was at 2.8.