3676 GLHTSATNLYLH(2) SLDGAGAALRTS(2) DRWVARDPASIF(1) GDGNSVLKPGNW(1) HTPMSSRLSTAS(1) SGVYKVAYDWQH(1) SKGDSLPFPFAT(1) SNSIDKVNRPIN(1) VVSPDMNLLLTN(1) 9 Phage display (subtractive panning) 4 PMID:36746976 Vitamin D binding protein, VDBP Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Data are expressed as the means of the absorbance value at (410 ± SD) nm. One point five mL of phage pool diluted with TBST (TBS + 0.1%.v/v Tween-20) to 1.0e9 Plaque Forming Units per mL (pfu/mL) from the phage library was attached to the petri dish (polystyrene). Then, the solution was rocked at room temperature (RT) for 45 min to remove phages attached to the petri dish (polystyrene) first. The BSA-coated plate was washed 5 times with TBST. Then, the supernatant of the phage pool reacted with a petri dish (polystyrene) and was recovered and put into the BSA-coated plate. Also, it was bound by rocking at room temperature (RT) for 45 min. After that, unbound phages were collected and used for main biopanning. 3677 YEFHPMGNPLHR(21) SYPSNALSLHKY(1) GTGGVHPATKLT(1) HDPRMEHSLPKS(1) ISAKPIPISMRN(1) SYPSNALSLHKY(1) TASSINLHAAHE(1) TLNVPPAKRSLS(1) TSLSTAHPMLYQ(1) 9 Phage display (subtractive panning) 6 PMID:36746976 Petri dish (polystyrene, PS) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Data are expressed as the means of the absorbance value at (410 ± SD) nm. After positive biopanning against the target, all 1.15 mL of neutralized phages were treated in a petri dish (polystyrene), and the solution was rocked at room temperature (RT) for 30 min. Only the supernatant from which the phage strongly bound to polystyrene was removed was collected. 3678 YEFHPMGNPLHR(21) HDPRMEHSLPKS(5) NTTYPTVYADKS(1) SYWYEASSYTGV(1) TNENLMVRLTHA(1) 5 Phage display (subtractive panning) 7 PMID:36746976 Petri dish (polystyrene, PS) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Data are expressed as the means of the absorbance value at (410 ± SD) nm. After positive biopanning against the target, all 1.15 mL of neutralized phages were treated in a petri dish (polystyrene), and the solution was rocked at room temperature (RT) for 30 min. Only the supernatant from which the phage strongly bound to polystyrene was removed was collected. 3679 SLFTKQYDYFDT(5)[0.6461±0.0288] SFTKTSTFTWRD(2)[0.7244±0.0426] AMPPTDLFLHSK(2)[0.3140±0.0110] ANGTAHSTPLLW(2)[0.2769±0.0124] DRPAHGILEASL(2)[NT] FSPQNHKPNPVT(1)[0.3071±0.0193] IPQRYAPVSNLP(1)[NT] NNSHYYRNIFYT(1)[NT] TSTLYTRAQLWN(1)[NT] 9 Phage display (subtractive panning) 6 PMID:36746976 Vitamin D binding protein (VDBP) complex, VDBP-Complex Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Data are expressed as the means of the absorbance value at (410 ± SD) nm. After positive biopanning against the target, all 1.15 mL of neutralized phages were treated in a petri dish (polystyrene), and the solution was rocked at room temperature (RT) for 30 min. Only the supernatant from which the phage strongly bound to polystyrene was removed was collected. 3680 SLFTKQYDYFDT(6)[0.6461±0.0288] VPTTSHRVAVLS(3)[0.3468±0.0331] FSPQNHKPNPVT(2)[0.3071±0.0193] AMPPTDLELHSK(1)[0.3140±0.0110] IPQRYAPVSNLP(1)[NT] NNSHYYRNIFYT(1)[NT] SSAPSMVMSTLF(1)[NT] SWNHAGQPLTVV(1)[NT] SYPSNALSLHKY(1)[0.3523±0.0207] 9 Phage display (subtractive panning) 7 PMID:36746976 Vitamin D binding protein (VDBP) complex, VDBP-Complex Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Data are expressed as the means of the absorbance value at (410 ± SD) nm. After positive biopanning against the target, all 1.15 mL of neutralized phages were treated in a petri dish (polystyrene), and the solution was rocked at room temperature (RT) for 30 min. Only the supernatant from which the phage strongly bound to polystyrene was removed was collected. 3681 TGSAKFLQRDTH(3)[0.3523±0.0097] AFADGYSARRNL(1)[NT] AFKPTSGLAKLS(1)[NT] AWRPFPSATSGP(1)[NT] FAPYNNLSDNYP(1)[NT] YHGQISANAHGW(1)[NT] YSSIAPSISNAL(1)[NT] YSSTSYRALTLG(1)[NT] YTSLPTEATDRT(1)[NT] 9 Phage display (subtractive panning) 6 PMID:36746976 Vitamin D binding protein, VDBP Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Data are expressed as the means of the absorbance value at (410 ± SD) nm. After positive biopanning against the target, all 1.15 mL of neutralized phages were treated in a petri dish (polystyrene), and the solution was rocked at room temperature (RT) for 30 min. Only the supernatant from which the phage strongly bound to polystyrene was removed was collected. 3682 TGSAKFLQRDTH(3)[0.3523±0.0097] ATWWQPDARGTP(1)[NT] ATYQNWTLPHRV(1)[NT] AWRPSSASTLWN(1)[NT] GSAARTISPSLL(1)[0.4401±0.0715] SPAKPHSFYTGS(1)[NT] SVPLNSWSIFPR(1)[NT] TADVFSSSRYTR(1)[NT] VQFTPRSYQPIY(1)[NT] 9 Phage display (subtractive panning) 7 PMID:36746976 Vitamin D binding protein, VDBP Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Data are expressed as the means of the absorbance value at (410 ± SD) nm. After positive biopanning against the target, all 1.15 mL of neutralized phages were treated in a petri dish (polystyrene), and the solution was rocked at room temperature (RT) for 30 min. Only the supernatant from which the phage strongly bound to polystyrene was removed was collected.