3151 VSRDTPQ(28.4%) SYTPGGH(16.7%) LIQPITT(7.4%) QLYREFN(4.2%) MDTPDRI(3.5%) WVTDSSW(3.2%) HSPNSIK(2.9%) RFDWSYP(2.9%) AYASDSY(2.7%) YNISVNK(2.6%) 10 Phage display (competitive panning) 1 PMID:26246327 Sera of peanut-tolerant subject 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3152 VSRDTPQ(79.0%) SAEKLLR(6.9%) YNISVNK(3.0%) QLYREFN(2.4%) WVTDSSW(1.7%) HSPNSIK(1.4%) GQSEKHL(1.3%) RFDWSYP(1.2%) LPGNRLL(0.9%) RTWEPYT(0.5%) 10 Phage display (competitive panning) 2 PMID:26246327 Sera of peanut-tolerant subject 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3153 VSRDTPQ(80.3%) SAEKLLR(13.2%) GQSEKHL(3.5%) YNISVNK(1.1%) WVTDSSW(0.8%) QLYREFN(0.4%) LPGNRLL(0.3%) RFDWSYP(0.3%) HSPNSIK(0.0%) RTWEPYT(0.0%) 10 Phage display (competitive panning) 3 PMID:26246327 Sera of peanut-tolerant subject 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3154 SNYHPSI(51.2%) TTQVLEA(3.1%) KMISATE(2.9%) LPQSWAM(1.8%) ATAEWHP(1.7%) APANTFT(1.7%) VPPTEHP(1.3%) LGQLFPQ(1.3%) MIHPRDQ(1.1%) FTQPLYK(1.1%) 10 Phage display (competitive panning) 1 PMID:26246327 Sera of peanut-tolerant subject 3 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3155 SNYHPSI(61.0%) KMISATE(6.2%) AERYPDS(5.2%) KMESGTA(3.7%) AKYEAGS(3.2%) TTQVLEA(1.9%) TEQKIEA(1.9%) TITAMQG(1.4%) AERILSV(1.3%) GWETRME(1.2%) 10 Phage display (competitive panning) 2 PMID:26246327 Sera of peanut-tolerant subject 3 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3156 AERYPDS(56.8%) SNYHPSI(27.6%) KMISATE(8.0%) ELGTTQT(2.3%) TTQVLEA(1.0%) AKYEAGS(0.7%) GWETRME(0.5%) NDRPHMP(0.3%) ADLMHFT(0.3%) VPPTEHP(0.3%) 10 Phage display (competitive panning) 3 PMID:26246327 Sera of peanut-tolerant subject 3 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3157 KSLNSTI(17.2%) VPILNSL(7.1%) SMNETFA(6.6%) WSLSELH(3.7%) NTDTTRE(3.3%) VNQSWDL(2.0%) RDEVYFH(1.9%) NEAPRHA(1.7%) IDRTQFM(1.5%) NTVGTVQ(1.3%) 10 Phage display (competitive panning) 1 PMID:26246327 Sera of peanut-tolerant subject 4 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3158 VPILNSL(19.8%) WSLSELH(17.0%) ENHVHVR(13.2%) QETRGTY(4.8%) IDNSHTH(4.0%) TTANVRI(3.9%) VNQSWDL(3.1%) HAAWHFI(2.9%) EPAIATP(2.3%) NEAPRHA(2.3%) 10 Phage display (competitive panning) 2 PMID:26246327 Sera of peanut-tolerant subject 4 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3159 WSLSELH(20.6%) HAAWHFI(19.3%) ENHVHVR(16.7%) VPILNSL(7.6%) VTLPPSS(3.8%) TTANVRI(3.4%) ESKWMPL(3.2%) IDNSHTH(3.2%) QETRGTY(3.0%) GTMHSMK(2.6%) 10 Phage display (competitive panning) 3 PMID:26246327 Sera of peanut-tolerant subject 4 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3160 MGQPTVK(58.0%) GPLKTWK(18.6%) GTLASLN(5.4%) KVKTYDM(3.4%) QQLAVDT(2.7%) STLNWWN(1.4%) QTNMRAP(0.4%) ESRVMSR(0.3%) EARMHRA(0.3%) TYPSLWK(0.3%) 10 Phage display (competitive panning) 1 PMID:26246327 Plasma of peanut-tolerant subject 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3161 QQLAVDT(39.8%) MGQPTVK(16.2%) TTQVLEA(8.8%) WEGPQPK(6.9%) GPLKTWK(6.8%) ESRVMSR(4.1%) GTSDKLP(2.3%) KDNTYVM(2.3%) STLNWWN(2.1%) GTLASLN(1.3%) 10 Phage display (competitive panning) 2 PMID:26246327 Plasma of peanut-tolerant subject 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3162 QQLAVDT(64.5%) ESRVMSR(14.4%) WEGPQPK(7.9%) ALGTRMI(6.5%) STLNWWN(2.7%) KDNTYVM(1.0%) TTQVLEA(0.8%) GTSDKLP(0.5%) HSHTLTW(0.4%) YPWFIRA(0.3%) 10 Phage display (competitive panning) 3 PMID:26246327 Plasma of peanut-tolerant subject 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3163 DVHKLGN(23.8%) ISHTKAE(10.0%) NDIPHLM(9.1%) RIHVHNS(4.4%) AQSLETS(3.5%) SLTMKAN(2.1%) DLNMPIS(2.0%) LYADSVL(1.9%) ITPAYDN(1.8%) SVASTTV(1.7%) 10 Phage display (competitive panning) 1 PMID:26246327 Plasma of peanut-tolerant subject 3 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3164 DVHKLGN(37.4%) AQSLETS(12.9%) NDIPHLM(12.3%) ETALIAA(7.5%) QQLAVDT(3.8%) RIHVHNS(3.5%) LYADSVL(3.2%) SVASTTV(3.2%) DLNMPIS(2.1%) IAGPFKL(1.6%) 10 Phage display (competitive panning) 2 PMID:26246327 Plasma of peanut-tolerant subject 3 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3165 AQSLETS(40.5%) ETALIAA(16.6%) QQLAVDT(11.0%) DVHKLGN(10.8%) LYADSVL(7.7%) RIHVHNS(3.1%) NDIPHLM(2.8%) NPMYHSS(1.9%) KQEAMTL(1.1%) QNSPFSE(1.0%) 10 Phage display (competitive panning) 3 PMID:26246327 Plasma of peanut-tolerant subject 3 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3166 RIRSEEL(24) TVVVAAG(17) VQLSIIV(15) PRTTIMG(15) KFVVAFC(14) LAPREGA(13) LMTARWC(13) FWALATW(13) YCVGDGC(13) WVNAATC(12) SRGVGVG(12) RRRPGAV(12) QLVRGRW(12) SVFIMLR(11) VFMGGCR(11) TSEGALR(11) SSRSSGG(11) GRLSADG(11) RRAVRFM(11) GLCAEAC(10) 20 0 PMID:22870226 Not determined. Not determined. Not determined. Not determined. pVIII fth1-dp-based X7 phage display library (X7) For the analysis of the naive libraries, four samples of 4.0e8 phages each were amplified individually by the PCR reactions. The amplified PCR products were validated for size (152 bp) by running in 2% agarose gel. All 4 PCR cleaned products were united and their concentration was measured. The sample was dried by speed vac and sent for Illumina sequencing. The 20 top most frequent peptides for each capture are given. 3167 ADGIVGW(3105) LAAGAVW(3039) SAGFAME(2944) VSLCSSR(2925) SVTDYVE(2855) RMGIRAL(2659) DDDGLDG(1931) SAARVFM(1723) QLYGARE(1716) NRSREMG(1707) IVPACRG(1646) RLVYVPS(1335) TGCSSIL(1166) AFHSGLT(1106) CSWTLER(996) GGGVGLL(912) WHAQVGF(852) ERRMGSC(844) SGVGATH(770) RLRWWGR(738) 20 Phage display (common panning) 1 PMID:22870226 Anti-gp120 monoclonal antibody GV4H3 Surface protein gp120, SU Not determined. Not determined. pVIII fth1-dp-based X7 phage display library (X7) the captured phages were eluted and directly amplified by PCR. The 20 top most frequent peptides for each capture are given. 3168 ADGIVGW(11489) SAGFAME(5173) LAAGAVW(3140) VTPHTGF(1172) GAHVAGG(672) LTACTGF(660) LGWAVLD(506) GTASVGF(444) SLGWAVP(355) GVGWAVP(344) AVGWAVP(336) GPGMALE(324) PASLVGF(300) SAGWALP(293) EVGWAVH(272) LNSMVGF(226) VGWAVLE(222) QMPNLGF(215) LVGWALS(202) SAGWALE(201) 20 Phage display (common panning) 2 PMID:22870226 Anti-gp120 monoclonal antibody GV4H3 Surface protein gp120, SU Not determined. Not determined. pVIII fth1-dp-based X7 phage display library (X7) the captured phages were eluted and directly amplified by PCR. The 20 top most frequent peptides for each capture are given. 3169 ADGIVGW(65003) SAGFAME(6630) VGWAVLE(2409) VTPHTGF(2366) LAAGAVW(2364) SAGVAME(2242) ADGIVGG(1009) EVGWAVH(905) WVGWAVQ(834) LGWAVLD(768) AVGWAVP(536) AGWAVLE(469) LVGWALS(453) SLGWAVP(445) GAHVAGG(413) LTACTGF(368) GVGFALD(362) GVGFALE(349) RVSAEVW(346) LAGWALD(337) 20 Phage display (common panning) 3 PMID:22870226 Anti-gp120 monoclonal antibody GV4H3 Surface protein gp120, SU Not determined. Not determined. pVIII fth1-dp-based X7 phage display library (X7) the captured phages were eluted and directly amplified by PCR. The 20 top most frequent peptides for each capture are given. 3170 ASSSHRS(73,075) DLKIPLR(37,802) YRAPWPP(34,250) AMSSRSL(15,301) LPLTPLP(13,006) IEFSPLM(4,765) GETRAPL(1,279) RHEPPLA(6) 8 Phage display (common panning) 3/4 PMID:22178910 Progenitor stem cell line KS483 at 4 days of differentiation Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) KS483 cells were incubated for 10 min on ice with 3 ml of 0.1 M HCl (pH 2.2) to elute cell surface-bound phage. 3171 GETRAPL(397,669) AMSSRSL(54,629) NPWTTRP(50,155) LPLTPLP(29,175) YRAPWPP(23,765) ASSSHRS(5,033) ALPQIVR(210) 7 Phage display (common panning) 3 PMID:22178910 Progenitor stem cell line KS483 at 4 days of differentiation Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) KS483 cells were lysed for 1 h on ice in 3 ml of 30 mM Tris–HCl and 1 mM EDTA (pH 8.0) to recover the cell-associated phage fraction. 3172 GETRAPL(3,599,322) ASSSHRS(57,476) YRAPWPP(56,436) DLKIPLR(55,342) IEFSPLM(43,697) LPLTPLP(20,988) AMSSRSL(11,517) 7 Phage display (common panning) 3 PMID:22178910 Progenitor stem cell line KS483 at 8 days of differentiation Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) KS483 cells were incubated for 10 min on ice with 3 ml of 0.1 M HCl (pH 2.2) to elute cell surface-bound phage. 3173 RHEPPLA(543,337) GETRAPL(427,153) AMSSRSL(134,645) YRAPWPP(55,732) ALPQIVR(34,034) ASSSHRS(24,902) WQSVPTI(20,390) LPLTPLP(20,003) DERHQHY(19,404) DLKIPLR(59) 10 Phage display (common panning) 1-4 PMID:22178910 Progenitor stem cell line KS483 at 8 days of differentiation Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) KS483 cells were lysed for 1 h on ice in 3 ml of 30 mM Tris–HCl and 1 mM EDTA (pH 8.0) to recover the cell-associated phage fraction. 3174 HAIYPRH(36) GKPMPPM(35) NHWASPR(33) SPPPPPP(32) TPPPPPP(31) VGYRLME(25) LPPPPPP(24) ATSAIHG(24) SPPVLPH(24) STYRITT(23) LSPLTPP(23) 699725 0 PMID:22178910 Not determined. Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Before analyzing the phage display libraries selected against biological targets, we screened for potential sequence biases introduced by the propagation of the phages in bacteria by sequencing the naive (unselected) library after one round of bacterial amplification. 3175 WPTDHQMLRIPM(342) DWSSWVYRDPQT(143) QSDPWLVSRWFA(66) TYPSTQWFFAKF(60) TLPPEAWWRTMY(52) YPSSEQLLAWWG(40) ELPSVEQLWDFF(37) WPWDPLRISDWL(36) TWVSDLDMWLGA(28) RLPTSMELLAAF(24) QDPWKVKNWINQ(15) FFPSEQVLIAAL(12) KLPSPYDLYLFL(9) WDPWQIDRWVLL(8) NAPSIYDWLATL(5) WPLWSFDWPQNA(4) WLTEDMSAFMSY(4) DPWFINEWIRIP(4) WPSDNQLLQIHV(2) SWDPLLVSAWIK(1) 20 0 PMID:26161996 Not determined. Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The Ph.D. library was amplified once with no selection and used as the unselected library control (replicate 1).