3126 KSLSRHDHIHHH(6) QVHINHISPSVP(2) KSLSRHDHLLPH(1) QSLSRHDPILHH(1) SFQAGIRSAPEP(1) SFQVGIRSAPEP(1) 6 Phage display (common panning) 3 PMID:28272485 Anti-PRRSV monoclonal antibody A2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Anti-porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing monoclonal antibody A2 recognize the mimotope (KSLSRHDHIHHH), P2, which contains the SRHDHIH motif, which has conserved consensus sequences from amino acid positions 156 to 161 in the N-terminal ectodomain of GP3. The artificial multi-epitope gene, mp2, was designed by combining three repeats of the mimotope P2. The resulting sequence was inserted into the swinepox virus (SPV) genome to construct a recombinant swinepox virus (rSPV-mp2). The rSPV-mp2 was able to stably express the multi-epitope peptide, mP2, in vitro. The rSPV-mp2 immunized pigs exhibited a significantly shorter fever duration compared with the wtSPV treated group (P < 0.05). There was an enhanced humoral and cellular immune response, decreased number of PRRSV genomic copies, and a significant reduction in the gross lung pathology (P < 0.05) was observed following PRRSV infection in rSPV-mp2-immunized animals. 3127 KSLSRHDHIHHH(3) GPRPPMTSTIFP(2) KSFSRHDIHHH(1) KSLSRHDIHQH(1) KSLSRQDIHRH(1) KSVSRQDIHHQ(1) KSSSRHYYLHHQ(1) GPRPPTWSPGQV(1) GPRRRKWYLGQV(1) 9 Phage display (common panning) 3 PMID:28272485 Anti-PRRSV monoclonal antibody A7 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Anti-porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing monoclonal antibody A7 recognize the mimotope (KSLSRHDHIHHH), P2, which contains the SRHDHIH motif, which has conserved consensus sequences from amino acid positions 156 to 161 in the N-terminal ectodomain of GP3. The artificial multi-epitope gene, mp2, was designed by combining three repeats of the mimotope P2. The resulting sequence was inserted into the swinepox virus (SPV) genome to construct a recombinant swinepox virus (rSPV-mp2). The rSPV-mp2 was able to stably express the multi-epitope peptide, mP2, in vitro. The rSPV-mp2 immunized pigs exhibited a significantly shorter fever duration compared with the wtSPV treated group (P < 0.05). There was an enhanced humoral and cellular immune response, decreased number of PRRSV genomic copies, and a significant reduction in the gross lung pathology (P < 0.05) was observed following PRRSV infection in rSPV-mp2-immunized animals. 3128 YAAHRSH(5/50) KFPAINQ(5/50) GVQSPHF(4/50) VPFPSAS(2/50) GIHTLMG(2/50) ATWVSPY(2/50) GSTPNWH(2/50) IDPQTPT(2/50) ADRAWAR(2/50) NHRPHLD(1/50) TNPHLNW(1/50) AFPSPTD(1/50) STSPESA(1/50) ALHGPTP(1/50) APSFTKH(1/50) DFHHASM(1/50) FPPFQTQ(1/50) GHYSAGC(1/50) HSAHENS(1/50) ISPQTPT(1/50) LIAASNN(1/50) LPPSSRM(1/50) NSHSMPF(1/50) QFATQSH(1/50) QIGYQRA(1/50) QSFWFHA(1/50) SVSPISH(1/50) TPLTPHQ(1/50) TSSHAPL(1/50) VALSAPY(1/50) VAPWEKL(1/50) VIIVPPA(1/50) YIPTAMK(1/50) 33 Phage display (common panning) 3 PMID:28252649 Metacaspase, MCA Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Four peptides, NHRPHLD, YAAHRSH, ATWVSPY and AFPSPTD, were selected for their capacity to specifically bind to MCA and interfere with its activity. The peptide with the sequence of YAAHRSH, similar to ecotin-like ISP3 of L. (L.) major, decreases trypsin-like activity of promastigotes under heat shock, and significantly decreases parasite heat shock-induced death. 3129 YEQHKLPSSWPF(12) YQTKLQHVWPFS(4) SPYQTRLPVVWP(3) LSQSKLASPWPL(1) QISQLRLPNNWP(1) FQTRLLHTWPSP(1) EHQVRLSSQWPL(1) TEQMKLLYHWPT(1) QYKIPPLWPMLK(1) YQMKSPTPVWPF(1) HHWFKSLTTKHL(1) YQMKLPLILPPT(1) DFQNKLSSVTLR(1) ISQQPLAPVWPP(1) DAQQLYLSNWRS(1) NQHPPMAHLSAQ(1) YVHHSQLPDASL(1) HAKSQLNPPDIK(1) SAHQVPNAVIKS(1) GQGVPDLHPPLL(1) LPNPQQYTTPSS(1) RYPLESPYWQQT(1) TSPLNIHNGQKL(1) DSTNMETQTLPL(1) DLNTNRTQMVLH(1) HESFWYLPHQSY(1) 26 Phage display (common panning) 5 PMID:18959752 Protein-glutamine gamma-glutamyltransferase K Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The peptides selected as glutamine donor substrate exhibited a marked tendency in primary structure, conforming to the sequence: QxK⁄RψxxxWP (where x and ψ represent non-conserved and hydrophobic amino acids, respectively). Using glutathione S-transferase (GST) fusion proteins of the selected peptides, we identified several sequences as preferred substrates and confirmed that they were isozyme-specific. We generated GST-fused alanine mutants of the most reactive sequence (K5, YEQHKLPSSWPF) to determine the residues that were critical for reactivity. Even in peptide form, K5 appeared to have high and specific reactivity as substrate. In situ analysis of mouse skin sections using fluorescence-conjugated K5 peptide resulted in detection of TGase 1 activity with high sensitivity, but no signal was detected in a TGase 1-null mouse. 3130 WSLSELH(14.3%) DGRYYIN(9.1%) VSRDTPQ(4.5%) MHTVAVQ(4.2%) HLNQQNH(3.5%) APRNVPP(2.4%) WPFHGDN(1.5%) QISAASQ(1.5%) LPYDHLP(1.5%) YPWFIRA(1.4%) 10 Phage display (competitive panning) 1 PMID:26246327 Sera of peanut allergic patient 1 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3131 VSRDTPQ(33.9%) DGRYYIN(20.2%) WSLSELH(15.8%) HWRLPLH(7.4%) HLNQQNH(4.6%) IWRLPTH(4.2%) MHTVAVQ(2.4%) VMPLDDV(1.4%) SYTDLLR(1.2%) YPWFIRA(0.9%) 10 Phage display (competitive panning) 2 PMID:26246327 Sera of peanut allergic patient 1 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3132 VSRDTPQ(73.1%) DGRYYIN(9.5%) WSLSELH(8.7%) HLNQQNH(3.4%) HWRLPLH(0.9%) IWRLPTH(0.7%) SYTDLLR(0.7%) VMPLDDV(0.5%) YPWFIRA(0.5%) MHTVAVQ(0.3%) 10 Phage display (competitive panning) 3 PMID:26246327 Sera of peanut allergic patient 1 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3133 DNYDSTA(59.8%) MLHELLE(3.0%) RVASLAP(2.7%) ENHVHVR(2.6%) NYFKHEA(2.3%) DAIPTSV(1.8%) VSGLLVE(1.8%) ELRNENT(1.8%) NSPELTS(1.1%) NGHSIHT(1.0%) 10 Phage display (common panning) 1 PMID:26246327 Sera of peanut allergic patient 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3134 DSTA(74.0%) VSGLLVE(7.3%) ENHVHVR(5.3%) MLHELLE(4.3%) NSPELTS(2.1%) DAIPTSV(1.7%) SLLGQTP(1.1%) YPWFIRA(0.7%) NYFKHEA(0.5%) ISPLSVP(0.5%) 10 Phage display (competitive panning) 2 PMID:26246327 Sera of peanut allergic patient 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3135 DNYDSTA(64.1%) ENHVHVR(15.5%) VSGLLVE(12.7%) YPWFIRA(4.7%) MLHELLE(2.5%) SLLGQTP(0.1%) NSPELTS(0.1%) SPTTTYD(0.1%) TALRTIS(0.1%) ISPLSVP(0.0%) 10 Phage display (competitive panning) 3 PMID:26246327 Sera of peanut allergic patient 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3136 EVMGRLA(10.4%) STTPHSR(7.5%) MVPPSYD(3.4%) SSIGGQD(2.9%) QLYREFN(2.8%) YLGFDVH(1.8%) SQNTKYI(1.5%) QLIHWRH(1.3%) MVQHYAE(1.2%) HSILSNL(1.2%) 10 Phage display (competitive panning) 1 PMID:26246327 Sera of peanut allergic patient 3 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3137 EVMGRLA(72.1%) MVPPSYD(5.8%) QLIHWRH(4.8%) QLYREFN(3.6%) SSNQFHQ(1.5%) HSILSNL(1.5%) YLGFDVH(1.4%) AQYVAVG(0.9%) SSIGGQD(0.8%) ATDEIVP(0.8%) 10 Phage display (common panning) 2 PMID:26246327 Sera of peanut allergic patient 3 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3138 EVMGRLA(64.9%) MVPPSYD(8.7%) VKFDKYV(8.5%) AMSHSKQ(5.4%) QLIHWRH(3.1%) YLGFDVH(1.2%) WSLSELH(1.1%) HSILSNL(0.9%) LKFDTPI(0.9%) QLYREFN(0.7%) 10 Phage display (competitive panning) 3 PMID:26246327 Sera of peanut allergic patient 3 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3139 SLLGQTP(16.5%) STIVAEM(6.8%) GFTFQPS(5.3%) SAAWNKS(4.7%) TNLSHVP(3.4%) QTWLEMG(3.3%) LLADMHA(2.6%) GTGSQAS(2.0%) LVYVDMH(1.0%) ISYLSVT(0.9%) 10 Phage display (competitive panning) 1 PMID:26246327 Sera of peanut allergic patient 4 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3140 SLLGQTP(41.9%) SAAWNKS(16.0%) STIVAEM(12.9%) GTGSQAS(6.6%) GFTFQPS(5.9%) QTWLEMG(4.9%) TNLSHVP(2.3%) SIIARPG(1.8%) STPATLI(1.2%) LLADMHA(0.8%) 10 Phage display (competitive panning) 2 PMID:26246327 Sera of peanut allergic patient 4 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3141 SLLGQTP(50.0%) SAAWNKS(18.9%) STIVAEM(13.1%) GTGSQAS(6.2%) QTWLEMG(4.4%) GFTFQPS(2.5%) KGIYHQV(0.8%) STPATLI(0.7%) SLSSAWW(0.6%) TNLSHVP(0.5%) 10 Phage display (competitive panning) 3 PMID:26246327 Sera of peanut allergic patient 4 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3142 AMSPLNK(16.2%) MPTWLHH(13.8%) NVRLPYQ(9.2%) NLLDSLH(6.0%) NPTHPIY(3.5%) FASRSDT(2.7%) IENRIYR(2.3%) GVFISYN(2.2%) LSKINSP(2.1%) GNEVMTY(1.6%) 10 Phage display (competitive panning) 1 PMID:26246327 Plasma of peanut allergic patient 1 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3143 MPTWLHH(29.9%) AMSPLNK(17.5%) GASESYL(10.4%) GVFISYN(5.3%) FASRSDT(4.8%) IENRIYR(4.7%) NVRLPYQ(3.5%) IPNGHFT(3.4%) NLLDSLH(3.3%) NPTHPIY(2.4%) 10 Phage display (competitive panning) 2 PMID:26246327 Plasma of peanut allergic patient 1 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3144 GASESYL(36.4%) MPTWLHH(26.1%) IENRIYR(13.4%) AMSPLNK(8.6%) FASRSDT(3.6%) NPPWFHT(2.2%) GVFISYN(2.2%) IPNGHFT(1.4%) NVRLPYQ(0.7%) NLLDSLH(0.7%) 10 Phage display (competitive panning) 3 PMID:26246327 Plasma of peanut allergic patient 1 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3145 LSANHWV(24.5%) VPNIVTQ(7.5%) VTRDSNH(7.2%) SHPLWNS(5.6%) SFNLPNT(4.1%) LPFINSD(2.5%) HTNSAYI(1.7%) GMMSSPP(1.6%) DAIPTSV(1.5%) HAGFVPS(1.4%) 10 Phage display (competitive panning) 1 PMID:26246327 Plasma of peanut allergic patient 4 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3146 VPNIVTQ(48.7%) LSANHWV(24.3%) SFNLPNT(5.1%) GMMSSPP(4.7%) YVTRTPY(1.8%) HGGVRLY(1.3%) HYIDFRW(1.2%) ATKHYTT(1.0%) AVDPQYG(1.0%) DAIPTSV(0.8%) 10 Phage display (competitive panning) 2 PMID:26246327 Plasma of peanut allergic patient 4 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3147 VPNIVTQ(75.5%) LSANHWV(5.6%) HGGVRLY(5.0%) SFNLPNT(3.4%) GMMSSPP(3.1%) YVTRTPY(1.5%) VSYGVPM(0.8%) HYIDFRW(0.7%) KGIYHQV(0.3%) AVDPQYG(0.3%) 10 Phage display (competitive panning) 3 PMID:26246327 Plasma of peanut allergic patient 4 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3148 EFARNSI(21.6%) LDIVDAP(3.6%) ALPLLDA(2.6%) FSTNFGN(2.5%) QHDTVPP(2.1%) HISGRPL(2.0%) VNGHYTI(2.0%) QIGHDGN(1.8%) GQAMNHT(1.8%) DFHPDVL(1.7%) 10 Phage display (competitive panning) 1 PMID:26246327 Sera of peanut-tolerant subject 1 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3149 EFARNSI(78.7%) QHDTVPP(6.7%) HSTQLPY(3.5%) HYIDFRW(2.2%) FVPHKWY(1.9%) AMSHSKQ(1.2%) FCPDCKP(0.8%) TGKMLAD(0.6%) GAAYIRA(0.5%) NVAHKMF(0.5%) 10 Phage display (competitive panning) 2 PMID:26246327 Sera of peanut-tolerant subject 1 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phages were eluted by adding 25 μg Ara h 1 protein. The ten most frequent peptides were shown. 3150 EFARNSI(75.7%) HYIDFRW(18.7%) QHDTVPP(1.6%) FVPHKWY(1.1%) YGNSGIV(1.0%) AMSHSKQ(0.8%) FCPDCKP(0.4%) HSTQLPY(0.1%) NVAHKMF(0.1%) TGKMLAD(0.0%) 10 Phage display (competitive panning) 3 PMID:26246327 Sera of peanut-tolerant subject 1 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) The ten most frequent peptides were shown.