276 CX6C T7 phage display library 6 1.8e9 Toshiyuki Mori Completely random NNK Circular The T7 library was constructed with the T7Select 10-3b vector. 277 fNG1 phage display library (X30) 30 Y.-C. JACK CHEN Completely random NNK Linear The vector fNG1 was derived from M13tsp3. The vector M13tsp3 is a derivative of M13mpl8 having inactivated the a-complementing fragment of lacZ of M13mpl8 and to which the Ap(R)-conferring gene was added. The Ap(R) marker aids in identification of transformants and applies a selective pressure for maintaining the vector in the cells. 278 X8 fAFF1 phage display library 8 1.4e9 W. Dower (Affymax) Completely random NNK Linear The library is based on a filamentous bacteriophage affinity vector, fAFF1, which was constructed from the tetracycline resistance-transducing vector fd-tet. Random peptides were express on protein III. Coding scheme: NNK, where N represents equimolar A, C, G, or T and K is equimolar G or T. NH2-XXXXXXXXGG=TVESC, amino acids after the equals sign belong to the native gene III protein. 279 SGTACX(2)GPX(4)CSLAGSP M13 phage display library 20 1.8e8 Henry B. Lowman Semi-random NNS Circular 280 X(4)CX(2)GPX(4)CX(4) M13 phage display library 18 7.9e8 Henry B. Lowman Semi-random NNS Circular 281 X20 M13 phage display library 20 5.0e8 Henry B. Lowman Completely random NNS Linear 282 T7 CX7C phage display library 7 Erkki Ruoslahti Completely random NNK Circular 283 CX(2)GPX(4)C, X(4)CX(2)GPX(4)CX(4), and X(i)CX(j)CX(k) M13 phage display library pool 8, 18, 20 Mark S. Dennis Semi-random NNK Linear For X(i)CX(j)CX(k) phage display library, j=8-10, i+j+k=18 and |i-k|<2. 284 X20 and X(i)CX(j)CX(k) M13 phage display library pool 20 Mark S. Dennis Semi-random NNK Linear For X(i)CX(j)CX(k) phage display library, j=4-7, i+j+k=18 and |i-k|<2. 285 X8 and X(2)CX(j)CX(2) M13 phage display library pool 8, 10-12 Mark S. Dennis Semi-random NNK Linear For X(2)CX(j)CX(2) phage display library, j=4-6. 286 X(2)CX(j)CX(2) M13 phage display library 13-16 Mark S. Dennis Semi-random NNK Circular For X(2)CX(j)CX(2) phage display library, j=7-10. 287 X(i)CX(j)CX(k) M13 phage display library pool 20 4.0e9 James A. Wells Semi-random NNS Circular For X(i)CX(j)CX(k) phage display library pool, i+j+k=18. 288 f8 α phage display library (XXLXXXAX) 8 V. A. Petrenko and G. P. Smith Semi-random NNK Linear The alpha library is a new type of phage display library, in which biological selection helps to generate a great variety of conformationally biased α-helical ligands. The library was constructed in the vector f8–5 (GenBank Accessions bankit439334) 289 X15 PC89 phage diaplay library 15 Stefan Wagner Completely random NNN Linear 290 CX7C, CX8C, and CX9C FUSE5-based phage display library pool 7-9 Mikhail G. Kolonin Completely random NNK Circular Random peptide libraries were constructed in the bacteriophage vector fUSE5. 291 T7 phage display library Kei-ichi Takata Completely random Linear 292 X15 PC89 phage display library 15 Tamara Men????ndez (Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana, Cuba) Completely random Linear 293 X7 T7 phage display library 7 5.0e8 Erkki Ruoslahti Completely random Linear 294 IR20 phage display library (X20) 20 4.0e7 Kathlynn C. Brown Completely random NNS Linear A new library of random 20-mer peptides in a phage vector was created by cloning the sequence into bacteriophage vector fAFF-1. A library of 4.0e7 independent clones encoding 20-mer peptide sequences embedded between the BspEI and Acc65I cloning sites of fAFF-BA was constructed. This 20-mer library, referred to as IR20, differs from the original library in that the cloning region within the bacteriophage vector has been modified. This allows for efficient directional cloning and results in the addition of Ser-Gly to the N-terminus of the peptide-pIII fusion. In the previously used library, the 20-mer library is located directly at the N-terminus of the fusion, and it is possible that modification of the N-terminal amino group of the peptides may disrupt cell targeting. 295 X12 phage display library 12 Han-Chung Wu Completely random Linear 296 XXCX4CXX phage display library 10 3.0e8 Chi-Bom Chae Semi-random NNK Circular Random nucleotides were introduced into the NH2-terminal region of the pⅢ gene of the pCANTAB5E phagemid using SfiI and NotI sites. Each phage displays an octapeptide epitope containing a disulfide bond as fused to the NH2 terminus of pⅢ coat protein in M13 phage and contains a factor Xa cleavage site between an epitope and pⅢ coat protein. 297 pGWX3YX4 phage display library 8 1.1e8 Su-Jun Deng, Gaochao Tian Semi-random NNK Linear The library was constructed in the vector pGWg3. To construct phage display vector pGWg3, EcoRI- and HindIII-digested pUC18 was ligated with the M13 phage gIII fragment after being amplified using PCR with oligonucleotides 5’-GGGGAATTCA AACTCAGATC TGGTGGCGGT GGATCCCCAT CGTTTGTGAA TATCAAGGC-3’ and 5’-GCCAAGCTTC TAATAATAAC GGAATACCCA AAAGAACTG-3’. Subsequently, the small PvuII fragment of the ligated product that contained gIII was inserted into the large fragment of PvuII-digested pBluescript. A DNA fragment encoding Pel B leader peptide and multiple cloning sites was constructed by a synthetic ligase chain reaction approach using 4 oligonucleotides 5’-AATTGAATAG AGGGTAGAAT TGAAATACCT GCCGACCGCT GCTGCTGGTC TGCTCCTCGC GGCCC-3’, 5’-AGCCGGCCAT GGCCCAACTC GAGTGGGCGG CCGCAGGTGG ATAACAGAAA CTGATCTCCG AAGGGTACCA AGACCTGAAC A-3’, 5’-GATCTGTTCA GGTCTTGGTA CCCTTCGGAG ATCGTTTCT GTTATCCACC TGCGGCCGCC CACTCGAGTT GGGCCA-3’, and 5’-TGCCGGCTGG GCCGCGAGGA GCAGACCAGC AGCAGCGGTC GGCAGCAGGT ATTTCATCAA TTCTACCCTC TATTC- 3’. Insertion of this DNA fragment into the EcoRI- and BglII-digested pBluescript containing gIII yielded the phage display vector, pGWg3. 298 pGWX4YX4 phage display library 9 1.75e8 Su-Jun Deng, Gaochao Tian Semi-random NNK Linear The library was constructed in the vector pGWg3. To construct phage display vector pGWg3, EcoRI- and HindIII-digested pUC18 was ligated with the M13 phage gIII fragment after being amplified using PCR with oligonucleotides 5’-GGGGAATTCA AACTCAGATC TGGTGGCGGT GGATCCCCAT CGTTTGTGAA TATCAAGGC-3’ and 5’-GCCAAGCTTC TAATAATAAC GGAATACCCA AAAGAACTG-3’. Subsequently, the small PvuII fragment of the ligated product that contained gIII was inserted into the large fragment of PvuII-digested pBluescript. A DNA fragment encoding Pel B leader peptide and multiple cloning sites was constructed by a synthetic ligase chain reaction approach using 4 oligonucleotides 5’-AATTGAATAG AGGGTAGAAT TGAAATACCT GCCGACCGCT GCTGCTGGTC TGCTCCTCGC GGCCC-3’, 5’-AGCCGGCCAT GGCCCAACTC GAGTGGGCGG CCGCAGGTGG ATAACAGAAA CTGATCTCCG AAGGGTACCA AGACCTGAAC A-3’, 5’-GATCTGTTCA GGTCTTGGTA CCCTTCGGAG ATCGTTTCT GTTATCCACC TGCGGCCGCC CACTCGAGTT GGGCCA-3’, and 5’-TGCCGGCTGG GCCGCGAGGA GCAGACCAGC AGCAGCGGTC GGCAGCAGGT ATTTCATCAA TTCTACCCTC TATTC- 3’. Insertion of this DNA fragment into the EcoRI- and BglII-digested pBluescript containing gIII yielded the phage display vector, pGWg3. 299 T7 phage display library Dennis E Hallahan Completely random Linear 300 X15CX phage display library 17 J. K. Scott (Simon Fraser University, Burnaby, Canada) Semi-random NNK Linear The library was based on PC89 vector.