276 NLLPTYTPLKLM HSNLPTKRPTSL CPNGLLGPCPSL KVWSLEPPGPAA TQPLGLLPSRHL IPHHHTLNMESH KPPSTWPQNALH LETRTTPPAKSQ KLVDESSTSPLS AGMATSRSTSPL KHVPVHSALSVN LEPQINSVGMVR 12 Phage display (common panning) 3 PMID:16414071 Protein tonB Ferrichrome-iron receptor 2GRX, Not determined. Ph.D.-12 phage display library (X12) 277 CILAALSAC CLPTGGLLC CLTRSPAAC CKSAFLPWC CTKPLTQQC CLAALKSTC 6 Phage display (common panning) 4 PMID:16414071 Protein tonB Ferrichrome-iron receptor 2GRX, Not determined. Ph.D.-C7C phage display library (CX7C) 278 TMGFTAPRFPHY KLVDESSTSPLS 2 Phage display (common panning) 3 PMID:16414071 Protein tonB Vitamin B12 transporter btuB 2GSK, Not determined. Ph.D.-12 phage display library (X12) 279 CTKPLTQQC CTGPLPNRC CSPRTTPFC CMLEKPRLC CNQPRGPQC CLTQTPTRC 6 Phage display (common panning) 4 PMID:16414071 Protein tonB Vitamin B12 transporter btuB 2GSK, Not determined. Ph.D.-C7C phage display library (CX7C) 280 KVWSLEPPGPAA AALGTYSTHTPT SSMKVWSLPPAP NLLPTYTPLKLM NNSQKPAPVSPF GNSVNKTWTHDY KHVPVHSALSVN 7 Phage display (common panning) 3 PMID:16414071 Protein tonB Fe(3+) dicitrate transport protein fecA Not determined. Not determined. Ph.D.-12 phage display library (X12) 281 CLTQTPTRC CHATLPPTC CSWDPAPLC CTLSPKLHC 4 Phage display (common panning) 4 PMID:16414071 Protein tonB Fe(3+) dicitrate transport protein fecA Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 282 SLKNYPVSWKNT NLLPTYTPLKLM TQPLGLLPSRHL HSNLPTKRPTSL QSPVNHHYHYHI KLVDESSTSPLS SHSNTTQTRPSD 7 Phage display (common panning) 3 PMID:16414071 Protein tonB Ferrienterobactin receptor Not determined. Not determined. Ph.D.-12 phage display library (X12) 283 CTGPLPNRC CSWDPAPLC CSHFAPHQC CSQVPLKSC CHMSPLGAC CMHMAPLSC 6 Phage display (common panning) 4 PMID:16414071 Protein tonB Ferrienterobactin receptor Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 284 INVPMMVDGITM(2) RGYPVVDWIFME(1) INIDGVAMDIGM(1) 3 Phage display (common panning) 2 PMID:16414054 Anti-tenascin-C monoclonal antibody ST2146 Tenascin,TN Not determined. Not determined. X12 phage display library 285 WHWLQWPQTPQP WHWQWTPWSIQP WHWSLWRPPYTL WHWTNWGRTSPA WPWWKNPNPNPT VHWWWNPPPPPI FHWPYYPAYWAL YPWHWWHSVSPW HNYRIWNHWWLS W-H-W-x(0,2)-W 9 Phage display (common panning) 3 PMID:15788194 Anti-MC-LR monoclonal antibody MC10E7 Microcystin-LR, MC-LR Not determined. Not determined. Ph.D.-12 phage display library (X12) 286 CQFSAQSRC CLATSGQPC CTNQPRSFC CQPHPYYMC CSLSLTRHC CLTNSYNTC CSEMRLALC CPEHNLRFC 18 Phage display (common panning) 3 PMID:15788194 Anti-MC-LR monoclonal antibody MC10E7 Microcystin-LR, MC-LR Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 287 ASPVTARVLWKASHV VSSLVLLSHGGPHSS IMVLCPLWLWLGTTS AVAHVTSRVPRWSAA 4 Phage display (common panning) 3 PMID:16290070 Anti-dsDNA polyclonal antibody Deoxyribonucleic acid, DNA Not determined. Not determined. f3-15mer phage display library (X15) 288 MSTRPPT(2) LAHTTQP(1) LTLQPLS(1) MQPWSLA(1) LATIPAS(1) LAKFPVA(1) LGKFPYP(1) LAKQPPR(1) LATLPKN(1) LTVLPQN(1) LASLPRV(1) LSLPSLY(1) MSLPLLK(1) MSLPFSG(1) MSTPPVT(1) MSKLPFN(1) MSALPSA(1) MQILPAS(1) MAVPTSI(1) LARTLTT(1) LQTFQNV(1) MASSVST(1) MAISPSS(1) MQIPTGY(1) MAAPTGY(1) MAAPIEH(1) MAIPRAA(1) MAHPGVS(1) MASPLFS(1) LASPPFY(1) FSMRIPI(1) MTIPITP(1) MGQPISP(1) MTTPQIR(1) MTMPVMA(1) MTLPPEA(1) LSRPPAA(1) MTLPMDH(1) FSRPAIA(1) LSHNPAP(1) LSHIPET(1) MQLLPPP(1) MTLTPPP(1) LTTPPRL(1) LTVPPAL(1) LTTPPFI(1) LTPPPRV(1) LTLPPRT(1) LTRPPMP(1) MASYGPG(1) LASRGPM(1) MASRTHP(1) MANRPHP(1) GTLLSRT(1) MTALPLR(1) MTSIPRN(1) LTWRMRS(1) MSTAWQL(1) MTTALSL(1) MTSATLS(1) YTSARLF(1) MTSTAPL(1) LTPVSVF(1) LTPMRTY(1) LQPPWLT(1) LTPSWLP(1) VPRLLPL(1) FAAMPAL(1) SNYNTQY(1) MYAIRTT(1) LSGPVKH(1) SGQAAVQ(1) LSRPPSQ(1) MTVPPSV(1) 74 Phage display (subtractive panning) 5 PMID:15949645 Endothelial protein C receptor Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) The phage library was incubated on four successive cultures of untransfected HepG2 cells in 1ml of biopanning media. Incubation of the library on untransfected HepG2s should effectively remove from the phage population those bearing peptides capable of binding receptors normally expressed on HepG2, thus clearing the phage library of phage able to bind to non-target receptors. Cleared phage library was then panned immediately in duplicate on HepG2-EPCR. Cells were extensively washed, weakly associated phage removed by acid wash prior to recovery of high affinity phage isolated by cell lysis. Four additional rounds of panning were performed including a clearing step on HepG2-beta-gal prior to biopanning on HepG2-EPCR. 289 WHWSLWRPPYTL(4) FHWRWPPSLQIM(2) FHRTPWLQFSLP(1) LHRPHLYHVWQR(1) WHWSWIQNAAPN(1) WHWSYASQLLQI(1) 6 Phage display (common panning) 4 PMID:15963509 Pesticidal crystal protein cry11Aa Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 290 CDGGWLSKGSWC[0.876 ± 0.078] CSGAWLSSGRWC[1.177 ± 0.054] CDPRALDYWLRC[1.045 ± 0.050] CVGDARLWWRDC[1.012 ± 0.022] CTSWLNEGRASC[0.887 ± 0.045] CGRLKMVPDLEC[1.219 ± 0.035] CGRLRMVPDLEC[1.166 ± 0.066] CIRESGMSAGEC[0.788 ± 0.032] CIRGFAMSAGDC[1.094 ± 0.038] CIRSSPLLPGDC[1.445 ± 0.030] CLRGDSLTPGDC[1.324 ± 0.128] CVRTSSMVPGDC[0.981 ± 0.087] CDPGWLSKGTWC[0.989] 13 Phage display (common panning) 3 PMID:15589320 Anti-GD2 monoclonal antibody ch14.18 Disialoganglioside GD2 Not determined. Not determined. CL10 phage display library (CX10C) ELISA Optical density was quantified in an ELISA reader (Dynatech, Denkendorf, Germany) at 405–409 nm. Data shown were reproduced from the graph. 291 PRRIKPRKIMLQ(53) 1 Phage display (common panning) 6 PMID:15919139 Anti-tumor monoclonal antibody BAT Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 292 QRILQQINLPRI(31) 1 Phage display (common panning) 3 PMID:15919139 Anti-tumor monoclonal antibody BAT Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) In the screening, the phage display peptide library was subjected to three rounds of affinity selection. Each affinity selection was done by biopanning on Petri dishes coated with streptavidin. Petri dishes coated with streptavidin were blocked with phosphate buffered saline (PBS) containing 3% bovine serum albumin (BSA) and 0.1 mg/ml streptavidin. 293 SHGFSRHSMTLI SVSVGMKPSPRP AFSNTHRMMPLK NHTTELGSLMPG 4 Phage display (common panning) 5 PMID:16029812 Human pancreatic adenocarcinoma cell line Capan-2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 294 PKWRHRY FNENWPR WDGNYTL RWRSNEP 4 Phage display (common panning) 3 PMID:15757647 C-C motif chemokine 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 295 HTRMHWHHVHTL HNWKHTHLKTPA SLWHGHKHHSPL HNWHWHSIRGRR SWKHHNFHSRYS WPHHHRSQRVAS KRFHPMHHTIRH HRPHYHTDYLTA SWPHHHRMPRLA 9 Phage display (common panning) 3 PMID:15757647 C-C motif chemokine 2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 296 AWLPWAK(4)[0.384 ± 0.010][272] NLQEFLF(2)[0.376 ± 0.022][NT] WMKPIW(1)[0.214 ± 0.010][NT] TYFGDWF(1)[0.225 ± 0.036][NT] MPPLWWS(1)[0.192 ± 0.010][NT] FVRPFAL(1)[0.186 ± 0.024][NT] 6 Phage display (common panning) 5 PMID:15721308 Lipopolysaccharide, LPS Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA,Surface plasmon resonance (SPR) Phage clones were allowed to react with beads conjugated with LPS extracted from S. enteritidis. The beads were exposed to horseradish peroxidase conjugated anti-M13 antibody and then ABTS substrate solution sequentially with an intermittent washing step. M13KO7 helper phage was used as a negative control. Unconjugated epoxy beads were employed to determine the background signal level. The optical densities were measured at 405 nm. Data shown are mean values of triplicate experiments and their standard deviations, and reproduced from the graph. Besides, the binding characteristics of the phage displaying AWLPWAK for the LPS of S. enteritidis were examined by surface plasmon resonance (SPR) analysis. The calculated kon, koff, and KD (KD=koff/kon) values were 1.68e6 1/Ms, 0.0457 1/s and 272 μM, respectively. NT, not tested. 297 HLNILSTLWKYR(35) TTVYPARWGAHP(3) SYQLSTHRWPLH(2) NHVHRMHATPAY(2) 3 Phage display (common panning) 4 PMID:16023583 Trisialogangliosides (GT1b) Tetanus toxin Not determined. Not determined. X12 phage display library Peptides specific for GT1b were isolated using a four-round biopanning strategy. The initial round of biopanning was carried out in triplicate, eluting bound phage with a nonspecific acidic elution. This stringent elution was performed to prevent the loss of strong-binding phage clones in early rounds. Also, to optimize the capture of GT1b binding phage clones, eluates from the three separate biopanned wells were combined and amplified for use in the second round of biopanning. In the second through fourth rounds of panning, the bound phage was eluted with the tetanus toxin C fragment (rTTC) to specifically isolate phage peptides that mimic tetanus toxin binding properties. 298 CEHGAMEIC CGGGRPASC CHLGVEHLC CEEKEPERC 4 Phage display (common panning) 1 PMID:15707681 Anti-EGF monoclonal antibody EGF-10 Pro-epidermal growth factor, EGF Not determined. Not determined. CX7C phage display library 299 DHYIYEEIY(9) DDFEYSYHN(1) DPDIYDYWY(1) DLGYYYFGR(1) NPDIYEEIY(1) 5 Phage display (common panning) 3 PMID:14715298 Src SH2-GST fused protein Not determined. Not determined. Not determined. X4YX4 phage display library 300 ELDEYMEYE(5) KLAAYFEYL(2) SQVCYSGYP(2) PIRCYARGS(1) PQSSYVAIS(1) VDYVYVSSE(1) 6 Phage display (common panning) 3 PMID:14715298 PTP-alpha-GST fused protein Not determined. Not determined. Not determined. X4YX4 phage display library