2951 AELAKLPLF GAISFDHVHP 2 Phage display (subtractive panning) 6 PMID:26543513 Colorectal cancer cell(HCT-8) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection using magnetic beads to capture M13 phage display peptides that were not of interest was first performed such that peptides binding on non-target cells (control cells) could be removed. Then, the supernatant was collected to perform the subsequent positive selection. As mentioned previously, the target cells surfaced-coated with magnetic beads were incubated with the supernatant to perform the positive selection. 2952 MNAKWPIENLMN 1 Phage display (subtractive panning) 6 PMID:26543513 Cancer stem cell(CSCs) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection using magnetic beads to capture M13 phage display peptides that were not of interest was first performed such that peptides binding on non-target cells (control cells) could be removed. Then, the supernatant was collected to perform the subsequent positive selection. As mentioned previously, the target cells surfaced-coated with magnetic beads were incubated with the supernatant to perform the positive selection. 2953 SVACHSTKAHSC RVACLYHTTRAC VCACLYHTTRAC VCACNWNNHRAC FTACNWNNHRAC RRACQSHKARSC 6 Phage display (common panning) 3 PMID:26438313 Human squalene synthase(hSQS) BPH701 (1-phospho-4- (3-(4-pyrolphenoxy) phenyl) butane-1-sulfonic acid Not determined. Not determined. Ph.D.-12 phage display library (X12) 2954 DHIHRSYRGEFD(3/16) NIYTTPWGSNWS(2/16) SHSLPASADLRR(2/16) SAAYLAVIDTSS(1/16) HSPQYWVHHWRG(1/16) GQSEHHMRVASF(1/16) YQFGYDYPRSQV(1/16) WSGPAVMLETFF(1/16) ERLSDFANFRAP(1/16) HYPFMTPVLFTP(1/16) SALKGLFPADHH(1/16) AAFPSLNLRTQP(1/16) 12 Phage display (common panning) 3 PMID:26521981 Beta-lactamase 2 (EC:3.5.2.6) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2955 ALWPPNLHAWVP(9) AHSANNFNVKGI(3) FTTKTYNGVAER(2) ALWPPNPQALVP(1) NHSVNNLNVKGI(1) VALKAWAHPTHW(1) FPRNVANSPVYN(1) YSAHNYIGNSGP(1) HVVKQAMSNNMM(1) RFWPPNLQAGVP(1) RLNASSMISGRV(1) 11 Phage display (common panning) 3 PMID:26482679 Anti-DNA polyclonal antibody IgG1 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2956 ALWPPNLHAWVP(5) TPMVERNYNAAN(3) RLNASSMISGRV(3) AHSANNFNVKGI(2) ALWPPIPHAWVH(1) KLLLPIPQVGPL(1) KLLSPNLQAGVL(1) KLLFAIPEVEGF(1) RLNNSSMNSGRV(1) NWNNPPIRLVAS(1) YSAHNYIGNSGP(1) NSVHTNTGNLGL(1) TPPNNYYYVEAV(1) IGSPHLTAPLLT(1) WHSWAMHQNEHF(1) HNGGAMHIQKMK(1) HVLYTIAGGLEP(1) LNWTPASTSLKN(1) NQAQFELLNKVK(1) 19 Phage display (common panning) 3 PMID:26482679 Anti-DNA polyclonal antibody IgG2a Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2957 ALWPPNLHAWVP(11) ALWPPNLQAWAP(1) ALWPPNLQAGVP(1) KLWLPNLQAGVP(1) AFYPNNTVSRLA(1) AHSANNFNVKGI(1) NHLANNFNNKGI(1) THNFRFLMTLGW(1) TNQNYTSLPVAA(1) HVVKQAMSNNML(1) HVVKQAMFHNMM(1) VGANWSSYHVLN(1) NNLNYTSNPVAH(1) NSNWKTFGGWPS(1) YNHSATHLVSLP(1) TPTSTLHKFKHW(1) SSPPISMGSVGG(1) RIILRVLLRCIR(1) 18 Phage display (common panning) 3 PMID:26482679 Anti-DNA polyclonal antibody IgG2b Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2958 ALWPPNLHAWVP(16) AHSANNFNVKGI(6) HVVKQAMSNNMM(4) KNMAKAENNVYM(3) SCTGLLSYHSRC(3) VHLQAGELMNPK(2) AHSAANFNVKGW(1) SYSALNHPPHPT(1) NLSHFLPLSAPV(1) NYEFSISEQSHN(1) AETVESCLAKPH(1) APTTQRVFWNQS(1) 12 Phage display (common panning) 3 PMID:26482679 Anti-DNA polyclonal antibody IgG3 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2959 SWQIGGN(6)[1.10 ± 0.11] IGNSNTL(2)[1.08 ± 0.09] QFHFDAP(1)[1.22 ± 0.12] TSPFVVP(1)[1.13 ± 0.07] TGNSNTQ(1)[1.24 ± 0.13] TSHFEVP(1)[1.20 ± 0.05] 6 Phage display (subtractive panning) 4 PMID:26555399 Human ovarian cancer cell line HO8910 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) ELISA The binding activities of the positive phage clones to human ovarian cancer cell line HO8910 were measured by phage ELISA. The OD values at 450 nm were measured and data shown were reproduced from the Fig. 1 in the reference. M13KE was used as controls and had the affinity value 0.19 ± 0.02 with this target. Biopanning began with the incubation of the phage display peptide library with both normal and tumor cells, in which normal cells were used to deplete peptides that only bind to normal cells and then further incubated with tumor cells for identifying the peptides that only specifically bind to tumor cells. Peptide1 read as SWQIGGN was the positive peptide and showed preferential binding to the target cells. Peptide 1 also inhibited cell proliferation, migration, invasion and adhesion of ovarian cancer HO8910 cells in vitro. 2960 THENWPA(16/20)[1.15 ± 0.12] APQWPAN(3/20)[0.77 ± 0.11] DVTSNFP(1/20)[0.74 ± 0.06] 3 Phage display (subtractive panning) 4 PMID:26423339 CD44v3-v10 glycoprotein Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA The binding activities of the positive phage clones to CD44v3-v10 glycoprotein were measured by phage ELISA. The OD values at 450 nm were measured and data shown were reproduced from the Fig. 2a in the reference. Data are presented as the mean ± SD. Unrelated phages randomly selected from the original library served as the negative control and had the affinity value 0.27 ± 0.03 with this target. The library was incubated with the CD44s to eliminate phages binding to CD44s. Next, unbound phages were incubated with CD44v3-v10 and bound phages were eluted with 0.2 M glycine-HCl (pH 2.2) supplemented with 1% (w/v) BSA. 2961 NTWHNSR(12/34)[0.33 ± 0.03] WHKEQFW(9/34)[0.12 ± 0.02] QTKMTNP(2/34)[0.20 ± 0.02] TVKTRPA(2/34)[0.29 ± 0.02] AGPTRIS(2/34)[0.11 ± 0.05] THSPGLL(1/34)[0.23 ± 0.02] SINPDNR(1/34)[0.20 ± 0.02] ALLADSR(1/34)[0.22 ± 0.04] EIALGAR(1/34)[0.22 ± 0.03] ATSAIHG(1/34)[0.14 ± 0.02] HVQLLIF(1/34)[1.89 ± 0.03] SITTVAA(1/34)[0.13 ± 0.02] 12 Phage display (competitive panning) 4 PMID:26577672 Immunoglobulin G (IgG) of Tuberculosis (TB) patients Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA The binding activities of the positive phage clones to IgG from TB patients were measured by phage ELISA. The A450 values were measured and data shown were reproduced from the Fig. 1a in the reference. Data are presented as the mean ± SD. The first eluted phage library was used as negative control and had the affinity value 0.39 ± 0.03 with this target. Bound phage was eluted with free purified IgG from TB patients to compete the bound phage away from the immobilized antibodies on the plate. 2962 TTAGIRQ(19/34) SNAPQKL(8/34) FHFPPVY(2/34) WHLPLSL(1/34) ANVWMPT(1/34) LPPPPPFS(1/34) WHALPTN(1/34) AHGPLAF(1/34) 8 Phage display (subtractive panning) 4 PMID:26577672 Immunoglobulin G (IgG) of Tuberculosis (TB) patients Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA The binding activities of the positive phage clones to IgG from TB patients were measured by phage ELISA. The A450 values were measured and data shown were reproduced from the Fig. 1b in the reference. Data are presented as the mean ± SD. The first eluted phage library was used as negative control and had the affinity value 0.06 ± 0.01 with this target. The library was added onto the healthy IgG coated plate first, and the unbound phage supernatant was added to TB patients IgG package plate. 2963 EHFSTGMG DGLRPQAP DGLRPPGS DGLRSPPG DGLRSPGS DGLRNPSS D-G-L-R 6 Phage display (common panning) 3 PMID:26456116 Anti-Gonadotropin releasing hormone (GnRH) polyclonal antibody of cat 1 Gonadotropin releasing hormone (GnRH) Not determined. Not determined. f8-8mer phage display library (X8) 2964 EHPSYGLA DHPSYTPM ETSYADHT H-x-S-Y 3 Phage display (common panning) 3 PMID:26456116 Anti-Gonadotropin releasing hormone (GnRH) polyclonal antibody of cat 2 Gonadotropin releasing hormone (GnRH) Not determined. Not determined. f8-8mer phage display library (X8) 2965 DAPAHWSQ EPTSHWSA EPSPHWSS ETTSHWSS DDTHWAAP EDYGARPA DSDYGARP DTDYGARP EDSYGERP ESASYGER ESVSYGAK ESASYGER ESDYGARV EEYGARST EEYGMRGP EAGYGEK H-W-S, Y-G-x-[RK]-P 16 Phage display (common panning) 3 PMID:26456116 Anti-Gonadotropin releasing hormone (GnRH) polyclonal antibody of dog Gonadotropin releasing hormone (GnRH) Not determined. Not determined. f8-8mer phage display library (X8) 2966 EGLRPSGA EGLRPSNA DGLRPSNS EGLRPSGQ EGLRPSGS EGLRPTGS EGLRPSGQ [ED]-G-L-R-P-S 7 Phage display (common panning) 3 PMID:26456116 Anti-Gonadotropin releasing hormone (GnRH) polyclonal antibody of rabbit Gonadotropin releasing hormone (GnRH) Not determined. Not determined. f8-8mer phage display library (X8) 2967 SYLPETIYEYRL(26) VENKTRYHDREV(7) HEGAWHNYARSV(1) 3 Phage display (common panning) 3 PMID:26709700 FvTox1 protein Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2968 SNGRVAD(1) 1 Phage display (common panning) 3 PMID:26709700 FvTox1 protein Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2969 RMLPASLHL RRLPEELHR ARLPPSLHL RNLPRALSP RRLPSAFHP LRSLHLVRG SRPPSLFRP RNVFLIRTE GRVFFLVRP DRNIYLVRQ 10 Phage display (common panning) 2 PMID:12943796 Anti-HBX monoclonal antibody Recombinant HBX protein Not determined. Not determined. pVIII-9aa phage display library (X9) 2970 LSLTSTL LPTPALR ASGLLSL SLTSTLY TLLVQGS LPLNSSA LSLPLNS SSSLTLK VTSALSR SALSRDG RLSVPPL GLLSLTS GSPSTPA LSLSSIT SLTLKVT KVLSLPL SLPLNSS LSVPPLV 18 Phage display (common panning) PMID:27143691 Human KRAS-mutant colorectal cancer cell lines and tissues Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) The ex-vivo screenings were run on pooled specimens from different animals (n=3), with a standard procedure that involved two successive selection rounds (R1, R2) and two alternative controls (C1, pseudo-metastasis from WT cells; C2, non-tumoral liver). The in vitro screenings were performed with the same standard procedure (P1) or with an enrichment protocol (P2) in which several rounds were iterated until increased phage binding to KRAS mutant vs WT cells was observed. Each KRAS mutational status (G12D, G12V, G13V) was independently screened in 4 biological replicates, i.e. 2 cell lines (SW-48, LIM1215) × 2 clones, and in 2 experimental conditions (ex-vivo and in vitro) for a total of 24 phage display screening experiments. Each of them consisted of 5 output samples (3 mutant KRAS + 2 controls), giving a total of 120 samples. This subset of peptides has been derived from a larger, deep-seq analysis. These peptides are identical to regions of the human BCAM protein and are potential ligands for LAMA5. 2971 RRPRMLT[1.40 ± 0.23] QLRRQRR[NT] HSRRMRK[NT] RSRRIRL[5.37 ± 0.49] R-R-x-R 9 Phage display (common panning) 4 PMID:17699853 Neuropilin-1 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Surface plasmon resonance (SPR) Surface plasmon resonance was used to measure the real-time association and dissociation of the binding of RRXR-containing peptides to NRP1. The average dissociation constants (KD) for the binding of DG1 and DG2 to NRP1 were 1.40 ± 0.23 and 5.37 ± 0.49 umol/L, respectively. Two potent synthetic anti-NRP1 peptides, CRRPRMLTC and CRSRRIRLC, which block NRP1 signaling pathways and suppress tumorigenesis, cancer invasion, and angiogenesis, were identified. 2972 SPFWWSD(25) SPFWWTD(36) PNPFVLD(1) LGSVQHT(1) SPFWWSL(1) FSPFFWN(1) WILFFLD(1) NSPFQLF(1) PGLATNF(1) 9 Phage display (subtractive panning) 3 PMID:26806845 Purified IgG from serum of juvenile idiopathic arthritis (JIA) persistent oligoarticular subtype Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) For screening the PD library to select JIA mimotopes, a subtractive step against purified IgG from healthy children and patients with other autoimmune diseases, followed bya positive selection against purified IgG from JIA subtypes was used. 2973 SPFWWHQ(3) SPFFLTP(1) WNPFLLD(1) QSPFHLF(1) NPGWLGS(1) SAVIKSS(1) SSHLLSE(1) LPSRSQT(1) NNPFQLW(1) SSFWWTD(1) 10 Phage display (subtractive panning) 3 PMID:26806845 Purified IgG from serum of juvenile idiopathic arthritis (JIA) extended oligoarticular subtype Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) For screening the PD library to select JIA mimotopes, a subtractive step against purified IgG from healthy children and patients with other autoimmune diseases, followed bya positive selection against purified IgG from JIA subtypes was used. 2974 FDPFGWS(2) SSWLPRG(1) WSPFLAP(1) SPFDWWF(1) NPFFLTA(1) SPNPPLH(1) SSPFFLW(1) SPFHLLP(1) 8 Phage display (subtractive panning) 3 PMID:26806845 Purified IgG from serum of juvenile idiopathic arthritis (JIA) polyarticular rheumatoid factor negative subtype Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) For screening the PD library to select JIA mimotopes, a subtractive step against purified IgG from healthy children and patients with other autoimmune diseases, followed bya positive selection against purified IgG from JIA subtypes was used. 2975 NPFSLLA(1) YFTAPPD(1) DSPFRLW(1) TRPTASH(1) SESPGIA(1) FSPFFAP(1) SPFWLAA(1) SPFFLGP(1) TPFWFLD(1) PAPSRSQ(1) RFGGLIA(1) 11 Phage display (subtractive panning) 3 PMID:26806845 Purified IgG from serum of juvenile idiopathic arthritis (JIA) polyarticular rheumatoid factor positive subtype Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) For screening the PD library to select JIA mimotopes, a subtractive step against purified IgG from healthy children and patients with other autoimmune diseases, followed bya positive selection against purified IgG from JIA subtypes was used.