2926 APLSPPG(6/30) APLSRPG(2/30) APLSRRG(1/30) APLPRPG(1/30) GPLSRPG(1/30) HPLMRRG(1/30) HPLIVLE(1/30) AVLRTSP(1/30) APSPMIW(1/30) HSLELMP(1/30) RLPAFLH(1/30) LMPARYH(1/30) HLWLIPP(1/30) TYASDRS(1/30) QYATPSA(1/30) QMAQWPP(1/30) SWNMWSP(1/30) HWGMWSY(1/30) VNSAWTY(1/30) TTTWHLK(1/30) NQSAVLP(1/30) DSHTPQR(1/30) GHWTRFA(1/30) GHWTRFA(1/30) 23 Phage display (common panning) 3 PMID:26369825 Amino acid sequence FDYSEPGNFSDISWPCNSSD from ACKR3/CXCR7 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2927 LLADTTHHRPWT(2/10) GQVLPTTTPSSP(1/10) MPFGARILSLPN(1/10) QLQLSNSMSSLS(1/10) SKHERYPQSPEM(1/10) HTHSSDGSLLGN(1/10) ANPIISVQTAMD(1/10) 7 Phage display (common panning) 3 PMID:26121597 Beta-tri calcium phosphate Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Beta-tri calcium phosphate powder was blocked for 24 hours at 4°C under moderate agitation with sterile filtered Odyssey Blocking Buffer (OBB; non-mammalian blocking buffer; LI-COR; Lincoln, NE, USA). Blocked Beta-tri calcium phosphate was pelleted at 2000 RPM for 2 minutes, washed 3X with PBS then subjected to three rounds of phage display. 2928 LLADTTHHRPWT(5/10) HNMAPATLHPLP(1/10) QSFASLTNPRVL(1/10) HTTPTTTYAAPP(1/10) QYGVVSHLTHTP(1/10) DYDSTHGAVFRL(1/10) 6 Phage display (common panning) 3 PMID:26121597 Beta-tri calcium phosphate Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Beta-tri calcium phosphate powder was blocked for 24 hours at 4°C under moderate agitation with 5% bovine serum albumin in phosphate buffered saline (BSA; Sigma-Aldrich, St. Louis, MO, USA). Blocked Beta-tri calcium phosphate was pelleted at 2000 RPM for 2 minutes, washed 3X with PBS then subjected to three rounds of phage display. 2929 LLADTTHHRPWT(1/10) VPQHPYPVPSHK(1/10) TMSNPITSLISV(1/10) IGRISTHAPLHP(1/10) MNDPSPWLRSPR(1/10) QSLGSMFQEGHR(1/10) KPLFTRYGDVAI(1/10) EPTKEYTTSYHR(1/10) DLNELYLRSLRA(1/10) NYDSMSEPRSHG(1/10) 10 Phage display (common panning) 3 PMID:26121597 The composite of beta-tri calcium phosphate and polylactide-co-glycolide (PLGA) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Beta-tri calcium phosphate powder combined with PLGA was blocked for 24 hours at 4°C under moderate agitation with 5% bovine serum albumin in phosphate buffered saline (BSA; Sigma-Aldrich, St. Louis, MO, USA). Blocked Beta-tri calcium phosphate was pelleted at 2000 RPM for 2 minutes, washed 3X with PBS then subjected to three rounds of phage display. 2930 APAIPAS(20/25) APALPAS(1/25) APSIPAS(1/25) 3 Phage display (common panning) 3 PMID:26172085 Anti-vibrio cholerae O1 Ogawa monoclonal antibody IXiao3G6 Vibrio cholerae serotype O1 Not determined. Not determined. Ph.D.-7 phage display library (X7) 2931 ALWPPQGHAWVP(7/10) HGENPPSGMAWV(1/10) HGVNPPSGMAWV(1/10) 3 Phage display (common panning) 3 PMID:26315775 Anti-S1 subunit of IBV Sczy3 monoclonal antibody 1D5 The spike (S) protein of the infectious bronchitis virus (IBV) Not determined. Not determined. Ph.D.-12 phage display library (X12) 2932 HIQTRLEPQWKS(8/10) HGIQTATEPQWS(1/10) HTLQTRTPPQSP(1/10) 3 Phage display (common panning) 3 PMID:26315775 Anti-S1 subunit of IBV Sczy3 monoclonal antibody 6A12 The spike (S) protein of the infectious bronchitis virus (IBV) Not determined. Not determined. Ph.D.-12 phage display library (X12) 2933 NKVIWEADWAFS(21/23) NKVIWDRDWMYP(1/23) HHSHSILQSDWF(1/23) 3 Phage display (common panning) 3 PMID:15344223 Anti-E. coli K1 monoclonal antibody HmenB3 Escherichia coli K1 bacteria Not determined. Not determined. Ph.D.-12 phage display library (X12) 2934 HKMHSHPRLTSP(6/35)[0.128] WHKAVPRWLASP(3/35)[0.543] FHKHKSPALSPV(2/35)[0.479] FHGHLKKPHWRN(2/35)[0.154] WHRHWPSHPTQK(1/35)[1.615] GLLHHKHHRSPY(1/35)[0.456] DLNYFTLSSKRE(1/35)[0.177] FHKHRISPSPST(1/35)[0.252] ATAKHLYWWRNQ(1/35)[0.656] WHKGNNVAWTKR(1/35)[0.430] WHSHMKTRTWQP(1/35)[0.543] FHSPKKNHHYYR(1/35)[0.507] HHKHSNRSPIFS(1/35)[0.224] FHKHHKSPRLFP(1/35)[0.268] AHKWYSQWLPHR(1/35)[1.461] WHHRHQPAPGGR(1/35)[1.021] FHKHSPRSPIFI(1/35)[0.371] AKLHWHKHHPLT(1/35)[<0.1] FHKHNYKSPPII(1/35)[<0.1] WHKVPRSDRMPP(1/35)[<0.1] FHKYSPPQKPVT(1/35)[<0.1] WPHFHRPPHREL(1/35)[<0.1] HLYHKNRNHIAY(1/35)[<0.1] IQRHHKPLRLRV(1/35)[<0.1] FHKHDRGRLSPP(1/35)[<0.1] SWRSRQLPETGE(1/35)[<0.1] 26 Phage display (common panning) 4 PMID:14748062 Anti-NS3 monoclonal antibody 3B1C4 Hepatitis C virus (HCV) nonstructural 3 (NS3) protein Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Affinities of the selected phage clones to anti-NS3 monoclonal antibody 3B1C4 were measured by phage ELISA. The results are expressed as the mean optical density (OD492) obtained with triplicate of phage clones tested, at the final dilution of 1.0e11 phages/ml with 3B1C4 coated on the solid phase minus the mean OD of triplicate of the same phage dilutions tested with an irrelevant mAb. 2935 KPPNPSPMSRFI(6/49) VKIPNYPPNSTS(5/49) VTIPQYSPRHPV(3/49) QKPPNPSPISPL(2/49) VKIPQHINLTSE(2/49) ISPYNTIVAKLR(2/49) KPPNPSPIEHWP(1/49) KPPNPSPLALAG(1/49) KPPNPSHLSLTW(1/49) KPPNPHPLPYES(1/49) KPPNPPPPPHDL(1/49) LKPPNPMPPAHG(1/49) VKIPNTYRLGMA(1/49) VKIPNSGTALSR(1/49) VKIQNNPPPLPQ(1/49) VKINNSSPLPTG(1/49) VKIQNLPTLNTK(1/49) VKIPQFLASPLA(1/49) VKISQYASMPPI(1/49) VKIPQHMHPLPI(1/49) AKIDNAVPQTVS(1/49) LKIPNSPSRTVG(1/49) LKIPSKFSPAML(1/49) VRIPNPPPTPFL(1/49) VRIANHPPEPFR(1/49) IRIPQHDWPSGM(1/49) VTIPQYSTPPSV(1/49) VTIANNPIFTSV(1/49) ITIPQKQEYAYT(1/49) VSISNLGPQYNT(1/49) VSIPNTWPSTKT(1/49) VMISNDSKGRWW(1/49) DINLVYNTVLNK(1/49) KPPNPRPTMSLW(1/49) TKPPNPKPSMFF(1/49) 35 Phage display (subtractive panning) 3 PMID:15272409 Serum immunoglobulin (Ig) G antibodies from patients with SARS Human SARS coronavirus Not determined. Not determined. Ph.D.-12 phage display library (X12) To enhance selection of peptides binding to IgG specifically associated with patients with SARS, we designed a preclearing step to remove nonspecific clones by preabsorption of the phage-displayed 12-mer peptide library onto purified IgG from normal serum pooled from 5 control subjects. 2936 LTANTKSMALSG(20/35)[0.44] AQLPIPRWNFMS(4/35)[0.40] STLAKHAPAGYR(2/35)[0.43] LTTDHSTTVING(2/35)[0.47] VPPNTKSMALSG(1/35)[0.47] LTTHTKSMALSG(1/35)[0.40] VPANIKSMALSG(1/35)[0.37] VAPSMAkSIPAK(1/35)[0.51] AHLHIPRWNLMS(1/35)[0.24] KTNNSYIPPLTW(1/35)[0.53] ISQSLMPLSPWR(1/35)[0.51] T-K-S-M-A-L-S-G, I-P-R-W-N-[FL]-M-S 11 Phage display (common panning) 4 PMID:15607634 Anti-burkholderia pseudomallei protease polyclonal antibody IgG Burkholderia pseudomallei protease Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Affinities of the positive phage clones to burkholderia pseudomallei protease polyclonal antibody IgG were measured by phage ELISA. The A405 values were measured and data shown were reproduced from the Fig. 3a in the reference. wild-type M13 phage was used as control, which had the value of 0.27 when that phage binded to the target. 2937 ATTVPAS FNQNPRS SVLTLET TLMLRET RPPTRYH KAWIIPA 6 Phage display (competitive panning) 3 PMID:16037945 Anti-LKM1 autoantibodies from hepatitis C–infected patient 1 Cytochrome P450 2D6 (CYP2D6) protein Not determined. Not determined. Ph.D.-7 phage display library (X7) The retained phages, eluted with CYP2D6 fusion protein and purified, were then sequenced. 2938 RALSMPP STVMTRP YSPLTLI FPLHLGV TSQPTHT SSTHSAP STQHGPQ 7 Phage display (competitive panning) 3 PMID:16037945 Anti-LKM1 autoantibodies from hepatitis C–infected patient 2 Cytochrome P450 2D6 (CYP2D6) protein Not determined. Not determined. Ph.D.-7 phage display library (X7) The retained phages, eluted with CYP2D6 fusion protein and purified, were then sequenced. 2939 ESLPTFA AIDTRPW SYHSDNN NYNWTTP QVMPYVQ GPHPRSV 6 Phage display (competitive panning) 3 PMID:16037945 Anti-LKM1 autoantibodies from hepatitis C–infected patient 3 Cytochrome P450 2D6 (CYP2D6) protein Not determined. Not determined. Ph.D.-7 phage display library (X7) The retained phages, eluted with CYP2D6 fusion protein and purified, were then sequenced. 2940 QDQRTFT LVTHTPI VPATLFR PHLHPPR MSLHHSH ALRTASS HAIYPRH RPPPTAT 8 Phage display (common panning) 3 PMID:26640539 Duck hepatitis B virus polymerase (DHBVP) Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2941 CPTSHHRAC(8) CKQPSHSAC(2) CTANSSAQC(2) 3 Phage display (in vivo) 4 PMID:26568284 Sheep intestinal lymph Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Phages preselected by 2 rounds of in vitro panning were amplified and further subjected to 2 rounds of in vivo panning procedure in sheep. 2942 CTANSSAQC(12) 1 Phage display (in vivo) 4 PMID:26568284 Cells from sheep lymph plasma Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Phages preselected by 2 rounds of in vitro panning were amplified and further subjected to 2 rounds of in vivo panning procedure in sheep. 2943 STVMDSAVIVKS(8) 1 Phage display (in vivo) 4 PMID:26568284 Epithelial layer overlaying Peyer’s Patches (PP) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Phages preselected by 2 rounds of in vitro panning were amplified and further subjected to 2 rounds of in vivo panning procedure in sheep. 2944 CDPSPSRHC(1) 1 Phage display (in vivo) 4 PMID:26568284 Epithelial layer overlaying Peyer’s Patches (PP) Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Phages preselected by 2 rounds of in vitro panning were amplified and further subjected to 2 rounds of in vivo panning procedure in sheep. 2945 SNLSWPANMKHP(23/63) TWHHSYFNSVSV(22/63) ATTPNDNDLNRW(3/63) FHESWPSMSSAA(3/63) RDPYTLIQYLSV(2/63) MVFPKDGREAKL(2/63) WNAPPMINRMST(1/63) TWFEALRLNTDM(1/63) VETPTLRYSHWQ(1/63) TGHHSYFNSVSV(1/63) EYSARVQYLQFR(1/63) HAGQKDLLSAWM(1/63) VDLDFRSLILVQ(1/63) NMTPFFEGVIFN(1/63) 14 Phage display (common panning) 3 PMID:26624919 Anti-pkMSP-142 antibody Merozoitesurface protein-142(MSP-142) Not determined. Not determined. Ph.D.-12 phage display library (X12) 2946 APDTKTQ(14)[0.96 ± 0.02] ATRQPNH(6)[0.83 ± 0.10] HASKQLL(5)[0.71 ± 0.13] SPRVGAT(4)[0.72 ± 0.14] LSLSTTS(4)[0.70 ± 0.16] HLHAHKL(4)[0.74 ± 0.11] RHFHFPA(2)[0.79 ± 0.10] HPMSAPR(1)[0.81 ± 0.09] 8 Phage display (common panning) 4 PMID:26496918 Human soluble receptor for advanced glycation end products (sRAGE) Amyloid β (Aβ) peptide Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA The binding activities of phage clones to the sRAGE were measured by phage ELISA. The OD values at 370 nm were measured and data shown were reproduced from the Fig. 1 in the reference. 2947 WTFNAGSHERTY(7/15)[1.24 ± 0.07] WTLQQEEQLVYY(1/15)[1.38 ± 0.12] WQLQSGEELRLT(1/15)[1.39 ± 0.11] AVEWGAGMPSYS(1/15)[1.04 ± 0.12] WGTYQWGTEAPN(1/15)[1.11 ± 0.10] WSLQAGENVTIF(1/15)[1.09 ± 0.18] NVPAPVALYQIS(1/15)[0.18 ± 0.04] PLYGDDANHYQW(1/15)[0.50 ± 0.36] SNWPTSRTYLSD(1/15)[0.29 ± 0.07] 9 Phage display (common panning) 3 PMID:26541335 Anti-plant potyviruses capsid proteins (CPs) monoclonal antibody C4 Capsid proteins (CPs) of plant potyviruses Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA The binding activities of the positive phage clones to the C4 MAb were measured by phage ELISA. The OD values at 405 nm were measured and data shown were reproduced from the Fig. 1 in the reference. 2948 LPLTPLP(6)[NT][NT] FHGAREM(4)[0.45 ± 0.01][0.36 ± 0.02] TTYSRFP(1)[NT][NT] NEISFHA(1)[0.62 ± 0.02][0.38 ± 0.03] LPLGHHE(1)[NT][NT] IGHLSFE(1)[NT][NT] GHLYDDP(1)[0.74 ± 0.02][0.64 ± 0.01] 7 Phage display (subtractive panning) 3 PMID:26543535 Maltase-glucoamylase (MGAM) Anti-MGAM polyclonal antibody Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA The binding activities of the positive phage clones to the maltase-glucoamylase were measured by phage ELISA and competitive phage ELISA. The A450 values were measured and data shown were reproduced from the Fig. 1a in the reference. In the competitive phage ELISA, a competitive inhibitor (0.5 mmol/L acarbose and 0.5 mmol/L voglibose) was added. M13 phage vector showing no peptide was used as control, and the corresponding affinity values were 0.17 ± 0.01 and 0.08 ± 0.01 respectively. NT means not test. In the second and third round of selection, an aliquot of amplified eluate from a previous round of biopanning was added to anti-MGAM antibody-coated beads. After 30 min of gentle shaking at room temperature, the supernatant was transferred to the enzyme-coated beads and was incubated for 60 min. 2949 CTHYGFRGC(2)[1.12 ± 0.01][0.79 ± 0.02] CGHHHRDYC(1)[1.07 ± 0.07][0.59 ± 0.02] 2 Phage display (subtractive panning) 3 PMID:26543535 Maltase-glucoamylase (MGAM) Anti-MGAM polyclonal antibody Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) ELISA The binding activities of the positive phage clones to the maltase-glucoamylase were measured by phage ELISA and competitive phage ELISA. The A450 values were measured and data shown were reproduced from the Fig. 1a in the reference. In the competitive phage ELISA, a competitive inhibitor (0.5 mmol/L acarbose and 0.5 mmol/L voglibose) was added. M13 phage vector showing no peptide was used as control, and the corresponding affinity values were 0.17 ± 0.01 and 0.08 ± 0.01 respectively. NT means not test. In the second and third round of selection, an aliquot of amplified eluate from a previous round of biopanning was added to anti-MGAM antibody-coated beads. After 30 min of gentle shaking at room temperature, the supernatant was transferred to the enzyme-coated beads and was incubated for 60 min. 2950 RDGSIAMHSMIP(1)[0.36 ± 0.01][0.27 ± 0.02] QALLEGNAKGGN(1)[NT][NT] GGTKTHVDFSLK(1)[NT][NT] 3 Phage display (subtractive panning) 3 PMID:26543535 Maltase-glucoamylase (MGAM) Anti-MGAM polyclonal antibody Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA The binding activities of the positive phage clones to the maltase-glucoamylase were measured by phage ELISA and competitive phage ELISA. The A450 values were measured and data shown were reproduced from the Fig. 1a in the reference. In the competitive phage ELISA, a competitive inhibitor (0.5 mmol/L acarbose and 0.5 mmol/L voglibose) was added. M13 phage vector showing no peptide was used as control, and the corresponding affinity values were 0.17 ± 0.01 and 0.08 ± 0.01 respectively. NT means not test. In the second and third round of selection, an aliquot of amplified eluate from a previous round of biopanning was added to anti-MGAM antibody-coated beads. After 30 min of gentle shaking at room temperature, the supernatant was transferred to the enzyme-coated beads and was incubated for 60 min.