2876 YHTTDKLFYMMR[1.78 ± 0.08] YSAYEFEYILSS[1.71 ± 0.05] KTMSAEEFDNWL[1.95 ± 0.11] LTSHTYRSQADT[0.68 ± 0.12] 4 Phage display (common panning) 4 PMID:26033236 Tocilizumab Interleukin-6 receptor Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Binding capabilities of the selected phages toward Tocilizumab was estimated by ELISA. The absorbance at 405 nm was measured and data shown were reproduced from the Fig. 1C in the reference. The experiments were performed in triplicate and presented as mean ± standard deviation. The mimotopes were conjugated to immunogenic carrier proteins and used to intraperitoneally immunize BALB/c mice. Sera from the mimotopes immunized mice not only showed specific binding to recombinant IL-6R, but can also IL-6R expressed in Hela, U-937, Jurkat cell lines and in fibroblast-like synoviocytes from patients with RA (FLS-RA). 2877 NKELQTV(7)[1.56 ± 0.2, 0.68 ± 0.18] VKELDTR(3)[1.78 ± 0.32, 1.22 ± 0.16] AKELSTW(2)[2.25 ± 0.23, 0.79 ± 0.32] K-E-L-x-T 3 Phage display (subtractive panning) 4 PMID:26048649 Imidacloprid hapten IMI Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA The binding activities of positive phage clones to IMI-BSA were measured by competitive phage ELISA respectively using 0 and 1000 ng/mL of imidacloprid. The absorbance at 450 nm was measured and data shown were reproduced from the Figure 2 in the reference. The conjugate IMI-BSA was used as binding-templates to select peptides specific for imidacloprid. Bound phages in the wells were eluted. The eluted phage solutions were transferred onto wells coated with 3% BSA alone to eliminate the non-specific binding phages. 2878 PHSNRKKKDTQR[23.95 ± 0.38] HYKYYPTASVMK[104.1 ± 0.76] FTKVKPKSHNMT[23.95 ± 3.02] WPLSTVASVRVP[39.52 ± 2.67] VPRSMAATHSTF[14.83 ± 2.64] PHSNRKKNQYLE[23.19 ± 5.3] QTWDLPRTTHKT[11.79 ± 2.28] 7 Phage display (competitive panning) 3 PMID:26052070 Site-specifically biotinylated Complement C3 beta chain (C3b) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Surface plasmon resonance (SPR) Direct binding of peptides was measured using SPR by injecting 1 mM of each peptide over site-specifically immobilized C3b. Data shown were reproduced from the Fig. 4B in the reference. A control peptide (YHPNGMNPYTKA) exhibits minimal response at this concentration, which had the Resonance Units (RU) value of 3.81 ± 0.76. Following washes of increasing stringency, bound phage were eluted using 100 μM MSCIN-A to specifically select for SCIN-competitive, C3b-binding sequences. Seven unique sequences exhibited direct C3b binding. A subset of these specifically inhibited the AP inassays of complement function. The mechanism of AP inhibition by these peptides was probed through surface plasmon resonance approaches, which revealed that six of the seven peptides disrupted C3bBb formation by interfering with factor B/C3b binding. 2879 KVWMPNNRGPVP AYTTSIPNRNQL TILKAWPNITQL AWPPPSMGPLAY KISQPTTVASLQ ILANDLTAPGPR TPFPFAPLGRPP KVWISPQSLGAT VSRHQSWHPHDL KCCTLPRPLEML NQRVLPPSHSWL LSLNFDRESETN KVWPPMYLSHTF AVPHRVGGLHSL NADNQMTWRHVL IDAPANQRLLQK KIWQMDQDSVRN HPHHETSVMRLQ KVWXMAPTTAFS SHPWNAQRELSV KVWQLQPSNSVT MKVSEPLHAHFS KAWVLTEWQTTK SIHRHHDDHFLT LLXKTLNNRLYD KVVQLSELSRLL DPALRHTHHNLR AHSHKLFLLSNR HSPSNLGFQSPP WMADTSPSLAST SWNSKAWIVVPA STMNDIHMELHR KCCFTTASSTSI GLKIWSLPPHHG KIXIFYPFKSPLI FPAMSFAXKKLA HFKPMQPAHPIP NSTLKVLPQGWM KVWPPLTSIVPS NLRTDSLTLVIR 40 Phage display (common panning) 3 PMID:26071378 Type IV secretion system protein virB10 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2880 DAQWAFM PPLSAPN GETPNPH LSSPTSN PLWDKRH NSWPPSR RLPHSVG QWTNHAL SGTPWHP DPFHVHN TTNSPPL FSSSRNY PATYTSA TQPWTRA NDFKLQF YKLPSRH ALGHSAK QKAHHEQ HEARSTM VIPQPQQ SYPTRPT FGNPHGH MRNDGAN PVPYPQL PKKLDSI KATAQGA HNPLLSF KPASHNA DSLQYPA TLPLPNL TNPVTES MSKHQAY PEAALSS TFLDTRA DPGASHP MERPAHL STHTSRL WAAIFPL PSKLQEH GPKMFRI LKITGED NRYTPTA LSYLHPT HPQLPSI NRGQLST GFLGKNQ KSAKTYH APSPWKQ PHTLSRT FSNQTML PAHRVPS CSTPTPH GMMPVAL 53 Phage display (common panning) 3 PMID:26071378 Type IV secretion system protein virB10 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2881 TQYYAYSTTQKS TQAIRVHTISSQ GLNQVLRIPSFI SPKHNLDMVKMM MIVDHLPIQVNT EYDYACGVVGYE SYPKASLALLAP 7 Phage display (common panning) 3 PMID:26058474 Magnesium fluoride (MgF2) particles Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2882 NQTHDWVPYAGR(8) YWPDERPFAARK(4) LHNPWRDTPPFA(4) TALDFPTWTAKN(4) VIDIPPFAYRNT(3) YADTPPFASTHK(2) STSDFLPYAARF(2) LPGVRDTPPFAA(2) VIGDMPPYAAMH(2) QWDLPPYAGRYD(2) GIDQLPWAARNK(2) YGIPWLDMPIKR(2) NQTDGQDRVPYA(1) TSPDAYPYAARQ(1) SDLPPFAAGRYN(1) NPFDTVPYAGRQ(1) RTLDWYDRVPYA(1) IQDWPPYASSTK(1) GERPPDYPPYAA(1) NHNQDFRPFPDR(1) KWQSDWPPFAAY(1) SDVDRPPWPART(1) SYVPDWPPFASK(1) APNVTDVPPFAA(1) IDTPPYAGRSSL(1) TLLIQRSPYEQI(1) SINPNPPLRPSY(1) D-x-[PVLP]-[YFW]-A-[AGS]-[RK] 27 Phage display (subtractive panning) 5 PMID:25520269 Anti-coagulation factor VIII monoclonal antibody (mAb) 2-76 Coagulation factor VIII Not determined. Not determined. Ph.D.-12 phage display library (X12) For selection of peptides that bind to the murine monoclonal anti-FVIII antibody (mAb) 2-76 immobilisation on streptavidin-coated beads was achieved via a biotin-conjugated rabbit anti-mouse IgG. For negative selections, an appropriate IgG isotype (murine IgG2a) was used. Three positive and two negative selections were performed. 2883 NPPKWPR(1) 1 Phage display (subtractive panning) 5 PMID:25520269 Anti-coagulation factor VIII monoclonal antibody (mAb) 2-76 Coagulation factor VIII Not determined. Not determined. Ph.D.-7 phage display library (X7) For selection of peptides that bind to the murine monoclonal anti-FVIII antibody (mAb) 2-76 immobilisation on streptavidin-coated beads was achieved via a biotin-conjugated rabbit anti-mouse IgG. For negative selections, an appropriate IgG isotype (murine IgG2a) was used. Three positive and two negative selections were performed. 2884 NFTHPGV(2) SSTVYGR(2) SKLDGEK(1) MHMYSPP(1) YSTEYTY(1) STHPLPP(1) NGTRYLG(1) WSRPPIM(1) LTLPRSF(1) FSAFPTS(1) LTSPLHI(1) RLPPQTP(1) WSTSYTM(1) DPKNQPA(1) LSAIPTR(1) 15 Phage display (subtractive panning) 5 PMID:25520269 Anti-coagulation factor VIII polyclonal antibody IgG from haemophilia A patient 1 Coagulation factor VIII Not determined. Not determined. Ph.D.-7 phage display library (X7) For positive selections, IgGs from FVIII inhibitor-positive plasma was immobilized on streptavidin-coated beads via a biotin-conjugated goat anti-human IgG. For negative selections, IgGs from a plasma pool of healthy individuals negative for FVIII-specific antibodies were immobilised likewise. 2885 NYTHPGS(1) WTHPGNR(1) NFTHPGS(1) WTHPGAQ(1) SFTHPGQ(1) YTHPGAA(1) T-H-P-G 6 Phage display (subtractive panning) 5 PMID:25520269 Anti-coagulation factor VIII polyclonal antibody IgG from haemophilia A patient 1 Coagulation factor VIII Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) For positive selections, IgGs from FVIII inhibitor-positive plasma was immobilized on streptavidin-coated beads via a biotin-conjugated goat anti-human IgG. For negative selections, IgGs from a plasma pool of healthy individuals negative for FVIII-specific antibodies were immobilised likewise. 2886 SNDFIHTWSTNW(3) DGNYTHTRSTNY(2) TPPYLHHFSTRY(2) QKSLHTFSTHYS(1) NCWTHPGATQCN(1) QGYNHVISTAYD(1) LTPITQLAGPMP(1) AIPYNHSVATYY(1) MGAHYRPQPNPH(1) LTYPDFLHNNFP(1) MYIPNNTPSMMR(1) LQVPWYATSTAK(1) FITPPSLPAFTG(1) ETQIHMVSTRYD(1) SMNYYHTFATNY(1) SPWLHTTSTHYL(1) STASNFTHPGND(1) SFTHRESTIYNA(1) DMIHTHYSSTRY(1) 19 Phage display (subtractive panning) 5 PMID:25520269 Anti-coagulation factor VIII polyclonal antibody IgG from haemophilia A patient 1 Coagulation factor VIII Not determined. Not determined. Ph.D.-12 phage display library (X12) For positive selections, IgGs from FVIII inhibitor-positive plasma was immobilized on streptavidin-coated beads via a biotin-conjugated goat anti-human IgG. For negative selections, IgGs from a plasma pool of healthy individuals negative for FVIII-specific antibodies were immobilised likewise. 2887 AMRVEPHTYKNH(7) AMLVDPQGAHSW(1) NPVEWFMSTVNT(1) GMKVEPWMPPPR(1) YMPVEPQSTYTK(1) AMPVESELYKLM(1) AMPVAPDTQRYR(1) SFNTRPLPFLSY(1) AQRVDPDLVSIS(1) [AG]-M-x-V-[ED]-P 9 Phage display (subtractive panning) 5 PMID:25520269 Anti-coagulation factor VIII polyclonal antibody IgG from haemophilia A patient 3 Coagulation factor VIII Not determined. Not determined. Ph.D.-12 phage display library (X12) For positive selections, IgGs from FVIII inhibitor-positive plasma was immobilized on streptavidin-coated beads via a biotin-conjugated goat anti-human IgG. For negative selections, IgGs from a plasma pool of healthy individuals negative for FVIII-specific antibodies were immobilised likewise. 2888 AMLVQPA(5) AMPVEPD(2) AMFVEPA(1) AMLVFPE(1) AMQVDPA(1) AMPVQPH(1) AMLVEPY(1) LTLQPTF(1) [AL]-M-x-[VQ]-E-P 8 Phage display (subtractive panning) 5 PMID:25520269 Anti-coagulation factor VIII polyclonal antibody IgG from haemophilia A patient 3 Coagulation factor VIII Not determined. Not determined. Ph.D.-7 phage display library (X7) For positive selections, IgGs from FVIII inhibitor-positive plasma was immobilized on streptavidin-coated beads via a biotin-conjugated goat anti-human IgG. For negative selections, IgGs from a plasma pool of healthy individuals negative for FVIII-specific antibodies were immobilised likewise. 2889 SLQPTEHNKFWL(2) MTTPTESHKFGH(1) DLLRPSEKNKFF(1) SYTPSELRVFQT(1) TPAPSERMHFDS(1) SPSELQKFQTRS(1) KSTPSEIRHFDY(1) [TS]-P-[ST]-E-x(2)-[KH]-F 7 Phage display (subtractive panning) 5 PMID:25520269 Anti-coagulation factor VIII polyclonal antibody IgG from haemophilia A patient 5 Coagulation factor VIII Not determined. Not determined. Ph.D.-12 phage display library (X12) For positive selections, IgGs from FVIII inhibitor-positive plasma was immobilized on streptavidin-coated beads via a biotin-conjugated goat anti-human IgG. For negative selections, IgGs from a plasma pool of healthy individuals negative for FVIII-specific antibodies were immobilised likewise. 2890 DAWIGSRIPFKG(14) LCLDISCHIPTK(2) SHSAIQDHMPRK(1) RTWHPSEIMHEY(1) VHMKQIPSWLIK(1) LDKQTLKYLHEK(1) VEGIAGHIPTKL(1) I-x(2)-[HR]-[ILM]-P-x-K 7 Phage display (subtractive panning) 5 PMID:25520269 Anti-coagulation factor VIII polyclonal antibody IgG from haemophilia A patient 10 Coagulation factor VIII Not determined. Not determined. Ph.D.-12 phage display library (X12) For positive selections, IgGs from FVIII inhibitor-positive plasma was immobilized on streptavidin-coated beads via a biotin-conjugated goat anti-human IgG. For negative selections, IgGs from a plasma pool of healthy individuals negative for FVIII-specific antibodies were immobilised likewise. 2891 GIIIPHQ[0.95 ± 0.04] MIPHDEN[1.16 ± 0.04] VHALVPH[0.85 ± 0.05] FSLIPHS[1.01 ± 0.09] ISLIPHT[1.03 ± 0.08] FVPHANK[0.95 ± 0.04] IMPLWPH[0.84 ± 0.04] LIPHDSL[1.19 ± 0.02] LNPNKLI[0.81 ± 0.05] WNLVPHT[0.88 ± 0.04] [IV]-P-H-D 10 Phage display (common panning) 3 PMID:25741007 Anti-avian hepatitis E virus (HEV) capsid protein monoclonal antibody 3E8 Capsid protein Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) ELISA Affinities of phages to mAb 3E8 were measured by phage ELISA. The OD values at 490 nm were measured and data shown were reproduced from the Fig. 2A in the reference. Error bars indicate the standard range of individual values from two independent experiments and the results were presented as mean ± standard deviation. 2892 KLAPHAN[1.07 ± 0.13] GLKLYQS[1.68 ± 0.06] FKLYMEP[1.63 ± 0.04] VKLYMTF[1.81 ± 0.04] VHALYLQN[0.96 ± 0.02] LDPARF[1.09 ± 0.15] GNLFMTL[1.43 ± 0.07] VKLFMSTL[1.73 ± 0.05] RDAVRPF[1.25 ± 0.03] V-K-L-Y-[MT]-S 9 Phage display (common panning) 3 PMID:25741007 Anti-avian hepatitis E virus (HEV) capsid protein monoclonal antibody 1B5 Capsid protein Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) ELISA Affinities of phages to mAb 1B5 were measured by phage ELISA. The OD values at 490 nm were measured and data shown were reproduced from the Fig. 2B in the reference. Error bars indicate the standard range of individual values from two independent experiments and the results were presented as mean ± standard deviation. 2893 RATQLPQ(4/12) QHIPKPP(2/12) HRRPSRS(1/12) AFDTHTM(1/12) TPPEPAP(1/12) NQDVPLF(1/12) HRLRISP(1/12) GDTQRVA(1/12) 8 Phage display (common panning) 4 PMID:25947144 δ2 CDR3 peptide CACDVLGVNTDKLIFGKG Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Four peptides that bound to the predominant δ2 CDR3 fragments and showed homology to M.tb genes in a BLAST search. Notably, one fragment was related to M.tb H37Rv (QHIPKPP), and this fragment was confirmed as a ligand for the γδ TCR. 2894 SEISAST(2/10) ATKTRQP(2/10) LNPVHRL(1/10) YPTGLPL(1/10) RFRPTPP(1/10) ADHPPPH(1/10) SRVRLGA(1/10) HRSHSTH(1/10) 8 Phage display (common panning) 4 PMID:25947144 δ2 CDR3 peptide CACDTLGDRDYTDKLIFGKG Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2895 GWHHHPR(5/12) HKRPRNN(1/12) HRRPSRS(1/12) RRRPMAI(1/12) HGSTKRT(1/12) WNPHKHH(1/12) WRQTRKD(1/12) GTTTTLL(1/12) 8 Phage display (common panning) 4 PMID:25947144 δ2 CDR3 peptide CACDTIGTGGHYTDKLIFGKG Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2896 SPRVGAT(2/13) ATKTRQP(1/13) HKRPRNN(1/13) AFDTHTM(1/13) SEISAST(1/13) QHIPKPP(1/13) RRAPTPP(1/13) YMQLPSH(1/13) KLAPMTS(1/13) GWHHHPR(1/13) ASPDTPQ(1/13) 11 Phage display (common panning) 4 PMID:25947144 δ2 CDR3 peptide CACDTLWGIQGNTDKLIFGKG Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2897 TDFNTMAKNNPP[4.97] 1 Phage display (common panning) 3 PMID:26040578 Biotinylated anti-lysozyme polyclonal antibody IgG Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Binding capabilities of the selected phage toward biotinylated anti-lysozyme polyclonal antibody IgG was estimated by ELISA. The absorbances at 418 nm were measured and data shown were reproduced from the Fig. 4 in the reference. The affinity value was 4.97 when the biotinylated anti-lysozyme polyclonal antibody IgG was at a concentration of 3e-8. 2898 STGTPRA(3) KSTSQPE(2) TRTPQHS(1) LSTRAPL(1) DRAPGRT(1) LSPARTT(1) PPYLSTR(1) TTTPTLH(1) TMTGSTT(1) PYSAKAH(1) PPYLSHL(1) RPAPNQT(1) PHPISKQ(1) TNTAWTS(1) MTSSGML(1) HHTTSTG(1) ISQPIRQ(1) 17 Phage display (common panning) 4 PMID:25802296 Biopolymer transport protein ExbD (43–141) Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2899 FSISTLQSAPTR(2) QTTAWWGAPARL(2) QMMQTSSSPPTV(1) STNPAALYSDYS(1) HHRAHNSHLSVR(1) HSIFYPIYLPSQ(1) FHESWPSPAGGR(1) FPHYPVSTLYSL(1) STPQPSLFSYPV(1) FHSSTPTAPPQK(1) NGLTSSRPWSFL(1) SPIYVTWVPTAL(1) NDPGRLRVPVST(1) RHYEPLSRVSSS(1) STPQVYNVFYAP(1) FHSHWPSMADNS(1) TVYWITPPALPI(1) FHETWPARVSYL(1) SHHWEPISSPLR(1) KIYPITLTYLAP(1) SSKVLPSSFFTR(1) APTTQTPPINWK(1) QTNSQHPISALR(1) RHSEPISVFYIT(1) HPIYVTYYPDPS(1) HLLMKPPQTSPA(1) 26 Phage display (common panning) 3 PMID:25802296 Biopolymer transport protein ExbD (43–141) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2900 SHSNTTQTRPSD SHALPLTWSTAA NTIPMHTSTHTI IHPASQSRQNTT AALGTYSTHTPT HLPTSSLFDTTH HGLPVTTRGAFG TKTVAQTTTSIS KLVDESSTSPLS HSNLPTKRPTSL 10 Phage display (common panning) 3 PMID:25802296 Protein TonB (33-239) Ferrienterobactin receptor Not determined. Not determined. Ph.D.-12 phage display library (X12)