2826 WCFDVCRVGCL(2) VCFPLCRVPCI(2) GCYKPCRVNCP(2) DCFGRCRVRCL(2) NCFPLCRVGCE(1) GCFQECRVVCV(1) SCFQTCRVYCL(1) QCFERCRVNCD(1) DCFHQCRVGCD(1) KCWGLCRVGCL(1) VCWSPCRVACS(1) ACYEACRVNCV(1) SCYTGCRVYCI(1) TCDLVCQVRCR(1) RCPMSCNYRCV(1) KCGKKCDYRCL(1) TCEKRCCPRCS(1) 17 Phage display (subtractive panning) 2 PMID:23100545 Plasma kallikrein (EC:3.4.21.34) Not determined. Not determined. Not determined. XCX3CX3CX phage display library The library was joined and subjected to a first affinity selection with hPK. Isolated phage were amplified and, prior to the second round of affinity selection, treated for 30 min with 5 mg/ml pancreatin. The second round of panning was performed using neutravidin-coated magnetic beads to prevent the enrichment of streptavidin-specific peptides. 2827 ECFQGFCWRDTCW(2) SCFSGFCWRQMCS(1) SCFQAACWRQLCG(1) RCERALCVWWWCD(1) SCGRDGCLWKSCR(1) 5 Phage display (subtractive panning) 2 PMID:23100545 Plasma kallikrein (EC:3.4.21.34) Not determined. Not determined. Not determined. XCX4CX4CX phage display library The library was joined and subjected to a first affinity selection with hPK. Isolated phage were amplified and, prior to the second round of affinity selection, treated for 30 min with 5 mg/ml pancreatin. The second round of panning was performed using neutravidin-coated magnetic beads to prevent the enrichment of streptavidin-specific peptides. 2828 GCWPQCRVNCV(3)[115.7 ± 24] TCEKRCCPRCS(3)[NT] KCWQECRSLCY(2)[NT] GCYERCRVYCS(2)[NT] GCYKPCRVNCP(1)[158.7 ± 25.7] ECFTACRVACI(1)[NT] ECFDKCRVFCV(1)[NT] GCFTPCRTMCW(1)[NT] TCFHHCRVNCS(1)[NT] ACWKLCRVDCV(1)[NT] NCWARCRVMCE(1)[NT] FCFQNCKALCF(1)[NT] SCVRPCFYWCD(1)[NT] SCVDYCVGTCC(1)[NT] LCMDNCEQVCQ(1)[NT] KCGKKCDYRCL(1)[NT] 16 Phage display (subtractive panning) 2 PMID:23100545 Plasma kallikrein (EC:3.4.21.34) Not determined. Not determined. Not determined. XCX3CX3CX phage display library Inhibition assay Bicyclic peptides were chemically synthesized and their inhibitory activity (IC50, nM) is indicated. NT denotes not tested. The library was joined and subjected to a first affinity selection with hPK. Isolated phage were amplified and, prior to the second round of affinity selection, treated for 30 min with 10 mg/ml pancreatin. The second round of panning was performed using neutravidin-coated magnetic beads to prevent the enrichment of streptavidin-specific peptides. 2829 SCFQAACWRQLCG(2) NCRAKGCWYGYCF(1) ACRSWSCTCGLCY(1) SCGGDQCALDRCV(1) TCSWGKCQVTDCG(1) 5 Phage display (subtractive panning) 2 PMID:23100545 Plasma kallikrein (EC:3.4.21.34) Not determined. Not determined. Not determined. XCX4CX4CX phage display library The library was joined and subjected to a first affinity selection with hPK. Isolated phage were amplified and, prior to the second round of affinity selection, treated for 30 min with 10 mg/ml pancreatin. The second round of panning was performed using neutravidin-coated magnetic beads to prevent the enrichment of streptavidin-specific peptides. 2830 ACFDPCRVVCY(1)[18.2 ± 4.2] QCFQWCRTNCY(1)[178.3 ± 12.6] NCWNPCRVACL(1)[51.7 ± 10.4] QCENPCPTSCC(1)[NT] TCEKRCCPRCS(4)[NT] 5 Phage display (subtractive panning) 2 PMID:23100545 Plasma kallikrein (EC:3.4.21.34) Not determined. Not determined. Not determined. XCX3CX3CX phage display library Inhibition assay Bicyclic peptides were chemically synthesized and their inhibitory activity (IC50, nM) is indicated. NT denotes not tested. The library was joined and subjected to a first affinity selection with hPK. Isolated phage were amplified and, prior to the second round of affinity selection, treated for 30 min with 20 mg/ml pancreatin. The second round of panning was performed using neutravidin-coated magnetic beads to prevent the enrichment of streptavidin-specific peptides. 2831 TCERRACVMRECA(1) GCVQGKCVLATCT(1) 2 Phage display (subtractive panning) 2 PMID:23100545 Plasma kallikrein (EC:3.4.21.34) Not determined. Not determined. Not determined. XCX4CX4CX phage display library The library was joined and subjected to a first affinity selection with hPK. Isolated phage were amplified and, prior to the second round of affinity selection, treated for 30 min with 20 mg/ml pancreatin. The second round of panning was performed using neutravidin-coated magnetic beads to prevent the enrichment of streptavidin-specific peptides. 2832 CSTYNPYCQLKTSSC(7)[>75] CAQDEKSCWYTPLKC(4)[>75] CTAQNPYCALKQRSC(3)[NT] CTQGPGGCKDHLLLC(3)[NT] CPADQMLCKGLFCLC(3)[NT] CLSMERKCVKTQLCC(3)[NT] CSSLDASCWFSQTKC(2)[>75] CSRNGQDCFWSSIKC(2)[NT] CNRDTENCYWTQVKC(2)[NT] CLQNNYLCFPGTKPC(2)[NT] CAGANPYCLAKGQAC(2)[>75] CLGTPRKCTKTELCC(2)[NT] CTVKPSFCGNGGQAC(2)[NT] CSSFAQDCWFSYTKC(1)[NT] CTESPQTCWYTATKC(1)[NT] CFADTTECWFSITKC(1)[NT] CNSVNDMCWYSQTKC(1)[NT] CQGPSYDCFYSVSKC(1)[NT] CAQTDGGCYWSSVKC(1)[NT] CARDEQGCFYSVVKC(1)[>75] CQKKDEQCFWSAVKC(1)[>75] CSQNAPGCFYSKVKC(1)[NT] CVQGQADCFFSSAKC(1)[NT] CEDSWKTCWYSQVKC(1)[NT] CPGDVDSCFYSSVKC(1)[NT] CQPDEEGCWWNQVKC(1)[NT] CTIGHDECWYQTTKC(1)[NT] CGLATPDCWWNQTKC(1)[NT] CPTQGGECWQTQVKC(1)[NT] CYNAKETCWDSLTRC(1)[NT] CNTDQNLCYYSKSGC(1)[NT] CWWTSTKCRQEDNNC(1)[NT] CWQTVTKCGQSFVGC(1)[NT] CWVTQAKCPQASTEC(1)[NT] CWTTQVKCGGGAGDC(1)[NT] CDNINPYCVVKEQKC(1)[NT] CSEANPYCWLKPNQC(1)[NT] CNLIQPYCQLKDHMC(1)[NT] CPSLQPYCGVKWALC(1)[NT] CLLSSPYCAVKGQGC(1)[NT] CELRVPYCVVKNQRC(1)[NT] CIRLNPYCSQKFTMC(1)[NT] CSLNEKYCTQVKKPC(1)[NT] CEVPAVKCQFWGRGC(1)[NT] CQVPLAKCNWFLWPC(1)[>75] CQSWSRGCAKEILCC(1)[NT] CKIMNAYCAKTSLCC(1)[NT] CTNKQLFCPEKRQCC(1)[NT] CQDMNNICGSKAKFC(1)[NT] CQDWVGECFFTKIGC(1)[NT] CQAGNTACMFPAKHC(1)[NT] CQHAAEGCWHTIIKC(1)[NT] CQGKASFCEQLGHVC(1)[NT] CQGVMTLCFPQTKLC(1)[NT] CLQTADPCLKTPVKC(1)[NT] CLQNTEPCVYFATKC(1)[NT] CLQGVTVCGVKGTIC(1)[NT] CREQISKCFDGQEWC(1)[NT] CAEDLWGCIQLKSKC(1)[NT] CGQYTKFCFHNWNGC(1)[NT] CYKSLGTCQGRLSWC(1)[NT] [WF]-[FYW]-[ST]-x-[TV]-K 61 Phage display (subtractive panning) 3 PMID:23586812 Human alpha-FXIIa Not determined. Not determined. Not determined. CX6CX6C phage display library Inhibition assay Several bicyclic peptides presenting common sequence elements were chemically synthesized and their inhibitory activity (Ki, μM) is indicated. All indicated inhibitory constants Kis are averages of at least three measurements. NT denotes not tested. In the second round, neutravidin-coated magnetic beads were used to prevent the enrichment of streptavidin-specific peptides. 2833 GCVSDKCIIQYCN(5)[42 ± 4] ACVTGKCLLQLCP(3)[>75] ECVVDKCLVQFCP(3)[NT] QCVEDKCIQSLCA(3)[>75] NCVSQKCRWEYCP(3)[NT] DCVKPRCPQGGCR(3)[NT] LCKTQGCVSFSCE(3)[NT] YCVMDKCLQTVCT(2)[NT] HCFKAKCPIQGCW(2)[>75] TCVQGKCLITNCG(1)[NT] ECVQGKCLWSMCL(1)[49 ± 8] MCVQGKCLYLYCQ(1)[NT] GCVQGKCLQELCA(1)[NT] SCVQGKCLVSMCR(1)[NT] DCVVGKCLSQVCS(1)[NT] DCVVGKCLYDFCK(1)[NT] SCVVGKCLVQYCA(1)[NT] GCVIGKCLTYVCE(1)[NT] GCVSGKCLQIFCQ(1)[NT] DCVEGKCLQVNCL(1)[NT] SCVEGKCLAQFCS(1)[NT] GCVKGKCLQEFCP(1)[NT] LCVQDKCLPTRCP(1)[NT] FCVVEKCLAQQCQ(1)[NT] YCVREKCLPYNCY(1)[NT] SCVWDKCLQAICH(1)[NT] ACVAQKCLWDFCR(1)[NT] VCVMQKCLWNVCE(1)[NT] NCVQQKCLWSACL(1)[NT] ECVPVKCLWQTCA(1)[NT] QCVPSKCLYAMCN(1)[NT] ECVVGKCVWENCL(1)[NT] YCVSGKCRQEVCS(1)[NT] QCVFDKCIQNLCI(1)[NT] QCVFDKCIQNLCI(1)[NT] YCVLDKCQPLRCP(1)[NT] GCVPQHCGGPRCF(1)[NT] ACVQSLCNSVNCY(1)[NT] HCWLAKCPVQGCN(1)[NT] PCPIQLCGSSKCR(1)[NT] QCPLQLCGWVKCG(1)[NT] LCPDALCGSAKCM(1)[NT] LCPQEFCGSLKCG(1)[>75] DCQFSWCAVKRCP(1)[NT] GCQAFWCAVKQCY(1)[>75] GCQGSRCVKTLCC(1)[NT] WCQNGGCVKTLCC(1)[NT] GCQARACAKFLCC(1)[NT] GCGKKLCAKELCC(1)[NT] TCSEKRCSKLLCC(1)[NT] WCTHQRCVKARCC(1)[NT] RCKVAYCAKDLCC(1)[NT] RCGQGFCSYTKCS(1)[NT] SCSAGFCSPVKCP(1)[NT] GCWWGLCRYKECP(1)[NT] QCFSPYCMVKGCP(1)[NT] SCDYPWCQVKMCP(1)[NT] VCSPLKCGSAVCR(1)[NT] QCPKPRCQLVLCP(1)[NT] GCQNWACPVKPCH(1)[NT] K-C-[LI], AVK 60 Phage display (subtractive panning) 3 PMID:23586812 Human alpha-FXIIa Not determined. Not determined. Not determined. XCX4CX4CX phage display library Inhibition assay Several bicyclic peptides presenting common sequence elements were chemically synthesized and their inhibitory activity (Ki, μM) is indicated. All indicated inhibitory constants Kis are averages of at least three measurements. NT denotes not tested. In the second round, neutravidin-coated magnetic beads were used to prevent the enrichment of streptavidin-specific peptides. 2834 CSRTQDECWWTLTKC(42) CMQRTVACVKQTLCC(5) CTESPQTCWYTATKC(4) CSRNGQDCFWSSIKC(4) CSQGDLKCWWTLTKC(3) CDRVRPYCQVKGAAC(3) CFKGSSDCFYSQVKC(2) CNRDTENCYWTQVKC(2) CGMVNKRCVKELLCC(2) CIRYSFLCGAGGGEC(2) CMDEVWMCFGITGLC(2) CGISSRSCWYTITKC(1) CEGTDSQCWYSIVKC(1) CQGMVDTCFWSALKC(1) CDQSMQACFWSSIKC(1) CQGPSYDCFYSVSKC(1) CYKGPVSCFWNPTKC(1) CQKGPVSCFWNPTKC(1) CQGGQENCWLNPTKC(1) CQDGGYSCFFTKTGC(1) CQLVYPYCRVKVENC(1) CVYKMPYCYVKQLNC(1) CSTYNPYCQLKTSSC(1) CMYRAPYCQIKQTSC(1) CEAVNPYCQRKGRSC(1) CPADQMLCKGLFCLC(1) CEWHQKICKGTFCLC(1) CQEMKWWCPSVKYHC(1) CSQVHGWCPSAKYGC(1) CTKGQRSCAKLSLCC(1) CDAFSRGCVKQHMCC(1) CMSRMATCVKASRCC(1) CGFMRHSCSKTTLCC(1) CGGFRWCCDISKIRC(1) CSGQRWCCDVRSARC(1) CSSSRWCCSYQSVLC(1) CLRTLGSCGSTMLAC(1) CYHENAYCKRKWGDC(1) CSGKWLYCGQSAKLC(1) CLKCAGACPFKGQAC(1) CRQDWWVCEWSWSTC(1) CVSPRQMCTWPKSNC(1) CDKGTRKCGYLRFEC(1) [WF]-[WY]-[TS]-x-[TVL]-K, PYCQ 43 Phage display (subtractive panning) 3 PMID:23586812 Human beta-FXIIa Not determined. Not determined. Not determined. CX6CX6C phage display library In the second round, neutravidin-coated magnetic beads were used to prevent the enrichment of streptavidin-specific peptides. 2835 ACDARPCPQTYCL(28)[20.5 ± 5.2] SCGGRPCPPAYCK(22)[3.1 ± 0.5] GCLGRPCPMAYCS(13)[5.0 ± 1.5] DCPVVWCLQKACR(7)[46.9 ± 3.2] VCVQKFCWRGWCP(4)[>75] QCNARPCPSSYCR(2)[4.7 ± 1.5] GCMGRPCPVSYCF(2)[5.0 ± 1.3] ACQQQFCWRGWCP(2)[NT] LCEYTLCWRGWCP(2)[NT] HCRYVFCWRGWCP(2)[NT] WCVMQKCLMQYCE(2)[NT] YCVKDKCLQAMCS(2)[NT] SCGGPRCWPSTCR(1)[NT] RCGQGFCSPVKCR(1)[NT] ICKSQVCVSFSCG(1)[NT] GCWARPCPLALCQ(1)[10.2 ± 4.6] GCAARPCPLTACW(1)[33.5 ± 5.9] GCHGRPCPLQYCK(1)[11.2 ± 4.4] RCYANPCPISYCR(1)[NT] SCSGRRCPPSYCK(1)[7.8 ± 3.2] NCVNRYCWRGWCS(1)[NT] SCVVGKCLVQYCA(1)[NT] HCVQGKCLVYMCG(1)[NT] QCVPFKCLLHLCA(1)[NT] GCVLGKCLQDFCQ(1)[NT] YCVPLKCLWDRCE(1)[NT] RCSAGGCCERVCE(1)[NT] TCTTQGCAFRLCS(1)[>75] WCTHQRCVKARCC(1)[NT] YCQGTKCVKERCC(1)[NT] LCQPRKCVKALCC(1)[NT] SCANQRCVKSLCC(1)[NT] TCLCKRCIKELCC(1)[NT] VCTYRKCVKELCC(1)[NT] GCQARACAKFLCC(1)[NT] GCGRNICVKNLCC(1)[NT] [AG]-R-P-C-P, WRGW, V-K-x-[RL] 36 Phage display (subtractive panning) 3 PMID:23586812 Human beta-FXIIa Not determined. Not determined. Not determined. XCX4CX4CX phage display library Inhibition assay Several bicyclic peptides presenting common sequence elements were chemically synthesized and their inhibitory activity (Ki, μM) is indicated. All indicated inhibitory constants Kis are averages of at least three measurements. NT denotes not tested. In the second round, neutravidin-coated magnetic beads were used to prevent the enrichment of streptavidin-specific peptides. 2836 CDSRFRNCPWSMSGC[1500] CSDRFRNCPLWSGTC[400] CRDIRFRCNYDVAVC[NT] CSTERRYCPIEIFPC[400] CAPWRTMCLNIDGPC[NT] CAPWRTACYEDLMWC[500] CPVWRTLCMESEGVC[NT] CFPLWRTCVHEPTMC[NT] CWQVQVNCRVNFGKC[800] CGGNSDRCRVNNISC[2000] CGRGDQTCRVNWHPC[NT] CGTGEGRCRVNWTPC[500] APWRT, RFRN, RVN 12 Phage display (subtractive panning) 3 PMID:19483697 Plasma kallikrein (EC:3.4.21.34) Not determined. Not determined. Not determined. CX6CX6C phage display library Inhibition assay The IC50 (nM) was measured by incubating various concentrations of the modified peptide fusion proteins with human plasma kallikrein (0.1 nM) or cathepsin G (20 nM). In the second round of selection, neutravidin-coated magnetic beads were used to prevent the enrichment of streptavidin-specific peptides. 2837 CEYGDLWCGWDPPVC[NT] CEYDVGFCWDGFGDC[100] CLFDAGFCQQHSTEC[NT] CIFDLGFCHNDWWNC[100] CLFDLGFCGGGEGPC[150] CPRIEGFCLPIFSDC[1000] CLRAQEDCVYDRGFC[200] CTRGSGDCTYDFGFC[200] LFD 8 Phage display (subtractive panning) 3 PMID:19483697 Cathepsin G (EC:3.4.21.20) Not determined. Not determined. Not determined. CX6CX6C phage display library Inhibition assay The IC50 (nM) was measured by incubating various concentrations of the modified peptide fusion proteins with human plasma kallikrein (0.1 nM) or cathepsin G (20 nM). In the second round of selection, neutravidin-coated magnetic beads were used to prevent the enrichment of streptavidin-specific peptides. 2838 CNERFSMCGQMGLRC(5)[NT] CNAKFSGCRYSALVC(2)[3.66 ± 0.09] CNSRFSGCQIDLLMC(2)[NT] CNAKFSGCVGRGGHC(1)[NT] CNAKFSLCGNSVFGC(1)[NT] CNAYFSGCNSLTGGC(1)[NT] CNAYFSGCDWAVQHC(1)[2.638 ± 0.081] CNARFSGCQPSPAAC(1)[NT] CNNKFTLCGSRQIIC(1)[NT] CNWKFSLCETQRNQC(1)[NT] CNSKFTGCRAGTGLC(1)[11.136 ± 0.294] CNSKFTQCGAPRGTC(1)[NT] CNSRFALCSPSSQMC(1)[NT] CNSKYSGCQRSPASC(1)[NT] CNSKYSGCGGQRYDC(1)[NT] CNSRYSLCTGAILTC(1)[12.341 ± 0.331] CNERYALCGILGFQC(1)[NT] CSRYEVDCRGRGSAC(1)[0.071 ± 0.004] CAEQVIDCRGRGGLC(1)[NT] N-[AS]-[KRY]-[FY]-[ST], DCRGRG 19 Phage display (subtractive panning) 2 PMID:22304751 Urokinase-type plasminogen activator (EC:3.4.21.73) Not determined. Not determined. Not determined. CX6CX6C phage display library Competition experiment The inhibitory activity of bicyclic peptides was determined by incubating human uPA with different concentrations of inhibitor and quantification of the residual activity with a fluorogenic substrate. The inhibitory activities (Ki, μM) of five TBMB-cyclized peptides (as D1-D2 fusion peptides) are indicated. A second round of panning was performed following the same procedure but using neutravidin-coated magnetic beads in order to prevent the enrichment of streptavidin-specific peptides. 2839 AAKTPTE(8) ATTVPAS(4) MSLQQEH(4) SAPSSKN(2) LPRTPTD(2) MHAPPFY(1) DEQPYDL(1) TSLSMPS(1) 8 Phage display (common panning) 3 PMID:25663979 Visfatin Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2840 ICARQDPAGNCS[1.54] KCPSIPGAVLCV[1.57] 2 Phage display (common panning) 3 PMID:25710003 Polyclonal anti-LiPA IgG L. infantum chagasi Protein Antigen (LiPA) Not determined. Not determined. LX-8 phage display library (XCX8CX) ELISA Individual clones isolated was added to each well of microtiter plates coated with 1 μg/well of anti-LiPA IgG and anti- T. cruzi IgGs. Phages were detected using a peroxidase conjugated anti-M13 antibody (1 : 3000). Values of absorbance at 492 nm are measured. The A492 values of phages with ICARQDPAGNCS and KCPSIPGAVLCV insert for anti- T. cruzi IgGs were 0.19 and 0.19, respectively. Data shown were reproduced from graph. Anti-LiPA IgGs were coated onto a polystyrene Petri dish overnight at a concentration of 5 μg/mL for the first two rounds of panning and a concentration of 0.5 μg/mL for the last panning. 2841 TTDDDKLKKTLTYRS[1.51] 1 Phage display (common panning) 3 PMID:25710003 Polyclonal anti-LiPA IgG L. infantum chagasi Protein Antigen (LiPA) Not determined. Not determined. X15 phage display library ELISA Individual clones isolated was added to each well of microtiter plates coated with 1 μg/well of anti-LiPA IgG and anti- T. cruzi IgGs. Phages were detected using a peroxidase conjugated anti-M13 antibody (1 : 3000). Values of absorbance at 492 nm are measured. The A492 value of phages with TTDDDKLKKTLTYRS insert for anti- T. cruzi IgGs was 0.18. Data shown were reproduced from graph. Anti-LiPA IgGs were coated onto a polystyrene Petri dish overnight at a concentration of 5 μg/mL for the first two rounds of panning and a concentration of 0.5 μg/mL for the last panning. 2842 CFKACRVNC[5.2 ± 1.8] CFKQCRVNC[NT] CFKNCRVNC[NT] CFYKCRVNC[NT] CFNKCRVNC[NT] CFPKCRVAC[NT] CFKHCRVAC[5.2 ± 1.9] CFDPCRVIC[NT] RVN 8 Phage display (subtractive panning) 3 PMID:22492508 Plasma kallikrein (EC:3.4.21.34) Not determined. Not determined. Not determined. CX3CX3C phage display library Competition experiment Target specificity of bicyclic peptides was indicated by the Ki values (nM) of these peptides against hPK, monkeyPK and rPK. The Ki values of one peptide CFKACRVNC against monkeyPK and rPK were 2.8 ± 1.7 and 22.7 ± 14.0, and for the other peptide CFKHCRVAC, the corresponding values were 4.4 ± 1.1 and 14.0 ± 8.2. In the second round of selection, neutravidin-coated magnetic beads were used to prevent the enrichment of streptavidin-specific peptides. 2843 SCFNRCRVPCF[NT] KCFTACRVDCF[NT] RCFNLCRVGCL[NT] DCFQGCRVFCS[NT] QCFELCRVVCL[NT] MCFDSCRVNCT[NT] SCFALCRVPCQ[NT] VCFPLCRVPCI[37.1 ± 8.1] VCFGVCRVLCA[NT] KCFQACRVSCY[NT] VCFSLCRAGCL[NT] RCFEKCRAFCY[NT] RCWQTCRVSCV[NT] LCWTGCRVSCF[NT] GCWAPCRVGCY[NT] RCWTACRGLCF[NT] DCYQLCRVSCE[NT] TCFRSCKVACY[NT] QCFQACKTLCW[NT] 19 Phage display (subtractive panning) 3 PMID:22492508 Plasma kallikrein (EC:3.4.21.34) Not determined. Not determined. Not determined. XCX3CX3CX phage display library Competition experiment Target specificity of bicyclic peptides was indicated by the Ki values (nM) of these peptides against hPK, monkeyPK and rPK. The Ki values of the peptide VCFPLCRVPCI against monkeyPK and rPK were 12.2 ± 7.4 and 59.7 ± 22.4 respectively. In the second round of selection, neutravidin-coated magnetic beads were used to prevent the enrichment of streptavidin-specific peptides. 2844 CAWPARCLTVDLC[0.4 ± 0.1] CRWPARCVHQDLC[0.3] CRWPARCLTTSLC[NT] CAWPAKCLTRELC[NT] CAWPARCLTTSLC[0.9 ± 0.1] CSWPARCNHQDLC[NT] CRWPARCTHQNYC[2.9 ± 1.2] CTWPARCTHQNWC[NT] CTWPARCTMQNYC[NT] CSWPYRCLHQDYC[NT] CNWPYRCLHTDLC[NT] CGVPYRCTHQEMC[NT] CTYPYKCLHQNLC[12.2 ± 4.8] CADPWACLFRRPC[NT] CGGPQNCRTWTTC[4.3 ± 0.2] CGGYNNCRAFSYC[NT] CFPSHDCDGRRMC[5.3 ± 2.8] PAR 17 Phage display (subtractive panning) 3 PMID:22492508 Plasma kallikrein (EC:3.4.21.34) Not determined. Not determined. Not determined. CX5CX5C phage display library Inhibition assay Target specificity of bicyclic peptides was indicated by the Ki values (nM) of these peptides against hPK. In the second round of selection, neutravidin-coated magnetic beads were used to prevent the enrichment of streptavidin-specific peptides. 2845 IGLTRY[0.36] DGSSKL[0.32] TLVSSL[0.30] LPAKSP[0.27] ALGRFQ[0.26] SIFPPK[0.26] LASNRQ[0.21] FRLIRS[0.20] LDAMVS[0.19] PWAWTS[0.19] WYTAPT[0.17] MVKWGT[0.15] SLWRLS[0.15] PLGVMR[0.14] HGSTEV[0.13] KFAGVN[0.13] HPTQST[0.12] AEKVLH[0.11] NNGGLT[0.09] SSRIGF[0.08] AWALRF[0.07] HPTGSM[0.06] 22 Phage display (subtractive panning) 7 PMID:16760366 Phosphatidylserine, PS Not determined. Not determined. Not determined. X6 fUSE5 phage dislpay library ELISA Phages bound to PS-coated plates were detected by incubation with horseradish peroxidase (HRP)–conjugated anti-M13 phage antibody followed by addition of the o-phenylenediamine dihydrochloride (OPD) substrate. Accumulation of the reaction product was detected by absorbance measured at 450 nm (A450 nm). Data shown were reproduced from graph. Four and three consecutive panning rounds of the phage library were performed, respectively, on control livers and on apoptotic livers. Four selected phages (IGLTRY, DGSSKL, TLVSSL, LPAKSP) were further evaluated for binding to PS-coated enzyme-linked immunosorbent assay (ELISA) plates. They presented an apparent affinity constant (Ka) for PS ranging from 6.08e10 M to 1.62e11 M. These phages did not bind to phosphatidylcholine, and competition with annexin V confirmed their specific interaction with PS. 2846 DAHSFS(5) DAHSLS(1) PGDLST(7) PGDLSR(3) PGDLVR(1) HGDLST(1) HGHLSI(1) VLGERG(1) LIKKPF(1) 9 Phage display (subtractive panning) 4 PMID:19743879 Phosphatidylserine, PS Not determined. Not determined. Not determined. X6 fUSE5 phage dislpay library The libary was incubated (2 h, room temperature) with a preblocked well (in the absence of PS) to remove phages that interact with plastic surface. And then, libary was transferred to the PS coated well and incubated overnight at room temperature under mechanical agitation. 2847 ARLHTSS(7) SMTKIMC(5) NGPFGKC(1) LDYKHYC(1) DYHLKPC(1) AACVQTC(1) KIPRVFC(1) MSVSKPC(1) AQHRTHL(1) 9 Phage display (common panning) 3 PMID:25469678 Anti-buffalo β-lactoglobulin antibody from rabbit 1 Beta-lactoglobulin, Beta-LG Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2848 SMTKIMC(3) IPLVVPF(2) AACVQTC(2) KIPAVFC(2) SEHGRNC(1) WMEQNGC(1) SVKPVRC(1) GIDWLYC(1) GIDWLYC(1) TQRGPAC(1) MTSVMMC(1) DYHLKPC(1) 12 Phage display (common panning) 3 PMID:25469678 Anti-buffalo β-lactoglobulin antibody from rabbit 2 Beta-lactoglobulin, Beta-LG Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2849 WHTGTPH(11) LHKHPTP(3) VHPHWRH(1) WHSGTPH(1) 4 Phage display (subtractive panning, competitive panning) 4 PMID:25565805 Mycobacterium tuberculosis (H37Rv) Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) In the first round of biopanning, inactive MTB reference strain H37Rv was used as the target. For three further rounds of biopanning, Mycobacterium smegmatis was used as the reverse screening molecule with increased stringency of the washing steps. After four rounds of screening, Clones were eluted by H37Rv. 2850 WHTGTPH(4) VHPHWRH(4) LHKHPTP(3) CHPHTPH(1) 4 Phage display (subtractive panning, competitive panning) 4 PMID:25565805 Mycobacterium tuberculosis (H37Rv) Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) In the first round of biopanning, inactive MTB reference strain H37Rv was used as the target. For three further rounds of biopanning, Mycobacterium smegmatis was used as the reverse screening molecule with increased stringency of the washing steps. After four rounds of screening, Clones were eluted by Mycobacterium smegmatis.