251 HLPTSSLFDTTH(1) NPHWSSLYAPRN(1) HTQNMRMYEPWF(1) GQSPHSYQPRTY(1) TPSVLSTALHSS(1) SLTHAWQQTHFL(1) WSVTNLVLLSPP(1) FAKNSNSRILDQ(1) YQLRPNAESLRF(1) SNWYNGLEFLET(1) 10 Phage display (competitive panning) 3 PMID:16517013 UDP-N-acetylmuramoylalanine--D-glutamate ligase ATP Not determined. Not determined. Ph.D.-12 phage display library (X12) Phage encoding peptides were eluted in the each round of biopanning using glycine-HCl, glycine-HCl and ATP. 252 CIPSHTPRC(5) CGPMGISTC(1) CSSMPLPAC(1) CSSSPMRTC(1) CRDTLFSQC(1) CLRSAGPSC(1) 6 Phage display (competitive panning) 3 PMID:16517013 UDP-N-acetylmuramoylalanine--D-glutamate ligase D-glutamate Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Phage encoding peptides were eluted in the each round of biopanning using glycine-HCl, glycine-HCl and D-Glu. 253 HLPTSSLFDTTH(3) LNRTPSLPSVHA(1) LQGSFSIHGNPP(1) TAGKVTASLIGR(1) VLGTKWPPMPLS(1) DHASTWMVKRGV(1) SSLPTPSESPSR(1) 7 Phage display (competitive panning) 3 PMID:16517013 UDP-N-acetylmuramoylalanine--D-glutamate ligase D-glutamate Not determined. Not determined. Ph.D.-12 phage display library (X12) Phage encoding peptides were eluted in the each round of biopanning using glycine-HCl, glycine-HCl and D-Glu. 254 CKGTINPFC(45) CNVHRGLHC(16) CKLTANPTC(2) CXGAINPFC(1) 4 Phage display (common panning) 7 PMID:16882546 Trisialogangliosides (GT1b), NGF-differentiated PC12 cells Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) In the present study, we have designed a biopanning strategy using two tiers of selection: the first on GT1b, and the second on NGF-differentiated PC12 cells. We have also combined different strategies to recover bound phage. In the first tier of biopanning, acidic elution (Glycine-HCl-pH 2.2) was used to capture all GT1b bound phage, then rTTC elution was used to select for phage with binding at the clostridial toxin receptor. In the second tier of cellular biopanning, PC12 cell lysis was used to recover all phage binding to or taken up by the neuronal cell line. Phage was amplified and titered after each round. 255 GRRTRSRRLRRS(7) GRRIAGPYIALE(5) TPRNLRTSNTHR(4) SMPINSPYIPWS(4) SMSIASPQIPWS(3) GRRINRLILPRN(3) GRRTRSSRLRNS(2) GRRPMKLNKTP(2) 8 Phage display (subtractive panning) 4 PMID:16458253 XGC9811-L cell line Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) High liver-metastatic cell variant XGC9811-L was chosen as a tester and its parental cells XGC9811 cell as a depletory. With a subtraction/selection protocol, multirounds of selection were carried out in our experiment. Before each round of selection, XGC9811 cells were used for preincubation with the library in order to remove the non-specific phages. Specific phages binding to liver-metastatic XGC9811-L cells were obtained from the phage peptide library and further enriched round by round. 256 CGRWSGWPADLC[56] 1 Phage display (common panning) 4 PMID:16252253 Integrin alpha-X-beta-2, integrin αxβ2 Intercellular adhesion molecule 1, ICAM-1 Not determined. Not determined. CL10 phage display library (CX10C) Surface plasmon resonance (SPR) The bonding phage was eluted with low pH. 257 CHKGHDRGKKRC(5) 1 Phage display (common panning) PMID:16252253 Integrin alpha-X-beta-2, integrin αxβ2 Intercellular adhesion molecule 1, ICAM-1 Not determined. Not determined. CL10 phage display library (CX10C) The bonding phage was eluted with EDTA-containing buffer. 258 MDKTHFVNE 1 Phage display (common panning) PMID:16252253 Integrin alpha-M-beta-2, integrin αmβ2 Intercellular adhesion molecule 1, ICAM-1 Not determined. Not determined. LL9 phage display library (X9) The bonding phage was eluted with low pH. 259 CPGGEWRSKAKC 1 Phage display (common panning) PMID:16252253 Integrin alpha-M-beta-2, integrin αmβ2 Intercellular adhesion molecule 1, ICAM-1 Not determined. Not determined. CL10 phage display library (CX10C) Bound phage were eluted with anti-CD11b/CD18 antibody CBRM1/29. 260 AHKSARKTE WSYWETVAK 2 Phage display (common panning) PMID:16252253 Integrin alpha-M-beta-2, integrin αmβ2 Intercellular adhesion molecule 1, ICAM-1 Not determined. Not determined. LL9 phage display library (X9) Bound phage were eluted with EDTA-containing buffer. 261 SSQVVGVPQLMQSSP(1) SAYAATVRGPLSSAS(1) DRVPLVHVIFNSFGY(1) RNQGPVKMVFPIAPS(1) EGQFTFPRGASE(1) 5 Phage display (common panning) 3 PMID:16546989 DNA topoisomerase 1 Not determined. Not determined. Not determined. f3-15mer phage display library (X15) 262 AWPLSQLDHSYN YHLSSQQLDHSL ATWGHPRSSQGM QLDSH 3 Phage display (common panning) 3 PMID:16938891 Anthrax toxin receptor 1 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 263 CPSSTLFAC 1 Phage display (common panning) 3 PMID:16938891 Anthrax toxin receptor 1 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 264 SPHGSTDHSTTA 1 Phage display (common panning) 3 PMID:16938891 Anthrax toxin receptor 2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 265 STDHSLY STDSGWV 2 Phage display (common panning) 3 PMID:16938891 Anthrax toxin receptor 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 266 CTSTDATYC 1 Phage display (common panning) 3 PMID:16938891 Anthrax toxin receptor 2 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 267 KSLSRHDHIHHH(5) 1 Phage display (common panning) 3 PMID:16953561 Hepatoma cell line SMMC-7721 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The KD (μM) was calculated and shown. 268 CLVDAAALC(8) CPIALGLKC(3) CGGPLKGLC(2) CINLGLTMC(1) CFSLGLIKC(1) CPAYKLYSC(1) CGSRSKGTC(1) CNSVGGRSC(1) 8 Phage display (common panning) 6 PMID:15306709 Plasmodium falciparum-infected red blood cells (iRBCs) Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library 269 KQRTSIRATEGCLPS(5) GRHRTSVPTDEVFIT(1) KKSHHPSSEWGLNLT(1) KQRDSRSGYTAPTLV(1) RNHGTDRATTIPPLS(1) 5 Phage display (subtractive panning) 3 PMID:15980377 Nontypeable Haemophilus influenzae, strain R2866 Not determined. Not determined. Not determined. f88-15mer phage display library (X15) To isolate peptides binding to strain-specific epitopes, we chose to preadsorb the starting library against a nonvirulent NTHi (Rd KW20) prior to affinity selecting for peptides binding to R2866. 270 PSALGRFTRGPL SLIFVTISSEWG LSLSLDLLTFRT PDIRHYFIQNRG GCRIVYRRPLHL RTAGFDIKLIDT RIQYQAISTVSL 7 Bacterial display (common panning) 5 PMID:15895468 Anti-sperm polyclonal antibody Sperm Not determined. Not determined. FliTrx bacterial display library (X12) 271 CEVSHPKVGC(4) CRARGQGWC(3) CRPYTGWKEC(2) CVGVGGTIPC(2) CIRGVARDSC(2) CEPEIRSNNC(2) CRVCRTWVLC(2) CWVTTSNQWC(1) CSGGSNRSPC(1) CKTIPSAATC(1) CTE*RKRRIC(1) 11 Phage display (common panning) 3 PMID:16397382 Newcastle disease virus Not determined. Not determined. Not determined. pSKAN8-HyA phage display library 272 TSNYSILYTEFA WDLTYELDRLWT ANLINEFDDLAS ESRLTSEFDGIH 4 Phage display (common panning) 3 PMID:16183141 Anti-NS4A monoclonal antibody 2E3C2 Non-structural protein 4A, NS4A Not determined. Not determined. Ph.D.-12 phage display library (X12) 273 TVLTNPSTNHLS(2) AANMNMSRVSHT(2) TACHQHVRMVRP(1) STAELHFPMVFP(1) ASPPSYALPVTP(1) APHHTNITEIRI(1) 6 Phage display (subtractive panning) 3 PMID:16598852 Hepatocellular carcinoma cell lines BEL-7402 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) After the first round of panning, the phages were treated with normal liver cell line HL-7702 in the same way for reverse absorption, and the unbound phages were collected to eliminate the phages which can bind to liver cells. The residual phages were amplified and tittered for next round panning. After three rounds of panning and two rounds of reverse absorption the peptide sequences of randomly picked phage clones were analyzed by DNA sequencing. 274 NGNNVNGNRNNN 1 Phage display (subtractive panning) 4 PMID:16455333 Monoclonal antibody CM22 IgM Antigens exposed in ischemic tissue Not determined. Not determined. Ph.D.-12 phage display library (X12) The library was biopanned 4 times with the pathogenic CM22 IgM clone and then negatively selected against the inactive CM31 IgM clone. 275 GPGVSSAPPFSK LKSSGSAPPGPF AGKASKIPDPGF VPLSAGAPPLMA 4 Phage display (common panning) 3 PMID:16934463 Silver nanowires Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The generated binding peptide sequences indicate that both serine and proline residues are important for binding to AgNW, and the obtained sequences bear strong resemblance to the sequences previously obtained against AgNP. Taken together, these observations suggest that amino acid residues may be binding to the Ag atoms, and is indiscriminate of their structural morphologies.