2701 LLHLRL[2.89] LALFLA[3.74] LRQVWL[3.5] PLALLH[3.14] QLGLNP[3.89] TNFMLP[3.69] TWLGQG[3.56] SPLTSM[2.53] VSDVVW[3.73] 9 Phage display (common panning) 4 PMID:21666888 PDZ domain of segment polarity protein dishevelled homolog DVL-2 Not determined. Not determined. Not determined. X6 M13 phage display library ELISA ELISA signal/control ratio is shown. The phages were propagated in the absence of IPTG to obtain medium peptide display level and allow the selection of a wider range of interacting sequences. 2702 CWGHSRDEC(15) CSGGRRLEC(4) G-[HR]-[RS]-R-[DL]-E 2 Phage display (common panning) 4 PMID:16239579 Aspergillus conidium Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library Screening of the phage library was performed by the BRASIL method. 2703 CLLSATPSC(11) ATPS 1 Phage display (common panning) 4 PMID:16239579 Aspergillus conidium Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library Screening of the phage library was performed by the BRASIL method. 2704 CGGVRGLDC(6) CSGGRRLEC(4) CTGGRVLSC(1) G-G-R-[GR]-L-[DE] 3 Phage display (common panning) 4 PMID:16239579 Aspergillus conidium Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library Screening of the phage library was performed by the BRASIL method. 2705 CGARASGSC(5) CARADAATC(2) G-A-R-A-[DS]-[AG]-[AS] 2 Phage display (common panning) 4 PMID:16239579 Aspergillus conidium Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library Screening of the phage library was performed by the BRASIL method. 2706 ATRDGSS(4) SGSSIDQ(1) GSSSGNF(1) GSS 3 Phage display (common panning) 4 PMID:16239579 Aspergillus conidium Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library Screening of the phage library was performed by the BRASIL method. 2707 DANRVGG(1) GAIRSGL(1) GWQGFGS(1) SGEVGRG(1) GGWGPGS(1) AGSGLSN(1) AGGQKSI(1) TGGRVLS(1) VGVRPDS(1) GWHPGQE(1) XXHAGQA(1) EGLRRSA(1) EYLGLAR(1) A-x-R-x-G, G-x(2)-G-x-G, A-G-x(3)-S, G-x-R-x(2)-S, H-x-G-Q, G-L-x-R 13 Phage display (common panning) 4 PMID:16239579 Aspergillus conidium Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library Screening of the phage library was performed by the BRASIL method. 2708 CRESVRDRC(1) CNEYSRPGC(1) CXAGPXRTC(1) CTVDSARSC(1) CDGRGMAC(1) CXTRSGHXC(1) CLEWTGLDC(1) CATGTHGPC(1) 8 Phage display (common panning) 4 PMID:16239579 Aspergillus conidium Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library Screening of the phage library was performed by the BRASIL method. 2709 CGPIVSFGC(1) CAGTGVSGC(1) CGGSKVSAC(1) CRNGSHVSC(1) CGAAVSILC(1) CGAVVSVDC(1) G-[AST]-x-V-S 6 Phage display (common panning) 5 PMID:16239579 Aspergillus hypha Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library Screening of the phage library was performed by the BRASIL method. 2710 CGGRLGPFC(2) CGDRLPHFC(1) CRSIGRLGC(1) CPAEGSLGC(1) [GD]-[RS]-L-G 4 Phage display (common panning) 5 PMID:16239579 Aspergillus hypha Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library Screening of the phage library was performed by the BRASIL method. 2711 CVLLRSSGC(1) CFLLRSNDC(1) CRLWRSTGC(1) CVPLSRSTC(1) L-x-R-S-x 4 Phage display (common panning) 5 PMID:16239579 Aspergillus hypha Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library Screening of the phage library was performed by the BRASIL method. 2712 RALAHPRDHPDL[1.25] ATCSMLLSRNEA[1.11] 2 Phage display (subtractive panning) 3 PMID:24779651 Esophageal cancer cell line Eca109 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Phage ELISA was used to test the specific binding of the selected phage clones to Eca109 cells. Normal human esophageal epithelial cells and bovine serum albumin cells served as controls. Absorbance values at 490 nm were subsequently recorded using a plate reader. The data were reproduced from the graph. Three rounds of subtractive screening of a phage peptide library were performed using Eca109 as the target cells and normal human esophageal epithelial cells as the absorber cells. For the second and third rounds of selection, the volume of added phage remained the same, yet the incubation time with the Eca109 cells decreased to 45 min and 30 min, respectively. In contrast, the incubation time with the normal esophageal cells increased to 1.25 h and 1.5 h, respectively. While ELISA, immunofluorescence and immunohistochemistry assays were used to validate the binding affinities of two positive phage clones, sequencing of the positive clones did not find any homology between the sequences obtained and a protein database. 2713 CKSLENSYC(18)[23.4 ± 1.1] CKSVGNYQC(2)[3.6 ± 0.8] CKNPTTGTC(2)[4.3 ± 0.3] CKSLENSQC(1)[NT] CNPSNRNDC(1)[NT] CKSPGNYQC(1)[NT] CKSLEQSYC(1)[NT] CNPSNRQDC(1)[NT] CTGTTNQYC(1)[NT] CNSPRPSTC(1)[NT] CNVQHNKTC(1)[NT] CSTRHTYDC(1)[NT] CIKSTHNHC(1)[NT] 13 Phage display (subtractive panning) 6 PMID:24841092 Bisphenol A, BPA Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Binding assay The binding specificity and relative affinity of the selected peptides to BPA were compared by assessing the number of BPA bound phages with varying peptide sequences displayed. Wild type M13 phage was used as a control. The number of wild type phages bound to BPA was 2.3 ± 0.2. NT denotes not tested. Two rounds of negative panning followed the six rounds of positive screening procedure. The collected phages from the final round of positive panning were incubated in a microcentrifuge tube filled with Binding Buffer lacking BPA for 1 h and centrifuged as above in order to exclude phage particles with cross binding affinity to the tube surface. 2714 CSMSARQLC(20/25) 1 Phage display (common panning) 4 PMID:24960578 Beta-amyloid protein 42 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Twenty-five Aβ42-binding clones with higher absorbance values were selected and amplified. Twenty clones showed the same peptide sequence: CSMSARQLC. His-tag-conjugated peptide CSMSARQLC bound to Aβ42 in a concentration-dependent manner. The peptide recognized Aβ13–35. In addition, molecular dynamics analysis and ELISA showed that the peptide predominately binds to I31 of Aβ42 by leucine and may also bind to F20 by alanine. 2715 DSRLEPNT ASRAPSST GGSVPTET DRATSSNA 4 Phage display (common panning) 3 PMID:24977927 Envelope glycoprotein E2 Not determined. Not determined. Not determined. f8-8mer phage display library (X8) Four phage clones specifically binding the E2 protein of CSFV were screened from f8/8 landscape phage library, and the phage E2P4 displaying octapeptide DRATSSNA significantly inhibited the CSFV infection in PK-15 cells at a higher titer. 2716 GVIMVIAVSCVF(11)[0.952 ± 0.244] GSLVMLVFGYMG(4)[0.947 ± 0.239] 2 Phage display (subtractive panning) 3 PMID:24979051 Sera IgG from patients with PMI Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA The original phage library as a negative control. The absorbance value at 450 nm was measured. In the first round of affinity selection, microtiter wells were coated with purified sera IgG from patients without PMI to absorb nonspecific phages from the phage peptide library, and the unbounded phages were then incubated with sera IgG from patients with PMI to screen for phages containing potential peptide biomarkers. Another two rounds of affinity selection were carried out in the same way. Among the 17 positive phage clones, 11 had the same peptide sequence GVIMVIAVSCVF and four had the same peptide sequence GSLVMLVFGYMG. 2717 CSPLTENKC(7) CTALNENKC(1) CSQKSSLMC(1) CSSSSVGTC(1) CFPKKPNAC(1) CPKSTQPQC(1) CQYMDRSQC(1) CNRAFVTDC(1) CPHRGNEDC(1) 9 Phage display (common panning) 3 PMID:24689774 Anti-buffalo β-lactoglobulin antibody from rabbit 1 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2718 CGALEENKC(1) CSPYQENRC(1) CPLNENKDC(1) CPTNENREC(1) CVAGQSPKC(1) CTHAPLANC(1) CNALSENKC(1) CNPGSENKC(1) CSPLLENRC(1) CPFPRSAAC(1) CPLHENKSC(1) CGPQSENRC(1) CPYSENRAC(1) CSKTPPLTC(1) CPFDENKSC(1) CSALAENKC(1) 16 Phage display (common panning) 3 PMID:24689774 Anti-buffalo β-lactoglobulin antibody from rabbit 2 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2719 QAWTALT SHLNSWL YRAPWPP QGSTTPN TQWASYE SVTTKLN HTTSTTP TPRFLNY GSLYNLH YLRPPSA TVPGPSG TLVGDIV GMRWSEF NPAIISI VPPYWMS 15 Phage display (competitive panning) 4 PMID:24819195 β2-glycoprotein I, β2GPI Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Ultimately phage clones were obtained in elution step with polyclonal HAv anti-β2GPI antibody. 2720 ARLQLWL QPWPTST RADDLWL FQASLLP IAAHNPL AALTHIW AFSPLTI SGVAPRL RIGPELH 9 Phage display (competitive panning) 4 PMID:24819195 β2-glycoprotein I, β2GPI Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Ultimately phage clones were obtained in elution step with monoclonal chimeric IgG anti-β2GPI antibody (HCAL). 2721 HYYPWLP SHATGPW TTAPLLP MTTPSLH FVLPANY GLLASRS VSLAGIA FMMPHHQ VRHINIF WSRPATL LSWMPVV VNSSNYS 12 Phage display (competitive panning) 4 PMID:24819195 β2-glycoprotein I, β2GPI Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Ultimately phage clones were obtained in elution step with polyclonal LAv anti-β2GPI antibody. 2722 SAYHPWP MGTQLSW QLSTSAH IPKTPYS NPKIVLT LSKYPSA VTSYPFF TGSFPAL 8 Phage display (common panning) 4 PMID:24819195 β2-glycoprotein I, β2GPI Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2723 CKYMSVWKC CHSMSSHQC 2 Phage display (common panning) 4 PMID:24819195 β2-glycoprotein I, β2GPI Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2724 SLDSDRS(3/50) 1 Phage display (common panning) 3 PMID:24819195 Monoclonal chimeric IgG anti-β2GPI antibody, HCAL Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Fifty phage clones were randomly picked. Three phage clones exhibited significantly higher binding to HCAL than to background in preliminary ELISA and were identical in their primary structure. 2725 KMDGNHP(1/20) 1 Phage display (common panning) 3 PMID:24819195 Low avidity (LAv) IgG anti-β2GPI polyclonal antibody from patient B Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) From each selection experiment, 20 phage clones were randomly picked and tested for their affinity towards corresponding polyclonal anti-b2GPI. Only one phage clone with displayed peptide KMDGNHP significantly and selectively bound to polyclonal LAv anti-β2GPI fraction.