2676 CVWDWGDC(11) CVWDLGRC(2) CVWDQGIC(2) 3 Phage display (subtractive panning) 3 PMID:9261123 Coxsackievirus A9, CAV9 Not determined. Not determined. Not determined. CX5C+CX6C+CX7C+CX9 phage display library pool Prior to panning with the virus, the phages were incubated for 1 h at 4°C in BSA-coated wells in 500 ml of Tris-buffered saline supplemented with 1 mg/ml BSA and 0.5 mM MgCl2. 2677 (D/N)TPVCYMNWCVE(S/T)D N(N/T)KRCYMDLCIQTP ANTWCYVDECMRIA ETYGCFMDWCKLVT 4 Phage display (common panning) 3 PMID:11298226 Anti-aflatoxins B1 and G1 monoclonal antibody 24, MAb 24 Not determined. Not determined. Not determined. f88-Cys4 phage display library (X4CX4CX5) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2678 (E/M)GTICP(M/T)DIKGCN(H/Q)TP GCEQCYVDYCYCSDAG GCEQCYVDYCYCSDAG MRGQCYMNQNMCKHPA 4 Phage display (common panning) 3 PMID:11298226 Anti-aflatoxins B1 and G1 monoclonal antibody 24, MAb 24 Not determined. Not determined. Not determined. f88-Cys6 phage display library (X4CX6CX4) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2679 FHPRCNEMTCHIKP NNPTCPWLTCPLPS NTNHCYMDHCIQTH VANGCEKPWCNTTR TRW(N/D)C(P/R)TTYCPPSG 5 Phage display (common panning) 3 PMID:11298226 Anti-aflatoxins B1, G1 and B2 monoclonal antibody, MAb 13 Not determined. Not determined. Not determined. f88-Cys4 phage display library (X4CX4CX5) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2680 RQSYCHPWEAICHQHK RQSYCHPWEAICHQHK VDLWCPPAPWQCLPSD 3 Phage display (common panning) 3 PMID:11298226 Anti-aflatoxins B1, G1 and B2 monoclonal antibody, MAb 13 Not determined. Not determined. Not determined. f88-Cys6 phage display library (X4CX6CX4) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2681 AIGTAFL(6) INNALPT(4) LPNNALP(1) NNNALPT(1) NPDVTRS(1) NPDDRKA(1) LRTTAKL(1) LLTTAKP(1) MRTTSKT(1) LSLVPPA(1) LLTTNKD(1) 11 Phage display (subtractive panning) 3 PMID:17236253 MH01 plasma Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2682 TNCVGKDRCVTL(9) HACTGKLRCTTT(6) HKYPCSTTNCSP(6) NWGYSGMLAKIA(5) SHFPPWSLAWTH(4) MCQGKNICTTIQ(3) EQVQSPPWAPAW(1) HSVKLPPWSSVW(1) YPPWNLTWLSTP(1) KTAIGQLSSTLL(1) KAPLSTLSGSLL(1) VTPTRILSSSFT(1) KGFQPSLPLWPR(1) EGPSPLNNATLT(1) TLNSNTTKPPLA(1) GSLIQHTQVPWE(1) KLVMHTAVPYHI(1) TCITKTADLTRR(1) TDITKTADLTRR(1) QNNALPYPVSPL(1) TATPDLTLYMPG(1) 21 Phage display (subtractive panning) 3 PMID:17236253 MH01 plasma Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2683 CLGPGRAFC(18) CNLNTSKEC(3) CNNNTSKAC(1) CNNNASPDC(1) CNPDDRKAC(1) 5 Phage display (subtractive panning) 3 PMID:17236253 MH01 plasma Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2684 VPWETVW(4) VPWNAAW(2) LRTTAKL(1) LLTTAKP(1) MRTTSKT(1) LSLVPPA(1) LLTTNKD(1) AVPIWAR(1) 8 Phage display (subtractive panning) 3 PMID:17236253 MH04 plasma Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2685 TNCVGKDRCVTL(9) HACTGKLRCTTT(6) HKYPCSTTNCSP(6) SHFPPWSLAWTH(4) EQVQSPPWAPAW(1) HSVKLPPWSSVW(1) YPPWNLTWLSTP(1) KTAIGQLSSTLL(1) KAPLSTLSGSLL(1) VTPTRILSSSFT(1) KGFQPSLPLWPR(1) AKTITTGPLSSF(1) TLNSNTTKPPLA(1) ELPPWSSVWRTP(1) YKQPIDGGRLLF(1) TATPDLTLYMPG(1) 16 Phage display (subtractive panning) 3 PMID:17236253 MH04 plasma Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2686 CLGPGRAFC(18) CAWAPPWEPAWC(8) CNTNTGKTC(4) CNLNTSKEC(3) CNNNTSKAC(1) 5 Phage display (subtractive panning) 3 PMID:17236253 MH04 plasma Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2687 SKTIMAGPELRL(1) TLNSNTTKPPLA(1) LSPQFLSPTHWP(1) TATPDLTLYMPG(1) 4 Phage display (subtractive panning) 3 PMID:17236253 MH03 plasma Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2688 SVLSVWEIPGKL(2) ASAKWSIGPGRA(1) GIIWDHSSLPTL(1) 3 Phage display (subtractive panning) 3 PMID:17236253 MH06 plasma Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2689 KSLHYGPYRTSL(3) RLIEKTTTPWGP(1) 2 Phage display (subtractive panning) 3 PMID:17236253 MH07 plasma Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2690 LLSTPWY(1) 1 Phage display (subtractive panning) 3 PMID:17236253 MH05 plasma Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2691 TLNSNTTKPPLA(1) TATPDLTLYMPG(1) 2 Phage display (subtractive panning) 3 PMID:17236253 MH05 plasma Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2692 CDSPCVACC(1) 1 Phage display (subtractive panning) 3 PMID:17236253 MH05 plasma Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2693 STSPTHR(1) 1 Phage display (subtractive panning) 3 PMID:17236253 MH02 plasma Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2694 FCAGALTCTTLP(8) TLNSNTTKPPLA(1) TATPDLTLYMPG(1) 3 Phage display (subtractive panning) 3 PMID:17236253 MH02 plasma Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2695 CACSGRLTC(8) 1 Phage display (subtractive panning) 3 PMID:17236253 MH02 plasma Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection. 2696 LTFEHYWAQLTS(4) QQMHLMSYAPGP(1) TIRPSTTMDSPT(1) YANPQMEKAFES(1) LPNLTWALMPGA(1) YANPQMEKAFAS(1) LLADTTHHRPWT(1) 7 Phage display (common panning) 4 PMID:17875722 p53-binding domains of MDM2 Cellular tumor antigen p53 1YCR,4HFZ, Not determined. Ph.D.-12 phage display library (X12) 2697 LTFEHYWAQLTS(7) FAPLNRTVETSP(1) YAVSSSPRVAAL(1) VVHVPNSATPPR(1) 4 Phage display (common panning) 4 PMID:17875722 p53-binding domains of MDMX Cellular tumor antigen p53 3DAB, Not determined. Ph.D.-12 phage display library (X12) 2698 LCSPLV(4) LVYMVL(3) 2 Phage display (common panning) 5 PMID:18542831 PDZ domain of rho guanine nucleotide exchange factor 11 Not determined. Not determined. Not determined. X6 M13 phage display library The panning experiment was carried out with the library packed in the absence of IPTG. 2699 FAPFAL(1) VYGWLV(1) VARVYW(1) MRSVHV(1) LWSFSK(1) LLTYRP(1) HQESRM(1) LRSSPW(1) GPVILA(1) MEARLD(1) YVCRFA(1) YALRRA(1) LSVLDA(1) SVVLPR(1) 14 Phage display (common panning) 5 PMID:18542831 PDZ domain of rho guanine nucleotide exchange factor 11 Not determined. Not determined. Not determined. X6 M13 phage display library The panning experiment was carried out with the library packed in the presence of IPTG, which increased the number of fusion P8 protein copies on each phage particle as a consequence of Lac induction. 2700 LFFMRL(1) VQSSQI(1) TISLAL(1) RFGLRS(1) GFTHQS(1) NGCKST(1) GASTVS(1) YYSWSV(1) YGGGSF(1) GSGSHF(1) APPQYH(1) MDPTRP(1) CPRSLQ(1) 13 Phage display (common panning) 4 PMID:18542831 PDZ domain of rho guanine nucleotide exchange factor 12 Not determined. Not determined. Not determined. X6 M13 phage display library The panning experiment was carried out with the library packed in the presence of IPTG, which increased the number of fusion P8 protein copies on each phage particle as a consequence of Lac induction.