2651 GLHSRCHIGRDCSSA(9/21) GFVCSGIFAVGVGRC(7/21) HYGDCRYDLGSCRGA(2/21) APGVRLGCAVLGRYC(1/21) ACVMYDFVLRGMCAR(1/21) 5 Phage display (common panning) 3 PMID:9528007 Anti-CTLA4 monoclonal antibody Not determined. Not determined. Not determined. X15 fUSE5 phage display library 2652 CKGGRAKDC CVMGSVTGC CRGEVLWSC 3 Phage display (subtractive panning, in vivo) 4 PMID:15133506 White fat Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) In each biopanning round, an adult obese leptin-deficient [Lep(ob/ob)] female mouse was injected intravenously through the tail vein with 1.0e10 transducing units (TU) of the library. Phage (～300 TU/g in round 1 increased to ～e4 TU/g in round 3) were recovered after 5 min of circulation from subcutaneous fat and bulk-amplified for each subsequent round. Phage amplified after the third round of panning were enriched for fat-specific binders by adapting an in vivo subtraction step: a lean, C57BL/6 female was injected intravenously with 1.0e9 TU of phage selected in round 3. After 5 min, the unbound phage were recovered from circulation and amplified for the fourth and final round of biopanning in a Lep(ob/ob) female mouse. 2653 CYPLRCDYSSAAPC 1 Phage display (competitive panning) 2 PMID:9387141 Angiogenin Not determined. Not determined. Not determined. CX12C phage display library The phages bound specifically to the actin-binding site of angiogenin were competitively eluted with bovine actin. 2654 CRGDGWMC(10) CRGDGWC(8) CRGDGLMC(7) CRGDGWLC(5) CRGDGMLC(5) CRGDGFC(3) CRGDGLMCGL(2) CRGDGMLC(1) CRGDGWIC(1) CRGDGWWC(1) CRGDGLIC(1) CRGDGLDC(1) CRGDGLLC(1) CRGDGLWC(1) CRGDGFLC(1) CRGDGQHC(1) CRGDGAFC(1) CRGDGAWC(1) CRGDNVWC(1) CRGDNAWC(1) CRGDAAWC(1) CRGDAAHC(1) CRGDRAWC(1) CPVRRGDGWC(1) CDWRGDNQFC(1) CLRGDGLALC(1) CWRGDHVMPC(1) CGQRGDGFCL(1) CRGDGYCVFF(1) CRGDCLPTPR(1) CYVNGRAWAC(1) CTNVNGRSAC(1) CQGMHGTPAC(1) CQGIDGTPAC(1) CGQGMHRLAC(1) CMWLSVNYSC(1) CREQPASRSC(1) CKWRSARDLC(1) CVDCILRYLC(1) CGADSEEGPC(1) RGD 40 Phage display (common panning) 2/3/4 PMID:9634769 Integrin alpha-5-beta-1, integrin α5β1 Fibronectin, FN Not determined. Not determined. CX5C+CX6C+CX9 FUSE5-based phage display library pool 2655 CWRGDTPC(1) CWRGDRAC(1) CLRGDRVC(1) CSRGDGRC(1) CRGDSLRC(1) CRGDGRNC(1) CTTRGDSFC(1) CRVRGDSWC(1) CLRRGDSGC(1) CISRGDTFC(1) CPSRGDALC(1) CAGRGDALC(1) CSPRGDAGC(1) CTRRGDATC(1) CVRRGDAFC(1) CLSRGDVVC(1) CNARGDGFC(1) CVTRGDHFC(1) CEVRGDRIC(1) CNIRGDKIC(1) CNARGDKLC(1) CPRGDSTLC(1) CTRGDSIFC(1) CTRGDSLDC(1) CGRGDSHHC(1) CDRGDSQSC(1) CSRGDTYLC(1) CLRGDIANC(1) CGRGDLIHC(1) CSRGDGAIC(1) CFRGDDRKC(1) CRGDSFVGC(1) CRGDSHLQC(1) CRGDNTFGC(1) CRGDTVYAC(1) CRGDHGTLC(1) CRGDAWPGC(1) CRGDLAWVC(1) CRGDGIRFC(1) CRGDKGWNC(1) CARRLDAPC(1) CPSRLDSPC(1) CKTPGRLDC(1) CTTRSDSFC(1) CWSISPYFC(1) CPDLLAQSC(1) CLVLPSTGC(1) CYSLGFLVC(1) CQARGDRPRC(1) CNRRGDNWGC(1) RGD 50 Phage display (common panning) 2/3/4 PMID:9634769 Integrin alpha-V-beta-3, integrin αvβ3 Fibronectin, FN Not determined. Not determined. CX5C+CX6C+CX7C+CX9 phage display library pool 2656 CRRGDFGGC(2) CYARGDYPC(2) CSGRHDDYC(2) CFSRGDFPC(1) CHIRGDFPC(1) CRYRGDLPC(1) CSARGDWPC(1) CKRGDWIRC(1) CGARGDSRC(1) CRRMDMPDC(1) CWARRDMPC(1) CWVRSDLGC(1) CPLRRDWIC(1) CTARSDRRC(1) CMSRADRPC(1) CHSTRDELC(1) CRGDNYWC(1) CRGDNSAC(1) CPRGDWPC(1) CGRGDQLC(1) CVRGDRMC(1) CRGDRALC(1) CRGDTRSC(1) CGRGDGDC(1) CRQDVPQC(1) CRADVPLC(1) CGRLDVPC(1) CYRRDVPC(1) CKGDMPRC(1) CRHDSPRC(1) CKRRDYPC(1) CTRTDGWC(1) CMRTDGRC(1) CRTRDSPC(1) CVVRDMPC(1) RGD 35 Phage display (common panning) 2/3/4 PMID:9634769 Integrin alpha-M-beta-3, integrin αmβ3 Fibrinogen alpha, beta and gamma chain 2VDO,2VDP,2VDQ,2VDR, Not determined. CX5C+CX6C+CX7C FUSE5 phage display library pool 2657 CVTRGDNFC(2) CDCRGDCFC(2) CDCRGDCLC(2) CIRRGDTFGC(2) CAPRGDHFAC(2) CRGDTFC(1) CRGDVFLC(1) CWTRGDSFC(1) CEGRGDSFC(1) CYARGDSFC(1) CEPRGDSFC(1) CELRGDSAC(1) CLVRGDSLC(1) CHTRGDTFC(1) CISRGDTFC(1) CVVRGDTFC(1) CEMRGDTFC(1) CDLRGDTYC(1) CVLRGDNFC(1) CVRRGDVFC(1) CGRGDTPTC(1) CRGDTYLIC(1) CLCRGDCIC(1) CRGDCCQSC(1) CLHPNVRSC(1) CDSVLRVFC(1) CQGRGDTFYC(1) CPRRGDTFSC(1) CAHRGDTPQC(1) CVSRGDTPKC(1) CVTRGDSFSC(1) CQVRGDQFAC(1) CTQRGDNFFC(1) CQSRGDDFSC(1) CGRRGDVPRC(1) CRGDTPGFLC(1) CRGDLPRAWC(1) CRGDVPAVGC(1) CYRGDADFWC(1) CSQKRGDTWC(1) CPDRGDKTYC(1) CGPRERFLSC(1) CIRQRIYPWC(1) CGQRSSARAS(1) CGSPLKSIKC(1) CIEIQHGKAC(1) CLESRGPQKC(1) CRKQVMACTA(1) CEAKFQLHWV(1) CVGKELHKRV(1) CTRKRAVGAA(1) RGD 51 Phage display (common panning) 2/3/4 PMID:9634769 Integrin alpha-V-beta-5, integrin αvβ5 Not determined. Not determined. Not determined. CX5C+CX6C+CX7C+CX9 phage display library pool 2658 EFKHSVVGCEALLRYNTPPDPELIC(1) CPRHSIVEAAALLRY(1) SRHSVLEPALLRYSEIQAQSFHG(1) CPRHSIVETAALLRY(1) GRHSVLGPSMALLRY(1) AELRHSVMLGALLRYGAIEPRGKSH(1) SRHSVLAPALLRYEEIIAYSGSS(1) AASTSPGIGAALLRYYQIKKHSLIP(1) SRHSVLAPALLRY(1) VFRHSLVWSQALLRY(1) RHSMVSVDVRALLRY(1) TRHSILRPVNALLRYCIETNNDC(1) SSLSDASMVDALLRYYQIKKHSLIP(1) VHADLHDNTKALLRYYQIKKHSLIP(1) EFKHSVVGCEALLRYHVTHGLT(1) CIMRHSVVPDALLRYAPEDQRQICH(1) VFRHSLVWSQALLRYAPEDQRQICH(1) GRHSVLGPSMALLRY(1) SRHSVLEPALLRYSIIEAHSGTC(1) EFKHSVVGCEALLRYTRSALTSDPC(1) EFKHSVVGCEALLRYGGIAPHRCTC(1) 21 Phage display (common panning) 4 PMID:8755549 Anti-P53 monoclonal antibody PAb240 Not determined. Not determined. Not determined. X10ALLRYX10 phage display library 2659 SISFGQLWRPALLRYTEASNIIRRT(2) SISFGQLWRPALLRLLHKDNAFVR(1) SISFGQLWRPALLRYESHAGSPRGR(1) SISFGQLWRPALLRYTDAVNTSLRI(1) SISFGQLWRPALLRYDHSTTARINL(1) EVRWFDWIHKALLRYPTVLINVRNP(1) SISFGQLWRPALLRYEISPASVRLR(1) 7 Phage display (common panning) 3 PMID:8755549 Alkaline phosphatase, tissue-nonspecific isozyme Not determined. Not determined. Not determined. X10ALLRYX10 phage display library 2660 SISFGQLWRPALLRYGSTPVTLAIS(4) SISFGQLWRPALLRYDVKILHGSQR(2) 2 Phage display (common panning) 4 PMID:8755549 Alkaline phosphatase, tissue-nonspecific isozyme Not determined. Not determined. Not determined. X10ALLRYX10 phage display library 2661 DLMGLRNSVLALLRYKLPKPTPGPN(19) 1 Phage display (common panning) 3-4 PMID:8755549 Alkaline phosphatase, tissue-nonspecific isozyme Not determined. Not determined. Not determined. X10ALLRYX10 phage display library 2662 WYEYGWDETVALLRYIEGTSISNAA(3) 1 Phage display (common panning) 3-4 PMID:8755549 Beta-glucuronidase, GUS Not determined. Not determined. Not determined. X10ALLRYX10 phage display library 2663 DPVTDEWVWEALLRYQQATARILLS(3) 1 Phage display (common panning) 3 PMID:8755549 Beta-glucuronidase, GUS Not determined. Not determined. Not determined. X10ALLRYX10 phage display library 2664 DPLSAFGWNAALLRYKHDIQTVYAQ(4) DPVFYVDVLPALLRYQKIFANNI(3) DPVFYVDVLPALLRYTGSTIPTTIR(2) 3 Phage display (common panning) 4 PMID:8755549 Beta-glucuronidase, GUS Not determined. Not determined. Not determined. X10ALLRYX10 phage display library 2665 CRNPWC 1 Phage display (common panning) PMID:9050835 BCL1 IgM idiotype Not determined. Not determined. Not determined. f3-6mer phage display library (X6) 2666 TAAGLCEFDQ 1 Phage display (common panning) PMID:9050835 BCL1 IgM idiotype Not determined. Not determined. Not determined. X10ALLRYX10 phage display library 2667 MSRPACPNDKYE MSARHVPNDKYE MTSRTSPNDKYE MSRSDVPNDKYE GSRVPWPNDKYE GARLPYPNDKYE SAAARVPNDKYE SQVCRRPNDKYE SFVWRMPNDKYE RMAKPVPNDKYE MSQRIQPNDKYE MTHPVAPNDKYE MHAGELPNDKYE MMIGTNPNDKYE MMISPNDKYE GSRGDWPNDKYE 16 Phage display (common panning) 3 PMID:7990127 Thrombin receptor Not determined. Not determined. Not determined. X6PNDKYEPF phage display library Platelets were mixed with phage particles. After several cycles of washing, the resulting platelet/phage pellet was resuspended in PBS, milk containing SFLLRNPNDKYE (thrombin receptor activating peptide). 2668 ADGAARPIAPGAAG ADGAARTVSPGAAG ADGAARVPSTGAAG ADGASRAFSYGAAG ADGAARMPSAGAAG ADGAARVFLRGAAG ADGASRPVYVGAAG ADGASRMVHVGAAG 8 Phage display (common panning) 3 PMID:7990127 Thrombin receptor Not determined. Not determined. Not determined. f3-6mer phage display library (X6) The real target is thrombin receptor on platelets. Platelets were mixed with phage particles. After several cycles of washing, the resulting platelet/phage pellet was resuspended in PBS, milk containing SFLLRNPNDKYE (thrombin receptor activating peptide). 2669 SVWRWLPYDKYE 1 Phage display (common panning) PMID:9050835 BCL1 IgM idiotype Not determined. Not determined. Not determined. X6PNDKYEPF phage display library 2670 AEWPSLTEIKTLSHFSV AEWPSISTLSTYTHSLH AEWPSIHHMSHLLYSTY AEWPSVRTILNTDLLHP AEWPSPTRVISTTYFGS AEWPSPHKIMSTLQYLR AEWPTTRELRSLKSFLT AEWPKMTALQSTMKYVT AEWPKSFLMWMPKATQL AEWPRLSTLASMTNKAI AEWPRVKDLSTYLEGHV AEPWPMLKQLRLLKSSL AEPMNWPTVHAIRSLRK AEHISWPTLAQMSLMNF AELAHWPPVKTVLRSFT AEVTKWPNLTQLRMLAT AEGSTNRWPTVAKLMST AEPWKLIRDSLQLNYLP AEGSWLQLLNLMKQMNN AEASWTTVRSHFGSTMQ AEQLSWKALVTSVLSPT AELSELWRNYSVRLMSS AEAQLNRWKSLSKTMMS AETPSTSKWHQLIKSHR AEISLPQLMJLTHRLKQ AEFPLLPY7WMARHHGS AENLTPQYFKKWQDLTK AETSSLRNTLQMJRSLI 28 Phage display (competitive panning) 3 PMID:8226817 Calmodulin, CaM Not determined. Not determined. Not determined. X15 M13 phage display library Calmodulin was coupled to CNBr-activated Sepharose 4B (1 mg of protein/ml of resin). An aliquot (e12 pfu) of the random sequence bacteriophage library containing 1 mM CaCl2, was applied to a column of calmodulin-Sepharose. The column was then washed successively with sterile column buffers containing 1 mM CaCl2, then 1 mM CaCl2 and 1 M NaCl, and eluted with the calcium chelator, 1 mM EGTA and 1 M NaCl. The EGTA-eluted bacteriophage were amplified. An aliquot of the amplified bacteriophage was reselected two times. 2671 SSWDVLREAFTSRHPADLVHQADSQLSRASR SSWDTVRERLLKSYTADHSKTPPNRTAISSR SSRWEIVRTGLLTRPAGITNASPPTITESRR SSKWDLLRGVFWEGDRGDALSGTDTHGRTSR SSGWERVRSWAASSSAARNTSVSVTPSDQSR SSSHWDVLRGAVTLPAADSNAGRSWRTSTSR SSSRDHWSMLRGCFSSAGCSYWPDSRSHINSR SSSYALRWDALRDCIAAGCHRTDHYVRSVDSR SRCEADLHWALDRWSAAVKAGGTMPGSACSR SSGDARGSHWGFLRSAVNSSQLINTRSLTSR SSSTSNRTPGWERLRAAVNNGMKSLNDLGPSR SSATGGSTASRWAAARLRSFSPPSVIQSR 12 Phage display (common panning) 3 PMID:8635738 Calmodulin, CaM Not determined. Not determined. Not determined. R26 M13 phage display library (SRX12(A/S/P/T)A(A/D/E/G/V)X12SR) CaM (0.2 μg/well) was immobilized to 96-well polystyrene microtiter plates by non-specific absorption in 0.1 M NaHCO3 (pH 8.5). Twelve CaM-binding peptides contained the sequence motif: +W-OλR where +, -, O and λ are positively charged, negatively charged, hydrophobic, and Leu/Val, respectively. 2672 SSTSSVGRAFDVWRAAVLYSTHAVPPEQSSR SSLGLNSGDRTGWRAAVDQLLRLNKNKFDSR SSAVTDPATRSTKWAAAVADIIRSKNMQKSR WRAAV 3 Phage display (common panning) 3 PMID:8635738 Calmodulin, CaM Not determined. Not determined. Not determined. R26 M13 phage display library (SRX12(A/S/P/T)A(A/D/E/G/V)X12SR) CaM (0.2 μg/well) was immobilized to 96-well polystyrene microtiter plates by non-specific absorption in 0.1 M NaHCO3 (pH 8.5). Biotinylated CaM (0.2 μg/well) was immobilized using streptavidin (0.5 μg/well) absorbed as above. For libraries screening, e11 phage particles were incubated with immobilized CaM, non-binding phage washed away, binding phage recovered by acid elution and amplified in E. coll. Three rounds of screening were performed. 2673 SSSELSSSSRWRGLAAALMGYDTSSQQGPSR SSERDSIGRMWRQSAAALRSSFAHSQTNDSR W-R-x-x-A-A-A-L 2 Phage display (common panning) 3 PMID:8635738 Calmodulin, CaM Not determined. Not determined. Not determined. R26 M13 phage display library (SRX12(A/S/P/T)A(A/D/E/G/V)X12SR) CaM (0.2 μg/well) was immobilized to 96-well polystyrene microtiter plates by non-specific absorption in 0.1 M NaHCO3 (pH 8.5). Biotinylated CaM (0.2 μg/well) was immobilized using streptavidin (0.5 μg/well) absorbed as above. For libraries screening, e11 phage particles were incubated with immobilized CaM, non-binding phage washed away, binding phage recovered by acid elution and amplified in E. coll. Three rounds of screening were performed. 2674 SSADHALGEARRANTADKSSWPSVKRVLHSR SSTPNSELGVYKQYSAANIFRSWASRAASR SRGNGERELWWKAFSAVTEGKIKKAPGHTSR 3 Phage display (common panning) 3 PMID:8635738 Calmodulin, CaM Not determined. Not determined. Not determined. R26 M13 phage display library (SRX12(A/S/P/T)A(A/D/E/G/V)X12SR) CaM (0.2 μg/well) was immobilized to 96-well polystyrene microtiter plates by non-specific absorption in 0.1 M NaHCO3 (pH 8.5). Biotinylated CaM (0.2 μg/well) was immobilized using streptavidin (0.5 μg/well) absorbed as above. For libraries screening, e11 phage particles were incubated with immobilized CaM, non-binding phage washed away, binding phage recovered by acid elution and amplified in E. coll. Three rounds of screening were performed. 2675 CLRSGRGC(12) CLRSGFGC(5) 2 Phage display (subtractive panning) 3 PMID:9261123 Echovirus 22, EV22 Not determined. Not determined. Not determined. CX5C+CX6C+CX7C+CX9 phage display library pool Prior to panning with the virus, the phages were incubated for 1 h at 4°C in BSA-coated wells in 500 ml of Tris-buffered saline supplemented with 1 mg/ml BSA and 0.5 mM MgCl2.