2601 CPYLPSIPC CYTKPYPYC CSPTTGQSC 3 Phage display (subtractive panning) 3 PMID:15200474 Tomato-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The real target is carotenoid. Phages were submitted to deselection on cotton/selection on stained cotton swatches for three times. Bound phages were eluted by the method of 'polymerase chain reaction (PCR)' elution and sequenced after the first round of biopanning. 2602 CSTSLLNAC 1 Phage display (subtractive panning) 5 PMID:15200474 Tomato-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The real target is carotenoid. After deselection on cotton/selection on stained cotton swatches for two times, amplified phages were submitted to another three rounds of biopanning on stained cotton swatches. Bound phages were eluted by the method of 'polymerase chain reaction (PCR)' elution. 2603 CGHSMLTNC CNTSYQYRC 2 Phage display (subtractive panning) 2 PMID:15200474 Tomato-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The real target is carotenoid. Phages were submitted to deselection on cotton/selection on stained cotton swatches for two times. Bound phages were eluted by the method of 'polymerase chain reaction (PCR)' elution and sequenced after the first round of biopanning. 2604 HSFRVTSNLSPP NPTTTYKMTPTM TATHLSPGAWRP SMQLQLIPSTPT 4 Phage display (subtractive panning) 3/5 PMID:15200474 Tomato-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The real target is carotenoid. After deselection on cotton/selection on stained cotton swatches for two times, amplified phages were submitted to another one round or three rounds of biopanning on stained cotton swatches. 2605 KASNLSPILGLP TVLSPLTQTLYF SLPAQPRLTHLW TVLSPLTQTLYF GTYNLPNPPPPL HYSSQSNLADSH EPTTTTLPTVGR SRNIPLPSHFLS 8 Phage display (subtractive panning) 3 PMID:15200474 Tomato-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The real target is carotenoid. Phages were submitted to deselection on cotton/selection on stained cotton swatches for three times. Bound phages were eluted by the method of 'polymerase chain reaction (PCR)' elution and sequenced after the first round of biopanning. 2606 HFAYTKPMRIPQ PWASITPPPLLR SHAITATHLEPS LQLQLLPYAFPV 4 Phage display (subtractive panning) 2 PMID:15200474 Tomato-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The real target is carotenoid. Phages were submitted to deselection on cotton/selection on stained cotton swatches for two times. Bound phages were eluted by the method of 'polymerase chain reaction (PCR)' elution and sequenced after the first round of biopanning. 2607 LNANLPANSVLA LPAQMTPVSVVR 2 Phage display (subtractive panning) 5 PMID:15200474 Tomato-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The real target is carotenoid. After deselection on cotton/selection on stained cotton swatches for two times, amplified phages were submitted to another three rounds of biopanning on stained cotton swatches. Bound phages were eluted by the method of 'polymerase chain reaction (PCR)' elution. 2608 SPLTQYVTPRHP LPAQYQTIPGSL SPLTQYVTPRHP ALGSMTWSPPPL SYTKPDQHALAF VASRLTGSVASA LPAQYQTIPGSL 7 Phage display (subtractive panning) 3/5 PMID:15200474 Paprika-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The real target is carotenoid. After deselection on cotton/selection on stained cotton swatches for two times, amplified phages were submitted to another one round or three rounds of biopanning on stained cotton swatches. 2609 SPHSMLQNPSGP YLPSTFAPPLPL 2 Phage display (subtractive panning) 4 PMID:15200474 Paprika-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The real target is carotenoid. After deselection on cotton/selection on stained cotton swatches for three times, amplified phages were submitted to another one round of biopanning on stained cotton swatches. 2610 AVSLVPPNLATH VASANPHSMTSW KAIGMSTGPLTQ VASANPHSMTSW LHVTTTIPGGLR 5 Phage display (subtractive panning) 2 PMID:15200474 Paprika-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The real target is carotenoid. Phages were submitted to deselection on cotton/selection on stained cotton swatches for two times. Bound phages were eluted by the method of 'polymerase chain reaction (PCR)' elution and sequenced after the first round of biopanning. 2611 CSNLAPMLC CNLATSNAC CSPTAAQSC 3 Phage display (subtractive panning) 4 PMID:15200474 Paprika-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The real target is carotenoid. After deselection on cotton/selection on stained cotton swatches for three times, amplified phages were submitted to another one round of biopanning on stained cotton swatches. 2612 CINTPHSMC 1 Phage display (subtractive panning) 5 PMID:15200474 Paprika-stained cotton swatches Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The real target is carotenoid. After deselection on cotton/selection on stained cotton swatches for two times, amplified phages were submitted to another three rounds of biopanning on stained cotton swatches. Bound phages were eluted by the method of 'polymerase chain reaction (PCR)' elution. 2613 DGVCQCRGDCFCP(4) DGFCECRGDCLCF(2) DGVCQCRGDCLCV(1) DGPCHCRGDCLCQ(1) DGARSCRGDCVEA(1) DGARYCRGDCFDG(1) 6 Phage display (subtractive panning) 3-4 PMID:15235124 Human dermal microvascular endothelial cells, HDMECs Not determined. Not determined. Not determined. CPL4b phage display library (DGXXXCRGDCXXX) HEK293 was used in the negative selection. 2614 DGVCQCRGDCFCP(4) DGECQCRGDCLCP(1) DGFPGCRGDCSQE(1) 3 Phage display (subtractive panning) 3-4 PMID:15235124 Human melanoma cell line MeWo Not determined. Not determined. Not determined. CPL4b phage display library (DGXXXCRGDCXXX) HEK293 was used in the nagative selection. 2615 DGALYCRGDCVDQ(4) DGARVCRGDCVDQ(2) DGEACCRGDCVCE(1) DGSRLCRGDCVDA(1) DGAFLCRGDCVDF(1) 5 Phage display (subtractive panning) 3 PMID:15235124 Mixture of murine melanoma cell lines B16.F1 and B16.F10 Not determined. Not determined. Not determined. CPL4b phage display library (DGXXXCRGDCXXX) NIH 3T3 was used in the negative selection. 2616 LSGCRGDCFQQ(1) LSACRGDCQRV(1) 2 Phage display (subtractive panning) 3 PMID:15235124 Human melanoma cell line MeWo Not determined. Not determined. Not determined. CPL4c phage display library (XXXCRGDCXXX) HEK293 was used in the nagative selection. 2617 LTAELTP(2) AASARLP(1) IVAQPRL(1) QPRLLHH(1) TRSQPPL(1) SHFSHLR(1) SPDHLFC(1) IPMHLHN(1) NHLSALY(1) ETIKTNT(1) TSFFMPP(1) SPSVVPF(1) HFTHPTH(1) SWNHARV(1) EAVPTYS(1) SDLATVR(1) LPTKTLF(1) KIDGTPR(1) WTSDELH(1) SLPRNSD(1) LCMTTLV(1) VYFPAPN(1) FPQTYTT(1) NIIPAPT(1) NTGPNRV(1) AFNYPPH(1) LEHPPTT(1) TSESPTV(1) TYSLKSA(1) ATGFATP(1) TAAYRFW(1) HSLTFSI(1) AGATAMS(1) NHWHGGL(1) INSNAPG(1) INSVSPH(1) AAWPTSS(1) VEPARAS(1) TLGLHMS(1) GYQQVFQ(1) SEVAVQG(1) FSMSTPS(1) NIAAFSL(1) SPTYPRR(1) PPPDWTF(1) DFLQVSP(1) TYPSSEW(1) YSLSRSL(1) TSTMPSR(1) TNSQPSP(1) LPPSLYS(1) WNSTTQA(1) STYPIIR(1) GILSPSH(1) YSTHSTR(1) MSSPGPA(1) SIGYPLP(1) LSNFHSS(1) QPRL, HL, PS, INS 58 Phage display (common panning) PMID:10889136 Human umbilical vein endothelial cells, HUVECs Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) The peptide SIGYPLP retargeted gene delivery specifically to endothelial cells with a significantly enhanced efficiency over nontargeted adenovirus and without transduction of nontarget cells. 2618 SIGYPLP(45) MSPPGPA(33) LSNFHSS(2) 3 Phage display (subtractive panning) 4 PMID:10889136 Human umbilical vein endothelial cells, HUVECs Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Phages were biopanned on HUVECs after biopanning on 2 successive cultures of VSMCs, HepG2 cells, and peripheral blood mononuclear cells (preclearing steps). The peptide SIGYPLP retargeted gene delivery specifically to endothelial cells with a significantly enhanced efficiency over nontargeted adenovirus and without transduction of nontarget cells. 2619 LRGDWSED(1) VRGDWYEY(1) VRGDCSSS(1) GRGDLCDF(1) GRGDSPAS(1) 5 Phage display (common panning) 2 PMID:12595259 Integrin alpha-V-beta-5, integrin αvβ5 Not determined. Not determined. Not determined. JCFN-RGD phage display library (XRGDXXXX) 2620 SRGDVVPP(2) PRGDWIEF(1) GRGDSPAS(1) GRGDDDRL(1) GRGDYVLG(1) GRGDFSFL(1) GRGDWTEH(1) ARGDWVEG(1) PRGDWTEG(1) GRGDAFSL(1) FRGDSPLD(1) TRGDPPPH(1) PRGDWNEG(1) FRGDWIEL(1) 14 Phage display (common panning) 3 PMID:12595259 Integrin alpha-V-beta-5, integrin αvβ5 Not determined. Not determined. Not determined. JCFN-RGD phage display library (XRGDXXXX) 2621 CPGPEGAGC(0.14) 1 Phage display (in vivo) 4 PMID:11854520 Mouse breast tissue Not determined. Not determined. Not determined. T7 CX7C phage display library The phage-displayed peptide library was injected intravenously into mice and subsequently rescued bound phage from breast tissue. 2622 PRPGAPLAGSWPGTS(5)[24.8, 45.0] DRWRPALPVVLFPLH(1)[64.2, 36.1] ASSSYPLIHWRPWAR(1)[53.5, 29.3] AAEWLDAFFVRHVDR(1)[8.0, NT] GDVWLFLTSTSHFAR(1)[5.2, NT] EGCSVSSVGALCTHV(1)[4.8, NT] APCCSHLDASPFQRP(1)[3.6, NT] PAQSNFVTWGYNVAV(1)[2.8, NT] RASDVGSDVVPRYPF(1)[1.5, NT] 9 Phage display (in vivo) 5 PMID:11965539 Angiogenic vessel Not determined. Not determined. Not determined. X15 fUSE5 phage display library Binding assay For affinity screening, B16BL6 cells were implanted subcutaneously into the posterior fank of 5-week-old C57BL/6 male mice. Each sample of phage clones (1.0e11 c.f.u.) was injected into tumor-bearing mice via a tail vein when the tumor size had become about 10 mm in diameter. Four minutes after injection, the phages that had accumulated in tumor tissue were recovered and titrated. Phage affinity was expressed as a fold of the control value obtained with random peptide expressing-phage. Both phage affinity against B16BL6 melanoma and phage affinity against Meth A sarcoma are shown. NT denotes not tested. The phage-displayed peptide library was injected into DAS model mice via a tail vein. Four minutes after injection, the phages that had accumulated in angiogenic vessels were recovered and titrated. Biopanning steps were repeated for five cycles. 2623 GECRENVCMG(10) NTCKRFNCNL(2) 2 Phage display (competitive panning) 3 PMID:9288781 Angiogenin Actin Not determined. Not determined. XXCX4CXX phage display library In the first round, bound phages were eluted by a high concentration of actin (5 μM) following washing with a lower concentration of actin (50 nM). In the second round, bound phages were first eluted with 50 nM actin and then by glycine-HCI, pH 2.2. In the third round, bound phages were eluted by a high concentration of actin (5 μM) following washings with lower concentrations of actin (first 50 nM, second 500 nM). 2624 GECRENVCMG(13/15) 1 Phage display (competitive panning) 3 PMID:9288781 Angiogenin Actin Not determined. Not determined. XXCX4CXX phage display library In the first round, bound phages were eluted by a high concentration of actin (5 μM) following washing with a lower concentration of actin (50 nM). In the second round, bound phages were first eluted with 50 nM actin and then by glycine-HCI, pH 2.2. In the third round, bound phages were eluted by a high concentration of actin (5 μM) following washings with lower concentrations of actin (first 50 nM, second 500 nM) and subsequenctly eluted with glycine-HCI, pH 2.2. After three rounds of panning, 15 phages were harvested. Thirteen clones encode GECRENVCMG, except for two clones that did not yield any identifiable sequence. 2625 VVACSWDWTMGAVVCYERI(1/25) WTGWCLNPEESTWGFCTGSF(1/25) 2 Phage display (subtractive panning) 1 PMID:15300809 Extracellular domain of receptor tyrosine-protein kinase erbB-2 Not determined. Not determined. Not determined. X4CX10CX4 fUSE5 phage display library In the first round, 0.9 μg ECDAP were incubated with anti-AP conjugated agarose beads on a nutator overnight at 4°C. The amplified phage library was incubated with anti-AP conjugated agarose beads alone for 2 hr on a nutator at room temperature to remove those phage specific for AP. The unbound phage were filtered with a 0.45 μm cellulose acetate syringe filter and incubated with ECDAP bound to beads on a nutator for 2 hr at room temperature. The unbound phage were removed by washing 5 times with PBS containing a cocktail of prokaryotic protease inhibitors (PPI) (Sigma). One clone (EC-1, displaying WTGWCLNPEESTWGFCTGSF) bound all preparations of ErbB2 including live cells and fresh frozen human breast cancer specimens. The synthetic peptide based on the deduced sequence of the EC-1 clone and its biotin-conjugated form retained binding affinity for purified ErbB2 and ErbB2 overexpressing cell lysates. EC-1 peptide was able to effectively inhibit the phosphorylation of ErbB2 on residues Y1248 and Y877 in a dose- and time-dependent manner. Furthermore, EC-1 peptide selectively inhibits the proliferation of ErbB2 overexpressing breast cancer cells. The linear portion of the cyclic EC-1 peptide was shown to be essential for binding ErbB2. In addition, 4 biased phage libraries were constructed allowing 4 different regions of the EC-1 peptide to have random sequence.