2551 TYLNTP GYRAAP LYRYHL PTLVNA VRPHQR PFCPYR 6 Phage display (common panning) 5 PMID:7831288 Hairpin DNA containing an altered Zif268 binding site TGG Not determined. Not determined. Not determined. pComb3.5 X6 phage display library 2552 GVTMQP[15.9] REQVSR[6.4] QRMRTL[27.5] LRTGNY[4.6] PQPLSD[NT] THMWMI[NT] QRVGLF[NT] EREFSL[NT] EKESRG[NT] EGVRKN[NT] TGVNSI[NT] TQARPP[NT] THMWMI[NT] 13 Phage display (common panning) 5 PMID:7831288 Hairpin DNA containing an altered Zif268 binding site TTG Not determined. Not determined. Not determined. pComb3.5 X6 phage display library Surface plasmon resonance (SPR) The calculated equilibrium dissociation constants (Kd, nM) of zinc-finger proteins are shown. The value of the wild-type (WT) protein is 83.7. NT denotes not tested. 2553 SSGQWWR[13.1] RSDLANS[NT] SSLLVIA[NT] VSSVRGL[NT] 4 Phage display (common panning) 5 PMID:7831288 Hairpin DNA containing an altered Zif268 binding site GCG Not determined. Not determined. Not determined. pComb3.5 XSX5 phage display library Surface plasmon resonance (SPR) The calculated equilibrium dissociation constants (Kd, nM) of zinc-finger proteins are shown. The value of the wild-type (WT) protein is 6.5. NT denotes not tested. 2554 NSVGDKP SSWICGI ISAWMEL ISMMTFF RSECRML ISALLDT NSVQGLR 7 Phage display (common panning) 5 PMID:7831288 Hairpin DNA containing an altered Zif268 binding site CTG Not determined. Not determined. Not determined. pComb3.5 XSX5 phage display library 2555 SRSDHLIR(2) SRSDHLTR(1) SRSDHLLR(1) SRTDHLTR(1) SRSDHLTT(1) 5 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GGG. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2556 SRSDNLRR(5) 1 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GAG. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2557 ARRDNLQR(1) 1 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H X5LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GAG. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2558 GRHDSLLR(5) ARKDALTR(1) 2 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H X5LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GTG. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2559 SRKDSLVR(1) SRMDSLAR(1) SRLDSLKR(1) SRGDALRR(1) SRSDSLLR(1) 5 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GTG. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2560 SRLDTLGR(5) SRRDTLVR(1) SRKDTLAR(1) SRSDTLRR(1) SRSDTLKR(1) 5 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GCG. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2561 GRADTLRR(3) 1 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H X5LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GCG. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2562 SQRAHLER(3) SQAGHLRR(3) 2 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GGA. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2563 SQRSNLVR(6) 1 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GAA. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2564 SQSSSLVR(2) SERSSLVR(2) SQLSGLKR(1) SQMGSLTR(1) SQRGSLLR(1) 5 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GTA. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2565 SQSGDLRR(4) SQSGDLQR(1) SQKGTLIR(1) SQQGTLRR(1) SRSDHLTT(1) 5 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GCA. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2566 AQSGDLTR(1) AQAGTLMR(1) 2 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H X5LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GCA. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2567 TTADKLSR(3) RTKSSLRR(2) TTTGGLTR(1) 3 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H X5LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GGT. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2568 STQGGLAR(1) STSGSLAR(1) STTGGLAR(1) STSGGLAR(1) STSGGLTR(1) 5 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GGT. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2569 STSGNLVR(2) STEGNLVR(2) STSGNLKR(1) STKGNLAR(1) 4 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GAT. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2570 STSGSLTR(6) STSGALTT(4) SERWMLLR(2) 3 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GTT. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2571 ARRSDLTR(4) 1 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H X5LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GCT. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2572 SSSQTLTR(2) 1 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GCT. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2573 SERSKLAR(6) 1 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GGC. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2574 RDPGNLKR(5) 1 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H X5LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GAC. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads. 2575 SAKRMLGQ(1) 1 Phage display (subtractive panning) 7 PMID:10077584 Hairpin DNA Not determined. Not determined. Not determined. pComb3H SX4LX2 phage display library Hairpin target oligonucleotides had the sequence 5'-Biotin-GGACGCN'N'N'CGCGGGTTTTCCCGCGNNNGCGTCC-3', where NNN was the 3-nt finger-2 target sequence and N'N'N' its complement. The selection target site is GAC. A similar nonbiotinylated oligonucleotide, in which the target sequence was TGG (compTGG), was included at 7.2 nM in every round of selection to select against contaminating parental phage. Two pools of nonbiotinylated oligonucleotides also were used as competitors: one containing all 64 possible 3-nt targets sequences (compNNN), the other containing all of the GNN target sequences except for the current selection target (compGNN). These pools typically were used as follows: round 1, no compNNN or compGNN; round 2, 7.2 nM compGNN; round 3, 10.8 nM compGNN; round 4, 1.8 mM compNNN, 25 nM compGNN; round 5, 2.7 mM compNNN, 90 nM compGNN; round 6, 2.7 mM compNNN, 250 nM compGNN; round 7, 3.6 mM compNNN, 250 nM compGNN. The sheared herring sperm DNA was first incubated with the library, before the addition of 72 nM biotinylated hairpin target oligonucleotide. Each selection was performed on streptavidin-coated magnetic beads.