2526 IWAADWV IWQWEWL VWSVEWV PWMGPEW 4 Phage display (common panning) 3 PMID:17113859 Anti-Lewis X monoclonal antibody MMA Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2527 LWKFMGR IWRWAYF 2 Phage display (common panning) 3 PMID:17113859 Anti-Lewis X monoclonal antibody SSEA-1 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2528 IWLQRRH LYLQRLR MWLQSVH YWLQTVR LWLMKAR 5 Phage display (common panning) 3 PMID:17113859 Anti-Lewis X monoclonal antibody Pmn6 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2529 VHHSCTKLTHCCQNWH(11/25) GGVSCMQTSPVCENNL(6/25) TKRDCSAQNYGCQKAI(2/25) 3 Phage display (subtractive panning) 2 PMID:18723274 Tumor-associated glycoprotein (TAG-72) Not determined. Not determined. Not determined. f88-Cys6 phage display library (X4CX6CX4) To begin one selection cycle, the three coated wells of one plate were washed and then blocked with 0.1 M sodium bicarbonate buffer containing 0.5% BSA. The phage library, containing about 2e8 phages in 400 μl of TBS/Tween and 0.1% BSA, was added first to the well coated with the TAG-72 free eluant from the affinity column. After incubation for 20 min the library was transferred and incubated for 30 min in the BSA coated well. Thereafter, the library was transferred to the final well containing the purified TAG-72 and incubated with gentle mixing for 1 hr at room temperature. 2530 VHHSCTKLTHCCQNWH(0.52) GGVSCMQTSPVCENNL(0.24) 2 Phage display (subtractive panning) 3 PMID:18723274 Tumor-associated glycoprotein (TAG-72) Not determined. Not determined. Not determined. f88-Cys6 phage display library (X4CX6CX4) To begin one selection cycle, the three coated wells of one plate were washed and then blocked with 0.1 M sodium bicarbonate buffer containing 0.5% BSA. The phage library, containing about 2e8 phages in 400 μl of TBS/Tween and 0.1% BSA, was added first to the well coated with the TAG-72 free eluant from the affinity column. After incubation for 20 min the library was transferred and incubated for 30 min in the BSA coated well. Thereafter, the library was transferred to the final well containing the purified TAG-72 and incubated with gentle mixing for 1 hr at room temperature. 2531 RNVPPTFNDVYWIAF VPPCFTLMYCAGVVR V-P(2)-x-F-x(3)-Y 2 Phage display (common panning) 3 PMID:9414130 Anti-Lc4Cer monoclonal antibody AD117m Not determined. Not determined. Not determined. X15 fUSE5 phage display library The antibody was biotinylated by incubation with sulfo-NHS-biotin (Pierce, Rockford, IL). Each round of biopanning was performed on the streptavidin-coated polystyrene Petri dish. 2532 FRSFHYHTGRWHWLR RNVPPIFNDVYWIAF ARFPKELRGSVRSAH 3 Phage display (common panning) 3 PMID:9414130 Anti-nLc4Cer monoclonal antibody H11 Not determined. Not determined. Not determined. X15 fUSE5 phage display library The antibody was biotinylated by incubation with sulfo-NHS-biotin (Pierce, Rockford, IL). Each round of biopanning was performed on the streptavidin-coated polystyrene Petri dish. 2533 FRSDVRFWHWSTPFM(11) VRVYFGFGPPPYFGG(9) WHWRHRIPLQLAAGR(2) RYWLYGDPASFPVNH(2) 4 Phage display (common panning) 4 PMID:9877157 Anti-GD1α monoclonal antibody KA17 Ganglioside GD1α SIAT7-E and SIAT7-F Not determined. Not determined. X15 fUSE5 phage display library Each round of biopanning was performed on the 96-well microtiter well (Falcon 3072) coated with streptavidin. The biotinylated KA17 was adsorbed onto the streptavidin-coated well, and affinity-isolation of phages from the peptide epitope library by four cycles of biopanning was carried out. 2534 ISLLQAR IDLMQAR IILLQAR IELLQAR ISLLGAR FSLLDAR IFLLWQR FAQLDWH 8 Phage display (common panning) 3 PMID:10667600 Anti-Lewis A monoclonal antibody Sialyl Lewis A oligosaccharide Not determined. Not determined. f3-7mer fUSE5-based phage display library A flat-bottomed Linbro/Titertek 96-well plate (ICN Biomedicals) was coated with 10 mg of goat antimouse immunoglobulins, blocked with PBS containing 3% BSA and 0.02% Tween 20, and then incubated with 2-3 mg monoclonal anti-Lewis A antibody (clone 7LE; mouse IgG1, Seikagaku Biochemicals). After washing with PBS containing 0.02% Tween 20, wells were blocked with PBS containing 3% BSA. A linear heptapeptide phage library (1e1-1e12 transducing units) was added to the well and incubated at room temperature for 1 h. After removing unbound phage, phages eluted in the first round were amplified and subjected to another two rounds of paning with anti-Lewis A antibody in the same manner as the first selection. 2535 DRPVPY[765] KSPTPY[641] ARPLWY[583] VRPQVP[524] R-P-x(2)-Y 4 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody SA3 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. X6 phage display library ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector fd-tet without a random peptide insert is 169. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2536 MCPPLYSPSACA[957] ECNFLYPGFTCA[202] C-x(1-2)-L-Y 2 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody Strep 9 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. LX-8 phage display library (XCX8CX) ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 14. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2537 YPYCGHALCPGLYADAS[1020] VILPYDNNCALCLNLYP[644] VIDAPTPNCAWPNGRRG[256] MPPAGTGTCFLYALSCS[153] ADLSPTPYCQPSTMHTN[144] NEYINQDHCLLYAMLCP[38] P-T-P-x-C 6 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody Strep 9 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. X8CX8 f88-based phage display library ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 14. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2538 EIAPQGPSKCLLYAYCQ[13] 1 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody Strep 9 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. X15CX phage display library ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 14. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2539 ADAAPSPTPYLPRLS[643] ATYRPVPAEFARKHL[425] TITATDSPTPWPFER[244] P-x-P-x(1-2)-P 3 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody HGAC 39 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. X15 phage display library ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 72. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2540 MCRPSPYNPPCT[112] 1 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody HGAC 39 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. LX-8 phage display library (XCX8CX) ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 72. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2541 MCRPGIPTHHCA[174] HCSPGQRPGTCQ[165] DCGNMLHAEVCR[149] DCRPGVPLLSCP[115] RPG 4 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody HGAC 47 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. LX-8 phage display library (XCX8CX) ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 87. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2542 SCISAACFCI[141] 1 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody HGAC 101 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. f88-LX6 phage display library (XCX6CX) ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 29. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2543 KQLMAP[138] 1 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody HGAC 101 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. X6 phage display library ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 29. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2544 NYPMDH[141] MYPMSH[104] YPMGHL[27] IYPMPA[13] QQYPMG[12] QSTYPM[10] YPM 6 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody SE155.4 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. X6 phage display library ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 14. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2545 EPYPMSEANYVRPMP[267] YPMPASSDNAQWLLK[15] DGTNAYPMNEDISVS[15] HSTRNYSYLGSPYPM[13] NYPMSGARIEPLLHA[13] YAATEPRYMIPYPMP[13] YPMGETCQRIRSCVW[11] YPM 7 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody SE155.4 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. X15 phage display library ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 14. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2546 VCPAPYPAGTCA[11] 1 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody SE155.4 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. LX-8 phage display library (XCX8CX) ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 14. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2547 YTTQCGYGGCMNFE[938] MGVICMNMECDRNM[799] LHEYCNMETCPYNH[585] QYPQCHNMDCKSIT[472] PTHVCYNMECQGGD[275] TPTNCYNMTCQNQP[187] C-x-N-M-[ED] 6 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody SYAJ/6 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. X4CX4CX4 f88-4-based phage display library ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 66. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2548 MDWNMH[993] 1 Phage display (common panning) 3-4 PMID:9122216 Anti-GAS CWPS monoclonal antibody SYAJ/6 Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS Not determined. Not determined. X6 phage display library ELISA Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 66. The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells. 2549 KSQLPWT[25.0] KSADLKR[0.5] KSCVRGR[6.5] KSCDRGR[NT] KSYCRTR[NT] KSNSQHP[NT] KSCQMDS[NT] QSQVTRT[NT] TSQSQSP[NT] VSHIQAN[NT] 10 Phage display (common panning) 5 PMID:7831288 Hairpin DNA containing an altered Zif268 binding site GCG Not determined. Not determined. Not determined. pComb3.5 XSX5 phage display library Surface plasmon resonance (SPR) The calculated equilibrium dissociation constants (Kd, nM) of zinc-finger proteins are shown. The value of the wild-type (WT) protein is 6.5. NT denotes not tested. 2550 QSTASKA[29.7] PSTHLQT[41.6] PSERTQP[NT] TSSEADH[NT] SSEQRYP[NT] HSQQNKP[NT] RSGQGMA[NT] RSARQTG[NT] ESNSFTD[NT] NSVMGHD[NT] NSRGQRK[NT] SSRPSQW[NT] TSSEADH[NT] 13 Phage display (common panning) 5 PMID:7831288 Hairpin DNA containing an altered Zif268 binding site TGT Not determined. Not determined. Not determined. pComb3.5 XSX5 phage display library Surface plasmon resonance (SPR) The calculated equilibrium dissociation constants (Kd, nM) of zinc-finger proteins are shown. The value of the wild-type (WT) protein is 81.8. NT denotes not tested.