The SABinder tool in the SAROTUP suite is a predictor that can be used to foretell if phages bearing your peptides might bind to streptavidin. If streptavidin is used in your biopanning system to capture the biotinylated target, or to pull down the biotinylated target-phage complexes, you'd better use SABinder to find out possible streptavidin binders and then exclude them from your phage display data.

Pay attention: the threshold to distinguish between predicted positives and negatives (tp) is ranging from 0 to 1. However, it is set to 0.5 by default. That is to say, a peptide will be predicted to be a streptavidin-binding peptide (SBP) if the probability is 0.5 or higher. You can adjust the threshold according to your own needs. For instance, you should set a lower tp if you do not care about false positives and just want to exclude all possible SBPs. On the contrary, you should increase the tp to 0.95, for example, if your purpose is to discover some novel SBPs with greater confidence. Here we go! (Download standalone tools: Windows | Ubuntu);