| Description: | A random 12-peptide library was screened against Erysipelothrix rhusiopthiae and porcine circovirus 2 recombinant Cap protein and the selected peptides were used for detecting the corresponding pathogens quickly and effectively. P1 (WHWNAPWWNGVY) with the characteristics of polystyrene plate (PS) binding target-unrelated peptides (TUPs), was selected from the library. The peptide phage clone was identified as the PS binding TUP phages by phage-ELISA and elution titration. P1 showed strong PS binding affinity which can not be inhibited by usual blocking buffers. In addition, the phage was not propagation-related TUP. |
