| Description: | The peptide PS-tag was obtained after 10 rounds of biopanning selection against the petri dish. Mutant type glutathione S-transferases (GSTs) fused with the selected PS-tag, PS19 (RAFIASRRIKRP), at their C-terminus, GST-PS19, when adsorbed on the PS latex beads had a higher affinity than the wild-type GST, and the specific remaining activity of the immobilized mutant-type GSTs was approximately 10 times higher than that of the wild-type GST. The signal intensity detected for GST-PS19 adsorbed on hydrophilic and hydrophobic PS surfaces using an anti-peptide antibody specific for the N-terminus peptide of GST was much higher than that for the wild-type GST. |
